################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BsaAI BsaBI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HindIII HpaI KasI KspI MamI MfeI MluI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 24 GGTACC G^GTACC AccII 85 CGCG CG^CG AcyI 16 GACGCC G[GA]^CG[CT]C AocI 123 CCTGAGG CC^T[ACGT]AGG Asp718I 24 GGTACC G^GTACC AspHI 874 GAGCTC G[AT]GC[AT]^C BamHI 984 GGATCC G^GATCC BanII 874 GAGCTC G[GA]GC[CT]^C BglII 399 AGATCT A^GATCT BlpI 756 GCTGAGC GC^T[ACGT]AGC BmyI 874 GAGCTC G[AGT]GC[ACT]^C Bpu1102I 756 GCTGAGC GC^T[ACGT]AGC BsaHI 16 GACGCC G[GA]^CG[CT]C BsaI 688 GAGACC GGTCTC(1/5) BsgI 728 CTGCAC GTGCAG(16/14) BsiHKAI 874 GAGCTC G[AT]GC[AT]^C BsmI 909 GCATTC GAATGC(1/-1) Bsp1286I 874 GAGCTC G[AGT]GC[ACT]^C BspMI 956 ACCTGC ACCTGC(4/8) BsrBI 868 GAGCGG CCGCTC(-3/-3) BstUI 85 CGCG CG^CG BstXI 803 CCAAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 123 CCTGAGG CC^T[ACGT]AGG CelII 756 GCTGAGC GC^T[ACGT]AGC DraII 431 AGGACCT [GA]G^G[ACGT]CC[CT] EarI 628 GAAGAG CTCTTC(1/4) Eco81I 123 CCTGAGG CC^T[ACGT]AGG EcoO109I 431 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 791 CCAAGG C^C[AT][AT]GG Hin1I 16 GACGCC G[GA]^CG[CT]C HincII 448 GTTGAC GT[CT]^[GA]AC HindII 448 GTTGAC GT[CT]^[GA]AC KpnI 24 GGTACC GGTAC^C Ksp632I 628 GAAGAG CTCTTC(1/4) MslI 201 CACCATAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 85 CGCG CG^CG PpuMI 431 AGGACCT [GA]G^G[AT]CC[CT] PshAI 168 GACACCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 874 GAGCTC GAGCT^C SexAI 948 ACCTGGT A^CC[AT]GGT StyI 791 CCAAGG C^C[AT][AT]GG TaqI 116 TCGA T^CGA Tsp509I 292 AATT ^AATT TthHB8I 116 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 233 GTCTAC GT^[AC][GT]AC 667 GTAGAC AccIII 44 TCCGGA T^CCGGA 917 TCCGGA AvaII 183 GGTCC G^G[AT]CC 432 GGACC BalI 149 TGGCCA TGG^CCA 380 TGGCCA BanI 24 GGTACC G^G[CT][GA]CC 199 GGCACC BcnI 11 CCGGG CC^[GC]GG 20 CCGGG BfaI 266 CTAG C^TAG 767 CTAG BsaWI 44 TCCGGA [AT]^CCGG[AT] 917 TCCGGA BseAI 44 TCCGGA T^CCGGA 917 TCCGGA BsmAI 688 GAGAC GTCTC(1/5) 847 GAGAC BspEI 44 TCCGGA T^CCGGA 917 TCCGGA BstAPI 567 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 900 GCAGAGCTTGC Cfr13I 183 GGTCC G^G[ACGT]CC 432 GGACC DraIII 94 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG 947 CACCTGGTG FauI 353 GCGGG CCCGC(4/6) 870 GCGGG MaeI 266 CTAG C^TAG 767 CTAG MaeII 483 ACGT A^CGT 701 ACGT MluNI 149 TGGCCA TGG^CCA 380 TGGCCA MroI 44 TCCGGA T^CCGGA 917 TCCGGA MscI 149 TGGCCA TGG^CCA 380 TGGCCA NciI 11 CCGGG CC^[GC]GG 20 CCGGG PflMI 28 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 588 CCAGGCTTTGG PvuII 388 CAGCTG CAG^CTG 577 CAGCTG Sau96I 183 GGTCC G^G[ACGT]CC 432 GGACC TfiI 31 GAATC G^A[AT]TC 963 GAATC TspRI 731 CACTG CA[GC]TG[ACGT][ACGT]^ 809 CACTG Van91I 28 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 588 CCAGGCTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI BbsI BbvI BpmI BpuAI BsaJI BseRI BsiYI BslI BsmFI BsrI BssKI BstEII BstF5I BstNI BstPI BstYI Cac8I CfoI DdeI DpnII EaeI EcoNI EcoO65I EcoRII Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NdeII NlaIV PleI PstI RsaI Sau3AI ScrFI SfaNI SfcI StuI TseI Tsp45I XhoII