(Linear) (Six-base) MAPSORT of: KIEE5837.seq Check: 4157 from: 1 to: 5046 KIEE5837.orf With 182 enzymes: SgfI * December 5, 2014 13:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1023 1941 2662 2853 4512 5046 Size: 1023 918 721 191 1659 534 Fragments arranged by size: 1659 1023 918 721 534 191 AccI GT'mk_AC Cuts at: 0 218 4025 5046 Size: 218 3807 1021 Fragments arranged by size: 3807 1021 218 Acc65I G'GTAC_C Cuts at: 0 51 5046 Size: 51 4995 AclI AA'CG_TT Cuts at: 0 1069 1316 5046 Size: 1069 247 3730 Fragments arranged by size: 3730 1069 247 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 589 632 962 2600 3491 3818 4285 4410 Size: 589 43 330 1638 891 327 467 125 Cuts at: 4410 4635 4942 5016 5044 5046 Size: 225 307 74 28 2 Fragments arranged by size: 1638 891 589 467 330 327 307 225 125 74 43 28 2 AflIII A'CryG_T Cuts at: 0 60 221 1607 2620 5046 Size: 60 161 1386 1013 2426 Fragments arranged by size: 2426 1386 1013 161 60 AgeI A'CCGG_T Cuts at: 0 4904 5046 Size: 4904 142 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2258 5046 Size: 2258 2788 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2418 2450 5046 Size: 2418 32 2596 Fragments arranged by size: 2596 2418 32 AlwNI CAG_nnn'CTG Cuts at: 0 1966 2125 2469 2773 4972 5022 5046 Size: 1966 159 344 304 2199 50 24 Fragments arranged by size: 2199 1966 344 304 159 50 24 ApaLI G'TGCA_C Cuts at: 0 3654 5046 Size: 3654 1392 ApoI r'AATT_y Cuts at: 0 163 243 333 906 1374 1404 1718 2590 Size: 163 80 90 573 468 30 314 872 Cuts at: 2590 3493 3595 3954 5046 Size: 903 102 359 1092 Fragments arranged by size: 1092 903 872 573 468 359 314 163 102 90 80 30 AvaI C'yCGr_G Cuts at: 0 3136 5046 Size: 3136 1910 BanI G'GyrC_C Cuts at: 0 51 702 1229 5046 Size: 51 651 527 3817 Fragments arranged by size: 3817 651 527 51 BanII G_rGCy'C Cuts at: 0 407 493 1571 1930 2389 2737 2791 3116 Size: 407 86 1078 359 459 348 54 325 Cuts at: 3116 3347 4226 4608 5046 Size: 231 879 382 438 Fragments arranged by size: 1078 879 459 438 407 382 359 348 325 231 86 54 BbeI G_GCGC'C Cuts at: 0 1233 5046 Size: 1233 3813 BbsI GAAGACnn'nnnn_ Cuts at: 0 434 577 638 875 1001 1990 5046 Size: 434 143 61 237 126 989 3056 Fragments arranged by size: 3056 989 434 237 143 126 61 BbvCI CC'TCA_GC Cuts at: 0 47 654 2501 5046 Size: 47 607 1847 2545 Fragments arranged by size: 2545 1847 607 47 BclI T'GATC_A Cuts at: 0 1367 5046 Size: 1367 3679 BfrBI ATG'CAT Cuts at: 0 1137 4261 5046 Size: 1137 3124 785 Fragments arranged by size: 3124 1137 785 BglI GCCn_nnn'nGGC Cuts at: 0 4203 5046 Size: 4203 843 BglII A'GATC_T Cuts at: 0 196 1866 2544 3441 3633 5046 Size: 196 1670 678 897 192 1413 Fragments arranged by size: 1670 1413 897 678 196 192 BlpI GC'TnA_GC Cuts at: 0 2762 4225 4802 5046 Size: 2762 1463 577 244 Fragments arranged by size: 2762 1463 577 244 Bme1580I G_kGCm'C Cuts at: 0 3658 5046 Size: 3658 1388 BmrI ACTGGGnnnn_n' Cuts at: 0 1340 2216 3342 4102 5046 Size: 1340 876 1126 760 944 Fragments arranged by size: 1340 1126 944 876 760 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4374 5046 Size: 4374 672 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 664 692 1328 1562 1793 2504 3292 4710 Size: 664 28 636 234 231 711 788 1418 Cuts at: 4710 5046 Size: 336 Fragments arranged by size: 1418 788 711 664 636 336 234 231 28 Bpu10I CC'TnA_GC Cuts at: 0 47 318 654 2501 4614 5046 Size: 47 271 336 1847 2113 432 Fragments arranged by size: 2113 1847 432 336 271 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 682 1144 2172 5001 5046 Size: 682 462 1028 2829 45 Fragments arranged by size: 2829 1028 682 462 45 BsaI GGTCTCn'nnnn_ Cuts at: 0 31 5046 Size: 31 5015 BsaAI yAC'GTr Cuts at: 0 2621 4836 5046 Size: 2621 2215 210 Fragments arranged by size: 2621 2215 210 BsaHI Gr'CG_yC Cuts at: 0 1230 5046 Size: 1230 3816 BsaWI w'CCGG_w Cuts at: 0 78 2872 4904 5046 Size: 78 2794 2032 142 Fragments arranged by size: 2794 2032 142 78 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1301 1331 2199 2229 2724 2754 5046 Size: 1301 30 868 30 495 30 2292 Fragments arranged by size: 2292 1301 868 495 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 415 820 823 826 835 838 1648 2207 Size: 415 405 3 3 9 3 810 559 Cuts at: 2207 2876 3153 3168 3171 3198 3218 3793 4442 Size: 669 277 15 3 27 20 575 649 Cuts at: 4442 4632 4698 4701 5046 Size: 190 66 3 345 Fragments arranged by size: 810 669 649 575 559 415 405 345 277 190 66 27 20 15 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 1258 1571 3709 5046 Size: 1258 313 2138 1337 Fragments arranged by size: 2138 1337 1258 313 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1919 2632 3523 3987 4816 5046 Size: 1919 713 891 464 829 230 Fragments arranged by size: 1919 891 829 713 464 230 BsiHKAI G_wGCw'C Cuts at: 0 407 493 3658 4226 5046 Size: 407 86 3165 568 820 Fragments arranged by size: 3165 820 568 407 86 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1488 5046 Size: 1488 3558 Bsp1286I G_dGCh'C Cuts at: 0 407 493 1571 1930 2389 2737 2791 3116 Size: 407 86 1078 359 459 348 54 325 Cuts at: 3116 3347 3658 4226 4608 5046 Size: 231 311 568 382 438 Fragments arranged by size: 1078 568 459 438 407 382 359 348 325 311 231 86 54 BspEI T'CCGG_A Cuts at: 0 78 5046 Size: 78 4968 BspHI T'CATG_A Cuts at: 0 1370 5046 Size: 1370 3676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1023 1941 2662 2853 4512 5046 Size: 1023 918 721 191 1659 534 Fragments arranged by size: 1659 1023 918 721 534 191 BsrBI CCG'CTC Cuts at: 0 1227 4929 5046 Size: 1227 3702 117 Fragments arranged by size: 3702 1227 117 BsrFI r'CCGG_y Cuts at: 0 669 1211 4904 5046 Size: 669 542 3693 142 Fragments arranged by size: 3693 669 542 142 BsrGI T'GTAC_A Cuts at: 0 1658 5046 Size: 1658 3388 BssSI C'ACGA_G Cuts at: 0 3664 5046 Size: 3664 1382 BstAPI GCAn_nnn'nTGC Cuts at: 0 724 2453 3741 4578 4972 5046 Size: 724 1729 1288 837 394 74 Fragments arranged by size: 1729 1288 837 724 394 74 BstXI CCAn_nnnn'nTGG Cuts at: 0 2528 5046 Size: 2528 2518 BstYI r'GATC_y Cuts at: 0 196 1284 1866 2544 2769 2904 3441 3633 Size: 196 1088 582 678 225 135 537 192 Cuts at: 3633 3705 3750 3849 5046 Size: 72 45 99 1197 Fragments arranged by size: 1197 1088 678 582 537 225 196 192 135 99 72 45 BstZ17I GTA'TAC Cuts at: 0 219 5046 Size: 219 4827 BtgI C'CryG_G Cuts at: 0 1973 3107 3929 5046 Size: 1973 1134 822 1117 Fragments arranged by size: 1973 1134 1117 822 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 3523 5046 Size: 3523 1523 BtsI GCAGTG_nn' Cuts at: 0 1936 2033 2454 5046 Size: 1936 97 421 2592 Fragments arranged by size: 2592 1936 421 97 ClaI AT'CG_AT Cuts at: 0 2696 5046 Size: 2696 2350 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1164 1199 5046 Size: 1164 35 3847 Fragments arranged by size: 3847 1164 35 DraI TTT'AAA Cuts at: 0 152 380 3894 5046 Size: 152 228 3514 1152 Fragments arranged by size: 3514 1152 228 152 DraIII CAC_nnn'GTG Cuts at: 0 4010 5046 Size: 4010 1036 DrdI GACnn_nn'nnGTC Cuts at: 0 1975 5046 Size: 1975 3071 EaeI y'GGCC_r Cuts at: 0 29 671 689 4742 4784 5046 Size: 29 642 18 4053 42 262 Fragments arranged by size: 4053 642 262 42 29 18 EarI CTCTTCn'nnn_ Cuts at: 0 320 455 632 797 812 1037 1415 1559 Size: 320 135 177 165 15 225 378 144 Cuts at: 1559 2916 2987 3000 3171 3189 3216 3335 3770 Size: 1357 71 13 171 18 27 119 435 Cuts at: 3770 3794 4078 5046 Size: 24 284 968 Fragments arranged by size: 1357 968 435 378 320 284 225 177 171 165 144 135 119 71 27 24 18 15 13 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2220 2942 5046 Size: 2220 722 2104 Fragments arranged by size: 2220 2104 722 EcoICRI GAG'CTC Cuts at: 0 405 491 4224 5046 Size: 405 86 3733 822 Fragments arranged by size: 3733 822 405 86 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 589 632 664 692 962 1328 1562 1793 Size: 589 43 32 28 270 366 234 231 Cuts at: 1793 2504 2600 3292 3491 3818 4285 4410 4635 Size: 711 96 692 199 327 467 125 225 Cuts at: 4635 4710 4942 5016 5044 5046 Size: 75 232 74 28 2 Fragments arranged by size: 711 692 589 467 366 327 270 234 232 231 225 199 125 96 75 74 43 32 28 28 2 EcoNI CCTnn'n_nnAGG Cuts at: 0 46 682 2190 4716 5046 Size: 46 636 1508 2526 330 Fragments arranged by size: 2526 1508 636 330 46 EcoO109I rG'GnC_Cy Cuts at: 0 622 1851 2143 3689 4618 5046 Size: 622 1229 292 1546 929 428 Fragments arranged by size: 1546 1229 929 622 428 292 EcoRI G'AATT_C Cuts at: 0 1374 2590 5046 Size: 1374 1216 2456 Fragments arranged by size: 2456 1374 1216 EcoRV GAT'ATC Cuts at: 0 3293 5046 Size: 3293 1753 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 109 1006 1576 4315 4350 4651 5046 Size: 109 897 570 2739 35 301 395 Fragments arranged by size: 2739 897 570 395 301 109 35 HaeII r_GCGC'y Cuts at: 0 476 1233 5012 5046 Size: 476 757 3779 34 Fragments arranged by size: 3779 757 476 34 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 577 609 1485 1517 1750 1782 2014 2046 Size: 577 32 876 32 233 32 232 32 Cuts at: 2046 2199 2231 2724 2756 3334 3366 3834 3854 Size: 153 32 493 32 578 32 468 20 Cuts at: 3854 3866 3886 5046 Size: 12 20 1160 Fragments arranged by size: 1160 876 578 577 493 468 233 232 153 32 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 0 111 1087 4026 5046 Size: 111 976 2939 1020 Fragments arranged by size: 2939 1020 976 111 HindIII A'AGCT_T Cuts at: 0 3888 5046 Size: 3888 1158 KasI G'GCGC_C Cuts at: 0 1229 5046 Size: 1229 3817 KpnI G_GTAC'C Cuts at: 0 55 5046 Size: 55 4991 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 845 3169 4206 5046 Size: 845 2324 1037 840 Fragments arranged by size: 2324 1037 845 840 MscI TGG'CCA Cuts at: 0 691 4744 4786 5046 Size: 691 4053 42 260 Fragments arranged by size: 4053 691 260 42 MslI CAynn'nnrTG Cuts at: 0 2346 2570 2656 3015 5046 Size: 2346 224 86 359 2031 Fragments arranged by size: 2346 2031 359 224 86 MspA1I CmG'CkG Cuts at: 0 448 2483 2880 4430 4552 4962 4969 5046 Size: 448 2035 397 1550 122 410 7 77 Fragments arranged by size: 2035 1550 448 410 397 122 77 7 NaeI GCC'GGC Cuts at: 0 671 5046 Size: 671 4375 NarI GG'CG_CC Cuts at: 0 1230 5046 Size: 1230 3816 NcoI C'CATG_G Cuts at: 0 1973 3929 5046 Size: 1973 1956 1117 Fragments arranged by size: 1973 1956 1117 NdeI CA'TA_TG Cuts at: 0 3731 5046 Size: 3731 1315 NgoMIV G'CCGG_C Cuts at: 0 669 5046 Size: 669 4377 NsiI A_TGCA'T Cuts at: 0 1139 4263 5046 Size: 1139 3124 783 Fragments arranged by size: 3124 1139 783 NspI r_CATG'y Cuts at: 0 64 225 897 1137 2661 4581 5046 Size: 64 161 672 240 1524 1920 465 Fragments arranged by size: 1920 1524 672 465 240 161 64 PasI CC'CwG_GG Cuts at: 0 3335 5046 Size: 3335 1711 PciI A'CATG_T Cuts at: 0 60 221 5046 Size: 60 161 4825 Fragments arranged by size: 4825 161 60 PflMI CCAn_nnn'nTGG Cuts at: 0 178 687 2272 5046 Size: 178 509 1585 2774 Fragments arranged by size: 2774 1585 509 178 PfoI T'CCnGG_A Cuts at: 0 680 5046 Size: 680 4366 PmlI CAC'GTG Cuts at: 0 4836 5046 Size: 4836 210 PpuMI rG'GwC_Cy Cuts at: 0 622 1851 2143 5046 Size: 622 1229 292 2903 Fragments arranged by size: 2903 1229 622 292 PshAI GACnn'nnGTC Cuts at: 0 2521 2780 5046 Size: 2521 259 2266 Fragments arranged by size: 2521 2266 259 PstI C_TGCA'G Cuts at: 0 618 2112 2666 2721 2855 5046 Size: 618 1494 554 55 134 2191 Fragments arranged by size: 2191 1494 618 554 134 55 PvuII CAG'CTG Cuts at: 0 448 2880 4430 4962 4969 5046 Size: 448 2432 1550 532 7 77 Fragments arranged by size: 2432 1550 532 448 77 7 RsrII CG'GwC_CG Cuts at: 0 2782 5046 Size: 2782 2264 SacI G_AGCT'C Cuts at: 0 407 493 4226 5046 Size: 407 86 3733 820 Fragments arranged by size: 3733 820 407 86 SalI G'TCGA_C Cuts at: 0 4024 5046 Size: 4024 1022 SapI GCTCTTCn'nnn_ Cuts at: 0 320 455 1559 3335 3794 5046 Size: 320 135 1104 1776 459 1252 Fragments arranged by size: 1776 1252 1104 459 320 135 SbfI CC_TGCA'GG Cuts at: 0 2112 5046 Size: 2112 2934 ScaI AGT'ACT Cuts at: 0 1180 5046 Size: 1180 3866 SfcI C'TryA_G Cuts at: 0 614 1270 2108 2662 2705 2717 2851 5046 Size: 614 656 838 554 43 12 134 2195 Fragments arranged by size: 2195 838 656 614 554 134 43 12 SfoI GGC'GCC Cuts at: 0 1231 5046 Size: 1231 3815 SmaI CCC'GGG Cuts at: 0 3138 5046 Size: 3138 1908 SmlI C'TyrA_G Cuts at: 0 661 1123 2187 4980 5046 Size: 661 462 1064 2793 66 Fragments arranged by size: 2793 1064 661 462 66 SpeI A'CTAG_T Cuts at: 0 1738 5046 Size: 1738 3308 SphI G_CATG'C Cuts at: 0 897 2661 4581 5046 Size: 897 1764 1920 465 Fragments arranged by size: 1920 1764 897 465 SspI AAT'ATT Cuts at: 0 1459 3972 5046 Size: 1459 2513 1074 Fragments arranged by size: 2513 1459 1074 StuI AGG'CCT Cuts at: 0 1559 5046 Size: 1559 3487 StyI C'CwwG_G Cuts at: 0 1973 3929 4880 5046 Size: 1973 1956 951 166 Fragments arranged by size: 1973 1956 951 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1615 2305 2770 3029 3656 4639 5046 Size: 1615 690 465 259 627 983 407 Fragments arranged by size: 1615 983 690 627 465 407 259 TatI w'GTAC_w Cuts at: 0 1178 1658 4284 5046 Size: 1178 480 2626 762 Fragments arranged by size: 2626 1178 762 480 XbaI T'CTAG_A Cuts at: 0 4921 5046 Size: 4921 125 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 39 248 3105 4143 5046 Size: 39 209 2857 1038 903 Fragments arranged by size: 2857 1038 903 209 39 XmaI C'CCGG_G Cuts at: 0 3136 5046 Size: 3136 1910 XmnI GAAnn'nnTTC Cuts at: 0 1868 5029 5046 Size: 1868 3161 17 Fragments arranged by size: 3161 1868 17 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI AloI AlwNI ApaLI ApoI AvaI BanI BanII BbeI BbsI BbvCI BclI BfrBI BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PstI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI XbaI XcmI XmaI XmnI Enzymes that do not cut: AatII AfeI AflII AhdI AleI ApaI AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI BmgBI BmtI BsaBI BsiEI BsiWI BsmI BsrDI BssHII BstBI BstEII Bsu36I EagI FseI FspI FspAI HpaI MfeI MluI NheI NotI NruI PacI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI SacII SanDI SexAI SfiI SgfI SgrAI SnaBI SrfI SwaI TstI Tth111I XhoI ZraI