################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BbvCI BcgI BciVI BfaI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MluI MroI MseI MslI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccI 815 GTCTAC GT^[AC][GT]AC AlwI 668 GATCC GGATC(4/5) AviII 419 TGCGCA TGC^GCA BbsI 193 GTCTTC GAAGAC(2/6) BclI 959 TGATCA T^GATCA BglII 548 AGATCT A^GATCT BlpI 381 GCTGAGC GC^T[ACGT]AGC Bpu1102I 381 GCTGAGC GC^T[ACGT]AGC BpuAI 193 GTCTTC GAAGAC(2/6) BsaI 1312 GAGACC GGTCTC(1/5) BseRI 1191 CTCCTC GAGGAG(10/8) BsmBI 1303 GAGACG CGTCTC(1/5) BsmI 576 GAATGC GAATGC(1/-1) BspHI 848 TCATGA T^CATGA BstXI 1004 CCAGTGCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 548 AGATCT [GA]^GATC[CT] CelII 381 GCTGAGC GC^T[ACGT]AGC DraIII 216 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1252 GACGAGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 959 TGATCA T^GATCA FspI 419 TGCGCA TGC^GCA HgaI 1305 GACGC GACGC(5/10) MflI 548 AGATCT [GA]^GATC[CT] NcoI 1122 CCATGG C^CATGG PshAI 1128 GACCCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 848 TCATGA T^CATGA SapI 775 GCTCTTC GCTCTTC(1/4) SbfI 356 CCTGCAGG CCTGCA^GG SexAI 308 ACCAGGT A^CC[AT]GGT Sse8387I 356 CCTGCAGG CCTGCA^GG XhoII 548 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwNI 455 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG 949 CAGCACCTG ApaI 735 GGGCCC GGGCC^C 936 GGGCCC AvaII 1127 GGACC G^G[AT]CC 1260 GGTCC BbeI 3 GGCGCC GGCGC^C 1023 GGCGCC BsrFI 119 GCCGGC [GA]^CCGG[CT] 401 GCCGGC BssHII 377 GCGCGC G^CGCGC 410 GCGCGC Cfr10I 119 GCCGGC [GA]^CCGG[CT] 401 GCCGGC DsaI 524 CCACGG C^C[GA][CT]GG 1122 CCATGG EarI 776 CTCTTC CTCTTC(1/4) 1333 GAAGAG FauI 70 CCCGC CCCGC(4/6) 293 CCCGC KasI 3 GGCGCC G^GCGCC 1023 GGCGCC Ksp632I 776 CTCTTC CTCTTC(1/4) 1333 GAAGAG NaeI 119 GCCGGC GCC^GGC 401 GCCGGC NarI 3 GGCGCC GG^CGCC 1023 GGCGCC NgoMIV 119 GCCGGC G^CCGGC 401 GCCGGC NspI 115 ACATGC [GA]CATG^[CT] 183 ACATGC PleI 226 GACTC GAGTC(4/5) 1240 GAGTC PpuMI 1126 GGGACCC [GA]G^G[AT]CC[CT] 1259 AGGTCCC PvuII 749 CAGCTG CAG^CTG 1297 CAGCTG SfoI 3 GGCGCC GGC^GCC 1023 GGCGCC SmlI 279 CTTGAG C^T[CT][GA]AG 320 CTCAAG TfiI 1105 GATTC G^A[AT]TC 1225 GATTC XcmI 614 CCACCCGGAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1202 CCAGCTCCACAATGG ######################################### # 3 OR MORE CUT ENZYMES ( 86 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AspHI AvaI BalI BanI BanII BbvI BcnI BglI BmyI BpmI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmaI StyI TseI Tsp45I TspRI XmaCI XmaI