(Linear) (Six-base) MAPSORT of: KIEE5740.seq Check: 4392 from: 1 to: 2106 KIEE5740.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AatII G_ACGT'C Cuts at: 0 1760 2106 Size: 1760 346 AccI GT'mk_AC Cuts at: 0 505 2106 Size: 505 1601 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 808 2106 Size: 808 1298 AfeI AGC'GCT Cuts at: 0 1185 2106 Size: 1185 921 AflII C'TTAA_G Cuts at: 0 1948 2106 Size: 1948 158 AgeI A'CCGG_T Cuts at: 0 342 2106 Size: 342 1764 AleI CACnn'nnGTG Cuts at: 0 1876 2106 Size: 1876 230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1063 2106 Size: 1063 1043 ApaI G_GGCC'C Cuts at: 0 361 1781 2106 Size: 361 1420 325 Fragments arranged by size: 1420 361 325 ApoI r'AATT_y Cuts at: 0 588 639 1021 1435 1550 2106 Size: 588 51 382 414 115 556 Fragments arranged by size: 588 556 414 382 115 51 AvaI C'yCGr_G Cuts at: 0 9 109 283 312 2106 Size: 9 100 174 29 1794 Fragments arranged by size: 1794 174 100 29 9 BamHI G'GATC_C Cuts at: 0 916 2106 Size: 916 1190 BanI G'GyrC_C Cuts at: 0 137 2106 Size: 137 1969 BanII G_rGCy'C Cuts at: 0 198 361 1247 1781 2106 Size: 198 163 886 534 325 Fragments arranged by size: 886 534 325 198 163 BbeI G_GCGC'C Cuts at: 0 141 2106 Size: 141 1965 BbsI GAAGACnn'nnnn_ Cuts at: 0 970 2085 2106 Size: 970 1115 21 Fragments arranged by size: 1115 970 21 BbvCI CC'TCA_GC Cuts at: 0 85 2106 Size: 85 2021 BciVI GTATCCnnnnn_n' Cuts at: 0 535 1759 2106 Size: 535 1224 347 Fragments arranged by size: 1224 535 347 BglI GCCn_nnn'nGGC Cuts at: 0 169 437 2106 Size: 169 268 1669 Fragments arranged by size: 1669 268 169 BglII A'GATC_T Cuts at: 0 1560 2106 Size: 1560 546 Bme1580I G_kGCm'C Cuts at: 0 361 1781 1883 2106 Size: 361 1420 102 223 Fragments arranged by size: 1420 361 223 102 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 767 2106 Size: 767 1339 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 204 2036 2106 Size: 204 1832 70 Fragments arranged by size: 1832 204 70 Bpu10I CC'TnA_GC Cuts at: 0 85 1582 1894 2106 Size: 85 1497 312 212 Fragments arranged by size: 1497 312 212 85 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 540 801 1593 2106 Size: 540 261 792 513 Fragments arranged by size: 792 540 513 261 BsaI GGTCTCn'nnnn_ Cuts at: 0 222 2106 Size: 222 1884 BsaAI yAC'GTr Cuts at: 0 1290 2106 Size: 1290 816 BsaHI Gr'CG_yC Cuts at: 0 138 1757 2106 Size: 138 1619 349 Fragments arranged by size: 1619 349 138 BsaWI w'CCGG_w Cuts at: 0 342 1670 2106 Size: 342 1328 436 Fragments arranged by size: 1328 436 342 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 322 638 2065 2106 Size: 322 316 1427 41 Fragments arranged by size: 1427 322 316 41 BseYI C'CCAG_C Cuts at: 0 74 247 1282 1727 2106 Size: 74 173 1035 445 379 Fragments arranged by size: 1035 445 379 173 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 466 2106 Size: 466 1640 BsiEI CG_ry'CG Cuts at: 0 132 173 366 389 2106 Size: 132 41 193 23 1717 Fragments arranged by size: 1717 193 132 41 23 BsmI GAATG_Cn' Cuts at: 0 1510 2106 Size: 1510 596 Bsp1286I G_dGCh'C Cuts at: 0 198 361 1247 1781 1883 2106 Size: 198 163 886 534 102 223 Fragments arranged by size: 886 534 223 198 163 102 BspEI T'CCGG_A Cuts at: 0 1670 2106 Size: 1670 436 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 438 2106 Size: 438 1668 BsrBI CCG'CTC Cuts at: 0 1304 2106 Size: 1304 802 BsrFI r'CCGG_y Cuts at: 0 92 104 172 323 342 384 2106 Size: 92 12 68 151 19 42 1722 Fragments arranged by size: 1722 151 92 68 42 19 12 BsrGI T'GTAC_A Cuts at: 0 1367 2106 Size: 1367 739 BstAPI GCAn_nnn'nTGC Cuts at: 0 1420 2106 Size: 1420 686 BstXI CCAn_nnnn'nTGG Cuts at: 0 928 1290 2106 Size: 928 362 816 Fragments arranged by size: 928 816 362 BstYI r'GATC_y Cuts at: 0 413 916 1560 1745 2106 Size: 413 503 644 185 361 Fragments arranged by size: 644 503 413 361 185 BstZ17I GTA'TAC Cuts at: 0 506 2106 Size: 506 1600 Bsu36I CC'TnA_GG Cuts at: 0 1093 2048 2106 Size: 1093 955 58 Fragments arranged by size: 1093 955 58 BtgI C'CryG_G Cuts at: 0 353 2106 Size: 353 1753 DraI TTT'AAA Cuts at: 0 2007 2106 Size: 2007 99 DraIII CAC_nnn'GTG Cuts at: 0 1942 2106 Size: 1942 164 EaeI y'GGCC_r Cuts at: 0 170 363 438 2106 Size: 170 193 75 1668 Fragments arranged by size: 1668 193 170 75 EagI C'GGCC_G Cuts at: 0 170 363 2106 Size: 170 193 1743 Fragments arranged by size: 1743 193 170 EarI CTCTTCn'nnn_ Cuts at: 0 795 851 1883 2106 Size: 795 56 1032 223 Fragments arranged by size: 1032 795 223 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 297 2106 Size: 19 278 1809 Fragments arranged by size: 1809 278 19 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 204 808 2036 2106 Size: 204 604 1228 70 Fragments arranged by size: 1228 604 204 70 EcoO109I rG'GnC_Cy Cuts at: 0 358 1647 2106 Size: 358 1289 459 Fragments arranged by size: 1289 459 358 HaeII r_GCGC'y Cuts at: 0 141 1187 2106 Size: 141 1046 919 Fragments arranged by size: 1046 919 141 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 481 513 1380 1412 1626 1658 2106 Size: 481 32 867 32 214 32 448 Fragments arranged by size: 867 481 448 214 32 32 32 HincII GTy'rAC Cuts at: 0 830 938 990 1215 2106 Size: 830 108 52 225 891 Fragments arranged by size: 891 830 225 108 52 HpaI GTT'AAC Cuts at: 0 830 2106 Size: 830 1276 KasI G'GCGC_C Cuts at: 0 137 2106 Size: 137 1969 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 772 909 1899 2106 Size: 772 137 990 207 Fragments arranged by size: 990 772 207 137 MscI TGG'CCA Cuts at: 0 440 2106 Size: 440 1666 MslI CAynn'nnrTG Cuts at: 0 733 1685 1843 1876 2106 Size: 733 952 158 33 230 Fragments arranged by size: 952 733 230 158 33 MspA1I CmG'CkG Cuts at: 0 1590 1727 2106 Size: 1590 137 379 Fragments arranged by size: 1590 379 137 NaeI GCC'GGC Cuts at: 0 94 106 174 2106 Size: 94 12 68 1932 Fragments arranged by size: 1932 94 68 12 NarI GG'CG_CC Cuts at: 0 138 2106 Size: 138 1968 NgoMIV G'CCGG_C Cuts at: 0 92 104 172 2106 Size: 92 12 68 1934 Fragments arranged by size: 1934 92 68 12 PfoI T'CCnGG_A Cuts at: 0 1748 2013 2106 Size: 1748 265 93 Fragments arranged by size: 1748 265 93 PmlI CAC'GTG Cuts at: 0 1290 2106 Size: 1290 816 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 284 316 2106 Size: 284 32 1790 Fragments arranged by size: 1790 284 32 PpuMI rG'GwC_Cy Cuts at: 0 1647 2106 Size: 1647 459 PspOMI G'GGCC_C Cuts at: 0 357 1777 2106 Size: 357 1420 329 Fragments arranged by size: 1420 357 329 PspXI vC'TCGA_Gb Cuts at: 0 312 2106 Size: 312 1794 PstI C_TGCA'G Cuts at: 0 1595 2106 Size: 1595 511 PvuII CAG'CTG Cuts at: 0 1590 1727 2106 Size: 1590 137 379 Fragments arranged by size: 1590 379 137 SapI GCTCTTCn'nnn_ Cuts at: 0 1883 2106 Size: 1883 223 SfcI C'TryA_G Cuts at: 0 1591 1830 2106 Size: 1591 239 276 Fragments arranged by size: 1591 276 239 SfoI GGC'GCC Cuts at: 0 139 2106 Size: 139 1967 SgrAI Cr'CCGG_yG Cuts at: 0 342 2106 Size: 342 1764 SmlI C'TyrA_G Cuts at: 0 312 519 780 1572 1948 2106 Size: 312 207 261 792 376 158 Fragments arranged by size: 792 376 312 261 207 158 SspI AAT'ATT Cuts at: 0 772 1056 2106 Size: 772 284 1050 Fragments arranged by size: 1050 772 284 StuI AGG'CCT Cuts at: 0 821 2106 Size: 821 1285 StyI C'CwwG_G Cuts at: 0 471 2106 Size: 471 1635 TatI w'GTAC_w Cuts at: 0 1367 2106 Size: 1367 739 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 405 437 1928 1960 2106 Size: 405 32 1491 32 146 Fragments arranged by size: 1491 405 146 32 32 XhoI C'TCGA_G Cuts at: 0 312 2106 Size: 312 1794 XmnI GAAnn'nnTTC Cuts at: 0 1067 2106 Size: 1067 1039 ZraI GAC'GTC Cuts at: 0 1758 2106 Size: 1758 348 Enzymes that do cut: AatII AccI AcuI AfeI AflII AgeI AleI AlfI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI BglII Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI DraI DraIII EaeI EagI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII HpaI KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV PfoI PmlI PpiI PpuMI PspOMI PspXI PstI PvuII SapI SfcI SfoI SgrAI SmlI SspI StuI StyI TatI TstI XhoI XmnI ZraI Enzymes that do not cut: AarI Acc65I AclI AflIII AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI BlpI BmgBI BmrI BmtI BsaBI BsaXI BsiHKAI BsiWI BsmBI BspHI BsrDI BssHII BssSI BstBI BstEII BtgZI BtsI ClaI CspCI DrdI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII KpnI MfeI MluI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PshAI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI SwaI TaqII Tth111I XbaI XcmI XmaI