(Circular) (Six-base) MAPSORT of: pFN21AE1924.seq Check: 2187 from: 1 to: 6088 pFN21AE1924 6088 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2369 2967 3158 2369 Size: 598 191 5299 Fragments arranged by size: 5299 598 191 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2821 3431 1937 Size: 884 610 4594 Fragments arranged by size: 4594 884 610 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2778 4151 5535 5803 1632 Size: 1146 1373 1384 268 1917 Fragments arranged by size: 1917 1384 1373 1146 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 2465 2959 3831 5403 2465 Size: 494 872 1572 3150 Fragments arranged by size: 3150 1572 872 494 ApaI G_GGCC'C Cuts at: 2744 2945 2744 Size: 201 5887 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 3397 3623 3775 5884 1493 Size: 1904 226 152 2109 1697 Fragments arranged by size: 2109 1904 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 2111 2276 2337 2437 2500 2928 2945 Size: 73 168 165 61 100 63 428 17 Cuts at: 2945 3087 3413 1870 Size: 142 326 4545 Fragments arranged by size: 4545 428 326 168 165 142 100 73 63 61 17 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2008 2403 2902 3028 3052 Size: 325 217 627 221 395 499 126 24 Cuts at: 3052 3409 4864 618 Size: 357 1455 1842 Fragments arranged by size: 1842 1455 627 499 395 357 325 221 217 126 24 BanII G_rGCy'C Cuts at: 1391 2052 2112 2744 2945 2981 3005 3107 3407 Size: 661 60 632 201 36 24 102 300 Cuts at: 3407 1391 Size: 4072 Fragments arranged by size: 4072 661 632 300 201 102 60 36 24 BbeI G_GCGC'C Cuts at: 2012 3032 2012 Size: 1020 5068 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2191 928 Size: 971 292 4825 Fragments arranged by size: 4825 971 292 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4279 4313 1903 Size: 34 2342 34 3678 Fragments arranged by size: 3678 2342 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 2964 1548 Size: 1416 4672 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2024 2033 2084 Size: 107 122 71 631 605 352 9 51 Cuts at: 2084 2439 2639 2924 3074 3083 4699 136 Size: 355 200 285 150 9 1616 1525 Fragments arranged by size: 1616 1525 631 605 355 352 285 200 150 122 107 71 51 9 9 BglII A'GATC_T Cuts at: 2553 6083 2553 Size: 3530 2558 BlpI GC'TnA_GC Cuts at: 2387 3505 2387 Size: 1118 4970 Bme1580I G_kGCm'C Cuts at: 1790 2500 2744 2945 4149 5305 1790 Size: 710 244 201 1204 1156 2573 Fragments arranged by size: 2573 1204 1156 710 244 201 BmgBI CAC'GTC Cuts at: 1318 2479 2607 2613 1318 Size: 1161 128 6 4793 Fragments arranged by size: 4793 1161 128 6 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2278 3378 3819 Size: 416 53 83 399 24 861 1100 441 Cuts at: 3819 4777 442 Size: 958 1753 Fragments arranged by size: 1753 1100 958 861 441 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2679 2965 3017 3326 4748 1239 Size: 827 613 286 52 309 1422 2579 Fragments arranged by size: 2579 1422 827 613 309 286 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2305 2310 4213 5078 5376 2305 Size: 5 1903 865 298 3017 Fragments arranged by size: 3017 1903 865 298 5 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3311 4751 882 Size: 617 1812 1440 2219 Fragments arranged by size: 2219 1812 1440 617 BsaAI yAC'GTr Cuts at: 493 1204 3879 493 Size: 711 2675 2702 Fragments arranged by size: 2702 2675 711 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 3029 3311 3895 Size: 53 83 186 936 476 1020 282 584 Cuts at: 3895 4277 4912 275 Size: 382 635 1451 Fragments arranged by size: 1451 1020 936 635 584 476 382 282 186 83 53 BsaWI w'CCGG_w Cuts at: 4521 4919 5193 5340 5751 4521 Size: 398 274 147 411 4858 Fragments arranged by size: 4858 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5863 Fragments arranged by size: 5863 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3187 1186 Size: 2001 4087 BseYI C'CCAG_C Cuts at: 2256 2655 2702 2744 2752 3325 3371 5291 5787 Size: 399 47 42 8 573 46 1920 496 Cuts at: 5787 2256 Size: 2557 Fragments arranged by size: 2557 1920 573 496 399 47 46 42 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2317 2341 2587 1163 Size: 573 581 24 246 4664 Fragments arranged by size: 4664 581 573 246 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3538 4299 5327 1540 Size: 275 186 1537 761 1028 2301 Fragments arranged by size: 2301 1537 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2052 2120 2768 2981 3005 3107 3197 3335 4149 Size: 68 648 213 24 102 90 138 814 Cuts at: 4149 4234 5305 2052 Size: 85 1071 2835 Fragments arranged by size: 2835 1071 814 648 213 138 102 90 85 68 24 BsmI GAATG_Cn' Cuts at: 2587 3599 3692 2587 Size: 1012 93 4983 Fragments arranged by size: 4983 1012 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3302 3302 Size: 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2112 2120 2500 2744 2768 2945 Size: 399 262 60 8 380 244 24 177 Cuts at: 2945 2981 3005 3107 3197 3335 3407 4149 4234 Size: 36 24 102 90 138 72 742 85 Cuts at: 4234 5305 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 262 244 177 138 102 90 85 72 60 36 24 24 8 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 2853 3977 5707 2853 Size: 1124 1730 3234 Fragments arranged by size: 3234 1730 1124 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2369 2967 3158 3253 3443 844 Size: 1525 598 191 95 190 3489 Fragments arranged by size: 3489 1525 598 191 190 95 BsrBI CCG'CTC Cuts at: 1394 1823 3975 1394 Size: 429 2152 3507 Fragments arranged by size: 3507 2152 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2124 2406 4732 4919 1700 Size: 110 314 282 2326 187 2869 Fragments arranged by size: 2869 2326 314 282 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 2382 2415 1925 Size: 457 33 5598 Fragments arranged by size: 5598 457 33 BssSI C'ACGA_G Cuts at: 4148 5160 4148 Size: 1012 5076 BstAPI GCAn_nnn'nTGC Cuts at: 794 2071 2172 2959 794 Size: 1277 101 787 3923 Fragments arranged by size: 3923 1277 787 101 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstXI CCAn_nnnn'nTGG Cuts at: 3016 3016 Size: 6088 BstYI r'GATC_y Cuts at: 1972 2553 3418 3785 4170 4187 5639 5725 5737 Size: 581 865 367 385 17 1452 86 12 Cuts at: 5737 6083 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 865 581 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2529 3127 513 Size: 552 939 525 598 3474 Fragments arranged by size: 3474 939 598 552 525 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 2706 3604 4398 4418 2706 Size: 898 794 20 4376 Fragments arranged by size: 4376 898 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DraIII CAC_nnn'GTG Cuts at: 2226 2226 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3263 5095 809 Size: 507 1947 1832 1802 Fragments arranged by size: 1947 1832 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2451 2568 Size: 54 1425 50 275 18 204 417 117 Cuts at: 2568 3006 3124 3535 4424 6053 8 Size: 438 118 411 889 1629 43 Fragments arranged by size: 1629 1425 889 438 417 411 275 204 118 117 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3535 1537 Size: 275 1723 4090 Fragments arranged by size: 4090 1723 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2787 3333 4018 1353 Size: 1434 546 685 3423 Fragments arranged by size: 3423 1434 685 546 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2050 2979 3003 3105 2050 Size: 929 24 102 5033 Fragments arranged by size: 5033 929 102 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2679 2778 2965 3017 3326 4151 Size: 393 434 613 99 187 52 309 825 Cuts at: 4151 4748 5535 5803 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 825 787 613 597 434 393 309 268 187 99 52 EcoO109I rG'GnC_Cy Cuts at: 2544 2741 2941 2942 3132 3265 2544 Size: 197 200 1 190 133 5367 Fragments arranged by size: 5367 200 197 190 133 1 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2893 2893 Size: 6088 FspI TGC'GCA Cuts at: 2426 4594 2426 Size: 2168 3920 HaeII r_GCGC'y Cuts at: 1678 2012 2975 3032 5235 1678 Size: 334 963 57 2203 2531 Fragments arranged by size: 2531 2203 963 334 57 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2034 2066 3170 3202 4729 Size: 32 135 32 462 32 1104 32 1527 Cuts at: 4729 4761 4803 4835 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1527 1104 462 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3432 3678 669 Size: 988 281 1494 246 3079 Fragments arranged by size: 3079 1494 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KasI G'GCGC_C Cuts at: 2008 3028 2008 Size: 1020 5068 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2020 2538 2770 2997 5202 5386 1305 Size: 715 518 232 227 2205 184 2007 Fragments arranged by size: 2205 2007 715 518 232 227 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2453 2570 3126 10 Size: 54 1425 343 621 117 556 2972 Fragments arranged by size: 2972 1425 621 556 343 117 54 MslI CAynn'nnrTG Cuts at: 518 1166 4046 4405 4564 518 Size: 648 2880 359 159 2042 Fragments arranged by size: 2880 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2301 2422 2756 3304 3504 4181 5329 5574 Size: 695 121 334 548 200 677 1148 245 Cuts at: 5574 1606 Size: 2120 Fragments arranged by size: 2120 1148 695 677 548 334 245 200 121 NaeI GCC'GGC Cuts at: 2126 2408 2126 Size: 282 5806 NarI GG'CG_CC Cuts at: 2009 3029 2009 Size: 1020 5068 NcoI C'CATG_G Cuts at: 513 1065 2004 3127 513 Size: 552 939 1123 3474 Fragments arranged by size: 3474 1123 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NgoMIV G'CCGG_C Cuts at: 2124 2406 2124 Size: 282 5806 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2124 2192 3446 4991 1132 Size: 97 895 68 1254 1545 2229 Fragments arranged by size: 2229 1545 1254 895 97 68 PasI CC'CwG_GG Cuts at: 1348 2576 2636 1348 Size: 1228 60 4800 Fragments arranged by size: 4800 1228 60 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6088 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3132 3265 3132 Size: 133 5955 PshAI GACnn'nnGTC Cuts at: 1613 3137 1613 Size: 1524 4564 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PspOMI G'GGCC_C Cuts at: 2740 2941 2740 Size: 201 5887 PstI C_TGCA'G Cuts at: 830 2168 2366 2889 3440 830 Size: 1338 198 523 551 3478 Fragments arranged by size: 3478 1338 551 523 198 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 PvuII CAG'CTG Cuts at: 2756 3304 2756 Size: 548 5540 SacI G_AGCT'C Cuts at: 2052 2981 3005 3107 2052 Size: 929 24 102 5033 Fragments arranged by size: 5033 929 102 24 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SanDI GG'GwC_CC Cuts at: 3132 3132 Size: 6088 SapI GCTCTTCn'nnn_ Cuts at: 2787 2787 Size: 6088 SbfI CC_TGCA'GG Cuts at: 2366 3440 2366 Size: 1074 5014 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SexAI A'CCwGG_T Cuts at: 2313 2313 Size: 6088 SfcI C'TryA_G Cuts at: 826 1045 2164 2302 2362 2885 3166 3436 4571 Size: 219 1119 138 60 523 281 270 1135 Cuts at: 4571 5252 5443 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1119 681 523 281 270 219 191 138 60 SfiI GGCCn_nnn'nGGCC Cuts at: 2033 3074 3083 2033 Size: 1041 9 5038 Fragments arranged by size: 5038 1041 9 SfoI GGC'GCC Cuts at: 2010 3030 2010 Size: 1020 5068 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 2113 2439 2502 2930 3089 3415 1872 Size: 241 326 63 428 159 326 4545 Fragments arranged by size: 4545 428 326 326 241 159 63 SmlI C'TyrA_G Cuts at: 820 1017 1943 2284 2325 4192 5093 5355 820 Size: 197 926 341 41 1867 901 262 1553 Fragments arranged by size: 1867 1553 926 901 341 262 197 41 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SrfI GCCC'GGGC Cuts at: 2439 3089 2439 Size: 650 5438 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2004 2814 2898 3127 3276 3355 3527 Size: 552 939 810 84 229 149 79 172 Cuts at: 3527 513 Size: 3074 Fragments arranged by size: 3074 939 810 552 229 172 149 84 79 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2270 2792 3285 4115 4132 4285 4470 Size: 1255 690 522 493 830 17 153 185 Cuts at: 4470 4915 325 Size: 445 1498 Fragments arranged by size: 1498 1255 830 690 522 493 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2644 4334 6046 Size: 274 80 33 51 1443 667 1690 1712 Cuts at: 6046 96 Size: 138 Fragments arranged by size: 1712 1690 1443 667 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2210 2242 2784 2816 6048 6080 2210 Size: 32 542 32 3232 32 2218 Fragments arranged by size: 3232 2218 542 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2626 3214 2626 Size: 588 5500 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 2111 2437 2500 2928 3087 3413 1870 Size: 241 326 63 428 159 326 4545 Fragments arranged by size: 4545 428 326 326 241 159 63 XmnI GAAnn'nnTTC Cuts at: 4217 4217 Size: 6088 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SwaI