################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## Acc65I AccIII AclI AflII AhdI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstXI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfoI SfuI SmaI SmlI SnaBI SpeI SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 1396 GACGTC GACGT^C AccI 1685 GTCTAC GT^[AC][GT]AC AcsI 1033 GAATTC [GA]^AATT[CT] AcyI 1396 GACGTC G[GA]^CG[CT]C AflIII 350 ACACGT A^C[GA][CT]GT AgeI 1208 ACCGGT A^CCGGT Alw44I 465 GTGCAC G^TGCAC AocI 987 CCTAAGG CC^T[ACGT]AGG Aor51HI 774 AGCGCT AGC^GCT ApaLI 465 GTGCAC G^TGCAC ApoI 1033 GAATTC [GA]^AATT[CT] BsaHI 1396 GACGTC G[GA]^CG[CT]C BsaWI 1208 ACCGGT [AT]^CCGG[AT] BsmI 1569 GAATGC GAATGC(1/-1) BsrDI 1620 CATTGC GCAATG(2/0) BstEII 1181 GGTCACC G^GT[ACGT]ACC BstPI 1181 GGTCACC G^GT[ACGT]ACC BstYI 1849 AGATCC [GA]^GATC[CT] Bsu36I 987 CCTAAGG CC^T[ACGT]AGG DraIII 565 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 774 AGCGCT AGC^GCT Eco81I 987 CCTAAGG CC^T[ACGT]AGG EcoNI 1724 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1181 GGTCACC G^GT[ACGT]ACC EcoRI 1033 GAATTC G^AATTC Hin1I 1396 GACGTC G[GA]^CG[CT]C MflI 1849 AGATCC [GA]^GATC[CT] MslI 1221 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 136 GCCGGC GCC^GGC NcoI 680 CCATGG C^CATGG NgoMIV 136 GCCGGC G^CCGGC NotI 975 GCGGCCGC GC^GGCCGC PflMI 60 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1208 ACCGGT A^CCGGT PpuMI 210 GGGTCCC [GA]G^G[AT]CC[CT] SacI 36 GAGCTC GAGCT^C SapI 1816 GAAGAGC GCTCTTC(1/4) SfiI 627 GGCCGTGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 227 CGCCGGTG C[GA]^CCGG[CT]G SphI 746 GCATGC GCATG^C TfiI 580 GAATC G^A[AT]TC Tsp509I 1034 AATT ^AATT Van91I 60 CCACCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1849 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 1850 GATCC GGATC(4/5) 1839 GGATC AvaI 33 CCCGAG C^[CT]CG[GA]G 51 CCCGAG BalI 848 TGGCCA TGG^CCA 908 TGGCCA BanI 1295 GGCACC G^G[CT][GA]CC 1659 GGCACC BbrPI 85 CACGTG CAC^GTG 351 CACGTG BcgI 126 CGACTTCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1460 GCATGAGGGTCG BcnI 558 CCGGG CC^[GC]GG 1752 CCCGG BsaI 331 GAGACC GGTCTC(1/5) 1349 GAGACC BsmBI 388 GAGACG CGTCTC(1/5) 1394 GAGACG BsoBI 33 CCCGAG C^[CT]CG[GA]G 51 CCCGAG DsaI 680 CCATGG C^C[GA][CT]GG 1664 CCGTGG EagI 165 CGGCCG C^GGCCG 976 CGGCCG EclXI 165 CGGCCG C^GGCCG 976 CGGCCG Eco52I 165 CGGCCG C^GGCCG 976 CGGCCG HgaI 390 GACGC GACGC(5/10) 721 GACGC MluNI 848 TGGCCA TGG^CCA 908 TGGCCA MscI 848 TGGCCA TGG^CCA 908 TGGCCA NciI 558 CCGGG CC^[GC]GG 1752 CCCGG PleI 1285 GACTC GAGTC(4/5) 1683 GAGTC PmaCI 85 CACGTG CAC^GTG 351 CACGTG PmlI 85 CACGTG CAC^GTG 351 CACGTG SbfI 867 CCTGCAGG CCTGCA^GG 1299 CCTGCAGG SexAI 878 ACCTGGT A^CC[AT]GGT 1328 ACCAGGT Sse8387I 867 CCTGCAGG CCTGCA^GG 1299 CCTGCAGG StuI 821 AGGCCT AGG^CCT 991 AGGCCT XcmI 296 CCAACTGTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1223 CCAAGGTGCTGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI ApaI AspHI AvaII BanII BbsI BbvCI BbvI BglI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrFI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI Tsp45I TspRI