Restriction map for
pFN21AE3977
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# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwI AlwNI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BanII BbeI BbrPI BbvCI BciVI
BclI BfaI BfrI BlnI BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmAI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MaeIII MfeI MluI MroI MseI
MunI MvnI NarI NcoI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI TfiI Tru9I Tsp45I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 526 AAATTT [GA]^AATT[CT]
Alw44I 247 GTGCAC G^TGCAC
ApaLI 247 GTGCAC G^TGCAC
ApoI 526 AAATTT [GA]^AATT[CT]
AvaI 556 CCCGAG C^[CT]CG[GA]G
AviII 86 TGCGCA TGC^GCA
BbsI 454 GAAGAC GAAGAC(2/6)
BcgI 311 GCAGCCGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 170 AGATCT A^GATCT
BlpI 703 GCTGAGC GC^T[ACGT]AGC
BpmI 232 CTGGAG CTGGAG(16/14)
Bpu1102I 703 GCTGAGC GC^T[ACGT]AGC
BpuAI 454 GAAGAC GAAGAC(2/6)
BsaBI 510 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 359 CTGCAC GTGCAG(16/14)
BsmI 80 GCATTC GAATGC(1/-1)
BsoBI 556 CCCGAG C^[CT]CG[GA]G
BspMI 484 GCAGGT ACCTGC(4/8)
BssSI 46 CACGAG CACGAG(-5/-1)
BstYI 170 AGATCT [GA]^GATC[CT]
CelII 703 GCTGAGC GC^T[ACGT]AGC
DdeI 704 CTGAG C^T[ACGT]AG
FspI 86 TGCGCA TGC^GCA
HaeII 207 GGCGCT [GA]GCGC^[CT]
HinfI 355 GAGTC G^A[ACGT]TC
MamI 510 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 170 AGATCT [GA]^GATC[CT]
NaeI 98 GCCGGC GCC^GGC
NdeI 379 CATATG CA^TATG
NgoMIV 98 GCCGGC G^CCGGC
NspI 539 ACATGC [GA]CATG^[CT]
PleI 355 GAGTC GAGTC(4/5)
PpuMI 563 GGGACCT [GA]G^G[AT]CC[CT]
PstI 301 CTGCAG CTGCA^G
SexAI 725 ACCAGGT A^CC[AT]GGT
SfcI 301 CTGCAG C^T[GA][CT]AG
SgrAI 97 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 118 CTTGAG C^T[CT][GA]AG
StuI 584 AGGCCT AGG^CCT
TaqI 374 TCGA T^CGA
Tsp509I 527 AATT ^AATT
TthHB8I 374 TCGA T^CGA
XhoII 170 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 69 GTAC GT^AC
537 GTAC
AspHI 247 GTGCAC G[AT]GC[AT]^C
764 GTGCTC
BanI 237 GGTGCC G^G[CT][GA]CC
716 GGCACC
BglI 217 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
621 GCCGGGGCGGC
BsiHKAI 247 GTGCAC G[AT]GC[AT]^C
764 GTGCTC
BsrBI 121 GAGCGG CCGCTC(-3/-3)
559 GAGCGG
BsrFI 98 GCCGGC [GA]^CCGG[CT]
262 ACCGGC
BstF5I 267 CATCC GGATG(2/0)
509 GGATG
Cfr10I 98 GCCGGC [GA]^CCGG[CT]
262 ACCGGC
DpnII 171 GATC ^GATC
376 GATC
DraII 491 AGGCCCC [GA]G^G[ACGT]CC[CT]
563 GGGACCT
DsaI 252 CCACGG C^C[GA][CT]GG
278 CCGTGG
EcoO109I 491 AGGCCCC [GA]G^G[ACGT]CC[CT]
563 GGGACCT
FokI 267 CATCC GGATG(9/13)
509 GGATG
HphI 487 GGTGA GGTGA(8/7)
609 GGTGA
MaeII 551 ACGT A^CGT
677 ACGT
MboI 171 GATC ^GATC
376 GATC
MslI 46 CACGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
546 CACGGACGTG
MspA1I 469 CAGCTG C[AC]G^C[GT]G
709 CAGCTG
NdeII 171 GATC ^GATC
376 GATC
PvuII 469 CAGCTG CAG^CTG
709 CAGCTG
RsaI 69 GTAC GT^AC
537 GTAC
Sau3AI 171 GATC ^GATC
376 GATC
SfaNI 409 GATGC GCATC(5/9)
266 GCATC
XcmI 684 CCACATACTCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
726 CCAGGTGGCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AvaII BalI BbvI
BcnI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstNI
Cac8I CfoI Cfr13I EaeI EarI
EcoRII FauI Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI Ksp632I
MboII MluNI MnlI MscI MspI
MvaI MwoI NciI NlaIII NlaIV
Sau96I ScrFI TseI TspRI