(Linear) (Six-base) MAPSORT of: KIEE5336.seq Check: 3576 from: 1 to: 804 KIEE5336.orf With 182 enzymes: SgfI * January 29, 2014 11:56 .. AccI GT'mk_AC Cuts at: 0 752 804 Size: 752 52 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 564 804 Size: 564 240 AflIII A'CryG_T Cuts at: 0 158 172 804 Size: 158 14 632 Fragments arranged by size: 632 158 14 AleI CACnn'nnGTG Cuts at: 0 760 804 Size: 760 44 AlwNI CAG_nnn'CTG Cuts at: 0 528 804 Size: 528 276 AvaI C'yCGr_G Cuts at: 0 83 758 804 Size: 83 675 46 Fragments arranged by size: 675 83 46 BamHI G'GATC_C Cuts at: 0 292 804 Size: 292 512 BanI G'GyrC_C Cuts at: 0 54 804 Size: 54 750 BbsI GAAGACnn'nnnn_ Cuts at: 0 287 804 Size: 287 517 BglII A'GATC_T Cuts at: 0 131 804 Size: 131 673 Bme1580I G_kGCm'C Cuts at: 0 59 804 Size: 59 745 BmgBI CAC'GTC Cuts at: 0 324 804 Size: 324 480 BmtI G_CTAG'C Cuts at: 0 197 804 Size: 197 607 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 453 804 Size: 453 351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 124 292 543 804 Size: 27 97 168 251 261 Fragments arranged by size: 261 251 168 97 27 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 133 804 Size: 133 671 BsaAI yAC'GTr Cuts at: 0 161 173 554 804 Size: 161 12 381 250 Fragments arranged by size: 381 250 161 12 BsaHI Gr'CG_yC Cuts at: 0 620 804 Size: 620 184 BsaWI w'CCGG_w Cuts at: 0 646 804 Size: 646 158 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 110 804 Size: 110 694 BseYI C'CCAG_C Cuts at: 0 58 476 607 804 Size: 58 418 131 197 Fragments arranged by size: 418 197 131 58 BsiHKAI G_wGCw'C Cuts at: 0 559 804 Size: 559 245 BsmI GAATG_Cn' Cuts at: 0 345 804 Size: 345 459 Bsp1286I G_dGCh'C Cuts at: 0 59 559 804 Size: 59 500 245 Fragments arranged by size: 500 245 59 BsrDI GCAATG_nn' Cuts at: 0 363 503 642 804 Size: 363 140 139 162 Fragments arranged by size: 363 162 140 139 BsrFI r'CCGG_y Cuts at: 0 472 744 804 Size: 472 272 60 Fragments arranged by size: 472 272 60 BstAPI GCAn_nnn'nTGC Cuts at: 0 528 804 Size: 528 276 BstXI CCAn_nnnn'nTGG Cuts at: 0 414 804 Size: 414 390 BstYI r'GATC_y Cuts at: 0 131 292 804 Size: 131 161 512 Fragments arranged by size: 512 161 131 BtgI C'CryG_G Cuts at: 0 254 536 596 804 Size: 254 282 60 208 Fragments arranged by size: 282 254 208 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 629 804 Size: 629 175 BtsI GCAGTG_nn' Cuts at: 0 209 219 804 Size: 209 10 585 Fragments arranged by size: 585 209 10 EaeI y'GGCC_r Cuts at: 0 251 804 Size: 251 553 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 69 756 804 Size: 69 687 48 Fragments arranged by size: 687 69 48 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 124 292 543 564 804 Size: 27 97 168 251 21 240 Fragments arranged by size: 251 240 168 97 27 21 EcoO109I rG'GnC_Cy Cuts at: 0 525 804 Size: 525 279 FspI TGC'GCA Cuts at: 0 355 804 Size: 355 449 HindIII A'AGCT_T Cuts at: 0 376 804 Size: 376 428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 650 804 Size: 650 154 MslI CAynn'nnrTG Cuts at: 0 173 412 760 804 Size: 173 239 348 44 Fragments arranged by size: 348 239 173 44 MspA1I CmG'CkG Cuts at: 0 47 119 801 804 Size: 47 72 682 3 Fragments arranged by size: 682 72 47 3 NcoI C'CATG_G Cuts at: 0 596 804 Size: 596 208 NheI G'CTAG_C Cuts at: 0 193 804 Size: 193 611 NspI r_CATG'y Cuts at: 0 391 804 Size: 391 413 PfoI T'CCnGG_A Cuts at: 0 559 804 Size: 559 245 PmlI CAC'GTG Cuts at: 0 161 554 804 Size: 161 393 250 Fragments arranged by size: 393 250 161 PstI C_TGCA'G Cuts at: 0 521 533 804 Size: 521 12 271 Fragments arranged by size: 521 271 12 PvuII CAG'CTG Cuts at: 0 47 119 801 804 Size: 47 72 682 3 Fragments arranged by size: 682 72 47 3 SfcI C'TryA_G Cuts at: 0 517 529 804 Size: 517 12 275 Fragments arranged by size: 517 275 12 SmaI CCC'GGG Cuts at: 0 760 804 Size: 760 44 SmlI C'TyrA_G Cuts at: 0 148 804 Size: 148 656 StyI C'CwwG_G Cuts at: 0 449 596 804 Size: 449 147 208 Fragments arranged by size: 449 208 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 295 804 Size: 295 509 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 231 263 804 Size: 231 32 541 Fragments arranged by size: 541 231 32 XmaI C'CCGG_G Cuts at: 0 758 804 Size: 758 46 Enzymes that do cut: AccI AcuI AflIII AleI AlwNI AvaI BamHI BanI BbsI BglII Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsiHKAI BsmI Bsp1286I BsrDI BsrFI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EciI Eco57MI EcoO109I FspI HindIII MmeI MslI MspA1I NcoI NheI NspI PfoI PmlI PstI PvuII SfcI SmaI SmlI StyI TaqII TstI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmrI Bpu10I BsaI BsaBI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XhoI XmnI ZraI