(Linear) (Six-base) MAPSORT of: KIEE1929.seq Check: 2900 from: 1 to: 1401 KIEE1929.orf With 182 enzymes: SgfI * July 15, 2010 18:51 .. Acc65I G'GTAC_C Cuts at: 0 176 1401 Size: 176 1225 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 456 939 1401 Size: 456 483 462 Fragments arranged by size: 483 462 456 AfeI AGC'GCT Cuts at: 0 1167 1401 Size: 1167 234 AleI CACnn'nnGTG Cuts at: 0 1366 1401 Size: 1366 35 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 661 1401 Size: 661 740 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1108 1140 1401 Size: 1108 32 261 Fragments arranged by size: 1108 261 32 AvaI C'yCGr_G Cuts at: 0 12 310 876 1058 1401 Size: 12 298 566 182 343 Fragments arranged by size: 566 343 298 182 12 BanI G'GyrC_C Cuts at: 0 176 196 1351 1401 Size: 176 20 1155 50 Fragments arranged by size: 1155 176 50 20 BanII G_rGCy'C Cuts at: 0 607 848 1059 1121 1217 1261 1401 Size: 607 241 211 62 96 44 140 Fragments arranged by size: 607 241 211 140 96 62 44 BbsI GAAGACnn'nnnn_ Cuts at: 0 1101 1401 Size: 1101 300 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 975 1009 1401 Size: 975 34 392 Fragments arranged by size: 975 392 34 BglI GCCn_nnn'nGGC Cuts at: 0 353 1401 Size: 353 1048 BglII A'GATC_T Cuts at: 0 212 1401 Size: 212 1189 Bme1580I G_kGCm'C Cuts at: 0 581 909 1356 1401 Size: 581 328 447 45 Fragments arranged by size: 581 447 328 45 BmrI ACTGGGnnnn_n' Cuts at: 0 1264 1401 Size: 1264 137 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 753 1401 Size: 753 648 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 134 539 757 1216 1401 Size: 134 405 218 459 185 Fragments arranged by size: 459 405 218 185 134 BsaAI yAC'GTr Cuts at: 0 904 1130 1401 Size: 904 226 271 Fragments arranged by size: 904 271 226 BsaHI Gr'CG_yC Cuts at: 0 119 227 698 1045 1401 Size: 119 108 471 347 356 Fragments arranged by size: 471 356 347 119 108 BsaWI w'CCGG_w Cuts at: 0 317 1401 Size: 317 1084 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 107 137 157 187 674 704 1108 1138 Size: 107 30 20 30 487 30 404 30 Cuts at: 1138 1401 Size: 263 Fragments arranged by size: 487 404 263 107 30 30 30 30 20 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 416 760 1401 Size: 416 344 641 Fragments arranged by size: 641 416 344 BseYI C'CCAG_C Cuts at: 0 827 975 1048 1401 Size: 827 148 73 353 Fragments arranged by size: 827 353 148 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 631 1221 1401 Size: 631 590 180 Fragments arranged by size: 631 590 180 BsiEI CG_ry'CG Cuts at: 0 366 806 1401 Size: 366 440 595 Fragments arranged by size: 595 440 366 BsiHKAI G_wGCw'C Cuts at: 0 607 1217 1373 1401 Size: 607 610 156 28 Fragments arranged by size: 610 607 156 28 BsmBI CGTCTCn'nnnn_ Cuts at: 0 110 793 1401 Size: 110 683 608 Fragments arranged by size: 683 608 110 Bsp1286I G_dGCh'C Cuts at: 0 581 607 848 909 1059 1121 1217 1261 Size: 581 26 241 61 150 62 96 44 Cuts at: 1261 1356 1373 1401 Size: 95 17 28 Fragments arranged by size: 581 241 150 96 95 62 61 44 28 26 17 BspEI T'CCGG_A Cuts at: 0 317 1401 Size: 317 1084 BsrBI CCG'CTC Cuts at: 0 1200 1401 Size: 1200 201 BssHII G'CGCG_C Cuts at: 0 64 1401 Size: 64 1337 BstXI CCAn_nnnn'nTGG Cuts at: 0 444 1401 Size: 444 957 BstYI r'GATC_y Cuts at: 0 212 320 756 1401 Size: 212 108 436 645 Fragments arranged by size: 645 436 212 108 BtgI C'CryG_G Cuts at: 0 536 741 1089 1401 Size: 536 205 348 312 Fragments arranged by size: 536 348 312 205 BtsI GCAGTG_nn' Cuts at: 0 581 888 1401 Size: 581 307 513 Fragments arranged by size: 581 513 307 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 420 455 1401 Size: 420 35 946 Fragments arranged by size: 946 420 35 EarI CTCTTCn'nnn_ Cuts at: 0 958 1401 Size: 958 443 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 10 1401 Size: 10 1391 EcoICRI GAG'CTC Cuts at: 0 605 1215 1401 Size: 605 610 186 Fragments arranged by size: 610 605 186 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 134 456 539 757 939 1216 1401 Size: 134 322 83 218 182 277 185 Fragments arranged by size: 322 277 218 185 182 134 83 EcoO109I rG'GnC_Cy Cuts at: 0 279 629 948 1027 1401 Size: 279 350 319 79 374 Fragments arranged by size: 374 350 319 279 79 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 156 1401 Size: 156 1245 HaeII r_GCGC'y Cuts at: 0 735 842 1169 1401 Size: 735 107 327 232 Fragments arranged by size: 735 327 232 107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 741 773 1401 Size: 741 32 628 Fragments arranged by size: 741 628 32 HincII GTy'rAC Cuts at: 0 84 1401 Size: 84 1317 KpnI G_GTAC'C Cuts at: 0 180 1401 Size: 180 1221 MslI CAynn'nnrTG Cuts at: 0 442 670 1366 1401 Size: 442 228 696 35 Fragments arranged by size: 696 442 228 35 MspA1I CmG'CkG Cuts at: 0 26 598 827 959 1401 Size: 26 572 229 132 442 Fragments arranged by size: 572 442 229 132 26 NcoI C'CATG_G Cuts at: 0 536 1089 1401 Size: 536 553 312 Fragments arranged by size: 553 536 312 NspI r_CATG'y Cuts at: 0 162 1401 Size: 162 1239 PfoI T'CCnGG_A Cuts at: 0 53 692 1112 1154 1401 Size: 53 639 420 42 247 Fragments arranged by size: 639 420 247 53 42 PmlI CAC'GTG Cuts at: 0 1130 1401 Size: 1130 271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1108 1140 1401 Size: 1108 32 261 Fragments arranged by size: 1108 261 32 PpuMI rG'GwC_Cy Cuts at: 0 279 1027 1401 Size: 279 748 374 Fragments arranged by size: 748 374 279 PshAI GACnn'nnGTC Cuts at: 0 332 1401 Size: 332 1069 PvuII CAG'CTG Cuts at: 0 598 959 1401 Size: 598 361 442 Fragments arranged by size: 598 442 361 SacI G_AGCT'C Cuts at: 0 607 1217 1401 Size: 607 610 184 Fragments arranged by size: 610 607 184 SexAI A'CCwGG_T Cuts at: 0 281 1401 Size: 281 1120 SfcI C'TryA_G Cuts at: 0 616 1401 Size: 616 785 SmaI CCC'GGG Cuts at: 0 1060 1401 Size: 1060 341 SphI G_CATG'C Cuts at: 0 162 1401 Size: 162 1239 StyI C'CwwG_G Cuts at: 0 536 1089 1364 1401 Size: 536 553 275 37 Fragments arranged by size: 553 536 275 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 549 989 1401 Size: 549 440 412 Fragments arranged by size: 549 440 412 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 107 139 157 189 674 706 1401 Size: 107 32 18 32 485 32 695 Fragments arranged by size: 695 485 107 32 32 32 18 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 381 444 1401 Size: 381 63 957 Fragments arranged by size: 957 381 63 XmaI C'CCGG_G Cuts at: 0 1058 1401 Size: 1058 343 Enzymes that do cut: Acc65I AcuI AfeI AleI AlfI AloI AvaI BanI BanII BbsI BcgI BglI BglII Bme1580I BmrI BplI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BsrBI BssHII BstXI BstYI BtgI BtsI CspCI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I HincII KpnI MslI MspA1I NcoI NspI PfoI PmlI PpiI PpuMI PshAI PvuII SacI SexAI SfcI SmaI SphI StyI TaqII TstI XcmI XmaI Enzymes that do not cut: AarI AatII AccI AclI AflII AflIII AgeI AhdI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaBI BsiWI BsmI BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EaeI EagI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I XbaI XhoI XmnI ZraI