Restriction map for
pFN21AE1895
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AocI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AvrII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DdeI DraI
DraIII DrdI Eam1105I EarI Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HindIII
HpaI KpnI Ksp632I MamI MfeI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I XbaI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 1145 GGCGCC G[GA]^CG[CT]C
AfaI 1125 GTAC GT^AC
AlwNI 144 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1041 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1145 GGCGCC G^G[CT][GA]CC
BbeI 1145 GGCGCC GGCGC^C
BbsI 1219 GAAGAC GAAGAC(2/6)
BglI 494 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 809 AGATCT A^GATCT
BpuAI 1219 GAAGAC GAAGAC(2/6)
BsaHI 1145 GGCGCC G[GA]^CG[CT]C
BseRI 43 CTCCTC GAGGAG(10/8)
BssSI 540 CACGAG CACGAG(-5/-1)
BstAPI 143 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 719 GGATG GGATG(2/0)
BstYI 809 AGATCT [GA]^GATC[CT]
EcoT14I 761 CCTTGG C^C[AT][AT]GG
FokI 719 GGATG GGATG(9/13)
HgaI 1018 GACGC GACGC(5/10)
Hin1I 1145 GGCGCC G[GA]^CG[CT]C
HinfI 955 GAGTC G^A[ACGT]TC
KasI 1145 GGCGCC G^GCGCC
MaeII 1010 ACGT A^CGT
MflI 809 AGATCT [GA]^GATC[CT]
MslI 514 CATGGCGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 964 GCCGGC GCC^GGC
NarI 1145 GGCGCC GG^CGCC
NgoMIV 964 GCCGGC G^CCGGC
NotI 9 GCGGCCGC GC^GGCCGC
PflMI 822 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 955 GAGTC GAGTC(4/5)
PpuMI 487 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 837 CAGCTG CAG^CTG
RsaI 1125 GTAC GT^AC
SacI 917 GAGCTC GAGCT^C
SfoI 1145 GGCGCC GGC^GCC
SmaI 65 CCCGGG CCC^GGG
SmlI 1135 CTCAAG C^T[CT][GA]AG
StyI 761 CCTTGG C^C[AT][AT]GG
TaqI 1082 TCGA T^CGA
Tsp509I 256 AATT ^AATT
TthHB8I 1082 TCGA T^CGA
Van91I 822 CCAGTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 809 AGATCT [GA]^GATC[CT]
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
XmnI 1041 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Aor51HI 566 AGCGCT AGC^GCT
1139 AGCGCT
AspI 888 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1018 GACGCGGTC
AvaI 65 CCCGGG C^[CT]CG[GA]G
856 CCCGAG
AviII 1052 TGCGCA TGC^GCA
1226 TGCGCA
BfaI 424 CTAG C^TAG
784 CTAG
BsaI 859 GAGACC GGTCTC(1/5)
1115 GAGACC
BsgI 439 CTGCAC GTGCAG(16/14)
813 CTGCAC
BsiEI 10 CGGCCG CG[GA][CT]^CG
1104 CGGCCG
BsmAI 859 GAGAC GTCTC(1/5)
1115 GAGAC
BsoBI 65 CCCGGG C^[CT]CG[GA]G
856 CCCGAG
BsrI 822 CCAGT ACTGG(1/-1)
903 CCAGT
EagI 10 CGGCCG C^GGCCG
1104 CGGCCG
EclXI 10 CGGCCG C^GGCCG
1104 CGGCCG
Eco47III 566 AGCGCT AGC^GCT
1139 AGCGCT
Eco52I 10 CGGCCG C^GGCCG
1104 CGGCCG
FspI 1052 TGCGCA TGC^GCA
1226 TGCGCA
KspI 13 CCGCGG CCGC^GG
1160 CCGCGG
MaeI 424 CTAG C^TAG
784 CTAG
PflFI 888 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1018 GACGCGGTC
SacII 13 CCGCGG CCGC^GG
1160 CCGCGG
SfaNI 981 GATGC GCATC(5/9)
1131 GATGC
StuI 278 AGGCCT AGG^CCT
293 AGGCCT
Tth111I 888 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1018 GACGCGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AluI ApaI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BspMI BsrFI BssKI
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DpnII DraII DsaI EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI Sau3AI Sau96I ScrFI SfcI
TseI Tsp45I TspRI XcmI