(Linear) (Six-base) MAPSORT of: KIEE2608.seq Check: 4411 from: 1 to: 1047 KIEE2608.orf With 182 enzymes: SgfI * February 23, 2016 17:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 600 1047 Size: 600 447 AfeI AGC'GCT Cuts at: 0 143 1047 Size: 143 904 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 320 1047 Size: 320 727 AlwNI CAG_nnn'CTG Cuts at: 0 277 516 592 1047 Size: 277 239 76 455 Fragments arranged by size: 455 277 239 76 ApaI G_GGCC'C Cuts at: 0 184 205 1047 Size: 184 21 842 Fragments arranged by size: 842 184 21 ApoI r'AATT_y Cuts at: 0 955 1047 Size: 955 92 AvaI C'yCGr_G Cuts at: 0 183 358 1006 1047 Size: 183 175 648 41 Fragments arranged by size: 648 183 175 41 BamHI G'GATC_C Cuts at: 0 1030 1047 Size: 1030 17 BanI G'GyrC_C Cuts at: 0 76 552 1047 Size: 76 476 495 Fragments arranged by size: 495 476 76 BanII G_rGCy'C Cuts at: 0 184 205 500 1007 1047 Size: 184 21 295 507 40 Fragments arranged by size: 507 295 184 40 21 BbeI G_GCGC'C Cuts at: 0 80 556 1047 Size: 80 476 491 Fragments arranged by size: 491 476 80 BbsI GAAGACnn'nnnn_ Cuts at: 0 722 1047 Size: 722 325 BbvCI CC'TCA_GC Cuts at: 0 278 1047 Size: 278 769 BglI GCCn_nnn'nGGC Cuts at: 0 74 179 1047 Size: 74 105 868 Fragments arranged by size: 868 105 74 BlpI GC'TnA_GC Cuts at: 0 64 1047 Size: 64 983 Bme1580I G_kGCm'C Cuts at: 0 184 205 1047 Size: 184 21 842 Fragments arranged by size: 842 184 21 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 555 1047 Size: 555 492 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 246 362 762 1047 Size: 79 167 116 400 285 Fragments arranged by size: 400 285 167 116 79 Bpu10I CC'TnA_GC Cuts at: 0 278 293 1047 Size: 278 15 754 Fragments arranged by size: 754 278 15 BsaHI Gr'CG_yC Cuts at: 0 77 151 553 1047 Size: 77 74 402 494 Fragments arranged by size: 494 402 77 74 BsaWI w'CCGG_w Cuts at: 0 962 1047 Size: 962 85 BseYI C'CCAG_C Cuts at: 0 4 138 214 472 638 1047 Size: 4 134 76 258 166 409 Fragments arranged by size: 409 258 166 134 76 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 427 861 1047 Size: 427 434 186 Fragments arranged by size: 434 427 186 BsiHKAI G_wGCw'C Cuts at: 0 416 500 1047 Size: 416 84 547 Fragments arranged by size: 547 416 84 BsmBI CGTCTCn'nnnn_ Cuts at: 0 414 1003 1047 Size: 414 589 44 Fragments arranged by size: 589 414 44 Bsp1286I G_dGCh'C Cuts at: 0 184 205 416 500 1007 1047 Size: 184 21 211 84 507 40 Fragments arranged by size: 507 211 184 84 40 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 338 600 869 1047 Size: 338 262 269 178 Fragments arranged by size: 338 269 262 178 BsrBI CCG'CTC Cuts at: 0 25 1047 Size: 25 1022 BsrDI GCAATG_nn' Cuts at: 0 346 1047 Size: 346 701 BsrFI r'CCGG_y Cuts at: 0 173 1047 Size: 173 874 BstAPI GCAn_nnn'nTGC Cuts at: 0 592 1047 Size: 592 455 BstEII G'GTnAC_C Cuts at: 0 240 1047 Size: 240 807 BstXI CCAn_nnnn'nTGG Cuts at: 0 146 549 1047 Size: 146 403 498 Fragments arranged by size: 498 403 146 BstYI r'GATC_y Cuts at: 0 1030 1047 Size: 1030 17 Bsu36I CC'TnA_GG Cuts at: 0 155 1047 Size: 155 892 BtgI C'CryG_G Cuts at: 0 527 765 1047 Size: 527 238 282 Fragments arranged by size: 527 282 238 EaeI y'GGCC_r Cuts at: 0 1013 1047 Size: 1013 34 EarI CTCTTCn'nnn_ Cuts at: 0 596 1047 Size: 596 451 EcoICRI GAG'CTC Cuts at: 0 498 1047 Size: 498 549 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 79 246 362 762 1047 Size: 79 167 116 400 285 Fragments arranged by size: 400 285 167 116 79 EcoNI CCTnn'n_nnAGG Cuts at: 0 316 1047 Size: 316 731 EcoO109I rG'GnC_Cy Cuts at: 0 92 180 201 327 469 1047 Size: 92 88 21 126 142 578 Fragments arranged by size: 578 142 126 92 88 21 HaeII r_GCGC'y Cuts at: 0 80 145 556 996 1047 Size: 80 65 411 440 51 Fragments arranged by size: 440 411 80 65 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 446 478 1015 1022 1047 Size: 446 32 537 7 25 Fragments arranged by size: 537 446 32 25 7 KasI G'GCGC_C Cuts at: 0 76 552 1047 Size: 76 476 495 Fragments arranged by size: 495 476 76 MslI CAynn'nnrTG Cuts at: 0 928 1047 Size: 928 119 MspA1I CmG'CkG Cuts at: 0 767 1047 Size: 767 280 NaeI GCC'GGC Cuts at: 0 175 1047 Size: 175 872 NarI GG'CG_CC Cuts at: 0 77 553 1047 Size: 77 476 494 Fragments arranged by size: 494 476 77 NcoI C'CATG_G Cuts at: 0 527 1047 Size: 527 520 NgoMIV G'CCGG_C Cuts at: 0 173 1047 Size: 173 874 NspI r_CATG'y Cuts at: 0 927 1047 Size: 927 120 PflMI CCAn_nnn'nTGG Cuts at: 0 516 1047 Size: 516 531 PpuMI rG'GwC_Cy Cuts at: 0 92 327 469 1047 Size: 92 235 142 578 Fragments arranged by size: 578 235 142 92 PspOMI G'GGCC_C Cuts at: 0 180 201 1047 Size: 180 21 846 Fragments arranged by size: 846 180 21 PstI C_TGCA'G Cuts at: 0 309 320 1047 Size: 309 11 727 Fragments arranged by size: 727 309 11 SacI G_AGCT'C Cuts at: 0 500 1047 Size: 500 547 SacII CC_GC'GG Cuts at: 0 768 1047 Size: 768 279 SanDI GG'GwC_CC Cuts at: 0 92 1047 Size: 92 955 SbfI CC_TGCA'GG Cuts at: 0 309 1047 Size: 309 738 SfcI C'TryA_G Cuts at: 0 305 316 1047 Size: 305 11 731 Fragments arranged by size: 731 305 11 SfoI GGC'GCC Cuts at: 0 78 554 1047 Size: 78 476 493 Fragments arranged by size: 493 476 78 SgrAI Cr'CCGG_yG Cuts at: 0 173 1047 Size: 173 874 SmaI CCC'GGG Cuts at: 0 185 1047 Size: 185 862 SrfI GCCC'GGGC Cuts at: 0 185 1047 Size: 185 862 StuI AGG'CCT Cuts at: 0 209 259 311 896 1047 Size: 209 50 52 585 151 Fragments arranged by size: 585 209 151 52 50 StyI C'CwwG_G Cuts at: 0 447 458 527 1047 Size: 447 11 69 520 Fragments arranged by size: 520 447 69 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 721 1047 Size: 721 326 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 226 684 1047 Size: 226 458 363 Fragments arranged by size: 458 363 226 XmaI C'CCGG_G Cuts at: 0 183 1047 Size: 183 864 Enzymes that do cut: AarI AfeI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BlpI Bme1580I BplI BpmI Bpu10I BsaHI BsaWI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I BtgI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PpuMI PspOMI PstI SacI SacII SanDI SbfI SfcI SfoI SgrAI SmaI SrfI StuI StyI TaqII XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BglII BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaXI BseRI BsiEI BsiWI BsmI BspEI BspHI BsrGI BssHII BssSI BstBI BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MscI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SalI SapI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI SspI SwaI TatI TstI Tth111I XbaI XhoI XmnI ZraI