(Linear) (Six-base) MAPSORT of: KIEE6167.seq  Check: 8255  from: 1  to: 402



KIEE6167.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:27  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     402 
   Size:        130     272
 
AlwNI CAG_nnn'CTG

Cuts at:      0     317     402 
   Size:        317      85
 
ApoI r'AATT_y

Cuts at:      0     190     208     402 
   Size:        190      18     194

  Fragments arranged by size: 

                 194     190      18
 
AvrII C'CTAG_G

Cuts at:      0      69     402 
   Size:         69     333
 
BbvCI CC'TCA_GC

Cuts at:      0      13     402 
   Size:         13     389
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     298     402 
   Size:        298     104
 
BlpI GC'TnA_GC

Cuts at:      0      34     402 
   Size:         34     368
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     361     402 
   Size:        361      41
 
Bpu10I CC'TnA_GC

Cuts at:      0      13     402 
   Size:         13     389
 
Bsu36I CC'TnA_GG

Cuts at:      0     172     402 
   Size:        172     230
 
DraI TTT'AAA

Cuts at:      0     195     402 
   Size:        195     207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     130     402 
   Size:        130     272
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     402 
   Size:        180     222
 
MspA1I CmG'CkG

Cuts at:      0      17     402 
   Size:         17     385
 
NspI r_CATG'y

Cuts at:      0      63     402 
   Size:         63     339
 
StuI AGG'CCT

Cuts at:      0     230     402 
   Size:        230     172
 
StyI C'CwwG_G

Cuts at:      0      69     402 
   Size:         69     333
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    AvrII    BbvCI    BciVI     BlpI     BplI
   Bpu10I   Bsu36I     DraI  Eco57MI     MmeI   MspA1I     NspI     StuI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
     BbsI     BcgI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI