(Circular) (Six-base) MAPSORT of: pFN21AE5740.seq Check: 9277 from: 1 to: 6811 pFN21AE5740 6811 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3765 4621 278 Size: 53 83 186 936 2229 856 2468 Fragments arranged by size: 2468 2229 936 856 186 83 53 AccI GT'mk_AC Cuts at: 1937 2510 4154 1937 Size: 573 1644 4594 Fragments arranged by size: 4594 1644 573 Acc65I G'GTAC_C Cuts at: 4132 4132 Size: 6811 AclI AA'CG_TT Cuts at: 1563 4938 5311 1563 Size: 3375 373 3063 Fragments arranged by size: 3375 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2813 4874 6258 6526 1632 Size: 1181 2061 1384 268 1917 Fragments arranged by size: 2061 1917 1384 1181 268 AfeI AGC'GCT Cuts at: 3190 3190 Size: 6811 AflII C'TTAA_G Cuts at: 820 1017 3953 820 Size: 197 2936 3678 Fragments arranged by size: 3678 2936 197 AflIII A'CryG_T Cuts at: 1203 5710 6687 1203 Size: 4507 977 1327 Fragments arranged by size: 4507 1327 977 AgeI A'CCGG_T Cuts at: 2347 5642 2347 Size: 3295 3516 AhdI GACnn_n'nnGTC Cuts at: 5540 5540 Size: 6811 AleI CACnn'nnGTG Cuts at: 1166 3881 1166 Size: 2715 4096 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3068 1184 Size: 1884 4927 AlwNI CAG_nnn'CTG Cuts at: 4554 6126 4554 Size: 1572 5239 ApaI G_GGCC'C Cuts at: 2366 3786 2366 Size: 1420 5391 ApaLI G'TGCA_C Cuts at: 4868 6024 4868 Size: 1156 5655 ApoI r'AATT_y Cuts at: 1493 2593 2644 3026 3440 3555 4120 4346 4498 Size: 1100 51 382 414 115 565 226 152 Cuts at: 4498 6607 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1100 565 414 382 226 152 115 51 AseI AT'TA_AT Cuts at: 160 1032 5365 160 Size: 872 4333 1606 Fragments arranged by size: 4333 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6811 AvaI C'yCGr_G Cuts at: 1870 1943 2014 2114 2288 2317 4136 1870 Size: 73 71 100 174 29 1819 4545 Fragments arranged by size: 4545 1819 174 100 73 71 29 BamHI G'GATC_C Cuts at: 2921 4141 4508 2921 Size: 1220 367 5224 Fragments arranged by size: 5224 1220 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2142 4132 5587 618 Size: 325 217 627 355 1990 1455 1842 Fragments arranged by size: 1990 1842 1455 627 355 325 217 BanII G_rGCy'C Cuts at: 1391 2203 2366 3252 3786 4130 1391 Size: 812 163 886 534 344 4072 Fragments arranged by size: 4072 886 812 534 344 163 BbeI G_GCGC'C Cuts at: 2146 2146 Size: 6811 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2975 4090 928 Size: 971 1076 1115 3649 Fragments arranged by size: 3649 1115 1076 971 BbvCI CC'TCA_GC Cuts at: 2090 2090 Size: 6811 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5002 5036 1903 Size: 34 3065 34 3678 Fragments arranged by size: 3678 3065 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2540 3764 4703 5913 2540 Size: 1224 939 1210 3438 Fragments arranged by size: 3438 1224 1210 939 BclI T'GATC_A Cuts at: 1548 1548 Size: 6811 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2174 2442 5422 Size: 107 122 71 631 605 502 268 2980 Cuts at: 5422 136 Size: 1525 Fragments arranged by size: 2980 1525 631 605 502 268 122 107 71 BglII A'GATC_T Cuts at: 3565 6806 3565 Size: 3241 3570 BlpI GC'TnA_GC Cuts at: 4228 4228 Size: 6811 Bme1580I G_kGCm'C Cuts at: 1790 2366 3786 3888 4872 6028 1790 Size: 576 1420 102 984 1156 2573 Fragments arranged by size: 2573 1420 1156 984 576 102 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6811 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4542 5500 442 Size: 416 53 83 399 24 3125 958 1753 Fragments arranged by size: 3125 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6811 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2772 2772 Size: 6811 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2209 4041 5471 1239 Size: 970 1832 1430 2579 Fragments arranged by size: 2579 1832 1430 970 Bpu10I CC'TnA_GC Cuts at: 2090 3587 3899 2090 Size: 1497 312 5002 Fragments arranged by size: 5002 1497 312 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2545 2806 3598 4936 5801 6099 2545 Size: 261 792 1338 865 298 3257 Fragments arranged by size: 3257 1338 865 792 298 261 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2227 5474 882 Size: 617 728 3247 2219 Fragments arranged by size: 3247 2219 728 617 BsaAI yAC'GTr Cuts at: 493 1204 3295 4602 493 Size: 711 2091 1307 2702 Fragments arranged by size: 2702 2091 1307 711 BsaBI GATnn'nnATC Cuts at: 6805 6805 Size: 6811 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2143 3762 4618 5000 Size: 53 83 186 936 610 1619 856 382 Cuts at: 5000 5635 275 Size: 635 1451 Fragments arranged by size: 1619 1451 936 856 635 610 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2347 3675 5244 5642 5916 6063 6474 2347 Size: 1328 1569 398 274 147 411 2684 Fragments arranged by size: 2684 1569 1328 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6586 Fragments arranged by size: 6586 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2327 2643 4070 1186 Size: 1141 316 1427 3927 Fragments arranged by size: 3927 1427 1141 316 BseYI C'CCAG_C Cuts at: 2079 2252 3287 3732 6014 6510 2079 Size: 173 1035 445 2282 496 2380 Fragments arranged by size: 2380 2282 1035 496 445 173 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2471 1163 Size: 573 735 5503 Fragments arranged by size: 5503 735 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2137 2178 2371 2394 4261 5022 Size: 275 186 136 41 193 23 1867 761 Cuts at: 5022 6050 1540 Size: 1028 2301 Fragments arranged by size: 2301 1867 1028 761 275 193 186 136 41 23 BsiHKAI G_wGCw'C Cuts at: 4872 4957 6028 4872 Size: 85 1071 5655 Fragments arranged by size: 5655 1071 85 BsmI GAATG_Cn' Cuts at: 3515 4322 4415 3515 Size: 807 93 5911 Fragments arranged by size: 5911 807 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2203 2366 3252 3786 3888 4130 4872 Size: 399 413 163 886 534 102 242 742 Cuts at: 4872 4957 6028 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 886 742 534 413 399 242 163 102 85 BspEI T'CCGG_A Cuts at: 3675 6474 3675 Size: 2799 4012 BspHI T'CATG_A Cuts at: 4700 6430 4700 Size: 1730 5081 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2443 4166 844 Size: 1599 1723 3489 Fragments arranged by size: 3489 1723 1599 BsrBI CCG'CTC Cuts at: 1394 1823 3309 4698 1394 Size: 429 1486 1389 3507 Fragments arranged by size: 3507 1486 1389 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5306 5480 65 Size: 1183 4058 174 1396 Fragments arranged by size: 4058 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2097 2109 2177 2328 2347 2389 5455 Size: 110 287 12 68 151 19 42 3066 Cuts at: 5455 5642 1700 Size: 187 2869 Fragments arranged by size: 3066 2869 287 187 151 110 68 42 19 12 BsrGI T'GTAC_A Cuts at: 96 3372 96 Size: 3276 3535 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6811 BssSI C'ACGA_G Cuts at: 4871 5883 4871 Size: 1012 5799 BstAPI GCAn_nnn'nTGC Cuts at: 794 3425 794 Size: 2631 4180 BstBI TT'CG_AA Cuts at: 5615 5615 Size: 6811 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6811 BstXI CCAn_nnnn'nTGG Cuts at: 2933 3295 2933 Size: 362 6449 BstYI r'GATC_y Cuts at: 1972 2418 2921 3565 3750 4141 4508 4893 4910 Size: 446 503 644 185 391 367 385 17 Cuts at: 4910 6362 6448 6460 6806 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 644 503 446 391 385 367 346 185 86 17 12 BstZ17I GTA'TAC Cuts at: 2511 2511 Size: 6811 Bsu36I CC'TnA_GG Cuts at: 3098 4053 3098 Size: 955 5856 BtgI C'CryG_G Cuts at: 513 1065 2004 2358 513 Size: 552 939 354 4966 Fragments arranged by size: 4966 939 552 354 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6072 Fragments arranged by size: 6072 683 56 BtsI GCAGTG_nn' Cuts at: 4327 5121 5141 4327 Size: 794 20 5997 Fragments arranged by size: 5997 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6811 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6776 DraI TTT'AAA Cuts at: 4012 4115 4012 Size: 103 6708 DraIII CAC_nnn'GTG Cuts at: 3947 3947 Size: 6811 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5818 809 Size: 507 4502 1802 Fragments arranged by size: 4502 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2368 2443 Size: 54 1425 50 275 18 345 193 75 Cuts at: 2443 4258 5147 6776 8 Size: 1815 889 1629 43 Fragments arranged by size: 1815 1629 1425 889 345 275 193 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2175 2368 4258 1537 Size: 275 363 193 1890 4090 Fragments arranged by size: 4090 1890 363 275 193 EarI CTCTTCn'nnn_ Cuts at: 1353 2800 2856 3888 4741 1353 Size: 1447 56 1032 853 3423 Fragments arranged by size: 3423 1447 1032 853 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2302 5398 5772 5918 387 Size: 1064 138 435 278 3096 374 146 1280 Fragments arranged by size: 3096 1280 1064 435 374 278 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2209 2813 4041 4874 5471 6258 6526 Size: 393 577 604 1228 833 597 787 268 Cuts at: 6526 1239 Size: 1524 Fragments arranged by size: 1524 1228 833 787 604 597 577 393 268 EcoO109I rG'GnC_Cy Cuts at: 2363 3652 2363 Size: 1289 5522 EcoRI G'AATT_C Cuts at: 4120 4498 4120 Size: 378 6433 FspI TGC'GCA Cuts at: 5317 5317 Size: 6811 HaeII r_GCGC'y Cuts at: 1678 2146 3192 5958 1678 Size: 468 1046 2766 2531 Fragments arranged by size: 2766 2531 1046 468 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2486 2518 3385 3417 3631 Size: 32 135 32 914 32 867 32 214 Cuts at: 3631 3663 5452 5484 5526 5558 1373 Size: 32 1789 32 42 32 2626 Fragments arranged by size: 2626 1789 914 867 214 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2835 2943 2995 3220 4155 4401 Size: 988 281 897 108 52 225 935 246 Cuts at: 4401 669 Size: 3079 Fragments arranged by size: 3079 988 935 897 281 246 225 108 52 HindIII A'AGCT_T Cuts at: 748 748 Size: 6811 HpaI GTT'AAC Cuts at: 2835 4401 2835 Size: 1566 5245 KasI G'GCGC_C Cuts at: 2142 2142 Size: 6811 KpnI G_GTAC'C Cuts at: 4136 4136 Size: 6811 MfeI C'AATT_G Cuts at: 4410 4410 Size: 6811 MluI A'CGCG_T Cuts at: 6687 6687 Size: 6811 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2777 2914 3904 5925 6109 1305 Size: 1472 137 990 2021 184 2007 Fragments arranged by size: 2021 2007 1472 990 184 137 MscI TGG'CCA Cuts at: 10 64 1489 1832 2445 10 Size: 54 1425 343 613 4376 Fragments arranged by size: 4376 1425 613 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 3690 3848 3881 4769 5128 5287 Size: 648 1572 952 158 33 888 359 159 Cuts at: 5287 518 Size: 2042 Fragments arranged by size: 2042 1572 952 888 648 359 159 158 33 MspA1I CmG'CkG Cuts at: 1606 3595 3732 4227 4904 6052 6297 1606 Size: 1989 137 495 677 1148 245 2120 Fragments arranged by size: 2120 1989 1148 677 495 245 137 NaeI GCC'GGC Cuts at: 2099 2111 2179 2099 Size: 12 68 6731 Fragments arranged by size: 6731 68 12 NarI GG'CG_CC Cuts at: 2143 2143 Size: 6811 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5320 Fragments arranged by size: 5320 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6811 NgoMIV G'CCGG_C Cuts at: 2097 2109 2177 2097 Size: 12 68 6731 Fragments arranged by size: 6731 68 12 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6811 NotI GC'GGCC_GC Cuts at: 4258 4258 Size: 6811 NspI r_CATG'y Cuts at: 1132 1229 4169 5714 1132 Size: 97 2940 1545 2229 Fragments arranged by size: 2940 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6811 PciI A'CATG_T Cuts at: 5710 5710 Size: 6811 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6811 PfoI T'CCnGG_A Cuts at: 3753 4018 3753 Size: 265 6546 PmeI GTTT'AAAC Cuts at: 4115 4115 Size: 6811 PmlI CAC'GTG Cuts at: 3295 3295 Size: 6811 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2289 2321 4880 4912 6393 6425 2289 Size: 32 2559 32 1481 32 2675 Fragments arranged by size: 2675 2559 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3652 3652 Size: 6811 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6811 PsiI TTA'TAA Cuts at: 4381 4381 Size: 6811 PspOMI G'GGCC_C Cuts at: 2362 3782 2362 Size: 1420 5391 PspXI vC'TCGA_Gb Cuts at: 2317 2317 Size: 6811 PstI C_TGCA'G Cuts at: 830 3600 4163 830 Size: 2770 563 3478 Fragments arranged by size: 3478 2770 563 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6811 PvuII CAG'CTG Cuts at: 3595 3732 3595 Size: 137 6674 SalI G'TCGA_C Cuts at: 1936 4153 1936 Size: 2217 4594 SapI GCTCTTCn'nnn_ Cuts at: 3888 3888 Size: 6811 SbfI CC_TGCA'GG Cuts at: 4163 4163 Size: 6811 ScaI AGT'ACT Cuts at: 5059 6771 5059 Size: 1712 5099 SfcI C'TryA_G Cuts at: 826 1045 3596 3835 4159 5294 5975 6166 826 Size: 219 2551 239 324 1135 681 191 1471 Fragments arranged by size: 2551 1471 1135 681 324 239 219 191 SfoI GGC'GCC Cuts at: 2144 2144 Size: 6811 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6811 SgrAI Cr'CCGG_yG Cuts at: 1700 2347 1700 Size: 647 6164 SmaI CCC'GGG Cuts at: 1872 4138 1872 Size: 2266 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2317 2524 2785 3577 3953 4915 Size: 197 926 374 207 261 792 376 962 Cuts at: 4915 5816 6078 820 Size: 901 262 1553 Fragments arranged by size: 1553 962 926 901 792 376 374 262 261 207 197 SnaBI TAC'GTA Cuts at: 493 4602 493 Size: 4109 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6811 SphI G_CATG'C Cuts at: 1132 4169 1132 Size: 3037 3774 SspI AAT'ATT Cuts at: 5 52 2777 3061 4735 5 Size: 47 2725 284 1674 2081 Fragments arranged by size: 2725 2081 1674 284 47 StuI AGG'CCT Cuts at: 1517 2826 1517 Size: 1309 5502 StyI C'CwwG_G Cuts at: 513 1065 2004 2476 4250 513 Size: 552 939 472 1774 3074 Fragments arranged by size: 3074 1774 939 552 472 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4838 4855 5008 5193 5638 325 Size: 1255 3258 17 153 185 445 1498 Fragments arranged by size: 3258 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3372 5057 6769 Size: 274 80 33 51 1443 1395 1685 1712 Cuts at: 6769 96 Size: 138 Fragments arranged by size: 1712 1685 1443 1395 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2410 2442 3933 3965 6771 6803 2410 Size: 32 1491 32 2806 32 2418 Fragments arranged by size: 2806 2418 1491 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6811 XbaI T'CTAG_A Cuts at: 4147 4147 Size: 6811 XhoI C'TCGA_G Cuts at: 1943 2317 1943 Size: 374 6437 XmaI C'CCGG_G Cuts at: 1870 4136 1870 Size: 2266 4545 XmnI GAAnn'nnTTC Cuts at: 3072 4940 3072 Size: 1868 4943 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3763 4619 276 Size: 53 83 186 936 2229 856 2468 Fragments arranged by size: 2468 2229 936 856 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI