(Circular) (Six-base) MAPSORT of: pFN21AE3977.seq Check: 1946 from: 1 to: 5479 pFN21AE3977 5479 bp With 182 enzymes: SgfI * March 28, 2012 11:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2480 2480 Size: 5479 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3289 278 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2822 1937 Size: 885 4594 Acc65I G'GTAC_C Cuts at: 2800 2800 Size: 5479 AclI AA'CG_TT Cuts at: 1563 3606 3979 1563 Size: 2043 373 3063 Fragments arranged by size: 3063 2043 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2796 3542 4926 5194 1632 Size: 1164 746 1384 268 1917 Fragments arranged by size: 1917 1384 1164 746 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5282 AflIII A'CryG_T Cuts at: 1203 4378 5355 1203 Size: 3175 977 1327 Fragments arranged by size: 3175 1327 977 AgeI A'CCGG_T Cuts at: 4310 4310 Size: 5479 AhdI GACnn_n'nnGTC Cuts at: 4208 4208 Size: 5479 AleI CACnn'nnGTG Cuts at: 1166 2055 2555 1166 Size: 889 500 4090 Fragments arranged by size: 4090 889 500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2073 1184 Size: 889 4590 AlwNI CAG_nnn'CTG Cuts at: 3222 4794 3222 Size: 1572 3907 ApaLI G'TGCA_C Cuts at: 2252 3536 4692 2252 Size: 1284 1156 3039 Fragments arranged by size: 3039 1284 1156 ApoI r'AATT_y Cuts at: 1493 2531 2788 3014 3166 5275 1493 Size: 1038 257 226 152 2109 1697 Fragments arranged by size: 2109 1697 1038 257 226 152 AseI AT'TA_AT Cuts at: 160 1032 4033 160 Size: 872 3001 1606 Fragments arranged by size: 3001 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 AvaI C'yCGr_G Cuts at: 1870 1943 2561 2804 1870 Size: 73 618 243 4545 Fragments arranged by size: 4545 618 243 73 BamHI G'GATC_C Cuts at: 2809 3176 2809 Size: 367 5112 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2242 2721 2800 4255 618 Size: 325 217 627 455 479 79 1455 1842 Fragments arranged by size: 1842 1455 627 479 455 325 217 79 BanII G_rGCy'C Cuts at: 1391 2798 1391 Size: 1407 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2466 928 Size: 971 567 3941 Fragments arranged by size: 3941 971 567 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2305 2339 3670 3704 1903 Size: 34 368 34 1331 34 3678 Fragments arranged by size: 3678 1331 368 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3371 4581 3371 Size: 1210 4269 BclI T'GATC_A Cuts at: 1548 1548 Size: 5479 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2228 2632 4090 Size: 107 122 71 631 605 556 404 1458 Cuts at: 4090 136 Size: 1525 Fragments arranged by size: 1525 1458 631 605 556 404 122 107 71 BglII A'GATC_T Cuts at: 2175 5474 2175 Size: 3299 2180 BlpI GC'TnA_GC Cuts at: 2709 2896 2709 Size: 187 5292 Bme1580I G_kGCm'C Cuts at: 1790 2025 2256 2562 3540 4696 1790 Size: 235 231 306 978 1156 2573 Fragments arranged by size: 2573 1156 978 306 235 231 BmgBI CAC'GTC Cuts at: 1318 2557 1318 Size: 1239 4240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3210 4168 442 Size: 416 53 83 399 24 1793 958 1753 Fragments arranged by size: 1793 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5479 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 4139 1239 Size: 1019 1881 2579 Fragments arranged by size: 2579 1881 1019 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2144 3604 4469 4767 2144 Size: 1460 865 298 2856 Fragments arranged by size: 2856 1460 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4142 882 Size: 617 2643 2219 Fragments arranged by size: 2643 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3270 493 Size: 711 2066 2702 Fragments arranged by size: 2702 2066 711 BsaBI GATnn'nnATC Cuts at: 2519 5473 2519 Size: 2954 2525 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3286 3668 4303 275 Size: 53 83 186 936 1753 382 635 1451 Fragments arranged by size: 1753 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3912 4310 4584 4731 5142 3912 Size: 398 274 147 411 4249 Fragments arranged by size: 4249 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2435 2465 1178 Size: 30 165 30 1032 30 4192 Fragments arranged by size: 4192 1032 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5479 BseYI C'CCAG_C Cuts at: 2669 4682 5178 2669 Size: 2013 496 2970 Fragments arranged by size: 2970 2013 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2349 1163 Size: 573 613 4293 Fragments arranged by size: 4293 613 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2929 3690 4718 1540 Size: 275 186 928 761 1028 2301 Fragments arranged by size: 2301 1028 928 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2256 2773 3540 3625 4696 2256 Size: 517 767 85 1071 3039 Fragments arranged by size: 3039 1071 767 517 85 BsmI GAATG_Cn' Cuts at: 2085 2990 3083 2085 Size: 905 93 4481 Fragments arranged by size: 4481 905 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2256 2562 2773 2798 3540 3625 Size: 399 235 231 306 211 25 742 85 Cuts at: 3625 4696 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 235 231 211 85 25 BspEI T'CCGG_A Cuts at: 5142 5142 Size: 5479 BspHI T'CATG_A Cuts at: 3368 5098 3368 Size: 1730 3749 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2480 2834 844 Size: 1636 354 3489 Fragments arranged by size: 3489 1636 354 BsrBI CCG'CTC Cuts at: 1394 1823 2128 2566 3366 1394 Size: 429 305 438 800 3507 Fragments arranged by size: 3507 800 438 429 305 BsrDI GCAATG_nn' Cuts at: 65 1248 3974 4148 65 Size: 1183 2726 174 1396 Fragments arranged by size: 2726 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2103 2267 4123 4310 1700 Size: 110 293 164 1856 187 2869 Fragments arranged by size: 2869 1856 293 187 164 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5479 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5479 BssSI C'ACGA_G Cuts at: 2051 3539 4551 2051 Size: 1488 1012 2979 Fragments arranged by size: 2979 1488 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5479 BstBI TT'CG_AA Cuts at: 4283 4283 Size: 5479 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5479 BstYI r'GATC_y Cuts at: 1972 2175 2809 3176 3561 3578 5030 5116 5128 Size: 203 634 367 385 17 1452 86 12 Cuts at: 5128 5474 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 634 385 367 346 203 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2257 2283 513 Size: 552 939 253 26 3709 Fragments arranged by size: 3709 939 552 253 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4740 Fragments arranged by size: 4740 683 56 BtsI GCAGTG_nn' Cuts at: 2160 2995 3789 3809 2160 Size: 835 794 20 3830 Fragments arranged by size: 3830 835 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5479 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5444 DraI TTT'AAA Cuts at: 2783 2783 Size: 5479 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4486 809 Size: 507 3170 1802 Fragments arranged by size: 3170 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2129 2220 Size: 54 1425 50 275 18 177 122 91 Cuts at: 2220 2294 2676 2926 3815 5444 8 Size: 74 382 250 889 1629 43 Fragments arranged by size: 1629 1425 889 382 275 250 177 122 91 74 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2926 1537 Size: 275 1114 4090 Fragments arranged by size: 4090 1114 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 2429 2435 2612 3409 1353 Size: 833 243 6 177 797 3423 Fragments arranged by size: 3423 833 797 243 177 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4066 4440 4586 387 Size: 1064 138 2477 374 146 1280 Fragments arranged by size: 2477 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2796 3542 4139 4926 5194 1239 Size: 393 626 538 746 597 787 268 1524 Fragments arranged by size: 1524 787 746 626 597 538 393 268 EcoO109I rG'GnC_Cy Cuts at: 2497 2569 2497 Size: 72 5407 EcoRI G'AATT_C Cuts at: 2788 3166 2788 Size: 378 5101 FspI TGC'GCA Cuts at: 2093 3985 2093 Size: 1892 3587 HaeII r_GCGC'y Cuts at: 1678 2216 4626 1678 Size: 538 2410 2531 Fragments arranged by size: 2531 2410 538 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2435 2467 4120 4152 4194 Size: 32 135 32 863 32 1653 32 42 Cuts at: 4194 4226 1373 Size: 32 2626 Fragments arranged by size: 2626 1653 863 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2823 3069 669 Size: 988 281 885 246 3079 Fragments arranged by size: 3079 988 885 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5479 HpaI GTT'AAC Cuts at: 3069 3069 Size: 5479 KpnI G_GTAC'C Cuts at: 2804 2804 Size: 5479 MfeI C'AATT_G Cuts at: 3078 3078 Size: 5479 MluI A'CGCG_T Cuts at: 5355 5355 Size: 5479 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4593 4777 1305 Size: 3288 184 2007 Fragments arranged by size: 3288 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2222 2296 10 Size: 54 1425 343 177 213 74 3193 Fragments arranged by size: 3193 1425 343 213 177 74 54 MslI CAynn'nnrTG Cuts at: 518 1166 2055 2555 3437 3796 3955 518 Size: 648 889 500 882 359 159 2042 Fragments arranged by size: 2042 889 882 648 500 359 159 MspA1I CmG'CkG Cuts at: 1606 2476 2716 2895 3572 4720 4965 1606 Size: 870 240 179 677 1148 245 2120 Fragments arranged by size: 2120 1148 870 677 245 240 179 NaeI GCC'GGC Cuts at: 2105 2105 Size: 5479 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3988 Fragments arranged by size: 3988 939 552 NdeI CA'TA_TG Cuts at: 387 2385 387 Size: 1998 3481 NgoMIV G'CCGG_C Cuts at: 2103 2103 Size: 5479 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5479 NotI GC'GGCC_GC Cuts at: 2926 2926 Size: 5479 NspI r_CATG'y Cuts at: 1132 1229 2548 2837 4382 1132 Size: 97 1319 289 1545 2229 Fragments arranged by size: 2229 1545 1319 289 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5479 PciI A'CATG_T Cuts at: 4378 4378 Size: 5479 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5479 PfoI T'CCnGG_A Cuts at: 2186 2186 Size: 5479 PmeI GTTT'AAAC Cuts at: 2783 2783 Size: 5479 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3548 3580 5061 5093 3548 Size: 32 1481 32 3934 Fragments arranged by size: 3934 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2569 2569 Size: 5479 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5479 PsiI TTA'TAA Cuts at: 3049 3049 Size: 5479 PstI C_TGCA'G Cuts at: 830 2310 2831 830 Size: 1480 521 3478 Fragments arranged by size: 3478 1480 521 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5479 PvuII CAG'CTG Cuts at: 2476 2716 2476 Size: 240 5239 SalI G'TCGA_C Cuts at: 1936 2821 1936 Size: 885 4594 SbfI CC_TGCA'GG Cuts at: 2831 2831 Size: 5479 ScaI AGT'ACT Cuts at: 3727 5439 3727 Size: 1712 3767 SexAI A'CCwGG_T Cuts at: 2730 2730 Size: 5479 SfcI C'TryA_G Cuts at: 826 1045 2306 2827 3962 4643 4834 826 Size: 219 1261 521 1135 681 191 1471 Fragments arranged by size: 1471 1261 1135 681 521 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5479 SgrAI Cr'CCGG_yG Cuts at: 1700 2103 1700 Size: 403 5076 SmaI CCC'GGG Cuts at: 1872 2806 1872 Size: 934 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2123 3583 4484 4746 820 Size: 197 926 180 1460 901 262 1553 Fragments arranged by size: 1553 1460 926 901 262 197 180 SnaBI TAC'GTA Cuts at: 493 3270 493 Size: 2777 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5479 SphI G_CATG'C Cuts at: 1132 2837 1132 Size: 1705 3774 SspI AAT'ATT Cuts at: 5 52 3403 5 Size: 47 3351 2081 Fragments arranged by size: 3351 2081 47 StuI AGG'CCT Cuts at: 1517 2591 1517 Size: 1074 4405 StyI C'CwwG_G Cuts at: 513 1065 2004 2918 513 Size: 552 939 914 3074 Fragments arranged by size: 3074 939 914 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3506 3523 3676 3861 4306 325 Size: 1255 1926 17 153 185 445 1498 Fragments arranged by size: 1926 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2541 3725 5437 Size: 274 80 33 51 1443 564 1184 1712 Cuts at: 5437 96 Size: 138 Fragments arranged by size: 1712 1443 1184 564 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2182 2214 5439 5471 2182 Size: 32 3225 32 2190 Fragments arranged by size: 3225 2190 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5479 XbaI T'CTAG_A Cuts at: 2815 2815 Size: 5479 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2696 2738 2696 Size: 42 5437 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5479 XmaI C'CCGG_G Cuts at: 1870 2804 1870 Size: 934 4545 XmnI GAAnn'nnTTC Cuts at: 3608 3608 Size: 5479 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3287 276 Size: 53 83 186 936 1753 2468 Fragments arranged by size: 2468 1753 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI