Restriction map for
pFN21AE1579
(Circular) (Six-base) MAPSORT of: pFN21AE1579.seq Check: 3503 from: 1 to: 4948
pFN21AE1579 4948 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2758 278
Size: 53 83 186 936 1222 2468
Fragments arranged by size:
2468 1222 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2291 1937
Size: 354 4594
Acc65I G'GTAC_C
Cuts at: 2269 2269
Size: 4948
AclI AA'CG_TT
Cuts at: 1563 3075 3448 1563
Size: 1512 373 3063
Fragments arranged by size:
3063 1512 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3011 4395 4663 1632
Size: 1379 1384 268 1917
Fragments arranged by size:
1917 1384 1379 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4751
AflIII A'CryG_T
Cuts at: 1203 3847 4824 1203
Size: 2644 977 1327
Fragments arranged by size:
2644 1327 977
AgeI A'CCGG_T
Cuts at: 3779 3779
Size: 4948
AhdI GACnn_n'nnGTC
Cuts at: 3677 3677
Size: 4948
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4948
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4948
AlwNI CAG_nnn'CTG
Cuts at: 2691 4263 2691
Size: 1572 3376
ApaLI G'TGCA_C
Cuts at: 3005 4161 3005
Size: 1156 3792
ApoI r'AATT_y
Cuts at: 1493 2257 2483 2635 4744 1493
Size: 764 226 152 2109 1697
Fragments arranged by size:
2109 1697 764 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3502 160
Size: 872 2470 1606
Fragments arranged by size:
2470 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4948
AvaI C'yCGr_G
Cuts at: 1870 1943 2024 2273 1870
Size: 73 81 249 4545
Fragments arranged by size:
4545 249 81 73
BamHI G'GATC_C
Cuts at: 2278 2645 2278
Size: 367 4581
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2269 3724 618
Size: 325 217 627 482 1455 1842
Fragments arranged by size:
1842 1455 627 482 325 217
BanII G_rGCy'C
Cuts at: 1391 2023 2267 1391
Size: 632 244 4072
Fragments arranged by size:
4072 632 244
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2241 928
Size: 971 342 3635
Fragments arranged by size:
3635 971 342
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2174 2208 3139 3173 1903
Size: 34 237 34 931 34 3678
Fragments arranged by size:
3678 931 237 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2840 4050 2840
Size: 1210 3738
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4948
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3559 136
Size: 107 122 71 631 605 1887 1525
Fragments arranged by size:
1887 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4943 4943
Size: 4948
BlpI GC'TnA_GC
Cuts at: 2159 2365 2159
Size: 206 4742
Bme1580I G_kGCm'C
Cuts at: 1790 3009 4165 1790
Size: 1219 1156 2573
Fragments arranged by size:
2573 1219 1156
BmgBI CAC'GTC
Cuts at: 1318 2203 1318
Size: 885 4063
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2679 3637 442
Size: 416 53 83 399 24 1262 958 1753
Fragments arranged by size:
1753 1262 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4948
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2090 2090
Size: 4948
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3608 1239
Size: 2369 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3073 3938 4236 3073
Size: 865 298 3785
Fragments arranged by size:
3785 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3611 882
Size: 617 2112 2219
Fragments arranged by size:
2219 2112 617
BsaAI yAC'GTr
Cuts at: 493 1204 2739 493
Size: 711 1535 2702
Fragments arranged by size:
2702 1535 711
BsaBI GATnn'nnATC
Cuts at: 4942 4942
Size: 4948
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2755 3137 3772 275
Size: 53 83 186 936 1222 382 635 1451
Fragments arranged by size:
1451 1222 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2035 2238 3381 3779 4053 4200 4611 2035
Size: 203 1143 398 274 147 411 2372
Fragments arranged by size:
2372 1143 411 398 274 203 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2030 2060 1178
Size: 30 165 30 627 30 4066
Fragments arranged by size:
4066 627 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2129 2132 1186
Size: 868 75 3 4002
Fragments arranged by size:
4002 868 75 3
BseYI C'CCAG_C
Cuts at: 4151 4647 4151
Size: 496 4452
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2214 1163
Size: 573 478 3897
Fragments arranged by size:
3897 573 478
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2398 3159 4187 1540
Size: 275 186 397 761 1028 2301
Fragments arranged by size:
2301 1028 761 397 275 186
BsiHKAI G_wGCw'C
Cuts at: 2023 3009 3094 4165 2023
Size: 986 85 1071 2806
Fragments arranged by size:
2806 1071 986 85
BsmI GAATG_Cn'
Cuts at: 2459 2552 2459
Size: 93 4855
BsmBI CGTCTCn'nnnn_
Cuts at: 2222 2222
Size: 4948
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2023 2267 3009 3094 4165 1391
Size: 399 233 244 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 244 233 85
BspEI T'CCGG_A
Cuts at: 2035 4611 2035
Size: 2576 2372
BspHI T'CATG_A
Cuts at: 2837 4567 2837
Size: 1730 3218
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2236 2303 844
Size: 1392 67 3489
Fragments arranged by size:
3489 1392 67
BsrBI CCG'CTC
Cuts at: 1394 1823 2835 1394
Size: 429 1012 3507
Fragments arranged by size:
3507 1012 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3443 3617 65
Size: 1183 2195 174 1396
Fragments arranged by size:
2195 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3592 3779 1700
Size: 110 1782 187 2869
Fragments arranged by size:
2869 1782 187 110
BsrGI T'GTAC_A
Cuts at: 96 2046 96
Size: 1950 2998
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4948
BssSI C'ACGA_G
Cuts at: 3008 4020 3008
Size: 1012 3936
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4948
BstBI TT'CG_AA
Cuts at: 3752 3752
Size: 4948
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4948
BstYI r'GATC_y
Cuts at: 1972 2278 2645 3030 3047 4499 4585 4597 4943
Size: 306 367 385 17 1452 86 12 346
Cuts at: 4943 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 306 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4396
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4209
Fragments arranged by size:
4209 683 56
BtsI GCAGTG_nn'
Cuts at: 2464 3258 3278 2464
Size: 794 20 4134
Fragments arranged by size:
4134 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4948
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4913
DraI TTT'AAA
Cuts at: 2252 2252
Size: 4948
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3955 809
Size: 507 2639 1802
Fragments arranged by size:
2639 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2395 3284 4913
Size: 54 1425 50 275 18 565 889 1629
Cuts at: 4913 8
Size: 43
Fragments arranged by size:
1629 1425 889 565 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2395 1537
Size: 275 583 4090
Fragments arranged by size:
4090 583 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2024 2878 1353
Size: 671 854 3423
Fragments arranged by size:
3423 854 671
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3535 3909 4055 387
Size: 1064 138 1946 374 146 1280
Fragments arranged by size:
1946 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2021 2021
Size: 4948
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3011 3608 4395 4663 1239
Size: 393 1379 597 787 268 1524
Fragments arranged by size:
1524 1379 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2257 2635 2257
Size: 378 4570
FspI TGC'GCA
Cuts at: 2129 3454 2129
Size: 1325 3623
HaeII r_GCGC'y
Cuts at: 1678 4095 1678
Size: 2417 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3589 3621 3663 3695 1373
Size: 32 135 32 2017 32 42 32 2626
Fragments arranged by size:
2626 2017 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2149 2292 2538 669
Size: 988 281 211 143 246 3079
Fragments arranged by size:
3079 988 281 246 211 143
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4948
HpaI GTT'AAC
Cuts at: 2538 2538
Size: 4948
KpnI G_GTAC'C
Cuts at: 2273 2273
Size: 4948
MfeI C'AATT_G
Cuts at: 2547 2547
Size: 4948
MluI A'CGCG_T
Cuts at: 4824 4824
Size: 4948
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2205 4062 4246 1305
Size: 900 1857 184 2007
Fragments arranged by size:
2007 1857 900 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3126
Fragments arranged by size:
3126 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2906 3265 3424 518
Size: 648 1740 359 159 2042
Fragments arranged by size:
2042 1740 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2167 2364 3041 4189 4434 1606
Size: 561 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 561 245 197
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4396
NdeI CA'TA_TG
Cuts at: 387 387
Size: 4948
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4948
NotI GC'GGCC_GC
Cuts at: 2395 2395
Size: 4948
NspI r_CATG'y
Cuts at: 1132 1229 2306 3851 1132
Size: 97 1077 1545 2229
Fragments arranged by size:
2229 1545 1077 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4948
PciI A'CATG_T
Cuts at: 3847 3847
Size: 4948
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4948
PmeI GTTT'AAAC
Cuts at: 2252 2252
Size: 4948
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2105 2137 3017 3049 4530 4562 2105
Size: 32 880 32 1481 32 2491
Fragments arranged by size:
2491 1481 880 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4948
PsiI TTA'TAA
Cuts at: 2518 2518
Size: 4948
PstI C_TGCA'G
Cuts at: 830 2300 830
Size: 1470 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4948
PvuII CAG'CTG
Cuts at: 2167 2167
Size: 4948
SacI G_AGCT'C
Cuts at: 2023 2023
Size: 4948
SalI G'TCGA_C
Cuts at: 1936 2290 1936
Size: 354 4594
SbfI CC_TGCA'GG
Cuts at: 2300 2300
Size: 4948
ScaI AGT'ACT
Cuts at: 3196 4908 3196
Size: 1712 3236
SfcI C'TryA_G
Cuts at: 826 1045 2296 3431 4112 4303 826
Size: 219 1251 1135 681 191 1471
Fragments arranged by size:
1471 1251 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4948
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4948
SmaI CCC'GGG
Cuts at: 1872 2026 2275 1872
Size: 154 249 4545
Fragments arranged by size:
4545 249 154
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3052 3953 4215 820
Size: 197 926 1109 901 262 1553
Fragments arranged by size:
1553 1109 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2739 493
Size: 2246 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4948
SphI G_CATG'C
Cuts at: 1132 2306 1132
Size: 1174 3774
SspI AAT'ATT
Cuts at: 5 52 2872 5
Size: 47 2820 2081
Fragments arranged by size:
2820 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4948
StyI C'CwwG_G
Cuts at: 513 1065 2387 513
Size: 552 1322 3074
Fragments arranged by size:
3074 1322 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2975 2992 3145 3330 3775 325
Size: 1255 1395 17 153 185 445 1498
Fragments arranged by size:
1498 1395 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2046 3194 4906
Size: 274 80 33 51 1443 69 1148 1712
Cuts at: 4906 96
Size: 138
Fragments arranged by size:
1712 1443 1148 274 138 80 69 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4908 4940 4908
Size: 32 4916
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4948
XbaI T'CTAG_A
Cuts at: 2284 2284
Size: 4948
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4948
XmaI C'CCGG_G
Cuts at: 1870 2024 2273 1870
Size: 154 249 4545
Fragments arranged by size:
4545 249 154
XmnI GAAnn'nnTTC
Cuts at: 3077 3077
Size: 4948
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2756 276
Size: 53 83 186 936 1222 2468
Fragments arranged by size:
2468 1222 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI