Restriction map for
pFN21AE5837
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# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AflII AhdI AocI Aor51HI
ApaI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BcgI
BciVI BfrI BlnI BsaBI BsiEI
BsiWI BsmI BsrDI BssHII BstBI
BstEII BstPI Bsu36I EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
FseI FspI HpaI KspI MamI
MfeI MluI MunI NheI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshBI PvuI SacII SexAI
SfiI SfuI SgrAI SnaBI SwaI
Tth111I XhoI
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# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 51 GGTACC G^GTACC
AccII 390 CGCG CG^CG
AccIII 78 TCCGGA T^CCGGA
AcyI 1229 GGCGCC G[GA]^CG[CT]C
AgeI 4904 ACCGGT A^CCGGT
Alw44I 3654 GTGCAC G^TGCAC
ApaLI 3654 GTGCAC G^TGCAC
Asp718I 51 GGTACC G^GTACC
AvaI 3136 CCCGGG C^[CT]CG[GA]G
BbeI 1229 GGCGCC GGCGC^C
BbrPI 4834 CACGTG CAC^GTG
BclI 1367 TGATCA T^GATCA
BglI 4197 GCCCAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1229 GGCGCC G[GA]^CG[CT]C
BsaI 37 GAGACC GGTCTC(1/5)
BseAI 78 TCCGGA T^CCGGA
BsmBI 1494 GAGACG CGTCTC(1/5)
BsoBI 3136 CCCGGG C^[CT]CG[GA]G
Bsp1407I 1658 TGTACA T^GTACA
BspDI 2695 ATCGAT AT^CGAT
BspEI 78 TCCGGA T^CCGGA
BspHI 1370 TCATGA T^CATGA
BsrGI 1658 TGTACA T^GTACA
BssSI 3664 CACGAG CACGAG(-5/-1)
Bst1107I 217 GTATAC GTA^TAC
BstUI 390 CGCG CG^CG
BstXI 2521 CCAGTCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 217 GTATAC GTA^TAC
ClaI 2695 ATCGAT AT^CGAT
CpoI 2781 CGGTCCG CG^G[AT]CCG
DraIII 4005 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1969 GACCCCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3291 GATATC GAT^ATC
FbaI 1367 TGATCA T^GATCA
HgaI 1128 GCGTC GACGC(5/10)
Hin1I 1229 GGCGCC G[GA]^CG[CT]C
HindIII 3888 AAGCTT A^AGCTT
KasI 1229 GGCGCC G^GCGCC
KpnI 51 GGTACC GGTAC^C
MroI 78 TCCGGA T^CCGGA
MvnI 390 CGCG CG^CG
NaeI 669 GCCGGC GCC^GGC
NarI 1229 GGCGCC GG^CGCC
NdeI 3730 CATATG CA^TATG
NgoMIV 669 GCCGGC G^CCGGC
PinAI 4904 ACCGGT A^CCGGT
PmaCI 4834 CACGTG CAC^GTG
PmlI 4834 CACGTG CAC^GTG
RcaI 1370 TCATGA T^CATGA
RsrII 2781 CGGTCCG CG^G[AT]CCG
SalI 4024 GTCGAC G^TCGAC
SbfI 2107 CCTGCAGG CCTGCA^GG
ScaI 1178 AGTACT AGT^ACT
SfoI 1229 GGCGCC GGC^GCC
SmaI 3136 CCCGGG CCC^GGG
SpeI 1738 ACTAGT A^CTAGT
Sse8387I 2107 CCTGCAGG CCTGCA^GG
SspBI 1658 TGTACA T^GTACA
StuI 1557 AGGCCT AGG^CCT
XbaI 4921 TCTAGA T^CTAGA
XmaCI 3136 CCCGGG C^CCGGG
XmaI 3136 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccI 217 GTATAC GT^[AC][GT]AC
4024 GTCGAC
AclI 1068 AACGTT AA^CGTT
1315 AACGTT
Asp700I 1864 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5025 GAAGGACTTC
BsaAI 2619 TACGTG [CT]AC^GT[GA]
4834 CACGTG
BspLU11I 60 ACATGT A^CATGT
221 ACATGT
BsrBI 1225 GAGCGG CCGCTC(-3/-3)
4927 GAGCGG
EcoRI 1374 GAATTC G^AATTC
2590 GAATTC
EcoT22I 1135 ATGCAT ATGCA^T
4259 ATGCAT
FauI 4607 CCCGC CCCGC(4/6)
3677 GCGGG
NcoI 1973 CCATGG C^CATGG
3929 CCATGG
NsiI 1135 ATGCAT ATGCA^T
4259 ATGCAT
PshAI 2517 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2776 GACCTCGGTC
Psp1406I 1068 AACGTT AA^CGTT
1315 AACGTT
SspI 1457 AATATT AAT^ATT
3970 AATATT
XmnI 1864 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5025 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII