################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## Acc65I AclI AflIII AhdI Alw44I AlwNI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BcgI BclI BlnI BlpI Bpu1102I BsaBI BsiHKAI BsiWI BsmBI BspDI BspHI BspLU11I BsrDI BssHII BssSI BstBI BstEII BstPI CelII ClaI CpoI DrdI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HindIII KpnI KspI MamI MfeI MluI MunI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 1756 GACGTC GACGT^C AccI 504 GTATAC GT^[AC][GT]AC AccIII 1670 TCCGGA T^CCGGA AflII 1948 CTTAAG C^TTAAG AgeI 342 ACCGGT A^CCGGT Aor51HI 1183 AGCGCT AGC^GCT Asp700I 1063 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 438 TGGCCA TGG^CCA BamHI 916 GGATCC G^GATCC BanI 137 GGCGCC G^G[CT][GA]CC BbeI 137 GGCGCC GGCGC^C BbrPI 1288 CACGTG CAC^GTG BbvCI 84 CCTCAGC CCTCAGC(-5/-2) BfrI 1948 CTTAAG C^TTAAG BglII 1560 AGATCT A^GATCT BsaAI 1288 CACGTG [CT]AC^GT[GA] BsaI 228 GAGACC GGTCTC(1/5) BseAI 1670 TCCGGA T^CCGGA BsgI 445 GTGCAG GTGCAG(16/14) BsmI 1510 GCATTC GAATGC(1/-1) Bsp1407I 1367 TGTACA T^GTACA BspEI 1670 TCCGGA T^CCGGA BspMI 447 GCAGGT ACCTGC(4/8) BsrBI 1302 CCGCTC CCGCTC(-3/-3) BsrGI 1367 TGTACA T^GTACA Bst1107I 504 GTATAC GTA^TAC BstAPI 1414 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 504 GTATAC GTA^TAC DraI 2005 TTTAAA TTT^AAA DraIII 1937 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 353 CCGTGG C^C[GA][CT]GG Eco47III 1183 AGCGCT AGC^GCT EcoT14I 471 CCAAGG C^C[AT][AT]GG HpaI 828 GTTAAC GTT^AAC KasI 137 GGCGCC G^GCGCC MluNI 438 TGGCCA TGG^CCA MroI 1670 TCCGGA T^CCGGA MscI 438 TGGCCA TGG^CCA NarI 137 GGCGCC GG^CGCC PaeR7I 312 CTCGAG C^TCGAG PinAI 342 ACCGGT A^CCGGT PmaCI 1288 CACGTG CAC^GTG PmlI 1288 CACGTG CAC^GTG PpuMI 1646 AGGTCCC [GA]G^G[AT]CC[CT] PstI 1591 CTGCAG CTGCA^G SapI 1888 GAAGAGC GCTCTTC(1/4) SfoI 137 GGCGCC GGC^GCC SgrAI 341 CACCGGTG C[GA]^CCGG[CT]G SspBI 1367 TGTACA T^GTACA StuI 819 AGGCCT AGG^CCT StyI 471 CCAAGG C^C[AT][AT]GG TfiI 304 GAATC G^A[AT]TC XhoI 312 CTCGAG C^TCGAG XmnI 1063 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 137 GGCGCC G[GA]^CG[CT]C 1756 GACGTC AocI 1092 CCTGAGG CC^T[ACGT]AGG 2047 CCTGAGG ApaI 357 GGGCCC GGGCC^C 1777 GGGCCC BbsI 2078 GAAGAC GAAGAC(2/6) 977 GTCTTC BciVI 1765 GGATAC GTATCC(6/5) 524 GTATCC BglI 163 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 431 GCCTCTATGGC BpmI 183 CTGGAG CTGGAG(16/14) 2015 CTGGAG BpuAI 2078 GAAGAC GAAGAC(2/6) 977 GTCTTC BsaHI 137 GGCGCC G[GA]^CG[CT]C 1756 GACGTC BsaWI 342 ACCGGT [AT]^CCGG[AT] 1670 TCCGGA BsmAI 228 GAGAC GTCTC(1/5) 1278 GTCTC BstXI 921 CCAGGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1283 CCAGCCACGTGG Bsu36I 1092 CCTGAGG CC^T[ACGT]AGG 2047 CCTGAGG DraII 357 GGGCCCC [GA]G^G[ACGT]CC[CT] 1646 AGGTCCC EagI 170 CGGCCG C^GGCCG 363 CGGCCG EclXI 170 CGGCCG C^GGCCG 363 CGGCCG Eco52I 170 CGGCCG C^GGCCG 363 CGGCCG Eco81I 1092 CCTGAGG CC^T[ACGT]AGG 2047 CCTGAGG EcoO109I 357 GGGCCCC [GA]G^G[ACGT]CC[CT] 1646 AGGTCCC HaeII 137 GGCGCC [GA]GCGC^[CT] 1183 AGCGCT Hin1I 137 GGCGCC G[GA]^CG[CT]C 1756 GACGTC HphI 1089 TCACC GGTGA(8/7) 1871 TCACC MspA1I 1588 CAGCTG C[AC]G^C[GT]G 1725 CAGCTG PvuII 1588 CAGCTG CAG^CTG 1725 CAGCTG SfcI 1591 CTGCAG C^T[GA][CT]AG 1830 CTGTAG SspI 770 AATATT AAT^ATT 1054 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AvaI AvaII BanII BbvI BcnI BfaI BmyI BsaJI BseRI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII