(Circular) (Six-base) MAPSORT of: pFN21AE5336.seq Check: 8210 from: 1 to: 5509 pFN21AE5336 5509 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3319 278 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2757 2852 1937 Size: 820 95 4594 Fragments arranged by size: 4594 820 95 Acc65I G'GTAC_C Cuts at: 2830 2830 Size: 5509 AclI AA'CG_TT Cuts at: 1563 3636 4009 1563 Size: 2073 373 3063 Fragments arranged by size: 3063 2073 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2569 3572 4956 5224 1632 Size: 937 1003 1384 268 1917 Fragments arranged by size: 1917 1384 1003 937 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5312 AflIII A'CryG_T Cuts at: 1203 2163 2177 4408 5385 1203 Size: 960 14 2231 977 1327 Fragments arranged by size: 2231 1327 977 960 14 AgeI A'CCGG_T Cuts at: 4340 4340 Size: 5509 AhdI GACnn_n'nnGTC Cuts at: 4238 4238 Size: 5509 AleI CACnn'nnGTG Cuts at: 1166 2765 1166 Size: 1599 3910 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5509 AlwNI CAG_nnn'CTG Cuts at: 2533 3252 4824 2533 Size: 719 1572 3218 Fragments arranged by size: 3218 1572 719 ApaLI G'TGCA_C Cuts at: 3566 4722 3566 Size: 1156 4353 ApoI r'AATT_y Cuts at: 1493 2818 3044 3196 5305 1493 Size: 1325 226 152 2109 1697 Fragments arranged by size: 2109 1697 1325 226 152 AseI AT'TA_AT Cuts at: 160 1032 4063 160 Size: 872 3031 1606 Fragments arranged by size: 3031 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 AvaI C'yCGr_G Cuts at: 1870 1943 2088 2763 2834 1870 Size: 73 145 675 71 4545 Fragments arranged by size: 4545 675 145 73 71 BamHI G'GATC_C Cuts at: 2297 2839 3206 2297 Size: 542 367 4600 Fragments arranged by size: 4600 542 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2830 4285 618 Size: 325 217 627 272 771 1455 1842 Fragments arranged by size: 1842 1455 771 627 325 272 217 BanII G_rGCy'C Cuts at: 1391 2828 1391 Size: 1437 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2292 928 Size: 971 393 4145 Fragments arranged by size: 4145 971 393 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3700 3734 1903 Size: 34 1763 34 3678 Fragments arranged by size: 3678 1763 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3401 4611 3401 Size: 1210 4299 BclI T'GATC_A Cuts at: 1548 1548 Size: 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4120 136 Size: 107 122 71 631 605 2448 1525 Fragments arranged by size: 2448 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2136 5504 2136 Size: 3368 2141 BlpI GC'TnA_GC Cuts at: 2926 2926 Size: 5509 Bme1580I G_kGCm'C Cuts at: 1790 2064 3570 4726 1790 Size: 274 1506 1156 2573 Fragments arranged by size: 2573 1506 1156 274 BmgBI CAC'GTC Cuts at: 1318 2329 1318 Size: 1011 4498 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3240 4198 442 Size: 416 53 83 399 24 1823 958 1753 Fragments arranged by size: 1823 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2202 1056 Size: 1146 4363 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2458 2458 Size: 5509 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2032 2129 2297 2548 4169 1239 Size: 793 97 168 251 1621 2579 Fragments arranged by size: 2579 1621 793 251 168 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2138 3634 4499 4797 2138 Size: 1496 865 298 2850 Fragments arranged by size: 2850 1496 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4172 882 Size: 617 2673 2219 Fragments arranged by size: 2673 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2166 2178 2559 3300 493 Size: 711 962 12 381 741 2702 Fragments arranged by size: 2702 962 741 711 381 12 BsaBI GATnn'nnATC Cuts at: 5503 5503 Size: 5509 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2625 3316 3698 4333 Size: 53 83 186 936 1092 691 382 635 Cuts at: 4333 275 Size: 1451 Fragments arranged by size: 1451 1092 936 691 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2651 3942 4340 4614 4761 5172 2651 Size: 1291 398 274 147 411 2988 Fragments arranged by size: 2988 1291 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5284 Fragments arranged by size: 5284 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2115 1186 Size: 929 4580 BseYI C'CCAG_C Cuts at: 2063 2481 2612 4712 5208 2063 Size: 418 131 2100 496 2364 Fragments arranged by size: 2364 2100 496 418 131 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4936 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2959 3720 4748 1540 Size: 275 186 958 761 1028 2301 Fragments arranged by size: 2301 1028 958 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2564 3570 3655 4726 2564 Size: 1006 85 1071 3347 Fragments arranged by size: 3347 1071 1006 85 BsmI GAATG_Cn' Cuts at: 2350 3020 3113 2350 Size: 670 93 4746 Fragments arranged by size: 4746 670 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2564 2828 3570 3655 4726 1391 Size: 399 274 500 264 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 500 399 274 264 85 BspEI T'CCGG_A Cuts at: 5172 5172 Size: 5509 BspHI T'CATG_A Cuts at: 3398 5128 3398 Size: 1730 3779 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2864 844 Size: 2020 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3396 1394 Size: 429 1573 3507 Fragments arranged by size: 3507 1573 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2368 2508 2647 4004 4178 65 Size: 1183 1120 140 139 1357 174 1396 Fragments arranged by size: 1396 1357 1183 1120 174 140 139 BsrFI r'CCGG_y Cuts at: 1700 1810 2477 2749 4153 4340 1700 Size: 110 667 272 1404 187 2869 Fragments arranged by size: 2869 1404 667 272 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5509 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5509 BssSI C'ACGA_G Cuts at: 3569 4581 3569 Size: 1012 4497 BstAPI GCAn_nnn'nTGC Cuts at: 794 2533 794 Size: 1739 3770 BstBI TT'CG_AA Cuts at: 4313 4313 Size: 5509 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5509 BstXI CCAn_nnnn'nTGG Cuts at: 2419 2419 Size: 5509 BstYI r'GATC_y Cuts at: 1972 2136 2297 2839 3206 3591 3608 5060 5146 Size: 164 161 542 367 385 17 1452 86 Cuts at: 5146 5158 5504 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 542 385 367 346 164 161 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2259 2541 2601 513 Size: 552 1194 282 60 3421 Fragments arranged by size: 3421 1194 552 282 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2634 487 Size: 683 56 1408 3362 Fragments arranged by size: 3362 1408 683 56 BtsI GCAGTG_nn' Cuts at: 2214 2224 3025 3819 3839 2214 Size: 10 801 794 20 3884 Fragments arranged by size: 3884 801 794 20 10 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5509 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5474 DraI TTT'AAA Cuts at: 2813 2813 Size: 5509 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4516 809 Size: 507 3200 1802 Fragments arranged by size: 3200 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2256 2956 3845 Size: 54 1425 50 275 18 426 700 889 Cuts at: 3845 5474 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 700 426 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2956 1537 Size: 275 1144 4090 Fragments arranged by size: 4090 1144 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3439 1353 Size: 2086 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2074 2761 4096 4470 4616 387 Size: 1064 138 485 687 1335 374 146 1280 Fragments arranged by size: 1335 1280 1064 687 485 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2032 2129 2297 2548 2569 3572 4169 Size: 393 400 97 168 251 21 1003 597 Cuts at: 4169 4956 5224 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1003 787 597 400 393 268 251 168 97 21 EcoO109I rG'GnC_Cy Cuts at: 2530 2530 Size: 5509 EcoRI G'AATT_C Cuts at: 2818 3196 2818 Size: 378 5131 FspI TGC'GCA Cuts at: 2360 4015 2360 Size: 1655 3854 HaeII r_GCGC'y Cuts at: 1678 4656 1678 Size: 2978 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4150 4182 4224 4256 1373 Size: 32 135 32 2578 32 42 32 2626 Fragments arranged by size: 2626 2578 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2853 3099 669 Size: 988 281 915 246 3079 Fragments arranged by size: 3079 988 915 281 246 HindIII A'AGCT_T Cuts at: 748 2381 748 Size: 1633 3876 HpaI GTT'AAC Cuts at: 3099 3099 Size: 5509 KpnI G_GTAC'C Cuts at: 2834 2834 Size: 5509 MfeI C'AATT_G Cuts at: 3108 3108 Size: 5509 MluI A'CGCG_T Cuts at: 5385 5385 Size: 5509 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2655 4623 4807 1305 Size: 1350 1968 184 2007 Fragments arranged by size: 2007 1968 1350 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3687 Fragments arranged by size: 3687 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2178 2417 2765 3467 3826 3985 518 Size: 648 1012 239 348 702 359 159 2042 Fragments arranged by size: 2042 1012 702 648 359 348 239 159 MspA1I CmG'CkG Cuts at: 1606 2052 2124 2806 2925 3602 4750 4995 1606 Size: 446 72 682 119 677 1148 245 2120 Fragments arranged by size: 2120 1148 682 677 446 245 119 72 NcoI C'CATG_G Cuts at: 513 1065 2601 513 Size: 552 1536 3421 Fragments arranged by size: 3421 1536 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5509 NheI G'CTAG_C Cuts at: 1052 2198 1052 Size: 1146 4363 NotI GC'GGCC_GC Cuts at: 2956 2956 Size: 5509 NspI r_CATG'y Cuts at: 1132 1229 2396 2867 4412 1132 Size: 97 1167 471 1545 2229 Fragments arranged by size: 2229 1545 1167 471 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5509 PciI A'CATG_T Cuts at: 4408 4408 Size: 5509 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5509 PfoI T'CCnGG_A Cuts at: 2564 2564 Size: 5509 PmeI GTTT'AAAC Cuts at: 2813 2813 Size: 5509 PmlI CAC'GTG Cuts at: 2166 2559 2166 Size: 393 5116 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3578 3610 5091 5123 3578 Size: 32 1481 32 3964 Fragments arranged by size: 3964 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5509 PsiI TTA'TAA Cuts at: 3079 3079 Size: 5509 PstI C_TGCA'G Cuts at: 830 2526 2538 2861 830 Size: 1696 12 323 3478 Fragments arranged by size: 3478 1696 323 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5509 PvuII CAG'CTG Cuts at: 2052 2124 2806 2052 Size: 72 682 4755 Fragments arranged by size: 4755 682 72 SalI G'TCGA_C Cuts at: 1936 2851 1936 Size: 915 4594 SbfI CC_TGCA'GG Cuts at: 2861 2861 Size: 5509 ScaI AGT'ACT Cuts at: 3757 5469 3757 Size: 1712 3797 SfcI C'TryA_G Cuts at: 826 1045 2522 2534 2857 3992 4673 4864 826 Size: 219 1477 12 323 1135 681 191 1471 Fragments arranged by size: 1477 1471 1135 681 323 219 191 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5509 SmaI CCC'GGG Cuts at: 1872 2765 2836 1872 Size: 893 71 4545 Fragments arranged by size: 4545 893 71 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 3613 4514 4776 820 Size: 197 926 210 1460 901 262 1553 Fragments arranged by size: 1553 1460 926 901 262 210 197 SnaBI TAC'GTA Cuts at: 493 3300 493 Size: 2807 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5509 SphI G_CATG'C Cuts at: 1132 2867 1132 Size: 1735 3774 SspI AAT'ATT Cuts at: 5 52 3433 5 Size: 47 3381 2081 Fragments arranged by size: 3381 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5509 StyI C'CwwG_G Cuts at: 513 1065 2454 2601 2948 513 Size: 552 1389 147 347 3074 Fragments arranged by size: 3074 1389 552 347 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2300 3536 3553 3706 3891 4336 325 Size: 1255 720 1236 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1236 720 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3755 5467 96 Size: 274 80 33 51 1443 1778 1712 138 Fragments arranged by size: 1778 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2236 2268 5469 5501 2236 Size: 32 3201 32 2244 Fragments arranged by size: 3201 2244 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5509 XbaI T'CTAG_A Cuts at: 2845 2845 Size: 5509 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5509 XmaI C'CCGG_G Cuts at: 1870 2763 2834 1870 Size: 893 71 4545 Fragments arranged by size: 4545 893 71 XmnI GAAnn'nnTTC Cuts at: 3638 3638 Size: 5509 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3317 276 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI