(Circular) (Six-base) MAPSORT of: pFN21AE1776.seq Check: 7145 from: 1 to: 5557 pFN21AE1776 5557 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3367 278 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2399 2900 1937 Size: 462 501 4594 Fragments arranged by size: 4594 501 462 Acc65I G'GTAC_C Cuts at: 2878 2878 Size: 5557 AclI AA'CG_TT Cuts at: 1563 3684 4057 1563 Size: 2121 373 3063 Fragments arranged by size: 3063 2121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2483 3620 5004 5272 1632 Size: 851 1137 1384 268 1917 Fragments arranged by size: 1917 1384 1137 851 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5360 AflIII A'CryG_T Cuts at: 1203 2292 4456 5433 1203 Size: 1089 2164 977 1327 Fragments arranged by size: 2164 1327 1089 977 AgeI A'CCGG_T Cuts at: 4388 4388 Size: 5557 AhdI GACnn_n'nnGTC Cuts at: 4286 4286 Size: 5557 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5557 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5557 AlwNI CAG_nnn'CTG Cuts at: 2408 3300 4872 2408 Size: 892 1572 3093 Fragments arranged by size: 3093 1572 892 ApaLI G'TGCA_C Cuts at: 3614 4770 3614 Size: 1156 4401 ApoI r'AATT_y Cuts at: 1493 2866 3092 3244 5353 1493 Size: 1373 226 152 2109 1697 Fragments arranged by size: 2109 1697 1373 226 152 AseI AT'TA_AT Cuts at: 160 1032 4111 160 Size: 872 3079 1606 Fragments arranged by size: 3079 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 AvaI C'yCGr_G Cuts at: 1870 1943 2882 1870 Size: 73 939 4545 Fragments arranged by size: 4545 939 73 BamHI G'GATC_C Cuts at: 2887 3254 2887 Size: 367 5190 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2812 2878 4333 618 Size: 325 217 627 288 737 66 1455 1842 Fragments arranged by size: 1842 1455 737 627 325 288 217 66 BanII G_rGCy'C Cuts at: 1391 2029 2039 2157 2876 1391 Size: 638 10 118 719 4072 Fragments arranged by size: 4072 719 638 118 10 BbeI G_GCGC'C Cuts at: 2079 2079 Size: 5557 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2259 2500 928 Size: 971 360 241 3985 Fragments arranged by size: 3985 971 360 241 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3748 3782 1903 Size: 34 1811 34 3678 Fragments arranged by size: 3678 1811 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3449 4659 3449 Size: 1210 4347 BclI T'GATC_A Cuts at: 1548 1548 Size: 5557 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2121 4168 136 Size: 107 122 71 631 605 449 2047 1525 Fragments arranged by size: 2047 1525 631 605 449 122 107 71 BglII A'GATC_T Cuts at: 5552 5552 Size: 5557 BlpI GC'TnA_GC Cuts at: 2974 2974 Size: 5557 Bme1580I G_kGCm'C Cuts at: 1790 2817 3618 4774 1790 Size: 1027 801 1156 2573 Fragments arranged by size: 2573 1156 1027 801 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5557 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3288 4246 442 Size: 416 53 83 399 24 1871 958 1753 Fragments arranged by size: 1871 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5557 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2414 2680 2816 4217 1239 Size: 1175 266 136 1401 2579 Fragments arranged by size: 2579 1401 1175 266 136 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3682 4547 4845 3682 Size: 865 298 4394 Fragments arranged by size: 4394 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4220 882 Size: 617 2721 2219 Fragments arranged by size: 2721 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2146 2566 3348 493 Size: 711 942 420 782 2702 Fragments arranged by size: 2702 942 782 711 420 BsaBI GATnn'nnATC Cuts at: 2204 5551 2204 Size: 3347 2210 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 2703 3364 3746 Size: 53 83 186 936 543 627 661 382 Cuts at: 3746 4381 275 Size: 635 1451 Fragments arranged by size: 1451 936 661 635 627 543 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3990 4388 4662 4809 5220 3990 Size: 398 274 147 411 4327 Fragments arranged by size: 4327 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2282 2312 1178 Size: 30 165 30 879 30 4423 Fragments arranged by size: 4423 879 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2801 1186 Size: 1615 3942 BseYI C'CCAG_C Cuts at: 2071 4760 5256 2071 Size: 2689 496 2372 Fragments arranged by size: 2689 2372 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4984 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2186 2743 3007 3768 4796 1540 Size: 275 186 185 557 264 761 1028 2301 Fragments arranged by size: 2301 1028 761 557 275 264 186 185 BsiHKAI G_wGCw'C Cuts at: 2088 2300 3618 3703 4774 2088 Size: 212 1318 85 1071 2871 Fragments arranged by size: 2871 1318 1071 212 85 BsmI GAATG_Cn' Cuts at: 3068 3161 3068 Size: 93 5464 BsmBI CGTCTCn'nnnn_ Cuts at: 2054 2054 Size: 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2029 2039 2088 2157 2300 2817 2876 Size: 399 239 10 49 69 143 517 59 Cuts at: 2876 3618 3703 4774 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 517 399 239 143 85 69 59 49 10 BspEI T'CCGG_A Cuts at: 5220 5220 Size: 5557 BspHI T'CATG_A Cuts at: 3446 5176 3446 Size: 1730 3827 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2912 844 Size: 2068 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3444 1394 Size: 429 1621 3507 Fragments arranged by size: 3507 1621 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4052 4226 65 Size: 1183 2804 174 1396 Fragments arranged by size: 2804 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 4201 4388 1700 Size: 110 301 2090 187 2869 Fragments arranged by size: 2869 2090 301 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5557 BssHII G'CGCG_C Cuts at: 1925 2107 1925 Size: 182 5375 BssSI C'ACGA_G Cuts at: 3617 4629 3617 Size: 1012 4545 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5557 BstBI TT'CG_AA Cuts at: 4361 4361 Size: 5557 BstEII G'GTnAC_C Cuts at: 1184 2602 1184 Size: 1418 4139 BstYI r'GATC_y Cuts at: 1972 2887 3254 3639 3656 5108 5194 5206 5552 Size: 915 367 385 17 1452 86 12 346 Cuts at: 5552 1972 Size: 1977 Fragments arranged by size: 1977 1452 915 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2400 2400 Size: 5557 BtgI C'CryG_G Cuts at: 513 1065 2004 2817 513 Size: 552 939 813 3253 Fragments arranged by size: 3253 939 813 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4818 Fragments arranged by size: 4818 683 56 BtsI GCAGTG_nn' Cuts at: 3073 3867 3887 3073 Size: 794 20 4743 Fragments arranged by size: 4743 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5557 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5522 DraI TTT'AAA Cuts at: 2861 2861 Size: 5557 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4564 809 Size: 507 3248 1802 Fragments arranged by size: 3248 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2183 2453 Size: 54 1425 50 275 18 318 35 270 Cuts at: 2453 2532 3004 3893 5522 8 Size: 79 472 889 1629 43 Fragments arranged by size: 1629 1425 889 472 318 275 270 79 54 50 43 35 18 EagI C'GGCC_G Cuts at: 1537 1812 2183 3004 1537 Size: 275 371 821 4090 Fragments arranged by size: 4090 821 371 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2801 3487 1353 Size: 1448 686 3423 Fragments arranged by size: 3423 1448 686 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2019 4144 4518 4664 387 Size: 1064 138 430 2125 374 146 1280 Fragments arranged by size: 2125 1280 1064 430 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2414 2483 2680 2816 3620 4217 5004 Size: 393 782 69 197 136 804 597 787 Cuts at: 5004 5272 1239 Size: 268 1524 Fragments arranged by size: 1524 804 787 782 597 393 268 197 136 69 EcoRI G'AATT_C Cuts at: 2866 3244 2866 Size: 378 5179 FspI TGC'GCA Cuts at: 4063 4063 Size: 5557 HaeII r_GCGC'y Cuts at: 1678 2079 2141 4704 1678 Size: 401 62 2563 2531 Fragments arranged by size: 2563 2531 401 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4198 4230 4272 4304 1373 Size: 32 135 32 2626 32 42 32 2626 Fragments arranged by size: 2626 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2901 3147 669 Size: 988 281 963 246 3079 Fragments arranged by size: 3079 988 963 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5557 HpaI GTT'AAC Cuts at: 3147 3147 Size: 5557 KasI G'GCGC_C Cuts at: 2075 2075 Size: 5557 KpnI G_GTAC'C Cuts at: 2882 2882 Size: 5557 MfeI C'AATT_G Cuts at: 3156 3156 Size: 5557 MluI A'CGCG_T Cuts at: 5433 5433 Size: 5557 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4671 4855 1305 Size: 3366 184 2007 Fragments arranged by size: 3366 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2455 10 Size: 54 1425 343 623 3112 Fragments arranged by size: 3112 1425 623 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3515 3874 4033 518 Size: 648 2349 359 159 2042 Fragments arranged by size: 2349 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2546 2973 3650 4798 5043 1606 Size: 492 448 427 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 492 448 427 245 NaeI GCC'GGC Cuts at: 2113 2113 Size: 5557 NarI GG'CG_CC Cuts at: 2076 2076 Size: 5557 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4066 Fragments arranged by size: 4066 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5557 NgoMIV G'CCGG_C Cuts at: 2111 2111 Size: 5557 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5557 NotI GC'GGCC_GC Cuts at: 3004 3004 Size: 5557 NspI r_CATG'y Cuts at: 1132 1229 2296 2915 4460 1132 Size: 97 1067 619 1545 2229 Fragments arranged by size: 2229 1545 1067 619 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5557 PciI A'CATG_T Cuts at: 2292 4456 2292 Size: 2164 3393 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5557 PmeI GTTT'AAAC Cuts at: 2861 2861 Size: 5557 PmlI CAC'GTG Cuts at: 2566 2566 Size: 5557 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3626 3658 5139 5171 3626 Size: 32 1481 32 4012 Fragments arranged by size: 4012 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2339 1613 Size: 726 4831 PsiI TTA'TAA Cuts at: 3127 3127 Size: 5557 PstI C_TGCA'G Cuts at: 830 2121 2909 830 Size: 1291 788 3478 Fragments arranged by size: 3478 1291 788 PvuI CG_AT'CG Cuts at: 2001 2743 2001 Size: 742 4815 PvuII CAG'CTG Cuts at: 2546 2546 Size: 5557 SalI G'TCGA_C Cuts at: 1936 2899 1936 Size: 963 4594 SbfI CC_TGCA'GG Cuts at: 2121 2909 2121 Size: 788 4769 ScaI AGT'ACT Cuts at: 3805 5517 3805 Size: 1712 3845 SexAI A'CCwGG_T Cuts at: 2853 2853 Size: 5557 SfcI C'TryA_G Cuts at: 826 1045 2117 2905 4040 4721 4912 826 Size: 219 1072 788 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 788 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2121 2121 Size: 5557 SfoI GGC'GCC Cuts at: 2077 2077 Size: 5557 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5557 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5557 SmaI CCC'GGG Cuts at: 1872 2884 1872 Size: 1012 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3661 4562 4824 820 Size: 197 926 1718 901 262 1553 Fragments arranged by size: 1718 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3348 493 Size: 2855 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5557 SphI G_CATG'C Cuts at: 1132 2915 1132 Size: 1783 3774 SspI AAT'ATT Cuts at: 5 52 3481 5 Size: 47 3429 2081 Fragments arranged by size: 3429 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5557 StyI C'CwwG_G Cuts at: 513 1065 2004 2364 2996 513 Size: 552 939 360 632 3074 Fragments arranged by size: 3074 939 632 552 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3584 3601 3754 3939 4384 325 Size: 1255 2004 17 153 185 445 1498 Fragments arranged by size: 2004 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3803 5515 96 Size: 274 80 33 51 1443 1826 1712 138 Fragments arranged by size: 1826 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2661 2693 5517 5549 2661 Size: 32 2824 32 2669 Fragments arranged by size: 2824 2669 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5557 XbaI T'CTAG_A Cuts at: 2893 2893 Size: 5557 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2068 Size: 5557 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5557 XmaI C'CCGG_G Cuts at: 1870 2882 1870 Size: 1012 4545 XmnI GAAnn'nnTTC Cuts at: 3686 3686 Size: 5557 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3365 276 Size: 53 83 186 936 1831 2468 Fragments arranged by size: 2468 1831 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SrfI SwaI