################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaBI BsaWI BseAI BseRI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstXI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I KspI MaeI MamI MfeI MluI MroI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AatII 418 GACGTC GACGT^C AcsI 595 GAATTC [GA]^AATT[CT] AflIII 911 ACACGT A^C[GA][CT]GT AhdI 172 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1102 GTGCAC G^TGCAC AlwI 951 GATCC GGATC(4/5) ApaLI 1102 GTGCAC G^TGCAC ApoI 595 GAATTC [GA]^AATT[CT] AspEI 172 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 535 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1221 TGGCCA TGG^CCA BbeI 186 GGCGCC GGCGC^C BbrPI 409 CACGTG CAC^GTG BglI 373 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 54 GCTGAGC GC^T[ACGT]AGC Bpu1102I 54 GCTGAGC GC^T[ACGT]AGC BsaI 1278 GGTCTC GGTCTC(1/5) BsgI 1293 CTGCAC GTGCAG(16/14) BsiEI 753 CGACCG CG[GA][CT]^CG BsmBI 902 GAGACG CGTCTC(1/5) Bsp1407I 611 TGTACA T^GTACA BspMI 699 GCAGGT ACCTGC(4/8) BsrBI 289 CCGCTC CCGCTC(-3/-3) BsrGI 611 TGTACA T^GTACA BssSI 442 CACGAG CACGAG(-5/-1) BstEII 13 GGTGACC G^GT[ACGT]ACC BstPI 13 GGTGACC G^GT[ACGT]ACC BstYI 950 AGATCC [GA]^GATC[CT] CelII 54 GCTGAGC GC^T[ACGT]AGC DdeI 55 CTGAG C^T[ACGT]AG DraIII 1225 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 172 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 13 GGTGACC G^GT[ACGT]ACC EcoRI 595 GAATTC G^AATTC HgaI 557 GCGTC GACGC(5/10) HincII 1132 GTCAAC GT[CT]^[GA]AC HindII 1132 GTCAAC GT[CT]^[GA]AC KasI 186 GGCGCC G^GCGCC MflI 950 AGATCC [GA]^GATC[CT] MluNI 1221 TGGCCA TGG^CCA MscI 1221 TGGCCA TGG^CCA MslI 495 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 183 GCCGGC GCC^GGC NarI 186 GGCGCC GG^CGCC NgoMIV 183 GCCGGC G^CCGGC NspI 325 ACATGC [GA]CATG^[CT] PflFI 535 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 409 CACGTG CAC^GTG PmlI 409 CACGTG CAC^GTG PshAI 414 GACCGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfoI 186 GGCGCC GGC^GCC SgrAI 182 CGCCGGCG C[GA]^CCGG[CT]G SmaI 1201 CCCGGG CCC^GGG SspBI 611 TGTACA T^GTACA TaqI 170 TCGA T^CGA Tsp509I 596 AATT ^AATT Tth111I 535 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 170 TCGA T^CGA XhoII 950 AGATCC [GA]^GATC[CT] XmaCI 1201 CCCGGG C^CCGGG XmaI 1201 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AcyI 186 GGCGCC G[GA]^CG[CT]C 418 GACGTC BsaAI 409 CACGTG [CT]AC^GT[GA] 912 CACGTA BsaHI 186 GGCGCC G[GA]^CG[CT]C 418 GACGTC BsmAI 902 GAGAC GTCTC(1/5) 1279 GTCTC HaeII 186 GGCGCC [GA]GCGC^[CT] 1264 AGCGCC Hin1I 186 GGCGCC G[GA]^CG[CT]C 418 GACGTC HinfI 1012 GACTC G^A[ACGT]TC 1050 GAGTC MboII 297 GAAGA GAAGA(8/7) 885 GAAGA MspA1I 633 CAGCTG C[AC]G^C[GT]G 1138 CAGCTG PflMI 1137 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1269 CCAGTCCATGG PleI 1012 GACTC GAGTC(4/5) 1050 GAGTC PpuMI 1285 GGGTCCC [GA]G^G[AT]CC[CT] 1336 AGGACCT PvuII 633 CAGCTG CAG^CTG 1138 CAGCTG SacI 445 GAGCTC GAGCT^C 804 GAGCTC SfcI 1039 CTACAG C^T[GA][CT]AG 1057 CTACAG Van91I 1137 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1269 CCAGTCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MnlI MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI StyI TseI Tsp45I TspRI