(Linear) (Six-base) MAPSORT of: KIEE3705.seq  Check: 4440  from: 1  to: 414



KIEE3705.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 18:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     414 
   Size:        257     157
 
AlwNI CAG_nnn'CTG

Cuts at:      0     391     414 
   Size:        391      23
 
ApoI r'AATT_y

Cuts at:      0     177     369     414 
   Size:        177     192      45

  Fragments arranged by size: 

                 192     177      45
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      82     116     414 
   Size:         82      34     298

  Fragments arranged by size: 

                 298      82      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     414 
   Size:        413       1
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      10     414 
   Size:         10     404
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      19      22      37     276     414 
   Size:         19       3      15     239     138

  Fragments arranged by size: 

                 239     138      19      15       3
 
BsrDI GCAATG_nn'

Cuts at:      0     137     414 
   Size:        137     277
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     211     414 
   Size:        211     203
 
BtsI GCAGTG_nn'

Cuts at:      0     271     414 
   Size:        271     143
 
EaeI y'GGCC_r

Cuts at:      0     143     414 
   Size:        143     271
 
EarI CTCTTCn'nnn_

Cuts at:      0     296     414 
   Size:        296     118
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      28     414 
   Size:         28     386
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     413     414 
   Size:        257     156       1

  Fragments arranged by size: 

                 257     156       1
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     189     414 
   Size:        189     225
 
NspI r_CATG'y

Cuts at:      0     214     414 
   Size:        214     200
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     106     138     414 
   Size:        106      32     276

  Fragments arranged by size: 

                 276     106      32
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      34     414 
   Size:         34     380
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BcgI     BpmI     BsaI    BseRI    BsrDI
   BstAPI     BtsI     EaeI     EarI     EciI  Eco57MI     MmeI     NspI
     PpiI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI