(Circular) (Six-base) MAPSORT of: pFN21AE6268.seq Check: 5225 from: 1 to: 5512 pFN21AE6268 5512 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2183 2567 2683 2183 Size: 384 116 5012 Fragments arranged by size: 5012 384 116 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3322 278 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2855 1937 Size: 918 4594 Acc65I G'GTAC_C Cuts at: 2166 2833 2166 Size: 667 4845 AclI AA'CG_TT Cuts at: 1563 2811 3639 4012 1563 Size: 1248 828 373 3063 Fragments arranged by size: 3063 1248 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3575 4959 5227 1632 Size: 1943 1384 268 1917 Fragments arranged by size: 1943 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5315 AflIII A'CryG_T Cuts at: 1203 4411 5388 1203 Size: 3208 977 1327 Fragments arranged by size: 3208 1327 977 AgeI A'CCGG_T Cuts at: 4343 4343 Size: 5512 AhdI GACnn_n'nnGTC Cuts at: 4241 4241 Size: 5512 AleI CACnn'nnGTG Cuts at: 1166 2033 1166 Size: 867 4645 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5512 AlwNI CAG_nnn'CTG Cuts at: 3255 4827 3255 Size: 1572 3940 ApaLI G'TGCA_C Cuts at: 2324 2696 3569 4725 2324 Size: 372 873 1156 3111 Fragments arranged by size: 3111 1156 873 372 ApoI r'AATT_y Cuts at: 1493 2821 3047 3199 5308 1493 Size: 1328 226 152 2109 1697 Fragments arranged by size: 2109 1697 1328 226 152 AseI AT'TA_AT Cuts at: 160 1032 4066 160 Size: 872 3034 1606 Fragments arranged by size: 3034 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 AvaI C'yCGr_G Cuts at: 1870 1943 2837 1870 Size: 73 894 4545 Fragments arranged by size: 4545 894 73 BamHI G'GATC_C Cuts at: 2842 3209 2842 Size: 367 5145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2166 2441 2833 4288 618 Size: 325 217 627 379 275 392 1455 1842 Fragments arranged by size: 1842 1455 627 392 379 325 275 217 BanII G_rGCy'C Cuts at: 1391 2047 2831 1391 Size: 656 784 4072 Fragments arranged by size: 4072 784 656 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2749 928 Size: 971 850 3691 Fragments arranged by size: 3691 971 850 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3703 3737 1903 Size: 34 1766 34 3678 Fragments arranged by size: 3678 1766 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3404 4614 3404 Size: 1210 4302 BclI T'GATC_A Cuts at: 1548 1548 Size: 5512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4123 136 Size: 107 122 71 631 605 2451 1525 Fragments arranged by size: 2451 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5507 5507 Size: 5512 BlpI GC'TnA_GC Cuts at: 2929 2929 Size: 5512 Bme1580I G_kGCm'C Cuts at: 1790 2328 2700 3573 4729 1790 Size: 538 372 873 1156 2573 Fragments arranged by size: 2573 1156 873 538 372 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5512 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3243 4201 442 Size: 416 53 83 399 24 1826 958 1753 Fragments arranged by size: 1826 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5512 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2308 2308 Size: 5512 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2061 2221 2548 2617 4172 1239 Size: 822 160 327 69 1555 2579 Fragments arranged by size: 2579 1555 822 327 160 69 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3637 4502 4800 3637 Size: 865 298 4349 Fragments arranged by size: 4349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2140 2724 2773 4175 882 Size: 617 641 584 49 1402 2219 Fragments arranged by size: 2219 1402 641 617 584 49 BsaAI yAC'GTr Cuts at: 493 1204 3303 493 Size: 711 2099 2702 Fragments arranged by size: 2702 2099 711 BsaBI GATnn'nnATC Cuts at: 5506 5506 Size: 5512 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3319 3701 4336 275 Size: 53 83 186 936 1786 382 635 1451 Fragments arranged by size: 1786 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3945 4343 4617 4764 5175 3945 Size: 398 274 147 411 4282 Fragments arranged by size: 4282 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2324 2336 2354 2366 2381 Size: 30 165 30 921 12 18 12 15 Cuts at: 2381 2411 2735 2765 1178 Size: 30 324 30 3925 Fragments arranged by size: 3925 921 324 165 30 30 30 30 18 15 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2224 1186 Size: 1038 4474 BseYI C'CCAG_C Cuts at: 2202 2257 2481 2612 4715 5211 2202 Size: 55 224 131 2103 496 2503 Fragments arranged by size: 2503 2103 496 224 131 55 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4939 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2686 2962 3723 4751 1540 Size: 275 186 685 276 761 1028 2301 Fragments arranged by size: 2301 1028 761 685 276 275 186 BsiHKAI G_wGCw'C Cuts at: 2328 2700 3573 3658 4729 2328 Size: 372 873 85 1071 3111 Fragments arranged by size: 3111 1071 873 372 85 BsmI GAATG_Cn' Cuts at: 3023 3116 3023 Size: 93 5419 BsmBI CGTCTCn'nnnn_ Cuts at: 2401 2401 Size: 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2328 2700 2831 3573 3658 4729 Size: 399 257 281 372 131 742 85 1071 Cuts at: 4729 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 372 281 257 131 85 BspEI T'CCGG_A Cuts at: 5175 5175 Size: 5512 BspHI T'CATG_A Cuts at: 3401 5131 3401 Size: 1730 3782 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 2183 2567 2683 2867 844 Size: 1278 61 384 116 184 3489 Fragments arranged by size: 3489 1278 384 184 116 61 BsrBI CCG'CTC Cuts at: 1394 1823 3399 1394 Size: 429 1576 3507 Fragments arranged by size: 3507 1576 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2367 4007 4181 65 Size: 1183 1119 1640 174 1396 Fragments arranged by size: 1640 1396 1183 1119 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2262 2438 2532 2685 4156 4343 1700 Size: 110 452 176 94 153 1471 187 2869 Fragments arranged by size: 2869 1471 452 187 176 153 110 94 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5512 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5512 BssSI C'ACGA_G Cuts at: 3572 4584 3572 Size: 1012 4500 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5512 BstBI TT'CG_AA Cuts at: 4316 4316 Size: 5512 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5512 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2035 Size: 5512 BstYI r'GATC_y Cuts at: 1972 2412 2842 3209 3594 3611 5063 5149 5161 Size: 440 430 367 385 17 1452 86 12 Cuts at: 5161 5507 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 440 430 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4960 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4773 Fragments arranged by size: 4773 683 56 BtsI GCAGTG_nn' Cuts at: 2221 3028 3822 3842 2221 Size: 807 794 20 3891 Fragments arranged by size: 3891 807 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5512 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5477 DraI TTT'AAA Cuts at: 2816 2816 Size: 5512 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4519 809 Size: 507 3203 1802 Fragments arranged by size: 3203 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2683 2959 3848 Size: 54 1425 50 275 18 853 276 889 Cuts at: 3848 5477 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 853 276 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2683 2959 1537 Size: 275 871 276 4090 Fragments arranged by size: 4090 871 276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3442 1353 Size: 2089 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4099 4473 4619 387 Size: 1064 138 2510 374 146 1280 Fragments arranged by size: 2510 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2061 2221 2548 2617 3575 4172 4959 Size: 393 429 160 327 69 958 597 787 Cuts at: 4959 5227 1239 Size: 268 1524 Fragments arranged by size: 1524 958 787 597 429 393 327 268 160 69 EcoNI CCTnn'n_nnAGG Cuts at: 2525 2525 Size: 5512 EcoO109I rG'GnC_Cy Cuts at: 2059 2184 2206 2241 2059 Size: 125 22 35 5330 Fragments arranged by size: 5330 125 35 22 EcoRI G'AATT_C Cuts at: 2821 3199 2821 Size: 378 5134 FspI TGC'GCA Cuts at: 4018 4018 Size: 5512 HaeII r_GCGC'y Cuts at: 1678 4659 1678 Size: 2981 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2324 2356 4153 4185 4227 Size: 32 135 32 752 32 1797 32 42 Cuts at: 4227 4259 1373 Size: 32 2626 Fragments arranged by size: 2626 1797 752 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2856 3102 669 Size: 988 281 918 246 3079 Fragments arranged by size: 3079 988 918 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5512 HpaI GTT'AAC Cuts at: 3102 3102 Size: 5512 KpnI G_GTAC'C Cuts at: 2170 2837 2170 Size: 667 4845 MfeI C'AATT_G Cuts at: 3111 3111 Size: 5512 MluI A'CGCG_T Cuts at: 5388 5388 Size: 5512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2367 4626 4810 1305 Size: 1062 2259 184 2007 Fragments arranged by size: 2259 2007 1062 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3690 Fragments arranged by size: 3690 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2033 3470 3829 3988 518 Size: 648 867 1437 359 159 2042 Fragments arranged by size: 2042 1437 867 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2023 2121 2202 2419 2637 2928 3605 4753 Size: 417 98 81 217 218 291 677 1148 Cuts at: 4753 4998 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 417 291 245 218 217 98 81 NaeI GCC'GGC Cuts at: 2264 2440 2264 Size: 176 5336 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4960 NdeI CA'TA_TG Cuts at: 387 387 Size: 5512 NgoMIV G'CCGG_C Cuts at: 2262 2438 2262 Size: 176 5336 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5512 NotI GC'GGCC_GC Cuts at: 2959 2959 Size: 5512 NspI r_CATG'y Cuts at: 1132 1229 2870 4415 1132 Size: 97 1641 1545 2229 Fragments arranged by size: 2229 1641 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5512 PciI A'CATG_T Cuts at: 4411 4411 Size: 5512 PflMI CCAn_nnn'nTGG Cuts at: 1108 2121 2202 1108 Size: 1013 81 4418 Fragments arranged by size: 4418 1013 81 PfoI T'CCnGG_A Cuts at: 2237 2237 Size: 5512 PmeI GTTT'AAAC Cuts at: 2816 2816 Size: 5512 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3581 3613 5094 5126 3581 Size: 32 1481 32 3967 Fragments arranged by size: 3967 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2059 2184 2206 2241 2059 Size: 125 22 35 5330 Fragments arranged by size: 5330 125 35 22 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5512 PsiI TTA'TAA Cuts at: 3082 3082 Size: 5512 PstI C_TGCA'G Cuts at: 830 2145 2496 2526 2864 830 Size: 1315 351 30 338 3478 Fragments arranged by size: 3478 1315 351 338 30 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5512 PvuII CAG'CTG Cuts at: 2121 2202 2419 2637 2121 Size: 81 217 218 4996 Fragments arranged by size: 4996 218 217 81 SalI G'TCGA_C Cuts at: 1936 2854 1936 Size: 918 4594 SanDI GG'GwC_CC Cuts at: 2059 2184 2059 Size: 125 5387 SbfI CC_TGCA'GG Cuts at: 2864 2864 Size: 5512 ScaI AGT'ACT Cuts at: 3760 5472 3760 Size: 1712 3800 SfcI C'TryA_G Cuts at: 826 1045 2141 2492 2522 2860 3995 4676 4867 Size: 219 1096 351 30 338 1135 681 191 Cuts at: 4867 826 Size: 1471 Fragments arranged by size: 1471 1135 1096 681 351 338 219 191 30 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5512 SmaI CCC'GGG Cuts at: 1872 2839 1872 Size: 967 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3616 4517 4779 820 Size: 197 926 1673 901 262 1553 Fragments arranged by size: 1673 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3303 493 Size: 2810 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5512 SphI G_CATG'C Cuts at: 1132 2870 1132 Size: 1738 3774 SspI AAT'ATT Cuts at: 5 52 3436 5 Size: 47 3384 2081 Fragments arranged by size: 3384 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5512 StyI C'CwwG_G Cuts at: 513 1065 2951 513 Size: 552 1886 3074 Fragments arranged by size: 3074 1886 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3539 3556 3709 3894 4339 325 Size: 1255 1959 17 153 185 445 1498 Fragments arranged by size: 1959 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2145 3758 5470 Size: 274 80 33 51 1443 168 1613 1712 Cuts at: 5470 96 Size: 138 Fragments arranged by size: 1712 1613 1443 274 168 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2735 2767 5472 5504 2735 Size: 32 2705 32 2743 Fragments arranged by size: 2743 2705 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5512 XbaI T'CTAG_A Cuts at: 2848 2848 Size: 5512 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5512 XmaI C'CCGG_G Cuts at: 1870 2837 1870 Size: 967 4545 XmnI GAAnn'nnTTC Cuts at: 3641 3641 Size: 5512 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3320 276 Size: 53 83 186 936 1786 2468 Fragments arranged by size: 2468 1786 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI