################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DpnII DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MroI MslI MunI NaeI NarI NcoI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI Sau3AI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### ApaI 337 GGGCCC GGGCC^C AvaI 359 CCCGGG C^[CT]CG[GA]G AvaII 317 GGACC G^G[AT]CC BalI 368 TGGCCA TGG^CCA BanII 337 GGGCCC G[GA]GC[CT]^C BbsI 444 GAAGAC GAAGAC(2/6) BglI 227 GCCCCACCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BmyI 337 GGGCCC G[AGT]GC[ACT]^C BpuAI 444 GAAGAC GAAGAC(2/6) BsaI 61 GAGACC GGTCTC(1/5) BseRI 453 CTCCTC GAGGAG(10/8) BsiEI 234 CGGCCG CG[GA][CT]^CG BsmI 419 GAATGC GAATGC(1/-1) BsoBI 359 CCCGGG C^[CT]CG[GA]G Bsp1286I 337 GGGCCC G[AGT]GC[ACT]^C BsrFI 232 ACCGGC [GA]^CCGG[CT] BssHII 406 GCGCGC G^CGCGC Cfr10I 232 ACCGGC [GA]^CCGG[CT] DraII 337 GGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 194 CCACGG C^C[GA][CT]GG EagI 234 CGGCCG C^GGCCG EclXI 234 CGGCCG C^GGCCG Eco52I 234 CGGCCG C^GGCCG EcoO109I 337 GGGCCCC [GA]G^G[ACGT]CC[CT] MluNI 368 TGGCCA TGG^CCA MscI 368 TGGCCA TGG^CCA MseI 34 TTAA T^TAA NdeI 250 CATATG CA^TATG PshAI 63 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 426 CTGCAG CTGCA^G PvuII 18 CAGCTG CAG^CTG SapI 105 GAAGAGC GCTCTTC(1/4) SbfI 425 CCTGCAGG CCTGCA^GG SfaNI 150 GATGC GCATC(5/9) SfcI 426 CTGCAG C^T[GA][CT]AG SfiI 226 GGCCCCACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 359 CCCGGG CCC^GGG Sse8387I 425 CCTGCAGG CCTGCA^GG Tru9I 34 TTAA T^TAA TspRI 330 CAGTG CA[GC]TG[ACGT][ACGT]^ XmaCI 359 CCCGGG C^CCGGG XmaI 359 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AccII 405 CGCG CG^CG 407 CGCG Asp700I 432 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 444 GAAGACCTTC BsmAI 61 GAGAC GTCTC(1/5) 239 GAGAC BsrI 135 CCAGT ACTGG(1/-1) 329 CCAGT BstUI 405 CGCG CG^CG 407 CGCG Cac8I 245 GCGAGC GC[ACGT]^[ACGT]GC 406 GCGCGC EarI 58 GAAGAG CTCTTC(1/4) 105 GAAGAG Ksp632I 58 GAAGAG CTCTTC(1/4) 105 GAAGAG MspA1I 18 CAGCTG C[AC]G^C[GT]G 203 CCGCTG MvnI 405 CGCG CG^CG 407 CGCG XmnI 432 GAACTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 444 GAAGACCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BcnI BpmI BsaJI BsiYI BslI BsmFI BssKI BstNI CfoI Cfr13I EaeI EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIV Sau96I ScrFI TseI