Restriction map for
pFN21AE2608
(Circular) (Six-base) MAPSORT of: pFN21AE2608.seq Check: 3142 from: 1 to: 5752
pFN21AE2608 5752 bp
With 182 enzymes: SgfI *
February 23, 2016 18:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2605 2605
Size: 5752
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3815 5199 5467 1632
Size: 2183 1384 268 1917
Fragments arranged by size:
2183 1917 1384 268
AfeI AGC'GCT
Cuts at: 2148 2148
Size: 5752
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 4651 5628 1203
Size: 3448 977 1327
Fragments arranged by size:
3448 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2325 1184
Size: 1141 4611
AlwNI CAG_nnn'CTG
Cuts at: 2282 2521 2597 3495 5067 2282
Size: 239 76 898 1572 2967
Fragments arranged by size:
2967 1572 898 239 76
ApaI G_GGCC'C
Cuts at: 2189 2210 2189
Size: 21 5731
ApaLI G'TGCA_C
Cuts at: 3809 4965 3809
Size: 1156 4596
ApoI r'AATT_y
Cuts at: 1493 2960 3061 3287 3439 5548 1493
Size: 1467 101 226 152 2109 1697
Fragments arranged by size:
2109 1697 1467 226 152 101
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2188 2363 3011 3077 1870
Size: 73 245 175 648 66 4545
Fragments arranged by size:
4545 648 245 175 73 66
BamHI G'GATC_C
Cuts at: 3035 3082 3449 3035
Size: 47 367 5338
Fragments arranged by size:
5338 367 47
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2081 2557 3073 4528 618
Size: 325 217 627 294 476 516 1455 1842
Fragments arranged by size:
1842 1455 627 516 476 325 294 217
BanII G_rGCy'C
Cuts at: 1391 2189 2210 2505 3012 3071 1391
Size: 798 21 295 507 59 4072
Fragments arranged by size:
4072 798 507 295 59 21
BbeI G_GCGC'C
Cuts at: 2085 2561 2085
Size: 476 5276
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2727 928
Size: 971 828 3953
Fragments arranged by size:
3953 971 828
BbvCI CC'TCA_GC
Cuts at: 2283 2283
Size: 5752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2079 2184 4363
Size: 107 122 71 631 605 407 105 2179
Cuts at: 4363 136
Size: 1525
Fragments arranged by size:
2179 1525 631 605 407 122 107 105
71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 2069 3169 2069
Size: 1100 4652
Bme1580I G_kGCm'C
Cuts at: 1790 2189 2210 3813 4969 1790
Size: 399 21 1603 1156 2573
Fragments arranged by size:
2573 1603 1156 399 21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2560 2560
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2084 2251 2367 2767 4412 1239
Size: 845 167 116 400 1645 2579
Fragments arranged by size:
2579 1645 845 400 167 116
Bpu10I CC'TnA_GC
Cuts at: 2283 2298 2283
Size: 15 5737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3543 493
Size: 711 2339 2702
Fragments arranged by size:
2702 2339 711
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2082 2156 2558 3559
Size: 53 83 186 936 549 74 402 1001
Cuts at: 3559 3941 4576 275
Size: 382 635 1451
Fragments arranged by size:
1451 1001 936 635 549 402 382 186
83 74 53
BsaWI w'CCGG_w
Cuts at: 2967 4185 4583 4857 5004 5415 2967
Size: 1218 398 274 147 411 3304
Fragments arranged by size:
3304 1218 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5527
Fragments arranged by size:
5527 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5752
BseYI C'CCAG_C
Cuts at: 2009 2143 2219 2477 2643 4955 5451 2009
Size: 134 76 258 166 2312 496 2310
Fragments arranged by size:
2312 2310 496 258 166 134 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2432 2866 1163
Size: 573 696 434 4049
Fragments arranged by size:
4049 696 573 434
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2421 2505 3813 3898 4969 2421
Size: 84 1308 85 1071 3204
Fragments arranged by size:
3204 1308 1071 85 84
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
BsmBI CGTCTCn'nnnn_
Cuts at: 2419 3008 2419
Size: 589 5163
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2189 2210 2421 2505 3012 3071 3813
Size: 399 399 21 211 84 507 59 742
Cuts at: 3813 3898 4969 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 507 399 399 211 85
84 59 21
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2343 2605 2874 3107 844
Size: 1499 262 269 233 3489
Fragments arranged by size:
3489 1499 269 262 233
BsrBI CCG'CTC
Cuts at: 1394 1823 2030 3639 1394
Size: 429 207 1609 3507
Fragments arranged by size:
3507 1609 429 207
BsrDI GCAATG_nn'
Cuts at: 65 1248 2351 4247 4421 65
Size: 1183 1103 1896 174 1396
Fragments arranged by size:
1896 1396 1183 1103 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2178 4396 4583 1700
Size: 110 368 2218 187 2869
Fragments arranged by size:
2869 2218 368 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2597 794
Size: 1803 3949
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 2245 1184
Size: 1061 4691
BstXI CCAn_nnnn'nTGG
Cuts at: 2151 2554 2151
Size: 403 5349
BstYI r'GATC_y
Cuts at: 1972 3035 3082 3449 3834 3851 5303 5389 5401
Size: 1063 47 367 385 17 1452 86 12
Cuts at: 5401 5747 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1063 385 367 346 86 47
17 12
Bsu36I CC'TnA_GG
Cuts at: 2160 2160
Size: 5752
BtgI C'CryG_G
Cuts at: 513 1065 2004 2532 2770 513
Size: 552 939 528 238 3495
Fragments arranged by size:
3495 939 552 528 238
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 3268 4062 4082 3268
Size: 794 20 4938
Fragments arranged by size:
4938 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3018 3199 4088
Size: 54 1425 50 275 18 1188 181 889
Cuts at: 4088 5717 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1188 889 275 181 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2601 3682 1353
Size: 1248 1081 3423
Fragments arranged by size:
3423 1248 1081
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2503 2503
Size: 5752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2084 2251 2367 2767 3815 4412 5199
Size: 393 452 167 116 400 1048 597 787
Cuts at: 5199 5467 1239
Size: 268 1524
Fragments arranged by size:
1524 1048 787 597 452 400 393 268
167 116
EcoNI CCTnn'n_nnAGG
Cuts at: 2321 2321
Size: 5752
EcoO109I rG'GnC_Cy
Cuts at: 2097 2185 2206 2332 2474 2097
Size: 88 21 126 142 5375
Fragments arranged by size:
5375 142 126 88 21
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2085 2150 2561 3001 4899 1678
Size: 407 65 411 440 1898 2531
Fragments arranged by size:
2531 1898 440 411 407 65
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2451 2483 3020 3027 3052
Size: 32 135 32 879 32 537 7 25
Cuts at: 3052 3059 4393 4425 4467 4499 1373
Size: 7 1334 32 42 32 2626
Fragments arranged by size:
2626 1334 879 537 135 42 32 32
32 32 32 25 7 7
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KasI G'GCGC_C
Cuts at: 2081 2557 2081
Size: 476 5276
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2933 3710 4069 4228 518
Size: 648 1767 777 359 159 2042
Fragments arranged by size:
2042 1767 777 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2772 3168 3845 4993 5238 1606
Size: 1166 396 677 1148 245 2120
Fragments arranged by size:
2120 1166 1148 677 396 245
NaeI GCC'GGC
Cuts at: 2180 2180
Size: 5752
NarI GG'CG_CC
Cuts at: 2082 2558 2082
Size: 476 5276
NcoI C'CATG_G
Cuts at: 513 1065 2004 2532 513
Size: 552 939 528 3733
Fragments arranged by size:
3733 939 552 528
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NgoMIV G'CCGG_C
Cuts at: 2178 2178
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 2932 3110 4655 1132
Size: 97 1703 178 1545 2229
Fragments arranged by size:
2229 1703 1545 178 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2521 1108
Size: 1413 4339
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2097 2332 2474 2097
Size: 235 142 5375
Fragments arranged by size:
5375 235 142
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PspOMI G'GGCC_C
Cuts at: 2185 2206 2185
Size: 21 5731
PstI C_TGCA'G
Cuts at: 830 2314 2325 3104 830
Size: 1484 11 779 3478
Fragments arranged by size:
3478 1484 779 11
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
SacI G_AGCT'C
Cuts at: 2505 2505
Size: 5752
SacII CC_GC'GG
Cuts at: 2773 2773
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SanDI GG'GwC_CC
Cuts at: 2097 2097
Size: 5752
SbfI CC_TGCA'GG
Cuts at: 2314 3104 2314
Size: 790 4962
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 2310 2321 3100 4235 4916 5107 826
Size: 219 1265 11 779 1135 681 191 1471
Fragments arranged by size:
1471 1265 1135 779 681 219 191 11
SfoI GGC'GCC
Cuts at: 2083 2559 2083
Size: 476 5276
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 2178 1700
Size: 478 5274
SmaI CCC'GGG
Cuts at: 1872 2190 3079 1872
Size: 318 889 4545
Fragments arranged by size:
4545 889 318
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SrfI GCCC'GGGC
Cuts at: 2190 2190
Size: 5752
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 2214 2264 2316 2901 1517
Size: 697 50 52 585 4368
Fragments arranged by size:
4368 697 585 52 50
StyI C'CwwG_G
Cuts at: 513 1065 2004 2452 2463 2532 3191 513
Size: 552 939 448 11 69 659 3074
Fragments arranged by size:
3074 939 659 552 448 69 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2726 3779 3796 3949 4134 4579 325
Size: 1255 1146 1053 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1146 1053 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3998 5710 96
Size: 274 80 33 51 1443 2021 1712 138
Fragments arranged by size:
2021 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2231 2689 2231
Size: 458 5294
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 2188 3077 1870
Size: 318 889 4545
Fragments arranged by size:
4545 889 318
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII
SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PspXI PsrI PvuII RsrII SapI SexAI SfiI
SwaI