(Linear) (Six-base) MAPSORT of: KIEE4173.seq Check: 4534 from: 1 to: 1902 KIEE4173.orf With 182 enzymes: SgfI * March 22, 2012 17:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 184 1307 1902 Size: 184 1123 595 Fragments arranged by size: 1123 595 184 AatII G_ACGT'C Cuts at: 0 1400 1902 Size: 1400 502 AccI GT'mk_AC Cuts at: 0 1686 1902 Size: 1686 216 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 1902 Size: 226 1676 AfeI AGC'GCT Cuts at: 0 776 1902 Size: 776 1126 AflIII A'CryG_T Cuts at: 0 350 1902 Size: 350 1552 AgeI A'CCGG_T Cuts at: 0 1208 1902 Size: 1208 694 AleI CACnn'nnGTG Cuts at: 0 1225 1902 Size: 1225 677 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1243 1284 1586 1902 Size: 1243 41 302 316 Fragments arranged by size: 1243 316 302 41 AlwNI CAG_nnn'CTG Cuts at: 0 252 1090 1341 1902 Size: 252 838 251 561 Fragments arranged by size: 838 561 252 251 ApaI G_GGCC'C Cuts at: 0 23 34 1148 1902 Size: 23 11 1114 754 Fragments arranged by size: 1114 754 23 11 ApaLI G'TGCA_C Cuts at: 0 465 1902 Size: 465 1437 ApoI r'AATT_y Cuts at: 0 1033 1902 Size: 1033 869 AvaI C'yCGr_G Cuts at: 0 33 51 1902 Size: 33 18 1851 Fragments arranged by size: 1851 33 18 BanI G'GyrC_C Cuts at: 0 1295 1659 1902 Size: 1295 364 243 Fragments arranged by size: 1295 364 243 BanII G_rGCy'C Cuts at: 0 23 34 40 224 485 1148 1649 1800 Size: 23 11 6 184 261 663 501 151 Cuts at: 1800 1902 Size: 102 Fragments arranged by size: 663 501 261 184 151 102 23 11 6 BbsI GAAGACnn'nnnn_ Cuts at: 0 100 1464 1774 1902 Size: 100 1364 310 128 Fragments arranged by size: 1364 310 128 100 BbvCI CC'TCA_GC Cuts at: 0 901 1558 1725 1902 Size: 901 657 167 177 Fragments arranged by size: 901 657 177 167 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 115 149 1449 1483 1902 Size: 115 34 1300 34 419 Fragments arranged by size: 1300 419 115 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 149 634 916 1902 Size: 149 485 282 986 Fragments arranged by size: 986 485 282 149 Bme1580I G_kGCm'C Cuts at: 0 23 34 76 458 469 564 1148 1902 Size: 23 11 42 382 11 95 584 754 Fragments arranged by size: 754 584 382 95 42 23 11 11 BmgBI CAC'GTC Cuts at: 0 327 453 1075 1425 1902 Size: 327 126 622 350 477 Fragments arranged by size: 622 477 350 327 126 BmrI ACTGGGnnnn_n' Cuts at: 0 1156 1902 Size: 1156 746 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 855 1464 1902 Size: 855 609 438 Fragments arranged by size: 855 609 438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 328 631 1069 1537 1663 1902 Size: 46 282 303 438 468 126 239 Fragments arranged by size: 468 438 303 282 239 126 46 Bpu10I CC'TnA_GC Cuts at: 0 264 901 1122 1558 1725 1902 Size: 264 637 221 436 167 177 Fragments arranged by size: 637 436 264 221 177 167 BsaI GGTCTCn'nnnn_ Cuts at: 0 325 1343 1902 Size: 325 1018 559 Fragments arranged by size: 1018 559 325 BsaAI yAC'GTr Cuts at: 0 87 338 353 591 1902 Size: 87 251 15 238 1311 Fragments arranged by size: 1311 251 238 87 15 BsaHI Gr'CG_yC Cuts at: 0 1397 1902 Size: 1397 505 BsaWI w'CCGG_w Cuts at: 0 1208 1902 Size: 1208 694 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 322 352 1141 1171 1600 1630 1636 1666 Size: 322 30 789 30 429 30 6 30 Cuts at: 1666 1902 Size: 236 Fragments arranged by size: 789 429 322 236 30 30 30 30 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 703 727 730 882 1902 Size: 703 24 3 152 1020 Fragments arranged by size: 1020 703 152 24 3 BseYI C'CCAG_C Cuts at: 0 214 1065 1535 1793 1902 Size: 214 851 470 258 109 Fragments arranged by size: 851 470 258 214 109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 109 333 457 613 814 1189 1327 1387 Size: 109 224 124 156 201 375 138 60 Cuts at: 1387 1447 1902 Size: 60 455 Fragments arranged by size: 455 375 224 201 156 138 124 109 60 60 BsiEI CG_ry'CG Cuts at: 0 161 168 979 1902 Size: 161 7 811 923 Fragments arranged by size: 923 811 161 7 BsiHKAI G_wGCw'C Cuts at: 0 40 402 469 1268 1445 1902 Size: 40 362 67 799 177 457 Fragments arranged by size: 799 457 362 177 67 40 BsmI GAATG_Cn' Cuts at: 0 1575 1902 Size: 1575 327 BsmBI CGTCTCn'nnnn_ Cuts at: 0 382 1388 1902 Size: 382 1006 514 Fragments arranged by size: 1006 514 382 Bsp1286I G_dGCh'C Cuts at: 0 23 34 40 76 224 402 458 469 Size: 23 11 6 36 148 178 56 11 Cuts at: 469 485 564 1148 1268 1445 1649 1800 1902 Size: 16 79 584 120 177 204 151 102 Fragments arranged by size: 584 204 178 177 151 148 120 102 79 56 36 23 16 11 11 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 184 861 1307 1902 Size: 184 677 446 595 Fragments arranged by size: 677 595 446 184 BsrBI CCG'CTC Cuts at: 0 606 1616 1638 1902 Size: 606 1010 22 264 Fragments arranged by size: 1010 606 264 22 BsrDI GCAATG_nn' Cuts at: 0 1619 1902 Size: 1619 283 BsrFI r'CCGG_y Cuts at: 0 136 228 236 1208 1292 1902 Size: 136 92 8 972 84 610 Fragments arranged by size: 972 610 136 92 84 8 BssHII G'CGCG_C Cuts at: 0 371 373 412 1039 1902 Size: 371 2 39 627 863 Fragments arranged by size: 863 627 371 39 2 BstAPI GCAn_nnn'nTGC Cuts at: 0 353 530 621 792 1090 1099 1902 Size: 353 177 91 171 298 9 803 Fragments arranged by size: 803 353 298 177 171 91 9 BstEII G'GTnAC_C Cuts at: 0 1181 1902 Size: 1181 721 BstYI r'GATC_y Cuts at: 0 1849 1902 Size: 1849 53 Bsu36I CC'TnA_GG Cuts at: 0 988 1902 Size: 988 914 BtgI C'CryG_G Cuts at: 0 680 1664 1902 Size: 680 984 238 Fragments arranged by size: 984 680 238 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 648 1886 1902 Size: 648 1238 16 Fragments arranged by size: 1238 648 16 BtsI GCAGTG_nn' Cuts at: 0 564 1054 1149 1266 1902 Size: 564 490 95 117 636 Fragments arranged by size: 636 564 490 117 95 DraIII CAC_nnn'GTG Cuts at: 0 570 1902 Size: 570 1332 EaeI y'GGCC_r Cuts at: 0 150 165 626 647 848 908 976 1902 Size: 150 15 461 21 201 60 68 926 Fragments arranged by size: 926 461 201 150 68 60 21 15 EagI C'GGCC_G Cuts at: 0 165 976 1902 Size: 165 811 926 Fragments arranged by size: 926 811 165 EarI CTCTTCn'nnn_ Cuts at: 0 742 803 1811 1902 Size: 742 61 1008 91 Fragments arranged by size: 1008 742 91 61 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1252 1396 1902 Size: 1252 144 506 Fragments arranged by size: 1252 506 144 EcoICRI GAG'CTC Cuts at: 0 38 1902 Size: 38 1864 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 226 328 631 1069 1537 1663 1902 Size: 46 180 102 303 438 468 126 239 Fragments arranged by size: 468 438 303 239 180 126 102 46 EcoNI CCTnn'n_nnAGG Cuts at: 0 1728 1902 Size: 1728 174 EcoO109I rG'GnC_Cy Cuts at: 0 20 30 211 636 1126 1144 1145 1902 Size: 20 10 181 425 490 18 1 757 Fragments arranged by size: 757 490 425 181 20 18 10 1 EcoRI G'AATT_C Cuts at: 0 1033 1902 Size: 1033 869 HaeII r_GCGC'y Cuts at: 0 102 707 778 1138 1902 Size: 102 605 71 360 764 Fragments arranged by size: 764 605 360 102 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 565 597 1660 1692 1756 1788 1902 Size: 565 32 1063 32 64 32 114 Fragments arranged by size: 1063 565 114 64 32 32 32 MscI TGG'CCA Cuts at: 0 850 910 1902 Size: 850 60 992 Fragments arranged by size: 992 850 60 MslI CAynn'nnrTG Cuts at: 0 1225 1902 Size: 1225 677 MspA1I CmG'CkG Cuts at: 0 66 78 183 218 530 1087 1557 1793 Size: 66 12 105 35 312 557 470 236 Cuts at: 1793 1902 Size: 109 Fragments arranged by size: 557 470 312 236 109 105 66 35 12 NaeI GCC'GGC Cuts at: 0 138 1902 Size: 138 1764 NcoI C'CATG_G Cuts at: 0 680 1902 Size: 680 1222 NgoMIV G'CCGG_C Cuts at: 0 136 1902 Size: 136 1766 NotI GC'GGCC_GC Cuts at: 0 976 1902 Size: 976 926 NspI r_CATG'y Cuts at: 0 408 546 750 1902 Size: 408 138 204 1152 Fragments arranged by size: 1152 408 204 138 PasI CC'CwG_GG Cuts at: 0 432 1902 Size: 432 1470 PflMI CCAn_nnn'nTGG Cuts at: 0 66 1902 Size: 66 1836 PfoI T'CCnGG_A Cuts at: 0 926 1475 1740 1751 1902 Size: 926 549 265 11 151 Fragments arranged by size: 926 549 265 151 11 PmlI CAC'GTG Cuts at: 0 87 353 1902 Size: 87 266 1549 Fragments arranged by size: 1549 266 87 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1795 1827 1902 Size: 1795 32 75 Fragments arranged by size: 1795 75 32 PpuMI rG'GwC_Cy Cuts at: 0 211 1902 Size: 211 1691 PspOMI G'GGCC_C Cuts at: 0 19 30 1144 1902 Size: 19 11 1114 758 Fragments arranged by size: 1114 758 19 11 PstI C_TGCA'G Cuts at: 0 248 644 677 788 872 962 1304 1902 Size: 248 396 33 111 84 90 342 598 Fragments arranged by size: 598 396 342 248 111 90 84 33 PvuII CAG'CTG Cuts at: 0 1087 1557 1793 1902 Size: 1087 470 236 109 Fragments arranged by size: 1087 470 236 109 SacI G_AGCT'C Cuts at: 0 40 1902 Size: 40 1862 SanDI GG'GwC_CC Cuts at: 0 211 1902 Size: 211 1691 SapI GCTCTTCn'nnn_ Cuts at: 0 1811 1902 Size: 1811 91 SbfI CC_TGCA'GG Cuts at: 0 872 1304 1902 Size: 872 432 598 Fragments arranged by size: 872 598 432 SexAI A'CCwGG_T Cuts at: 0 878 1328 1902 Size: 878 450 574 Fragments arranged by size: 878 574 450 SfcI C'TryA_G Cuts at: 0 244 640 673 784 868 958 1300 1687 Size: 244 396 33 111 84 90 342 387 Cuts at: 1687 1902 Size: 215 Fragments arranged by size: 396 387 342 244 215 111 90 84 33 SfiI GGCCn_nnn'nGGCC Cuts at: 0 634 1902 Size: 634 1268 SgrAI Cr'CCGG_yG Cuts at: 0 228 1902 Size: 228 1674 SphI G_CATG'C Cuts at: 0 750 1902 Size: 750 1152 StuI AGG'CCT Cuts at: 0 823 993 1902 Size: 823 170 909 Fragments arranged by size: 909 823 170 StyI C'CwwG_G Cuts at: 0 680 1130 1223 1625 1902 Size: 680 450 93 402 277 Fragments arranged by size: 680 450 402 277 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 175 1902 Size: 175 1727 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1141 1173 1902 Size: 1141 32 729 Fragments arranged by size: 1141 729 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 303 1230 1902 Size: 303 927 672 Fragments arranged by size: 927 672 303 ZraI GAC'GTC Cuts at: 0 1398 1902 Size: 1398 504 Enzymes that do cut: AarI AatII AccI AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BstAPI BstEII BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV NotI NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SanDI SapI SbfI SexAI SfcI SfiI SgrAI SphI StuI StyI TaqII TstI XcmI ZraI Enzymes that do not cut: Acc65I AclI AflII AhdI AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII BlpI BmtI BpuEI BsaBI BsiWI BspEI BspHI BsrGI BssSI BstBI BstXI BstZ17I ClaI CspCI DraI DrdI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI ScaI SfoI SgfI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TatI Tth111I XbaI XhoI XmaI XmnI