Restriction map for
pFN21AE5161
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# NO CUT ENZYMES ( 183 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AviII AvrII
BalI BbeI BbrPI BbsI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI Hin1I HincII HindII
HindIII HinfI HpaI HphI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tru9I
Tsp45I Tsp509I Tth111I TthHB8I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 26 enzymes) #
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BamHI 3 GGATCC G^GATCC
BbvCI 390 GCTGAGG CCTCAGC(-5/-2)
BsaWI 353 ACCGGA [AT]^CCGG[AT]
BseRI 363 CTCCTC GAGGAG(10/8)
BspMI 171 GCAGGT ACCTGC(4/8)
BsrI 368 CCAGT ACTGG(1/-1)
BstXI 368 CCAGTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3 GGATCC [GA]^GATC[CT]
Cac8I 294 GCGGGC GC[ACGT]^[ACGT]GC
DdeI 391 CTGAG C^T[ACGT]AG
DsaI 261 CCGTGG C^C[GA][CT]GG
EarI 43 CTCTTC CTCTTC(1/4)
HaeII 27 GGCGCT [GA]GCGC^[CT]
HaeIII 373 GGCC GG^CC
Ksp632I 43 CTCTTC CTCTTC(1/4)
MaeII 118 ACGT A^CGT
MaeIII 126 GTTAC ^GT[ACGT]AC
MflI 3 GGATCC [GA]^GATC[CT]
MspA1I 82 CCGCTG C[AC]G^C[GT]G
PflMI 78 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 169 CTGCAG CTGCA^G
SapI 42 GCTCTTC GCTCTTC(1/4)
SfcI 169 CTGCAG C^T[GA][CT]AG
TspRI 369 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 78 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 3 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AluI 167 AGCT AG^CT
361 AGCT
AvaI 23 CCCGGG C^[CT]CG[GA]G
236 CCCGGG
AvaII 279 GGTCC G^G[AT]CC
351 GGACC
BanI 266 GGCACC G^G[CT][GA]CC
297 GGCACC
BanII 193 GAGCCC G[GA]GC[CT]^C
207 GAGCCC
BsmFI 215 GTCCC GGGAC(10/14)
234 GTCCC
BsoBI 23 CCCGGG C^[CT]CG[GA]G
236 CCCGGG
BstF5I 185 CATCC GGATG(2/0)
258 CATCC
CfoI 28 GCGC GCG^C
138 GCGC
Cfr13I 279 GGTCC G^G[ACGT]CC
351 GGACC
DpnII 4 GATC ^GATC
357 GATC
FauI 81 CCCGC CCCGC(4/6)
294 GCGGG
FokI 185 CATCC GGATG(9/13)
258 CATCC
HhaI 28 GCGC GCG^C
138 GCGC
HinP1I 28 GCGC G^CGC
138 GCGC
MboI 4 GATC ^GATC
357 GATC
MboII 345 GAAGA GAAGA(8/7)
44 TCTTC
NdeII 4 GATC ^GATC
357 GATC
Sau3AI 4 GATC ^GATC
357 GATC
Sau96I 279 GGTCC G^G[ACGT]CC
351 GGACC
SfaNI 51 GCATC GCATC(5/9)
257 GCATC
SmaI 23 CCCGGG CCC^GGG
236 CCCGGG
XmaCI 23 CCCGGG C^CCGGG
236 CCCGGG
XmaI 23 CCCGGG C^CCGGG
236 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AciI AlwI BbvI BcnI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BssKI BstNI EcoRII Fnu4HI HapII
HpaII ItaI MnlI MspI MvaI
MwoI NciI NlaIV ScrFI TseI