################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaI BseAI BseRI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI MaeI MamI MfeI MluI MluNI MroI MscI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SalI SapI ScaI SexAI SfiI SfuI SmlI SnaBI SpeI SphI SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 766 CGCG CG^CG AcsI 955 AAATTC [GA]^AATT[CT] AocI 154 CCTCAGG CC^T[ACGT]AGG Aor51HI 141 AGCGCT AGC^GCT ApoI 955 AAATTC [GA]^AATT[CT] BamHI 1030 GGATCC G^GATCC BbsI 715 GAAGAC GAAGAC(2/6) BbvCI 277 GCTGAGG CCTCAGC(-5/-2) BlpI 63 GCTGAGC GC^T[ACGT]AGC Bpu1102I 63 GCTGAGC GC^T[ACGT]AGC BpuAI 715 GAAGAC GAAGAC(2/6) BsaWI 962 ACCGGA [AT]^CCGG[AT] BsrBI 23 CCGCTC CCGCTC(-3/-3) BsrDI 347 CATTGC GCAATG(2/0) BsrFI 173 GCCGGC [GA]^CCGG[CT] BstAPI 586 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 240 GGTCACC G^GT[ACGT]ACC BstPI 240 GGTCACC G^GT[ACGT]ACC BstUI 766 CGCG CG^CG BstYI 1030 GGATCC [GA]^GATC[CT] Bsu36I 154 CCTCAGG CC^T[ACGT]AGG CelII 63 GCTGAGC GC^T[ACGT]AGC Cfr10I 173 GCCGGC [GA]^CCGG[CT] EaeI 1013 CGGCCA [CT]^GGCC[GA] EarI 601 GAAGAG CTCTTC(1/4) Eco47III 141 AGCGCT AGC^GCT Eco81I 154 CCTCAGG CC^T[ACGT]AGG EcoNI 312 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 240 GGTCACC G^GT[ACGT]ACC Ksp632I 601 GAAGAG CTCTTC(1/4) KspI 765 CCGCGG CCGC^GG MaeII 1043 ACGT A^CGT MflI 1030 GGATCC [GA]^GATC[CT] MseI 721 TTAA T^TAA MslI 924 CATGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 765 CCGCGG C[AC]G^C[GT]G MvnI 766 CGCG CG^CG NaeI 173 GCCGGC GCC^GGC NcoI 527 CCATGG C^CATGG NgoMIV 173 GCCGGC G^CCGGC NspI 923 GCATGT [GA]CATG^[CT] PflMI 510 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 496 GAGCTC GAGCT^C SacII 765 CCGCGG CCGC^GG SbfI 304 CCTGCAGG CCTGCA^GG SgrAI 172 CGCCGGCG C[GA]^CCGG[CT]G SmaI 183 CCCGGG CCC^GGG Sse8387I 304 CCTGCAGG CCTGCA^GG Tru9I 721 TTAA T^TAA Tsp509I 956 AATT ^AATT TspRI 780 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 510 CCAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1030 GGATCC [GA]^GATC[CT] XmaCI 183 CCCGGG C^CCGGG XmaI 183 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### ApaI 180 GGGCCC GGGCC^C 201 GGGCCC AspHI 412 GAGCAC G[AT]GC[AT]^C 496 GAGCTC BanI 76 GGCGCC G^G[CT][GA]CC 552 GGCGCC BbeI 76 GGCGCC GGCGC^C 552 GGCGCC BglI 68 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 173 GCCGGCGGGGC BsgI 442 CTGCAC GTGCAG(16/14) 876 CTGCAC BsiHKAI 412 GAGCAC G[AT]GC[AT]^C 496 GAGCTC BsmAI 420 GAGAC GTCTC(1/5) 1009 GAGAC BsmBI 420 GAGACG CGTCTC(1/5) 1009 GAGACG BstXI 139 CCAGCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 542 CCACCAGCCTGG DpnII 612 GATC ^GATC 1031 GATC DsaI 527 CCATGG C^C[GA][CT]GG 765 CCGCGG FauI 177 GCGGG CCCGC(4/6) 864 GCGGG HgaI 150 GACGC GACGC(5/10) 422 GACGC KasI 76 GGCGCC G^GCGCC 552 GGCGCC MboI 612 GATC ^GATC 1031 GATC NarI 76 GGCGCC GG^CGCC 552 GGCGCC NdeII 612 GATC ^GATC 1031 GATC PleI 300 GAGTC GAGTC(4/5) 604 GAGTC PstI 305 CTGCAG CTGCA^G 316 CTGCAG Sau3AI 612 GATC ^GATC 1031 GATC SfcI 305 CTGCAG C^T[GA][CT]AG 316 CTGCAG SfoI 76 GGCGCC GGC^GCC 552 GGCGCC TaqI 698 TCGA T^CGA 914 TCGA TfiI 117 GAATC G^A[AT]TC 535 GAATC Tsp45I 241 GTCAC ^GT[GC]AC 622 GTCAC TthHB8I 698 TCGA T^CGA 914 TCGA XcmI 219 CCAGGCCCAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 677 CCACGCTGGACATGG ######################################### # 3 OR MORE CUT ENZYMES ( 60 enzymes) # ######################################### AciI AcyI AluI AlwI AlwNI AvaI AvaII BanII BbvI BcnI BmyI BpmI BsaHI BsaJI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeIII MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV PpuMI Sau96I ScrFI SfaNI StuI StyI TseI