(Linear) (Six-base) MAPSORT of: KIEE1924.seq Check: 4248 from: 1 to: 1383 KIEE1924.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 364 962 1153 1383 Size: 364 598 191 230 Fragments arranged by size: 598 364 230 191 AccI GT'mk_AC Cuts at: 0 816 1383 Size: 816 567 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 773 1383 Size: 773 610 AlwNI CAG_nnn'CTG Cuts at: 0 460 954 1383 Size: 460 494 429 Fragments arranged by size: 494 460 429 ApaI G_GGCC'C Cuts at: 0 739 940 1383 Size: 739 201 443 Fragments arranged by size: 739 443 201 AvaI C'yCGr_G Cuts at: 0 106 271 332 432 495 923 940 1082 Size: 106 165 61 100 63 428 17 142 Cuts at: 1082 1383 Size: 301 Fragments arranged by size: 428 301 165 142 106 100 63 61 17 BanI G'GyrC_C Cuts at: 0 3 398 897 1023 1047 1383 Size: 3 395 499 126 24 336 Fragments arranged by size: 499 395 336 126 24 3 BanII G_rGCy'C Cuts at: 0 47 107 739 940 976 1000 1102 1383 Size: 47 60 632 201 36 24 102 281 Fragments arranged by size: 632 281 201 102 60 47 36 24 BbeI G_GCGC'C Cuts at: 0 7 1027 1383 Size: 7 1020 356 Fragments arranged by size: 1020 356 7 BbsI GAAGACnn'nnnn_ Cuts at: 0 186 1383 Size: 186 1197 BclI T'GATC_A Cuts at: 0 959 1383 Size: 959 424 BglI GCCn_nnn'nGGC Cuts at: 0 19 28 79 434 634 919 1069 1078 Size: 19 9 51 355 200 285 150 9 Cuts at: 1078 1383 Size: 305 Fragments arranged by size: 355 305 285 200 150 51 19 9 9 BglII A'GATC_T Cuts at: 0 548 1383 Size: 548 835 BlpI GC'TnA_GC Cuts at: 0 382 1383 Size: 382 1001 Bme1580I G_kGCm'C Cuts at: 0 495 739 940 1383 Size: 495 244 201 443 Fragments arranged by size: 495 443 244 201 BmgBI CAC'GTC Cuts at: 0 474 602 608 1383 Size: 474 128 6 775 Fragments arranged by size: 775 474 128 6 BmrI ACTGGGnnnn_n' Cuts at: 0 273 1373 1383 Size: 273 1100 10 Fragments arranged by size: 1100 273 10 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 674 960 1012 1321 1383 Size: 61 613 286 52 309 62 Fragments arranged by size: 613 309 286 62 61 52 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 300 305 1383 Size: 300 5 1078 Fragments arranged by size: 1078 300 5 BsaI GGTCTCn'nnnn_ Cuts at: 0 1306 1383 Size: 1306 77 BsaHI Gr'CG_yC Cuts at: 0 4 1024 1306 1383 Size: 4 1020 282 77 Fragments arranged by size: 1020 282 77 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1182 1383 Size: 1182 201 BseYI C'CCAG_C Cuts at: 0 251 650 697 739 747 1320 1366 1383 Size: 251 399 47 42 8 573 46 17 Fragments arranged by size: 573 399 251 47 46 42 17 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 312 336 582 1383 Size: 312 24 246 801 Fragments arranged by size: 801 312 246 24 BsiHKAI G_wGCw'C Cuts at: 0 47 115 763 976 1000 1102 1192 1330 Size: 47 68 648 213 24 102 90 138 Cuts at: 1330 1383 Size: 53 Fragments arranged by size: 648 213 138 102 90 68 53 47 24 BsmI GAATG_Cn' Cuts at: 0 582 1383 Size: 582 801 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1297 1383 Size: 1297 86 Bsp1286I G_dGCh'C Cuts at: 0 47 107 115 495 739 763 940 976 Size: 47 60 8 380 244 24 177 36 Cuts at: 976 1000 1102 1192 1330 1383 Size: 24 102 90 138 53 Fragments arranged by size: 380 244 177 138 102 90 60 53 47 36 24 24 8 BspHI T'CATG_A Cuts at: 0 848 1383 Size: 848 535 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 364 962 1153 1248 1383 Size: 364 598 191 95 135 Fragments arranged by size: 598 364 191 135 95 BsrFI r'CCGG_y Cuts at: 0 119 401 1383 Size: 119 282 982 Fragments arranged by size: 982 282 119 BssHII G'CGCG_C Cuts at: 0 377 410 1383 Size: 377 33 973 Fragments arranged by size: 973 377 33 BstAPI GCAn_nnn'nTGC Cuts at: 0 66 167 954 1383 Size: 66 101 787 429 Fragments arranged by size: 787 429 101 66 BstXI CCAn_nnnn'nTGG Cuts at: 0 1011 1383 Size: 1011 372 BstYI r'GATC_y Cuts at: 0 548 1383 Size: 548 835 BtgI C'CryG_G Cuts at: 0 524 1122 1383 Size: 524 598 261 Fragments arranged by size: 598 524 261 BtsI GCAGTG_nn' Cuts at: 0 701 1383 Size: 701 682 DraIII CAC_nnn'GTG Cuts at: 0 221 1383 Size: 221 1162 DrdI GACnn_nn'nnGTC Cuts at: 0 1258 1383 Size: 1258 125 EaeI y'GGCC_r Cuts at: 0 29 446 563 1001 1119 1383 Size: 29 417 117 438 118 264 Fragments arranged by size: 438 417 264 118 117 29 EarI CTCTTCn'nnn_ Cuts at: 0 782 1328 1383 Size: 782 546 55 Fragments arranged by size: 782 546 55 EcoICRI GAG'CTC Cuts at: 0 45 974 998 1100 1383 Size: 45 929 24 102 283 Fragments arranged by size: 929 283 102 45 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 61 674 773 960 1012 1321 1383 Size: 61 613 99 187 52 309 62 Fragments arranged by size: 613 309 187 99 62 61 52 EcoO109I rG'GnC_Cy Cuts at: 0 539 736 936 937 1127 1260 1383 Size: 539 197 200 1 190 133 123 Fragments arranged by size: 539 200 197 190 133 123 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 888 1383 Size: 888 495 FspI TGC'GCA Cuts at: 0 421 1383 Size: 421 962 HaeII r_GCGC'y Cuts at: 0 7 970 1027 1383 Size: 7 963 57 356 Fragments arranged by size: 963 356 57 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 29 61 1165 1197 1383 Size: 29 32 1104 32 186 Fragments arranged by size: 1104 186 32 32 29 KasI G'GCGC_C Cuts at: 0 3 1023 1383 Size: 3 1020 360 Fragments arranged by size: 1020 360 3 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 15 533 765 992 1383 Size: 15 518 232 227 391 Fragments arranged by size: 518 391 232 227 15 MscI TGG'CCA Cuts at: 0 448 565 1121 1383 Size: 448 117 556 262 Fragments arranged by size: 556 448 262 117 MspA1I CmG'CkG Cuts at: 0 296 417 751 1299 1383 Size: 296 121 334 548 84 Fragments arranged by size: 548 334 296 121 84 NaeI GCC'GGC Cuts at: 0 121 403 1383 Size: 121 282 980 Fragments arranged by size: 980 282 121 NarI GG'CG_CC Cuts at: 0 4 1024 1383 Size: 4 1020 359 Fragments arranged by size: 1020 359 4 NcoI C'CATG_G Cuts at: 0 1122 1383 Size: 1122 261 NgoMIV G'CCGG_C Cuts at: 0 119 401 1383 Size: 119 282 982 Fragments arranged by size: 982 282 119 NspI r_CATG'y Cuts at: 0 119 187 1383 Size: 119 68 1196 Fragments arranged by size: 1196 119 68 PasI CC'CwG_GG Cuts at: 0 571 631 1383 Size: 571 60 752 Fragments arranged by size: 752 571 60 PpuMI rG'GwC_Cy Cuts at: 0 1127 1260 1383 Size: 1127 133 123 Fragments arranged by size: 1127 133 123 PshAI GACnn'nnGTC Cuts at: 0 1132 1383 Size: 1132 251 PspOMI G'GGCC_C Cuts at: 0 735 936 1383 Size: 735 201 447 Fragments arranged by size: 735 447 201 PstI C_TGCA'G Cuts at: 0 163 361 884 1383 Size: 163 198 523 499 Fragments arranged by size: 523 499 198 163 PvuII CAG'CTG Cuts at: 0 751 1299 1383 Size: 751 548 84 Fragments arranged by size: 751 548 84 SacI G_AGCT'C Cuts at: 0 47 976 1000 1102 1383 Size: 47 929 24 102 281 Fragments arranged by size: 929 281 102 47 24 SanDI GG'GwC_CC Cuts at: 0 1127 1383 Size: 1127 256 SapI GCTCTTCn'nnn_ Cuts at: 0 782 1383 Size: 782 601 SbfI CC_TGCA'GG Cuts at: 0 361 1383 Size: 361 1022 SexAI A'CCwGG_T Cuts at: 0 308 1383 Size: 308 1075 SfcI C'TryA_G Cuts at: 0 159 297 357 880 1161 1383 Size: 159 138 60 523 281 222 Fragments arranged by size: 523 281 222 159 138 60 SfiI GGCCn_nnn'nGGCC Cuts at: 0 28 1069 1078 1383 Size: 28 1041 9 305 Fragments arranged by size: 1041 305 28 9 SfoI GGC'GCC Cuts at: 0 5 1025 1383 Size: 5 1020 358 Fragments arranged by size: 1020 358 5 SmaI CCC'GGG Cuts at: 0 108 434 497 925 1084 1383 Size: 108 326 63 428 159 299 Fragments arranged by size: 428 326 299 159 108 63 SmlI C'TyrA_G Cuts at: 0 279 320 1383 Size: 279 41 1063 Fragments arranged by size: 1063 279 41 SrfI GCCC'GGGC Cuts at: 0 434 1084 1383 Size: 434 650 299 Fragments arranged by size: 650 434 299 StyI C'CwwG_G Cuts at: 0 809 893 1122 1271 1350 1383 Size: 809 84 229 149 79 33 Fragments arranged by size: 809 229 149 84 79 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 265 787 1280 1383 Size: 265 522 493 103 Fragments arranged by size: 522 493 265 103 TatI w'GTAC_w Cuts at: 0 639 1383 Size: 639 744 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 205 237 779 811 1383 Size: 205 32 542 32 572 Fragments arranged by size: 572 542 205 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 621 1209 1383 Size: 621 588 174 Fragments arranged by size: 621 588 174 XmaI C'CCGG_G Cuts at: 0 106 432 495 923 1082 1383 Size: 106 326 63 428 159 301 Fragments arranged by size: 428 326 301 159 106 63 Enzymes that do cut: AarI AccI AcuI AlwNI ApaI AvaI BanI BanII BbeI BbsI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaHI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrFI BssHII BstAPI BstXI BstYI BtgI BtsI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I FalI FspI HaeII Hin4I KasI MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI SrfI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BmtI BplI Bpu10I BsaAI BsaBI BsaWI BsaXI BsiEI BsiWI BspEI BsrBI BsrDI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI EagI EciI EcoNI EcoRI EcoRV FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MslI NdeI NheI NotI NruI NsiI PacI PciI PflMI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI