################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbsI BbvCI BcgI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstPI BstUI Bsu36I CelII ClaI CpoI DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboII MfeI MluI MluNI MroI MscI MseI MunI MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 276 GTATAC GT^[AC][GT]AC AcyI 361 GACGCC G[GA]^CG[CT]C AflIII 293 ACGTGT A^C[GA][CT]GT AvaI 694 CCCGAG C^[CT]CG[GA]G AvaII 45 GGACC G^G[AT]CC BanII 73 GAGCTC G[GA]GC[CT]^C BciVI 627 GTATCC GTATCC(6/5) BcnI 104 CCCGG CC^[GC]GG BglI 99 GCCCACCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 361 GACGCC G[GA]^CG[CT]C BsaWI 234 TCCGGT [AT]^CCGG[AT] BseRI 808 CTCCTC GAGGAG(10/8) BsoBI 694 CCCGAG C^[CT]CG[GA]G BsrBI 706 CCGCTC CCGCTC(-3/-3) Bst1107I 276 GTATAC GTA^TAC BstXI 101 CCACCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 276 GTATAC GTA^TAC DdeI 562 CTGAG C^T[ACGT]AG DraIII 334 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 63 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 324 GCGGG CCCGC(4/6) HaeII 755 AGCGCC [GA]GCGC^[CT] Hin1I 361 GACGCC G[GA]^CG[CT]C HphI 225 TCACC GGTGA(8/7) MaeIII 13 GTCAC ^GT[ACGT]AC MslI 588 CATGGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 786 GCCGGC GCC^GGC NciI 104 CCCGG CC^[GC]GG NgoMIV 786 GCCGGC G^CCGGC NspI 125 ACATGC [GA]CATG^[CT] PflMI 78 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 44 GGGACCC [GA]G^G[AT]CC[CT] PvuI 181 CGATCG CGAT^CG PvuII 151 CAGCTG CAG^CTG SacI 73 GAGCTC GAGCT^C SfaNI 231 GCATC GCATC(5/9) SfcI 632 CTGTAG C^T[GA][CT]AG StuI 800 AGGCCT AGG^CCT TaqI 689 TCGA T^CGA Tsp45I 13 GTCAC ^GT[GC]AC TthHB8I 689 TCGA T^CGA Van91I 78 CCAGGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AlwNI 79 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG 151 CAGCTGCTG AspHI 73 GAGCTC G[AT]GC[AT]^C 246 GAGCAC BbrPI 292 CACGTG CAC^GTG 306 CACGTG BpmI 76 CTCCAG CTGGAG(16/14) 17 CTGGAG BsgI 289 CTGCAC GTGCAG(16/14) 370 CTGCAC BsiEI 177 CGACCG CG[GA][CT]^CG 181 CGATCG BsiHKAI 73 GAGCTC G[AT]GC[AT]^C 246 GAGCAC BsmAI 318 GAGAC GTCTC(1/5) 505 GAGAC BsmI 668 GAATGC GAATGC(1/-1) 159 GCATTC BsrI 16 ACTGG ACTGG(1/-1) 120 ACTGG BssSI 409 CACGAG CACGAG(-5/-1) 394 CTCGTG BstF5I 232 CATCC GGATG(2/0) 576 GGATG Cac8I 95 GCAAGC GC[ACGT]^[ACGT]GC 786 GCCGGC DraII 44 GGGACCC [GA]G^G[ACGT]CC[CT] 58 AGGCCCC DsaI 282 CCGTGG C^C[GA][CT]GG 338 CCGTGG EcoO109I 44 GGGACCC [GA]G^G[ACGT]CC[CT] 58 AGGCCCC FokI 232 CATCC GGATG(9/13) 576 GGATG HgaI 253 GACGC GACGC(5/10) 361 GACGC PmaCI 292 CACGTG CAC^GTG 306 CACGTG PmlI 292 CACGTG CAC^GTG 306 CACGTG SmlI 610 CTCAAG C^T[CT][GA]AG 796 CTCAAG TspRI 15 CACTG CA[GC]TG[ACGT][ACGT]^ 655 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AfaI AluI AlwI BbvI BmyI BsaAI BsaJI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstNI BstYI CfoI Cfr10I Cfr13I DpnII EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboI MflI MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI TseI XhoII