(Circular) (Six-base) MAPSORT of: pFN21AE3976.seq Check: 805 from: 1 to: 5524 pFN21AE3976 5524 bp With 182 enzymes: SgfI * March 28, 2012 11:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2282 2867 1937 Size: 345 585 4594 Fragments arranged by size: 4594 585 345 Acc65I G'GTAC_C Cuts at: 2845 2845 Size: 5524 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2090 3587 4971 5239 1632 Size: 458 1497 1384 268 1917 Fragments arranged by size: 1917 1497 1384 458 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 2298 4423 5400 1203 Size: 1095 2125 977 1327 Fragments arranged by size: 2125 1327 1095 977 AgeI A'CCGG_T Cuts at: 4355 4355 Size: 5524 AhdI GACnn_n'nnGTC Cuts at: 4253 4253 Size: 5524 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5524 AlwNI CAG_nnn'CTG Cuts at: 2089 2161 3267 4839 2089 Size: 72 1106 1572 2774 Fragments arranged by size: 2774 1572 1106 72 ApaLI G'TGCA_C Cuts at: 3581 4737 3581 Size: 1156 4368 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4078 160 Size: 872 3046 1606 Fragments arranged by size: 3046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2699 2849 1870 Size: 73 756 150 4545 Fragments arranged by size: 4545 756 150 73 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2845 4300 618 Size: 325 217 627 1058 1455 1842 Fragments arranged by size: 1842 1455 1058 627 325 217 BanII G_rGCy'C Cuts at: 1391 2082 2843 1391 Size: 691 761 4072 Fragments arranged by size: 4072 761 691 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4553 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3715 3749 1903 Size: 34 1778 34 3678 Fragments arranged by size: 3678 1778 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2643 3416 4626 2643 Size: 773 1210 3541 Fragments arranged by size: 3541 1210 773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2110 4135 136 Size: 107 122 71 631 605 438 2025 1525 Fragments arranged by size: 2025 1525 631 605 438 122 107 71 BglII A'GATC_T Cuts at: 5519 5519 Size: 5524 BlpI GC'TnA_GC Cuts at: 2941 2941 Size: 5524 Bme1580I G_kGCm'C Cuts at: 1790 2336 3585 4741 1790 Size: 546 1249 1156 2573 Fragments arranged by size: 2573 1249 1156 546 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3255 4213 442 Size: 416 53 83 399 24 1838 958 1753 Fragments arranged by size: 1838 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2043 2066 4184 1239 Size: 804 23 2118 2579 Fragments arranged by size: 2579 2118 804 23 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2600 2786 3649 4514 4812 2600 Size: 186 863 865 298 3312 Fragments arranged by size: 3312 865 863 298 186 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4187 882 Size: 617 2688 2219 Fragments arranged by size: 2688 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2058 2299 2313 3315 493 Size: 711 854 241 14 1002 2702 Fragments arranged by size: 2702 1002 854 711 241 14 BsaBI GATnn'nnATC Cuts at: 5518 5518 Size: 5524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2367 3331 3713 4348 Size: 53 83 186 936 834 964 382 635 Cuts at: 4348 275 Size: 1451 Fragments arranged by size: 1451 964 936 834 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2239 3957 4355 4629 4776 5187 2239 Size: 1718 398 274 147 411 2576 Fragments arranged by size: 2576 1718 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5299 Fragments arranged by size: 5299 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2804 1186 Size: 1618 3906 BseYI C'CCAG_C Cuts at: 2427 2488 4727 5223 2427 Size: 61 2239 496 2728 Fragments arranged by size: 2728 2239 496 61 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2279 2360 1163 Size: 573 543 81 4327 Fragments arranged by size: 4327 573 543 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2185 2189 2974 3735 4763 1540 Size: 275 186 184 4 785 761 1028 2301 Fragments arranged by size: 2301 1028 785 761 275 186 184 4 BsiHKAI G_wGCw'C Cuts at: 2082 2255 3585 3670 4741 2082 Size: 173 1330 85 1071 2865 Fragments arranged by size: 2865 1330 1071 173 85 BsmI GAATG_Cn' Cuts at: 2164 2679 3035 3128 2164 Size: 515 356 93 4560 Fragments arranged by size: 4560 515 356 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2255 2336 2843 3585 3670 4741 Size: 399 292 173 81 507 742 85 1071 Cuts at: 4741 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 507 399 292 173 85 81 BspEI T'CCGG_A Cuts at: 5187 5187 Size: 5524 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2879 844 Size: 2035 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2713 3411 1394 Size: 429 890 698 3507 Fragments arranged by size: 3507 890 698 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4019 4193 65 Size: 1183 2771 174 1396 Fragments arranged by size: 2771 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2232 2445 2791 4168 4355 1700 Size: 110 422 213 346 1377 187 2869 Fragments arranged by size: 2869 1377 422 346 213 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5524 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5524 BssSI C'ACGA_G Cuts at: 2399 2414 3584 4596 2399 Size: 15 1170 1012 3327 Fragments arranged by size: 3327 1170 1012 15 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5524 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5524 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2113 Size: 5524 BstYI r'GATC_y Cuts at: 1972 2116 2667 2772 2854 3221 3606 3623 5075 Size: 144 551 105 82 367 385 17 1452 Cuts at: 5075 5161 5173 5519 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 551 385 367 346 144 105 86 82 17 12 BstZ17I GTA'TAC Cuts at: 2283 2283 Size: 5524 BtgI C'CryG_G Cuts at: 513 1065 2004 2287 2343 513 Size: 552 939 283 56 3694 Fragments arranged by size: 3694 939 552 283 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4785 Fragments arranged by size: 4785 683 56 BtsI GCAGTG_nn' Cuts at: 3040 3834 3854 3040 Size: 794 20 4710 Fragments arranged by size: 4710 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2382 2417 529 Size: 35 1818 35 3636 Fragments arranged by size: 3636 1818 35 35 DraI TTT'AAA Cuts at: 2828 2828 Size: 5524 DraIII CAC_nnn'GTG Cuts at: 2344 2344 Size: 5524 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4531 809 Size: 507 3215 1802 Fragments arranged by size: 3215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2971 3860 5489 Size: 54 1425 50 275 18 1141 889 1629 Cuts at: 5489 8 Size: 43 Fragments arranged by size: 1629 1425 1141 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2971 1537 Size: 275 1159 4090 Fragments arranged by size: 4090 1159 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3454 1353 Size: 2101 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4111 4485 4631 387 Size: 1064 138 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2080 2080 Size: 5524 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2043 2066 2090 3587 4184 4971 5239 Size: 393 411 23 24 1497 597 787 268 Cuts at: 5239 1239 Size: 1524 Fragments arranged by size: 1524 1497 787 597 411 393 268 24 23 EcoNI CCTnn'n_nnAGG Cuts at: 2072 2819 2072 Size: 747 4777 EcoO109I rG'GnC_Cy Cuts at: 2050 2064 2050 Size: 14 5510 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2242 2242 Size: 5524 FspI TGC'GCA Cuts at: 4030 4030 Size: 5524 HaeII r_GCGC'y Cuts at: 1678 2764 4671 1678 Size: 1086 1907 2531 Fragments arranged by size: 2531 1907 1086 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373 Size: 32 135 32 2593 32 42 32 2626 Fragments arranged by size: 2626 2593 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2868 3114 669 Size: 988 281 930 246 3079 Fragments arranged by size: 3079 988 930 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5524 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KpnI G_GTAC'C Cuts at: 2849 2849 Size: 5524 MfeI C'AATT_G Cuts at: 3123 3123 Size: 5524 MluI A'CGCG_T Cuts at: 5400 5400 Size: 5524 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4638 4822 1305 Size: 3333 184 2007 Fragments arranged by size: 3333 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3702 Fragments arranged by size: 3702 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2597 3482 3841 4000 518 Size: 648 1431 885 359 159 2042 Fragments arranged by size: 2042 1431 885 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2329 2707 2788 2940 3617 4765 5010 Size: 552 171 378 81 152 677 1148 245 Cuts at: 5010 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 552 378 245 171 152 81 NaeI GCC'GGC Cuts at: 2793 2793 Size: 5524 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4033 Fragments arranged by size: 4033 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5524 NgoMIV G'CCGG_C Cuts at: 2791 2791 Size: 5524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2971 2971 Size: 5524 NspI r_CATG'y Cuts at: 1132 1229 2134 2882 4427 1132 Size: 97 905 748 1545 2229 Fragments arranged by size: 2229 1545 905 748 97 PasI CC'CwG_GG Cuts at: 1348 2749 1348 Size: 1401 4123 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 2089 1108 Size: 981 4543 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PmlI CAC'GTG Cuts at: 2299 2313 2299 Size: 14 5510 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 5106 5138 3593 Size: 32 1481 32 3979 Fragments arranged by size: 3979 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2050 2050 Size: 5524 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5524 PsiI TTA'TAA Cuts at: 3094 3094 Size: 5524 PstI C_TGCA'G Cuts at: 830 2876 830 Size: 2046 3478 PvuI CG_AT'CG Cuts at: 2001 2189 2001 Size: 188 5336 PvuII CAG'CTG Cuts at: 2158 2158 Size: 5524 SacI G_AGCT'C Cuts at: 2082 2082 Size: 5524 SalI G'TCGA_C Cuts at: 1936 2866 1936 Size: 930 4594 SanDI GG'GwC_CC Cuts at: 2050 2050 Size: 5524 SbfI CC_TGCA'GG Cuts at: 2876 2876 Size: 5524 ScaI AGT'ACT Cuts at: 3772 5484 3772 Size: 1712 3812 SexAI A'CCwGG_T Cuts at: 2820 2820 Size: 5524 SfcI C'TryA_G Cuts at: 826 1045 2637 2872 4007 4688 4879 826 Size: 219 1592 235 1135 681 191 1471 Fragments arranged by size: 1592 1471 1135 681 235 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5524 SmaI CCC'GGG Cuts at: 1872 2851 1872 Size: 979 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2615 2801 3628 4529 4791 820 Size: 197 926 672 186 827 901 262 1553 Fragments arranged by size: 1553 926 901 827 672 262 197 186 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2882 1132 Size: 1750 3774 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 2807 1517 Size: 1290 4234 StyI C'CwwG_G Cuts at: 513 1065 2004 2963 513 Size: 552 939 959 3074 Fragments arranged by size: 3074 959 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2199 3551 3568 3721 3906 4351 325 Size: 1255 619 1352 17 153 185 445 1498 Fragments arranged by size: 1498 1352 1255 619 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2589 3770 5482 Size: 274 80 33 51 1443 612 1181 1712 Cuts at: 5482 96 Size: 138 Fragments arranged by size: 1712 1443 1181 612 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5484 5516 5484 Size: 32 5492 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5524 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5524 XmaI C'CCGG_G Cuts at: 1870 2849 1870 Size: 979 4545 XmnI GAAnn'nnTTC Cuts at: 3653 3653 Size: 5524 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI XcmI