(Circular) (Six-base) MAPSORT of: pFN21AE2461.seq Check: 302 from: 1 to: 6466 pFN21AE2461 6466 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2149 2149 Size: 6466 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4276 278 Size: 53 83 186 936 2740 2468 Fragments arranged by size: 2740 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3588 3809 1937 Size: 1651 221 4594 Fragments arranged by size: 4594 1651 221 Acc65I G'GTAC_C Cuts at: 2301 3238 3787 2301 Size: 937 549 4980 Fragments arranged by size: 4980 937 549 AclI AA'CG_TT Cuts at: 1563 4593 4966 1563 Size: 3030 373 3063 Fragments arranged by size: 3063 3030 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3517 4529 5913 6181 1632 Size: 1885 1012 1384 268 1917 Fragments arranged by size: 1917 1885 1384 1012 268 AfeI AGC'GCT Cuts at: 3374 3374 Size: 6466 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6269 AflIII A'CryG_T Cuts at: 1203 5365 6342 1203 Size: 4162 977 1327 Fragments arranged by size: 4162 1327 977 AgeI A'CCGG_T Cuts at: 5297 5297 Size: 6466 AhdI GACnn_n'nnGTC Cuts at: 5195 5195 Size: 6466 AleI CACnn'nnGTG Cuts at: 1166 2912 3026 1166 Size: 1746 114 4606 Fragments arranged by size: 4606 1746 114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3620 1184 Size: 2436 4030 AlwNI CAG_nnn'CTG Cuts at: 2742 4209 5781 2742 Size: 1467 1572 3427 Fragments arranged by size: 3427 1572 1467 ApaLI G'TGCA_C Cuts at: 3400 4523 5679 3400 Size: 1123 1156 4187 Fragments arranged by size: 4187 1156 1123 ApoI r'AATT_y Cuts at: 1493 3775 4001 4153 6262 1493 Size: 2282 226 152 2109 1697 Fragments arranged by size: 2282 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5020 160 Size: 872 3988 1606 Fragments arranged by size: 3988 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 AvaI C'yCGr_G Cuts at: 1870 1943 2174 3791 1870 Size: 73 231 1617 4545 Fragments arranged by size: 4545 1617 231 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3222 3255 3222 Size: 33 6433 BamHI G'GATC_C Cuts at: 3796 4163 3796 Size: 367 6099 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2301 2494 2716 2754 2875 Size: 325 217 627 514 193 222 38 121 Cuts at: 2875 3012 3238 3787 5242 618 Size: 137 226 549 1455 1842 Fragments arranged by size: 1842 1455 627 549 514 325 226 222 217 193 137 121 38 BanII G_rGCy'C Cuts at: 1391 2391 2486 3074 3785 1391 Size: 1000 95 588 711 4072 Fragments arranged by size: 4072 1000 711 588 95 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2079 928 Size: 971 180 5315 Fragments arranged by size: 5315 971 180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3634 3668 4657 4691 1903 Size: 34 50 34 1613 34 989 34 3678 Fragments arranged by size: 3678 1613 989 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4358 5568 4358 Size: 1210 5256 BclI T'GATC_A Cuts at: 1548 3294 1548 Size: 1746 4720 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2031 3102 5077 Size: 107 122 71 631 605 359 1071 1975 Cuts at: 5077 136 Size: 1525 Fragments arranged by size: 1975 1525 1071 631 605 359 122 107 71 BglII A'GATC_T Cuts at: 6461 6461 Size: 6466 BlpI GC'TnA_GC Cuts at: 2480 3301 3632 3883 2480 Size: 821 331 251 5063 Fragments arranged by size: 5063 821 331 251 Bme1580I G_kGCm'C Cuts at: 1790 2499 2757 2805 2878 3404 4527 5683 1790 Size: 709 258 48 73 526 1123 1156 2573 Fragments arranged by size: 2573 1156 1123 709 526 258 73 48 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6466 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4197 5155 442 Size: 416 53 83 399 24 2780 958 1753 Fragments arranged by size: 2780 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6466 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2135 2420 2594 2956 3430 2135 Size: 285 174 362 474 5171 Fragments arranged by size: 5171 474 362 285 174 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2643 5126 1239 Size: 1404 2483 2579 Fragments arranged by size: 2579 2483 1404 Bpu10I CC'TnA_GC Cuts at: 3641 3641 Size: 6466 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3416 4591 5456 5754 3416 Size: 1175 865 298 4128 Fragments arranged by size: 4128 1175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2391 2661 2675 3401 5129 882 Size: 617 892 270 14 726 1728 2219 Fragments arranged by size: 2219 1728 892 726 617 270 14 BsaAI yAC'GTr Cuts at: 493 1204 3682 4257 493 Size: 711 2478 575 2702 Fragments arranged by size: 2702 2478 711 575 BsaBI GATnn'nnATC Cuts at: 6460 6460 Size: 6466 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4273 4655 5290 275 Size: 53 83 186 936 2740 382 635 1451 Fragments arranged by size: 2740 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4899 5297 5571 5718 6129 4899 Size: 398 274 147 411 5236 Fragments arranged by size: 5236 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2641 2671 1178 Size: 30 165 30 1238 30 4973 Fragments arranged by size: 4973 1238 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2270 3443 1186 Size: 1084 1173 4209 Fragments arranged by size: 4209 1173 1084 BseYI C'CCAG_C Cuts at: 2058 2813 3067 3309 3626 5669 6165 2058 Size: 755 254 242 317 2043 496 2359 Fragments arranged by size: 2359 2043 755 496 317 254 242 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1999 1163 Size: 573 263 5630 Fragments arranged by size: 5630 573 263 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3916 4677 5705 1540 Size: 275 186 1915 761 1028 2301 Fragments arranged by size: 2301 1915 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2361 2391 2937 3404 3720 4527 4612 5683 2361 Size: 30 546 467 316 807 85 1071 3144 Fragments arranged by size: 3144 1071 807 546 467 316 85 30 BsmI GAATG_Cn' Cuts at: 3977 4070 3977 Size: 93 6373 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2361 2391 2486 2499 2757 2805 2878 Size: 399 571 30 95 13 258 48 73 Cuts at: 2878 2937 3074 3404 3720 3785 4527 4612 5683 Size: 59 137 330 316 65 742 85 1071 Cuts at: 5683 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 571 399 330 316 258 137 95 85 73 65 59 48 30 13 BspEI T'CCGG_A Cuts at: 6129 6129 Size: 6466 BspHI T'CATG_A Cuts at: 4355 6085 4355 Size: 1730 4736 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2149 2903 3074 3612 3821 844 Size: 1305 754 171 538 209 3489 Fragments arranged by size: 3489 1305 754 538 209 171 BsrBI CCG'CTC Cuts at: 1394 1823 4353 1394 Size: 429 2530 3507 Fragments arranged by size: 3507 2530 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4961 5135 65 Size: 1183 3713 174 1396 Fragments arranged by size: 3713 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5110 5297 1700 Size: 110 3300 187 2869 Fragments arranged by size: 3300 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6466 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6466 BssSI C'ACGA_G Cuts at: 3745 4526 5538 3745 Size: 781 1012 4673 Fragments arranged by size: 4673 1012 781 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6466 BstBI TT'CG_AA Cuts at: 5270 5270 Size: 6466 BstEII G'GTnAC_C Cuts at: 1184 2179 1184 Size: 995 5471 BstXI CCAn_nnnn'nTGG Cuts at: 2506 2858 3278 3418 3622 2506 Size: 352 420 140 204 5350 Fragments arranged by size: 5350 420 352 204 140 BstYI r'GATC_y Cuts at: 1972 2514 2572 3796 4163 4548 4565 6017 6103 Size: 542 58 1224 367 385 17 1452 86 Cuts at: 6103 6115 6461 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1224 542 385 367 346 86 58 17 12 Bsu36I CC'TnA_GG Cuts at: 2880 2880 Size: 6466 BtgI C'CryG_G Cuts at: 513 1065 2271 3271 3576 513 Size: 552 1206 1000 305 3403 Fragments arranged by size: 3403 1206 1000 552 305 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5727 Fragments arranged by size: 5727 683 56 BtsI GCAGTG_nn' Cuts at: 2436 2782 3350 3982 4776 4796 2436 Size: 346 568 632 794 20 4106 Fragments arranged by size: 4106 794 632 568 346 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6466 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6431 DraI TTT'AAA Cuts at: 3770 3770 Size: 6466 DraIII CAC_nnn'GTG Cuts at: 2524 2524 Size: 6466 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5473 809 Size: 507 4157 1802 Fragments arranged by size: 4157 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3579 3612 3913 Size: 54 1425 50 275 18 1749 33 301 Cuts at: 3913 4802 6431 8 Size: 889 1629 43 Fragments arranged by size: 1749 1629 1425 889 301 275 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3913 1537 Size: 275 2101 4090 Fragments arranged by size: 4090 2101 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2234 2862 3548 4396 1353 Size: 881 628 686 848 3423 Fragments arranged by size: 3423 881 848 686 628 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5053 5427 5573 387 Size: 1064 138 3464 374 146 1280 Fragments arranged by size: 3464 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2389 2389 Size: 6466 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2643 3517 4529 5126 5913 6181 1239 Size: 393 1011 874 1012 597 787 268 1524 Fragments arranged by size: 1524 1012 1011 874 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2231 3488 2231 Size: 1257 5209 EcoO109I rG'GnC_Cy Cuts at: 2817 3148 2817 Size: 331 6135 EcoRI G'AATT_C Cuts at: 3775 4153 3775 Size: 378 6088 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2417 2644 2417 Size: 227 6239 FspI TGC'GCA Cuts at: 4972 4972 Size: 6466 HaeII r_GCGC'y Cuts at: 1678 3376 3754 5613 1678 Size: 1698 378 1859 2531 Fragments arranged by size: 2531 1859 1698 378 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5107 5139 5181 5213 1373 Size: 32 135 32 3535 32 42 32 2626 Fragments arranged by size: 3535 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3810 4056 669 Size: 988 281 1872 246 3079 Fragments arranged by size: 3079 1872 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6466 HpaI GTT'AAC Cuts at: 4056 4056 Size: 6466 KpnI G_GTAC'C Cuts at: 2305 3242 3791 2305 Size: 937 549 4980 Fragments arranged by size: 4980 937 549 MfeI C'AATT_G Cuts at: 4065 4065 Size: 6466 MluI A'CGCG_T Cuts at: 6342 6342 Size: 6466 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3229 5580 5764 1305 Size: 1924 2351 184 2007 Fragments arranged by size: 2351 2007 1924 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4644 Fragments arranged by size: 4644 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2912 3026 4424 4783 4942 518 Size: 648 1746 114 1398 359 159 2042 Fragments arranged by size: 2042 1746 1398 648 359 159 114 MspA1I CmG'CkG Cuts at: 1606 2308 3305 3313 3648 3882 4559 5707 5952 Size: 702 997 8 335 234 677 1148 245 Cuts at: 5952 1606 Size: 2120 Fragments arranged by size: 2120 1148 997 702 677 335 245 234 8 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5914 NdeI CA'TA_TG Cuts at: 387 387 Size: 6466 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6466 NotI GC'GGCC_GC Cuts at: 3913 3913 Size: 6466 NspI r_CATG'y Cuts at: 1132 1229 3824 5369 1132 Size: 97 2595 1545 2229 Fragments arranged by size: 2595 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2296 3418 1348 Size: 948 1122 4396 Fragments arranged by size: 4396 1122 948 PciI A'CATG_T Cuts at: 5365 5365 Size: 6466 PflMI CCAn_nnn'nTGG Cuts at: 1108 2764 1108 Size: 1656 4810 PfoI T'CCnGG_A Cuts at: 3557 3557 Size: 6466 PmeI GTTT'AAAC Cuts at: 3770 3770 Size: 6466 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4535 4567 6048 6080 4535 Size: 32 1481 32 4921 Fragments arranged by size: 4921 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2817 2817 Size: 6466 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6466 PsiI TTA'TAA Cuts at: 4036 4036 Size: 6466 PstI C_TGCA'G Cuts at: 830 2040 2160 3479 3818 830 Size: 1210 120 1319 339 3478 Fragments arranged by size: 3478 1319 1210 339 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6466 PvuII CAG'CTG Cuts at: 2308 3313 2308 Size: 1005 5461 SacI G_AGCT'C Cuts at: 2391 2391 Size: 6466 SalI G'TCGA_C Cuts at: 1936 3808 1936 Size: 1872 4594 SapI GCTCTTCn'nnn_ Cuts at: 3548 3548 Size: 6466 SbfI CC_TGCA'GG Cuts at: 3818 3818 Size: 6466 ScaI AGT'ACT Cuts at: 4714 6426 4714 Size: 1712 4754 SexAI A'CCwGG_T Cuts at: 2712 2712 Size: 6466 SfcI C'TryA_G Cuts at: 826 1045 2036 2156 2842 3475 3814 4949 5630 Size: 219 991 120 686 633 339 1135 681 Cuts at: 5630 5821 826 Size: 191 1471 Fragments arranged by size: 1471 1135 991 686 681 633 339 219 191 120 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6466 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6466 SmaI CCC'GGG Cuts at: 1872 3793 1872 Size: 1921 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3395 4570 5471 5733 820 Size: 197 926 1452 1175 901 262 1553 Fragments arranged by size: 1553 1452 1175 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4257 493 Size: 3764 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6466 SphI G_CATG'C Cuts at: 1132 3824 1132 Size: 2692 3774 SspI AAT'ATT Cuts at: 5 52 4390 5 Size: 47 4338 2081 Fragments arranged by size: 4338 2081 47 StuI AGG'CCT Cuts at: 1517 2130 3127 3728 1517 Size: 613 997 601 4255 Fragments arranged by size: 4255 997 613 601 StyI C'CwwG_G Cuts at: 513 1065 2392 3160 3234 3905 513 Size: 552 1327 768 74 671 3074 Fragments arranged by size: 3074 1327 768 671 552 74 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2935 4493 4510 4663 4848 5293 325 Size: 1255 1355 1558 17 153 185 445 1498 Fragments arranged by size: 1558 1498 1355 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4712 6424 96 Size: 274 80 33 51 1443 2735 1712 138 Fragments arranged by size: 2735 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2641 2673 6426 6458 2641 Size: 32 3753 32 2649 Fragments arranged by size: 3753 2649 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6466 XbaI T'CTAG_A Cuts at: 3802 3802 Size: 6466 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2293 2293 Size: 6466 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6466 XmaI C'CCGG_G Cuts at: 1870 3791 1870 Size: 1921 4545 XmnI GAAnn'nnTTC Cuts at: 4595 4595 Size: 6466 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4274 276 Size: 53 83 186 936 2740 2468 Fragments arranged by size: 2740 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI