(Linear) (Six-base) MAPSORT of: KIEE3977.seq Check: 8781 from: 1 to: 774 KIEE3977.orf With 182 enzymes: SgfI * March 22, 2012 17:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 475 774 Size: 475 299 AleI CACnn'nnGTG Cuts at: 0 50 550 774 Size: 50 500 224 Fragments arranged by size: 500 224 50 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 68 774 Size: 68 706 ApaLI G'TGCA_C Cuts at: 0 247 774 Size: 247 527 ApoI r'AATT_y Cuts at: 0 526 774 Size: 526 248 AvaI C'yCGr_G Cuts at: 0 556 774 Size: 556 218 BanI G'GyrC_C Cuts at: 0 237 716 774 Size: 237 479 58 Fragments arranged by size: 479 237 58 BbsI GAAGACnn'nnnn_ Cuts at: 0 461 774 Size: 461 313 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 300 334 774 Size: 300 34 440 Fragments arranged by size: 440 300 34 BglI GCCn_nnn'nGGC Cuts at: 0 223 627 774 Size: 223 404 147 Fragments arranged by size: 404 223 147 BglII A'GATC_T Cuts at: 0 170 774 Size: 170 604 BlpI GC'TnA_GC Cuts at: 0 704 774 Size: 704 70 Bme1580I G_kGCm'C Cuts at: 0 20 251 557 774 Size: 20 231 306 217 Fragments arranged by size: 306 231 217 20 BmgBI CAC'GTC Cuts at: 0 552 774 Size: 552 222 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 774 Size: 253 521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 774 Size: 139 635 BsaBI GATnn'nnATC Cuts at: 0 514 774 Size: 514 260 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 430 460 774 Size: 430 30 314 Fragments arranged by size: 430 314 30 BseYI C'CCAG_C Cuts at: 0 664 774 Size: 664 110 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 774 Size: 344 430 BsiHKAI G_wGCw'C Cuts at: 0 251 768 774 Size: 251 517 6 Fragments arranged by size: 517 251 6 BsmI GAATG_Cn' Cuts at: 0 80 774 Size: 80 694 Bsp1286I G_dGCh'C Cuts at: 0 20 251 557 768 774 Size: 20 231 306 211 6 Fragments arranged by size: 306 231 211 20 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 475 774 Size: 475 299 BsrBI CCG'CTC Cuts at: 0 123 561 774 Size: 123 438 213 Fragments arranged by size: 438 213 123 BsrFI r'CCGG_y Cuts at: 0 98 262 774 Size: 98 164 512 Fragments arranged by size: 512 164 98 BssSI C'ACGA_G Cuts at: 0 46 774 Size: 46 728 BstYI r'GATC_y Cuts at: 0 170 774 Size: 170 604 BtgI C'CryG_G Cuts at: 0 252 278 774 Size: 252 26 496 Fragments arranged by size: 496 252 26 BtsI GCAGTG_nn' Cuts at: 0 155 774 Size: 155 619 EaeI y'GGCC_r Cuts at: 0 2 124 215 289 671 774 Size: 2 122 91 74 382 103 Fragments arranged by size: 382 122 103 91 74 2 EarI CTCTTCn'nnn_ Cuts at: 0 181 424 430 607 774 Size: 181 243 6 177 167 Fragments arranged by size: 243 181 177 167 6 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 774 Size: 253 521 EcoO109I rG'GnC_Cy Cuts at: 0 492 564 774 Size: 492 72 210 Fragments arranged by size: 492 210 72 FspI TGC'GCA Cuts at: 0 88 774 Size: 88 686 HaeII r_GCGC'y Cuts at: 0 211 774 Size: 211 563 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 430 462 774 Size: 430 32 312 Fragments arranged by size: 430 312 32 MscI TGG'CCA Cuts at: 0 4 217 291 774 Size: 4 213 74 483 Fragments arranged by size: 483 213 74 4 MslI CAynn'nnrTG Cuts at: 0 50 550 774 Size: 50 500 224 Fragments arranged by size: 500 224 50 MspA1I CmG'CkG Cuts at: 0 471 711 774 Size: 471 240 63 Fragments arranged by size: 471 240 63 NaeI GCC'GGC Cuts at: 0 100 774 Size: 100 674 NdeI CA'TA_TG Cuts at: 0 380 774 Size: 380 394 NgoMIV G'CCGG_C Cuts at: 0 98 774 Size: 98 676 NspI r_CATG'y Cuts at: 0 543 774 Size: 543 231 PfoI T'CCnGG_A Cuts at: 0 181 774 Size: 181 593 PpuMI rG'GwC_Cy Cuts at: 0 564 774 Size: 564 210 PstI C_TGCA'G Cuts at: 0 305 774 Size: 305 469 PvuII CAG'CTG Cuts at: 0 471 711 774 Size: 471 240 63 Fragments arranged by size: 471 240 63 SexAI A'CCwGG_T Cuts at: 0 725 774 Size: 725 49 SfcI C'TryA_G Cuts at: 0 301 774 Size: 301 473 SgrAI Cr'CCGG_yG Cuts at: 0 98 774 Size: 98 676 SmlI C'TyrA_G Cuts at: 0 118 774 Size: 118 656 StuI AGG'CCT Cuts at: 0 586 774 Size: 586 188 TatI w'GTAC_w Cuts at: 0 536 774 Size: 536 238 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 177 209 774 Size: 177 32 565 Fragments arranged by size: 565 177 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 691 733 774 Size: 691 42 41 Fragments arranged by size: 691 42 41 Enzymes that do cut: AarI AleI AlfI ApaLI ApoI AvaI BanI BbsI BcgI BglI BglII BlpI Bme1580I BmgBI BpmI BpuEI BsaBI BsaXI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssSI BstYI BtgI BtsI EaeI EarI Eco57MI EcoO109I FspI HaeII Hin4I MscI MslI MspA1I NaeI NdeI NgoMIV NspI PfoI PpuMI PstI PvuII SexAI SfcI SgrAI SmlI StuI TatI TstI XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AloI AlwNI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbvCI BciVI BclI BfrBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI