##################################
# NO CUT ENZYMES (  214 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsmFI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       HphI        KasI        KpnI        KspI        
MaeII       MaeIII      MamI        MboI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MspA1I      MspI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NlaIV       NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      Sau96I      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AlwNI         386  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
BcgI           93  CGAGGAAGTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          322  CTAG                 C^TAG
BpmI          392  CTGGAG               CTGGAG(16/14)
BsaI           16  GAGACC               GGTCTC(1/5)
BsaJI          20  CCGAGG               C^C[ACGT][ACGT]GG
BsmAI          16  GAGAC                GTCTC(1/5)
BsrDI         138  CATTGC               GCAATG(2/0)
BssKI         257  CCAGG                ^CC[ACGT]GG
BstAPI        205  GCAAGACATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        357  GGATG                GGATG(2/0)
BstNI         257  CCAGG                CC^[AT]GG
Cac8I         381  GCCAGC               GC[ACGT]^[ACGT]GC
EaeI          143  CGGCCA               [CT]^GGCC[GA]
EarI          301  GAAGAG               CTCTTC(1/4)
EcoRII        257  CCAGG                ^CC[AT]GG
FokI          357  GGATG                GGATG(9/13)
HaeIII        144  GGCC                 GG^CC
HinfI          35  GATTC                G^A[ACGT]TC
Ksp632I       301  GAAGAG               CTCTTC(1/4)
MaeI          322  CTAG                 C^TAG
MvaI          257  CCAGG                CC^[AT]GG
MwoI          205  GCAAGACATGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        211  CATG                 CATG^
NspI          210  ACATGC               [GA]CATG^[CT]
ScrFI         257  CCAGG                CC^[ACGT]GG
SfaNI         117  GATGC                GCATC(5/9)
TfiI           35  GATTC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

AciI           13  GCGG                 CCGC(-3/-1)
              142  GCGG                
AcsI          177  AAATTT               [GA]^AATT[CT]
              369  AAATTC              
AluI          155  AGCT                 AG^CT
              248  AGCT                
ApoI          177  AAATTT               [GA]^AATT[CT]
              369  AAATTC              
DdeI          252  CTTAG                C^T[ACGT]AG
              311  CTCAG               
MseI          230  TTAA                 T^TAA
              317  TTAA                
Tru9I         230  TTAA                 T^TAA
              317  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    9 enzymes) #
#########################################

BbvI        BseRI       Fnu4HI      ItaI        MboII       
MnlI        TseI        Tsp509I     TspRI