Restriction map for
pFN21AE6268
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# NO CUT ENZYMES ( 160 enzymes) #
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AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
AlwNI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstZ17I Bsu36I
CelII CfoI ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I EarI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
Ksp632I KspI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NarI NciI NcoI NdeI NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI StyI
SwaI TfiI Tru9I Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 25 enzymes) #
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Acc65I 161 GGTACC G^GTACC
Asp718I 161 GGTACC G^GTACC
BanII 38 GAGCCC G[GA]GC[CT]^C
BbsI 751 GTCTTC GAAGAC(2/6)
BfaI 80 CTAG C^TAG
BpuAI 751 GTCTTC GAAGAC(2/6)
BseRI 228 CTCCTC GAGGAG(10/8)
BsiEI 678 CGGCCG CG[GA][CT]^CG
BsmBI 390 CGTCTC CGTCTC(1/5)
BsrDI 363 CATTGC GCAATG(2/0)
BstXI 23 CCACTTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 407 AGATCC [GA]^GATC[CT]
EaeI 678 CGGCCG [CT]^GGCC[GA]
EagI 678 CGGCCG C^GGCCG
EclXI 678 CGGCCG C^GGCCG
Eco52I 678 CGGCCG C^GGCCG
EcoNI 516 CCTGCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 9 GACGC GACGC(5/10)
KpnI 161 GGTACC GGTAC^C
MaeI 80 CTAG C^TAG
MflI 407 AGATCC [GA]^GATC[CT]
MslI 24 CACTTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
TaqI 776 TCGA T^CGA
TthHB8I 776 TCGA T^CGA
XhoII 407 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AccII 244 CGCG CG^CG
758 CGCG
AfaI 141 GTAC GT^AC
162 GTAC
Alw44I 319 GTGCAC G^TGCAC
691 GTGCAC
AlwI 408 GATCC GGATC(4/5)
782 GGATC
ApaLI 319 GTGCAC G^TGCAC
691 GTGCAC
AspHI 319 GTGCAC G[AT]GC[AT]^C
691 GTGCAC
BanI 161 GGTACC G^G[CT][GA]CC
436 GGCACC
BsaJI 121 CCCTGG C^C[ACGT][ACGT]GG
720 CCTCGG
BsiHKAI 319 GTGCAC G[AT]GC[AT]^C
691 GTGCAC
BsmFI 53 GGGAC GGGAC(10/14)
180 GTCCC
BstUI 244 CGCG CG^CG
758 CGCG
DdeI 43 CTCAG C^T[ACGT]AG
308 CTAAG
FauI 650 GCGGG CCCGC(4/6)
759 GCGGG
HinfI 280 GAGTC G^A[ACGT]TC
643 GACTC
MaeII 272 ACGT A^CGT
467 ACGT
MaeIII 76 GTGAC ^GT[ACGT]AC
469 GTCAC
MboII 97 GAAGA GAAGA(8/7)
752 TCTTC
MvnI 244 CGCG CG^CG
758 CGCG
NaeI 257 GCCGGC GCC^GGC
433 GCCGGC
NgoMIV 257 GCCGGC G^CCGGC
433 GCCGGC
PflMI 110 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
191 CCAACAGCTGG
PleI 643 GACTC GAGTC(4/5)
280 GAGTC
RsaI 141 GTAC GT^AC
162 GTAC
Tsp45I 76 GTGAC ^GT[GC]AC
469 GTCAC
Tsp509I 769 AATT ^AATT
787 AATT
Van91I 110 CCAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
191 CCAACAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaII BbvI BmyI
BpmI BsaI BsiYI BslI BsmAI
Bsp1286I BspMI BsrFI BsrI BssKI
BstNI Cac8I Cfr10I Cfr13I DpnII
DraII EcoO109I EcoRII Fnu4HI HaeIII
HapII HpaII HphI ItaI MboI
MnlI MspA1I MspI MvaI MwoI
NdeII NlaIV PpuMI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI TspRI