################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BalI BanII BbeI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsgI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HincII HindII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MseI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 751 GTCTAC GT^[AC][GT]AC AcyI 619 GGCGTC G[GA]^CG[CT]C AfaI 144 GTAC GT^AC AlwNI 523 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 555 GTGCTC G[AT]GC[AT]^C AviII 353 TGCGCA TGC^GCA BamHI 292 GGATCC G^GATCC BanI 54 GGTGCC G^G[CT][GA]CC BbsI 280 GAAGAC GAAGAC(2/6) BfaI 194 CTAG C^TAG BglII 131 AGATCT A^GATCT BpuAI 280 GAAGAC GAAGAC(2/6) BsaHI 619 GGCGTC G[GA]^CG[CT]C BsaWI 646 TCCGGT [AT]^CCGG[AT] BseRI 95 GAGGAG GAGGAG(10/8) BsiHKAI 555 GTGCTC G[AT]GC[AT]^C BsmI 339 GAATGC GAATGC(1/-1) BstAPI 522 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 407 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 524 AGGGCCT [GA]G^G[ACGT]CC[CT] EaeI 251 TGGCCG [CT]^GGCC[GA] EcoO109I 524 AGGGCCT [GA]G^G[ACGT]CC[CT] FspI 353 TGCGCA TGC^GCA HgaI 620 GCGTC GACGC(5/10) Hin1I 619 GGCGTC G[GA]^CG[CT]C HindIII 376 AAGCTT A^AGCTT MaeI 194 CTAG C^TAG NcoI 596 CCATGG C^CATGG NheI 193 GCTAGC G^CTAGC NspI 387 GCATGT [GA]CATG^[CT] RsaI 144 GTAC GT^AC SmaI 758 CCCGGG CCC^GGG SmlI 148 CTCAAG C^T[CT][GA]AG TaqI 776 TCGA T^CGA TthHB8I 776 TCGA T^CGA XmaCI 758 CCCGGG C^CCGGG XmaI 758 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 158 ACACGT A^C[GA][CT]GT 172 ACGTGT AvaI 83 CCCGAG C^[CT]CG[GA]G 758 CCCGGG AvaII 239 GGACC G^G[AT]CC 491 GGACC BbrPI 159 CACGTG CAC^GTG 552 CACGTG BmyI 55 GTGCCC G[AGT]GC[ACT]^C 555 GTGCTC BsmFI 181 GGGAC GGGAC(10/14) 641 GGGAC BsoBI 83 CCCGAG C^[CT]CG[GA]G 758 CCCGGG Bsp1286I 55 GTGCCC G[AGT]GC[ACT]^C 555 GTGCTC BsrFI 472 ACCGGC [GA]^CCGG[CT] 744 GCCGGT BstYI 131 AGATCT [GA]^GATC[CT] 292 GGATCC Cfr10I 472 ACCGGC [GA]^CCGG[CT] 744 GCCGGT DdeI 397 CTGAG C^T[ACGT]AG 627 CTCAG EcoT14I 449 CCAAGG C^C[AT][AT]GG 596 CCATGG HinfI 436 GACTC G^A[ACGT]TC 643 GACTC HphI 585 GGTGA GGTGA(8/7) 470 TCACC MaeIII 401 GTGAC ^GT[ACGT]AC 469 GTCAC MflI 131 AGATCT [GA]^GATC[CT] 292 GGATCC NlaIII 388 CATG CATG^ 597 CATG PleI 436 GACTC GAGTC(4/5) 643 GACTC PmaCI 159 CACGTG CAC^GTG 552 CACGTG PmlI 159 CACGTG CAC^GTG 552 CACGTG PstI 517 CTGCAG CTGCA^G 529 CTGCAG SfcI 517 CTGCAG C^T[GA][CT]AG 529 CTGCAG StyI 449 CCAAGG C^C[AT][AT]GG 596 CCATGG Tsp45I 401 GTGAC ^GT[GC]AC 469 GTCAC XhoII 131 AGATCT [GA]^GATC[CT] 292 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AluI AlwI BbvI BcnI BpmI BsaAI BsaJI BsiYI BslI BsrDI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII DsaI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIV PvuII Sau3AI Sau96I ScrFI SfaNI TseI TspRI