Restriction map for
pFN21AE3976
(Linear) (Six-base) MAPSORT of: KIEE3976.seq Check: 1636 from: 1 to: 819
KIEE3976.orf
With 182 enzymes: SgfI *
March 22, 2012 17:02 ..
AccI GT'mk_AC
Cuts at: 0 277 819
Size: 277 542
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 819
Size: 85 734
AflIII A'CryG_T
Cuts at: 0 293 819
Size: 293 526
AlwNI CAG_nnn'CTG
Cuts at: 0 84 156 819
Size: 84 72 663
Fragments arranged by size:
663 84 72
AvaI C'yCGr_G
Cuts at: 0 694 819
Size: 694 125
BanII G_rGCy'C
Cuts at: 0 77 819
Size: 77 742
BciVI GTATCCnnnnn_n'
Cuts at: 0 638 819
Size: 638 181
BglI GCCn_nnn'nGGC
Cuts at: 0 105 819
Size: 105 714
Bme1580I G_kGCm'C
Cuts at: 0 331 819
Size: 331 488
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 61 819
Size: 38 23 758
Fragments arranged by size:
758 38 23
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 781 819
Size: 595 186 38
Fragments arranged by size:
595 186 38
BsaAI yAC'GTr
Cuts at: 0 53 294 308 819
Size: 53 241 14 511
Fragments arranged by size:
511 241 53 14
BsaHI Gr'CG_yC
Cuts at: 0 362 819
Size: 362 457
BsaWI w'CCGG_w
Cuts at: 0 234 819
Size: 234 585
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 799 819
Size: 799 20
BseYI C'CCAG_C
Cuts at: 0 422 483 819
Size: 422 61 336
Fragments arranged by size:
422 336 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 355 819
Size: 274 81 464
Fragments arranged by size:
464 274 81
BsiEI CG_ry'CG
Cuts at: 0 180 184 819
Size: 180 4 635
Fragments arranged by size:
635 180 4
BsiHKAI G_wGCw'C
Cuts at: 0 77 250 819
Size: 77 173 569
Fragments arranged by size:
569 173 77
BsmI GAATG_Cn'
Cuts at: 0 159 674 819
Size: 159 515 145
Fragments arranged by size:
515 159 145
Bsp1286I G_dGCh'C
Cuts at: 0 77 250 331 819
Size: 77 173 81 488
Fragments arranged by size:
488 173 81 77
BsrBI CCG'CTC
Cuts at: 0 708 819
Size: 708 111
BsrFI r'CCGG_y
Cuts at: 0 227 440 786 819
Size: 227 213 346 33
Fragments arranged by size:
346 227 213 33
BssSI C'ACGA_G
Cuts at: 0 394 409 819
Size: 394 15 410
Fragments arranged by size:
410 394 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 819
Size: 108 711
BstYI r'GATC_y
Cuts at: 0 111 662 767 819
Size: 111 551 105 52
Fragments arranged by size:
551 111 105 52
BstZ17I GTA'TAC
Cuts at: 0 278 819
Size: 278 541
BtgI C'CryG_G
Cuts at: 0 282 338 819
Size: 282 56 481
Fragments arranged by size:
481 282 56
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 377 412 819
Size: 377 35 407
Fragments arranged by size:
407 377 35
DraIII CAC_nnn'GTG
Cuts at: 0 339 819
Size: 339 480
EcoICRI GAG'CTC
Cuts at: 0 75 819
Size: 75 744
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 61 85 819
Size: 38 23 24 734
Fragments arranged by size:
734 38 24 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 67 819
Size: 67 752
EcoO109I rG'GnC_Cy
Cuts at: 0 45 59 819
Size: 45 14 760
Fragments arranged by size:
760 45 14
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 237 819
Size: 237 582
HaeII r_GCGC'y
Cuts at: 0 759 819
Size: 759 60
MslI CAynn'nnrTG
Cuts at: 0 592 819
Size: 592 227
MspA1I CmG'CkG
Cuts at: 0 153 324 702 783 819
Size: 153 171 378 81 36
Fragments arranged by size:
378 171 153 81 36
NaeI GCC'GGC
Cuts at: 0 788 819
Size: 788 31
NgoMIV G'CCGG_C
Cuts at: 0 786 819
Size: 786 33
NspI r_CATG'y
Cuts at: 0 129 819
Size: 129 690
PasI CC'CwG_GG
Cuts at: 0 744 819
Size: 744 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 84 819
Size: 84 735
PmlI CAC'GTG
Cuts at: 0 294 308 819
Size: 294 14 511
Fragments arranged by size:
511 294 14
PpuMI rG'GwC_Cy
Cuts at: 0 45 819
Size: 45 774
PvuI CG_AT'CG
Cuts at: 0 184 819
Size: 184 635
PvuII CAG'CTG
Cuts at: 0 153 819
Size: 153 666
SacI G_AGCT'C
Cuts at: 0 77 819
Size: 77 742
SanDI GG'GwC_CC
Cuts at: 0 45 819
Size: 45 774
SfcI C'TryA_G
Cuts at: 0 632 819
Size: 632 187
SmlI C'TyrA_G
Cuts at: 0 610 796 819
Size: 610 186 23
Fragments arranged by size:
610 186 23
StuI AGG'CCT
Cuts at: 0 802 819
Size: 802 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 194 819
Size: 194 625
TatI w'GTAC_w
Cuts at: 0 584 819
Size: 584 235
Enzymes that do cut:
AccI AcuI AflIII AlwNI AvaI BanII BciVI BglI
Bme1580I BpmI BpuEI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrBI BsrFI BssSI
BstXI BstYI BstZ17I BtgI CspCI DraIII EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII MslI MspA1I NaeI NgoMIV
NspI PasI PflMI PmlI PpuMI PvuI PvuII SacI
SanDI SfcI SmlI StuI TaqII TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaBI BsaXI BsiWI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssHII BstAPI BstBI BstEII
Bsu36I BtgZI BtsI ClaI DraI DrdI EaeI EagI
EarI EciI EcoRI EcoRV FseI FspI FspAI Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StyI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI