(Circular) (Six-base) MAPSORT of: pFN21AE5837.seq Check: 6167 from: 1 to: 9751 pFN21AE5837 9751 bp With 182 enzymes: SgfI * December 8, 2014 13:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3028 3946 4667 4858 6517 3028 Size: 918 721 191 1659 6262 Fragments arranged by size: 6262 1659 918 721 191 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7561 278 Size: 53 83 186 936 6025 2468 Fragments arranged by size: 6025 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2223 6030 7094 1937 Size: 286 3807 1064 4594 Fragments arranged by size: 4594 3807 1064 286 Acc65I G'GTAC_C Cuts at: 2056 7072 2056 Size: 5016 4735 AclI AA'CG_TT Cuts at: 1563 3074 3321 7878 8251 1563 Size: 1511 247 4557 373 3063 Fragments arranged by size: 4557 3063 1511 373 247 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2594 2637 2967 4605 5496 5823 6290 6415 Size: 962 43 330 1638 891 327 467 125 Cuts at: 6415 6640 6947 7021 7049 7814 9198 9466 1632 Size: 225 307 74 28 765 1384 268 1917 Fragments arranged by size: 1917 1638 1384 962 891 765 467 330 327 307 268 225 125 74 43 28 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9554 AflIII A'CryG_T Cuts at: 1203 2065 2226 3612 4625 8650 9627 1203 Size: 862 161 1386 1013 4025 977 1327 Fragments arranged by size: 4025 1386 1327 1013 977 862 161 AgeI A'CCGG_T Cuts at: 6909 8582 6909 Size: 1673 8078 AhdI GACnn_n'nnGTC Cuts at: 8480 8480 Size: 9751 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9751 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4263 1184 Size: 3079 6672 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4423 4455 4423 Size: 32 9719 AlwNI CAG_nnn'CTG Cuts at: 3971 4130 4474 4778 6977 7027 7494 9066 3971 Size: 159 344 304 2199 50 467 1572 4656 Fragments arranged by size: 4656 2199 1572 467 344 304 159 50 ApaLI G'TGCA_C Cuts at: 5659 7808 8964 5659 Size: 2149 1156 6446 Fragments arranged by size: 6446 2149 1156 ApoI r'AATT_y Cuts at: 1493 2168 2248 2338 2911 3379 3409 3723 4595 Size: 675 80 90 573 468 30 314 872 Cuts at: 4595 5498 5600 5959 7060 7286 7438 9547 1493 Size: 903 102 359 1101 226 152 2109 1697 Fragments arranged by size: 2109 1697 1101 903 872 675 573 468 359 314 226 152 102 90 80 30 AseI AT'TA_AT Cuts at: 160 1032 8305 160 Size: 872 7273 1606 Fragments arranged by size: 7273 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9751 AvaI C'yCGr_G Cuts at: 1870 1943 5141 7076 1870 Size: 73 3198 1935 4545 Fragments arranged by size: 4545 3198 1935 73 BamHI G'GATC_C Cuts at: 7081 7448 7081 Size: 367 9384 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2056 2707 3234 7072 8527 Size: 325 217 627 269 651 527 3838 1455 Cuts at: 8527 618 Size: 1842 Fragments arranged by size: 3838 1842 1455 651 627 527 325 269 217 BanII G_rGCy'C Cuts at: 1391 2412 2498 3576 3935 4394 4742 4796 5121 Size: 1021 86 1078 359 459 348 54 325 Cuts at: 5121 5352 6231 6613 7070 1391 Size: 231 879 382 457 4072 Fragments arranged by size: 4072 1078 1021 879 459 457 382 359 348 325 231 86 54 BbeI G_GCGC'C Cuts at: 3238 3238 Size: 9751 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2439 2582 2643 2880 3006 3995 928 Size: 971 540 143 61 237 126 989 6684 Fragments arranged by size: 6684 989 971 540 237 143 126 61 BbvCI CC'TCA_GC Cuts at: 2052 2659 4506 2052 Size: 607 1847 7297 Fragments arranged by size: 7297 1847 607 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7942 7976 1903 Size: 34 6005 34 3678 Fragments arranged by size: 6005 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 7643 8853 7643 Size: 1210 8541 BclI T'GATC_A Cuts at: 1548 3372 1548 Size: 1824 7927 BfrBI ATG'CAT Cuts at: 3142 6266 3142 Size: 3124 6627 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6208 8362 136 Size: 107 122 71 631 605 4536 2154 1525 Fragments arranged by size: 4536 2154 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2201 3871 4549 5446 5638 9746 2201 Size: 1670 678 897 192 4108 2206 Fragments arranged by size: 4108 2206 1670 897 678 192 BlpI GC'TnA_GC Cuts at: 4767 6230 6807 7168 4767 Size: 1463 577 361 7350 Fragments arranged by size: 7350 1463 577 361 Bme1580I G_kGCm'C Cuts at: 1790 5663 7812 8968 1790 Size: 3873 2149 1156 2573 Fragments arranged by size: 3873 2573 2149 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9751 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3345 4221 5347 Size: 416 53 83 399 24 1928 876 1126 Cuts at: 5347 6107 7482 8440 442 Size: 760 1375 958 1753 Fragments arranged by size: 1928 1753 1375 1126 958 876 760 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9751 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6379 6379 Size: 9751 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2669 2697 3333 3567 3798 4509 5297 6715 Size: 1430 28 636 234 231 711 788 1418 Cuts at: 6715 8411 1239 Size: 1696 2579 Fragments arranged by size: 2579 1696 1430 1418 788 711 636 234 231 28 Bpu10I CC'TnA_GC Cuts at: 2052 2323 2659 4506 6619 2052 Size: 271 336 1847 2113 5184 Fragments arranged by size: 5184 2113 1847 336 271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2687 3149 4177 7006 7876 8741 9039 2687 Size: 462 1028 2829 870 865 298 3399 Fragments arranged by size: 3399 2829 1028 870 865 462 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2036 8414 882 Size: 617 537 6378 2219 Fragments arranged by size: 6378 2219 617 537 BsaAI yAC'GTr Cuts at: 493 1204 4626 6841 7542 493 Size: 711 3422 2215 701 2702 Fragments arranged by size: 3422 2702 2215 711 701 BsaBI GATnn'nnATC Cuts at: 9745 9745 Size: 9751 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3235 7558 7940 8575 Size: 53 83 186 936 1702 4323 382 635 Cuts at: 8575 275 Size: 1451 Fragments arranged by size: 4323 1702 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2083 4877 6909 8184 8582 8856 9003 9414 2083 Size: 2794 2032 1275 398 274 147 411 2420 Fragments arranged by size: 2794 2420 2032 1275 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3306 3336 4204 4234 4729 Size: 30 165 30 1903 30 868 30 495 Cuts at: 4729 4759 1178 Size: 30 6170 Fragments arranged by size: 6170 1903 868 495 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2420 2825 2828 2831 2840 2843 3653 4212 Size: 1234 405 3 3 9 3 810 559 Cuts at: 4212 4881 5158 5173 5176 5203 5223 5798 6447 Size: 669 277 15 3 27 20 575 649 Cuts at: 6447 6637 6703 6706 1186 Size: 190 66 3 4231 Fragments arranged by size: 4231 1234 810 669 649 575 559 405 277 190 66 27 20 15 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3263 3576 5714 8954 9450 3263 Size: 313 2138 3240 496 3564 Fragments arranged by size: 3564 3240 2138 496 313 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3924 4637 5528 5992 6821 1163 Size: 573 2188 713 891 464 829 4093 Fragments arranged by size: 4093 2188 891 829 713 573 464 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 7201 7962 8990 1540 Size: 275 186 5200 761 1028 2301 Fragments arranged by size: 5200 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2412 2498 5663 6231 7812 7897 8968 2412 Size: 86 3165 568 1581 85 1071 3195 Fragments arranged by size: 3195 3165 1581 1071 568 86 85 BsmI GAATG_Cn' Cuts at: 7262 7355 7262 Size: 93 9658 BsmBI CGTCTCn'nnnn_ Cuts at: 3493 3493 Size: 9751 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2412 2498 3576 3935 4394 4742 4796 Size: 399 622 86 1078 359 459 348 54 Cuts at: 4796 5121 5352 5663 6231 6613 7070 7812 7897 Size: 325 231 311 568 382 457 742 85 Cuts at: 7897 8968 1391 Size: 1071 2174 Fragments arranged by size: 2174 1078 1071 742 622 568 459 457 399 382 359 348 325 311 231 86 85 54 BspEI T'CCGG_A Cuts at: 2083 9414 2083 Size: 7331 2420 BspHI T'CATG_A Cuts at: 3375 7640 9370 3375 Size: 4265 1730 3756 Fragments arranged by size: 4265 3756 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3028 3946 4667 4858 6517 7106 844 Size: 2184 918 721 191 1659 589 3489 Fragments arranged by size: 3489 2184 1659 918 721 589 191 BsrBI CCG'CTC Cuts at: 1394 1823 3232 6934 7638 1394 Size: 429 1409 3702 704 3507 Fragments arranged by size: 3702 3507 1409 704 429 BsrDI GCAATG_nn' Cuts at: 65 1248 8246 8420 65 Size: 1183 6998 174 1396 Fragments arranged by size: 6998 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2674 3216 6909 8395 8582 1700 Size: 110 864 542 3693 1486 187 2869 Fragments arranged by size: 3693 2869 1486 864 542 187 110 BsrGI T'GTAC_A Cuts at: 96 3663 96 Size: 3567 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9751 BssSI C'ACGA_G Cuts at: 5669 7811 8823 5669 Size: 2142 1012 6597 Fragments arranged by size: 6597 2142 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2729 4458 5746 6583 6977 794 Size: 1935 1729 1288 837 394 3568 Fragments arranged by size: 3568 1935 1729 1288 837 394 BstBI TT'CG_AA Cuts at: 8555 8555 Size: 9751 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9751 BstXI CCAn_nnnn'nTGG Cuts at: 4533 4533 Size: 9751 BstYI r'GATC_y Cuts at: 1972 2201 3289 3871 4549 4774 4909 5446 5638 Size: 229 1088 582 678 225 135 537 192 Cuts at: 5638 5710 5755 5854 7081 7448 7833 7850 9302 Size: 72 45 99 1227 367 385 17 1452 Cuts at: 9302 9388 9400 9746 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1227 1088 678 582 537 385 367 346 229 225 192 135 99 86 72 45 17 12 BstZ17I GTA'TAC Cuts at: 2224 2224 Size: 9751 BtgI C'CryG_G Cuts at: 513 1065 3978 5112 5934 513 Size: 552 2913 1134 822 4330 Fragments arranged by size: 4330 2913 1134 822 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5528 487 Size: 683 56 4302 4710 Fragments arranged by size: 4710 4302 683 56 BtsI GCAGTG_nn' Cuts at: 3941 4038 4459 7267 8061 8081 3941 Size: 97 421 2808 794 20 5611 Fragments arranged by size: 5611 2808 794 421 97 20 ClaI AT'CG_AT Cuts at: 1554 4701 1554 Size: 3147 6604 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3169 3204 529 Size: 35 2605 35 7076 Fragments arranged by size: 7076 2605 35 35 DraI TTT'AAA Cuts at: 2157 2385 5899 7055 2157 Size: 228 3514 1156 4853 Fragments arranged by size: 4853 3514 1156 228 DraIII CAC_nnn'GTG Cuts at: 6015 6015 Size: 9751 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3980 8758 809 Size: 507 2664 4778 1802 Fragments arranged by size: 4778 2664 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2676 2694 Size: 54 1425 50 275 18 204 642 18 Cuts at: 2694 6747 6789 7198 8087 9716 8 Size: 4053 42 409 889 1629 43 Fragments arranged by size: 4053 1629 1425 889 642 409 275 204 54 50 43 42 18 18 EagI C'GGCC_G Cuts at: 1537 1812 7198 1537 Size: 275 5386 4090 Fragments arranged by size: 5386 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2325 2460 2637 2802 2817 3042 3420 3564 Size: 972 135 177 165 15 225 378 144 Cuts at: 3564 4921 4992 5005 5176 5194 5221 5340 5775 Size: 1357 71 13 171 18 27 119 435 Cuts at: 5775 5799 6083 7681 1353 Size: 24 284 1598 3423 Fragments arranged by size: 3423 1598 1357 972 435 378 284 225 177 171 165 144 135 119 71 27 24 18 15 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4947 8338 8712 8858 387 Size: 1064 138 2636 722 3391 374 146 1280 Fragments arranged by size: 3391 2636 1280 1064 722 374 146 138 EcoICRI GAG'CTC Cuts at: 2410 2496 6229 2410 Size: 86 3733 5932 Fragments arranged by size: 5932 3733 86 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2594 2637 2669 2697 2967 3333 3567 Size: 393 962 43 32 28 270 366 234 Cuts at: 3567 3798 4509 4605 5297 5496 5823 6290 6415 Size: 231 711 96 692 199 327 467 125 Cuts at: 6415 6640 6715 6947 7021 7049 7814 8411 9198 Size: 225 75 232 74 28 765 597 787 Cuts at: 9198 9466 1239 Size: 268 1524 Fragments arranged by size: 1524 962 787 765 711 692 597 467 393 366 327 270 268 234 232 231 225 199 125 96 75 74 43 32 28 28 EcoNI CCTnn'n_nnAGG Cuts at: 2051 2687 4195 6721 2051 Size: 636 1508 2526 5081 Fragments arranged by size: 5081 2526 1508 636 EcoO109I rG'GnC_Cy Cuts at: 2627 3856 4148 5694 6623 2627 Size: 1229 292 1546 929 5755 Fragments arranged by size: 5755 1546 1229 929 292 EcoRI G'AATT_C Cuts at: 3379 4595 7060 7438 3379 Size: 1216 2465 378 5692 Fragments arranged by size: 5692 2465 1216 378 EcoRV GAT'ATC Cuts at: 5298 5298 Size: 9751 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2114 3011 3581 6320 6355 6656 2114 Size: 897 570 2739 35 301 5209 Fragments arranged by size: 5209 2739 897 570 301 35 FspI TGC'GCA Cuts at: 8257 8257 Size: 9751 HaeII r_GCGC'y Cuts at: 1678 2481 3238 7017 8898 1678 Size: 803 757 3779 1881 2531 Fragments arranged by size: 3779 2531 1881 803 757 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2582 2614 3490 3522 3755 Size: 32 135 32 1010 32 876 32 233 Cuts at: 3755 3787 4019 4051 4204 4236 4729 4761 5339 Size: 32 232 32 153 32 493 32 578 Cuts at: 5339 5371 5839 5859 5871 5891 8392 8424 8466 Size: 32 468 20 12 20 2501 32 42 Cuts at: 8466 8498 1373 Size: 32 2626 Fragments arranged by size: 2626 2501 1010 876 578 493 468 233 232 153 135 42 32 32 32 32 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2116 3092 6031 7095 7341 669 Size: 988 281 178 976 2939 1064 246 3079 Fragments arranged by size: 3079 2939 1064 988 976 281 246 178 HindIII A'AGCT_T Cuts at: 748 5893 748 Size: 5145 4606 HpaI GTT'AAC Cuts at: 7341 7341 Size: 9751 KasI G'GCGC_C Cuts at: 3234 3234 Size: 9751 KpnI G_GTAC'C Cuts at: 2060 7076 2060 Size: 5016 4735 MfeI C'AATT_G Cuts at: 7350 7350 Size: 9751 MluI A'CGCG_T Cuts at: 9627 9627 Size: 9751 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2850 5174 6211 8865 9049 1305 Size: 1545 2324 1037 2654 184 2007 Fragments arranged by size: 2654 2324 2007 1545 1037 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2696 6749 6791 10 Size: 54 1425 343 864 4053 42 2970 Fragments arranged by size: 4053 2970 1425 864 343 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 4351 4575 4661 5020 7709 8068 8227 Size: 648 3185 224 86 359 2689 359 159 Cuts at: 8227 518 Size: 2042 Fragments arranged by size: 3185 2689 2042 648 359 359 224 159 86 MspA1I CmG'CkG Cuts at: 1606 2453 4488 4885 6435 6557 6967 6974 7167 Size: 847 2035 397 1550 122 410 7 193 Cuts at: 7167 7844 8992 9237 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 2035 1550 1148 847 677 410 397 245 193 122 7 NaeI GCC'GGC Cuts at: 2676 2676 Size: 9751 NarI GG'CG_CC Cuts at: 3235 3235 Size: 9751 NcoI C'CATG_G Cuts at: 513 1065 3978 5934 513 Size: 552 2913 1956 4330 Fragments arranged by size: 4330 2913 1956 552 NdeI CA'TA_TG Cuts at: 387 5736 387 Size: 5349 4402 NgoMIV G'CCGG_C Cuts at: 2674 2674 Size: 9751 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9751 NotI GC'GGCC_GC Cuts at: 7198 7198 Size: 9751 NsiI A_TGCA'T Cuts at: 3144 6268 3144 Size: 3124 6627 NspI r_CATG'y Cuts at: 1132 1229 2069 2230 2902 3142 4666 6586 7109 Size: 97 840 161 672 240 1524 1920 523 Cuts at: 7109 8654 1132 Size: 1545 2229 Fragments arranged by size: 2229 1920 1545 1524 840 672 523 240 161 97 PasI CC'CwG_GG Cuts at: 1348 5340 1348 Size: 3992 5759 PciI A'CATG_T Cuts at: 2065 2226 8650 2065 Size: 161 6424 3166 Fragments arranged by size: 6424 3166 161 PflMI CCAn_nnn'nTGG Cuts at: 1108 2183 2692 4277 1108 Size: 1075 509 1585 6582 Fragments arranged by size: 6582 1585 1075 509 PfoI T'CCnGG_A Cuts at: 2685 2685 Size: 9751 PmeI GTTT'AAAC Cuts at: 7055 7055 Size: 9751 PmlI CAC'GTG Cuts at: 6841 6841 Size: 9751 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7820 7852 9333 9365 7820 Size: 32 1481 32 8206 Fragments arranged by size: 8206 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2627 3856 4148 2627 Size: 1229 292 8230 Fragments arranged by size: 8230 1229 292 PshAI GACnn'nnGTC Cuts at: 1613 4526 4785 1613 Size: 2913 259 6579 Fragments arranged by size: 6579 2913 259 PsiI TTA'TAA Cuts at: 7321 7321 Size: 9751 PstI C_TGCA'G Cuts at: 830 2623 4117 4671 4726 4860 7103 830 Size: 1793 1494 554 55 134 2243 3478 Fragments arranged by size: 3478 2243 1793 1494 554 134 55 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9751 PvuII CAG'CTG Cuts at: 2453 4885 6435 6967 6974 2453 Size: 2432 1550 532 7 5230 Fragments arranged by size: 5230 2432 1550 532 7 RsrII CG'GwC_CG Cuts at: 4787 4787 Size: 9751 SacI G_AGCT'C Cuts at: 2412 2498 6231 2412 Size: 86 3733 5932 Fragments arranged by size: 5932 3733 86 SalI G'TCGA_C Cuts at: 1936 6029 7093 1936 Size: 4093 1064 4594 Fragments arranged by size: 4594 4093 1064 SapI GCTCTTCn'nnn_ Cuts at: 2325 2460 3564 5340 5799 2325 Size: 135 1104 1776 459 6277 Fragments arranged by size: 6277 1776 1104 459 135 SbfI CC_TGCA'GG Cuts at: 4117 7103 4117 Size: 2986 6765 ScaI AGT'ACT Cuts at: 3185 7999 9711 3185 Size: 4814 1712 3225 Fragments arranged by size: 4814 3225 1712 SfcI C'TryA_G Cuts at: 826 1045 2619 3275 4113 4667 4710 4722 4856 Size: 219 1574 656 838 554 43 12 134 Cuts at: 4856 7099 8234 8915 9106 826 Size: 2243 1135 681 191 1471 Fragments arranged by size: 2243 1574 1471 1135 838 681 656 554 219 191 134 43 12 SfoI GGC'GCC Cuts at: 3236 3236 Size: 9751 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9751 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9751 SmaI CCC'GGG Cuts at: 1872 5143 7078 1872 Size: 3271 1935 4545 Fragments arranged by size: 4545 3271 1935 SmlI C'TyrA_G Cuts at: 820 1017 1943 2666 3128 4192 6985 7855 8756 Size: 197 926 723 462 1064 2793 870 901 Cuts at: 8756 9018 820 Size: 262 1553 Fragments arranged by size: 2793 1553 1064 926 901 870 723 462 262 197 SnaBI TAC'GTA Cuts at: 493 7542 493 Size: 7049 2702 SpeI A'CTAG_T Cuts at: 152 3743 152 Size: 3591 6160 SphI G_CATG'C Cuts at: 1132 2902 4666 6586 7109 1132 Size: 1770 1764 1920 523 3774 Fragments arranged by size: 3774 1920 1770 1764 523 SspI AAT'ATT Cuts at: 5 52 3464 5977 7675 5 Size: 47 3412 2513 1698 2081 Fragments arranged by size: 3412 2513 2081 1698 47 StuI AGG'CCT Cuts at: 1517 3564 1517 Size: 2047 7704 StyI C'CwwG_G Cuts at: 513 1065 3978 5934 6885 7190 513 Size: 552 2913 1956 951 305 3074 Fragments arranged by size: 3074 2913 1956 951 552 305 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3620 4310 4775 5034 5661 6644 7778 Size: 1255 2040 690 465 259 627 983 1134 Cuts at: 7778 7795 7948 8133 8578 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2040 1498 1255 1134 983 690 627 465 445 259 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3183 3663 6289 Size: 274 80 33 51 1443 1206 480 2626 Cuts at: 6289 7997 9709 96 Size: 1708 1712 138 Fragments arranged by size: 2626 1712 1708 1443 1206 480 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 9711 9743 9711 Size: 32 9719 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9751 XbaI T'CTAG_A Cuts at: 6926 7087 6926 Size: 161 9590 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2044 2253 5110 6148 2044 Size: 209 2857 1038 5647 Fragments arranged by size: 5647 2857 1038 209 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9751 XmaI C'CCGG_G Cuts at: 1870 5141 7076 1870 Size: 3271 1935 4545 Fragments arranged by size: 4545 3271 1935 XmnI GAAnn'nnTTC Cuts at: 3873 7034 7880 3873 Size: 3161 846 5744 Fragments arranged by size: 5744 3161 846 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7559 276 Size: 53 83 186 936 6025 2468 Fragments arranged by size: 6025 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BsiWI Bsu36I FseI FspAI NruI PacI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SrfI SwaI