################################## # NO CUT ENZYMES ( 134 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BamHI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaI BsaWI BseAI BsgI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MfeI MflI MluI MroI MseI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 393 GTATAC GT^[AC][GT]AC AflIII 287 ACATGT A^C[GA][CT]GT AlwNI 398 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG AvaII 846 GGACC G^G[AT]CC BalI 448 TGGCCA TGG^CCA BbeI 70 GGCGCC GGCGC^C BbrPI 559 CACGTG CAC^GTG BglI 110 GCCTGCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 195 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 781 GAGGAG GAGGAG(10/8) BsiYI 688 CCCATCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 688 CCCATCTGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 44 GTCTC GTCTC(1/5) BsmBI 43 CGTCTC CGTCTC(1/5) BspLU11I 287 ACATGT A^CATGT BsrFI 106 GCCGGC [GA]^CCGG[CT] BssHII 102 GCGCGC G^CGCGC Bst1107I 393 GTATAC GTA^TAC BstEII 597 GGTGACC G^GT[ACGT]ACC BstPI 597 GGTGACC G^GT[ACGT]ACC BstZ17I 393 GTATAC GTA^TAC Cfr10I 106 GCCGGC [GA]^CCGG[CT] DpnII 736 GATC ^GATC DsaI 812 CCACGG C^C[GA][CT]GG EagI 178 CGGCCG C^GGCCG EarI 801 GAAGAG CTCTTC(1/4) EclXI 178 CGGCCG C^GGCCG Eco52I 178 CGGCCG C^GGCCG EcoO65I 597 GGTGACC G^GT[ACGT]ACC EcoT14I 359 CCTTGG C^C[AT][AT]GG KasI 70 GGCGCC G^GCGCC Ksp632I 801 GAAGAG CTCTTC(1/4) MamI 195 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 736 GATC ^GATC MluNI 448 TGGCCA TGG^CCA MscI 448 TGGCCA TGG^CCA NaeI 106 GCCGGC GCC^GGC NarI 70 GGCGCC GG^CGCC NdeII 736 GATC ^GATC NgoMIV 106 GCCGGC G^CCGGC NspI 287 ACATGT [GA]CATG^[CT] PleI 270 GAGTC GAGTC(4/5) PmaCI 559 CACGTG CAC^GTG PmlI 559 CACGTG CAC^GTG PshAI 330 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 112 CTGCAG CTGCA^G PvuI 735 CGATCG CGAT^CG PvuII 539 CAGCTG CAG^CTG Sau3AI 736 GATC ^GATC SbfI 111 CCTGCAGG CCTGCA^GG SfcI 112 CTGCAG C^T[GA][CT]AG SfiI 109 GGCCTGCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 70 GGCGCC GGC^GCC Sse8387I 111 CCTGCAGG CCTGCA^GG StyI 359 CCTTGG C^C[AT][AT]GG TaqI 738 TCGA T^CGA TthHB8I 738 TCGA T^CGA XcmI 56 CCACCTTCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 70 GGCGCC G[GA]^CG[CT]C 697 GGCGTC AspHI 79 GTGCTC G[AT]GC[AT]^C 291 GTGCTC BanI 70 GGCGCC G^G[CT][GA]CC 807 GGTGCC BbsI 261 GTCTTC GAAGAC(2/6) 502 GTCTTC BpuAI 261 GTCTTC GAAGAC(2/6) 502 GTCTTC BsaAI 139 TACGTG [CT]AC^GT[GA] 559 CACGTG BsaHI 70 GGCGCC G[GA]^CG[CT]C 697 GGCGTC BsiEI 178 CGGCCG CG[GA][CT]^CG 735 CGATCG BsiHKAI 79 GTGCTC G[AT]GC[AT]^C 291 GTGCTC BstF5I 201 CATCC GGATG(2/0) 628 CATCC FauI 4 GCGGG CCCGC(4/6) 18 GCGGG FokI 201 CATCC GGATG(9/13) 628 CATCC HaeII 70 GGCGCC [GA]GCGC^[CT] 132 GGCGCT HgaI 42 GCGTC GACGC(5/10) 698 GCGTC Hin1I 70 GGCGCC G[GA]^CG[CT]C 697 GGCGTC MspA1I 91 CCGCTG C[AC]G^C[GT]G 539 CAGCTG NlaIII 288 CATG CATG^ 531 CATG SfaNI 675 GATGC GCATC(5/9) 627 GCATC Tsp509I 550 AATT ^AATT 765 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AccII AciI AfaI AluI BanII BbvI BcnI BmyI BpmI BsaJI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DdeI EaeI EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboII MnlI MspI MvaI MvnI MwoI NciI NlaIV RsaI Sau96I ScrFI TfiI TseI Tsp45I TspRI