(Circular) (Six-base) MAPSORT of: pFN21AE2616.seq Check: 5967 from: 1 to: 6055 pFN21AE2616 6055 bp With 182 enzymes: SgfI * April 1, 2016 16:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2695 2695 Size: 6055 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2427 3865 278 Size: 53 83 186 936 891 1438 2468 Fragments arranged by size: 2468 1438 936 891 186 83 53 AccI GT'mk_AC Cuts at: 1937 3398 1937 Size: 1461 4594 Acc65I G'GTAC_C Cuts at: 3376 3376 Size: 6055 AclI AA'CG_TT Cuts at: 1563 4182 4555 1563 Size: 2619 373 3063 Fragments arranged by size: 3063 2619 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2912 4118 5502 5770 1632 Size: 1280 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1280 1206 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5858 AflIII A'CryG_T Cuts at: 1203 2916 4954 5931 1203 Size: 1713 2038 977 1327 Fragments arranged by size: 2038 1713 1327 977 AgeI A'CCGG_T Cuts at: 4886 4886 Size: 6055 AhdI GACnn_n'nnGTC Cuts at: 2182 4784 2182 Size: 2602 3453 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6055 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6055 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2795 2827 2795 Size: 32 6023 AlwNI CAG_nnn'CTG Cuts at: 2375 2640 2710 2800 3010 3345 3798 5370 2375 Size: 265 70 90 210 335 453 1572 3060 Fragments arranged by size: 3060 1572 453 335 265 210 90 70 ApaI G_GGCC'C Cuts at: 3198 3206 3354 3198 Size: 8 148 5899 Fragments arranged by size: 5899 148 8 ApaLI G'TGCA_C Cuts at: 3107 4112 5268 3107 Size: 1005 1156 3894 Fragments arranged by size: 3894 1156 1005 ApoI r'AATT_y Cuts at: 1493 2600 3364 3590 3742 5851 1493 Size: 1107 764 226 152 2109 1697 Fragments arranged by size: 2109 1697 1107 764 226 152 AseI AT'TA_AT Cuts at: 160 1032 4609 160 Size: 872 3577 1606 Fragments arranged by size: 3577 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 AvaI C'yCGr_G Cuts at: 1870 1943 2453 3206 3286 3380 1870 Size: 73 510 753 80 94 4545 Fragments arranged by size: 4545 753 510 94 80 73 BamHI G'GATC_C Cuts at: 3385 3752 3385 Size: 367 5688 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2031 2191 2569 2879 2998 Size: 325 217 627 244 160 378 310 119 Cuts at: 2998 3376 4831 618 Size: 378 1455 1842 Fragments arranged by size: 1842 1455 627 378 378 325 310 244 217 160 119 BanII G_rGCy'C Cuts at: 1391 2454 2813 2858 3198 3206 3354 3374 1391 Size: 1063 359 45 340 8 148 20 4072 Fragments arranged by size: 4072 1063 359 340 148 45 20 8 BbeI G_GCGC'C Cuts at: 2195 2195 Size: 6055 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5084 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4246 4280 1903 Size: 34 50 34 2225 34 3678 Fragments arranged by size: 3678 2225 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3947 5157 3947 Size: 1210 4845 BclI T'GATC_A Cuts at: 1548 1548 Size: 6055 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2384 4666 136 Size: 107 122 71 631 605 712 2282 1525 Fragments arranged by size: 2282 1525 712 631 605 122 107 71 BglII A'GATC_T Cuts at: 6050 6050 Size: 6055 BlpI GC'TnA_GC Cuts at: 2060 3472 2060 Size: 1412 4643 Bme1580I G_kGCm'C Cuts at: 1790 2139 2574 3111 3198 3206 3354 4116 5272 Size: 349 435 537 87 8 148 762 1156 Cuts at: 5272 1790 Size: 2573 Fragments arranged by size: 2573 1156 762 537 435 349 148 87 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6055 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2041 3268 3786 Size: 416 53 83 399 24 624 1227 518 Cuts at: 3786 4744 442 Size: 958 1753 Fragments arranged by size: 1753 1227 958 624 518 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2469 2708 3002 4715 1239 Size: 1230 239 294 1713 2579 Fragments arranged by size: 2579 1713 1230 294 239 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4180 5045 5343 4180 Size: 865 298 4892 Fragments arranged by size: 4892 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3289 4718 882 Size: 617 1790 1429 2219 Fragments arranged by size: 2219 1790 1429 617 BsaAI yAC'GTr Cuts at: 493 1204 2416 2919 3846 493 Size: 711 1212 503 927 2702 Fragments arranged by size: 2702 1212 927 711 503 BsaBI GATnn'nnATC Cuts at: 6049 6049 Size: 6055 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2192 2424 3862 4244 Size: 53 83 186 936 659 232 1438 382 Cuts at: 4244 4879 275 Size: 635 1451 Fragments arranged by size: 1451 1438 936 659 635 382 232 186 83 53 BsaWI w'CCGG_w Cuts at: 4488 4886 5160 5307 5718 4488 Size: 398 274 147 411 4825 Fragments arranged by size: 4825 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2795 2825 1178 Size: 30 165 30 1392 30 4408 Fragments arranged by size: 4408 1392 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6055 BseYI C'CCAG_C Cuts at: 2162 2710 3329 5258 5754 2162 Size: 548 619 1929 496 2463 Fragments arranged by size: 2463 1929 619 548 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3283 1163 Size: 573 1547 3935 Fragments arranged by size: 3935 1547 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2761 3505 4266 5294 1540 Size: 275 186 760 744 761 1028 2301 Fragments arranged by size: 2301 1028 761 760 744 275 186 BsiHKAI G_wGCw'C Cuts at: 2124 2415 2454 2813 3111 4116 4201 5272 2124 Size: 291 39 359 298 1005 85 1071 2907 Fragments arranged by size: 2907 1071 1005 359 298 291 85 39 BsmI GAATG_Cn' Cuts at: 3566 3659 3566 Size: 93 5962 BsmBI CGTCTCn'nnnn_ Cuts at: 2901 2901 Size: 6055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 2139 2415 2454 2574 2813 2858 Size: 399 334 15 276 39 120 239 45 Cuts at: 2858 3111 3198 3206 3354 3374 4116 4201 5272 Size: 253 87 8 148 20 742 85 1071 Cuts at: 5272 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 334 276 253 239 148 120 87 85 45 39 20 15 8 BspEI T'CCGG_A Cuts at: 5718 5718 Size: 6055 BspHI T'CATG_A Cuts at: 3944 5674 3944 Size: 1730 4325 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2695 3410 844 Size: 1851 715 3489 Fragments arranged by size: 3489 1851 715 BsrBI CCG'CTC Cuts at: 1394 1823 2296 3942 1394 Size: 429 473 1646 3507 Fragments arranged by size: 3507 1646 473 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4550 4724 65 Size: 1183 3302 174 1396 Fragments arranged by size: 3302 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2188 2549 2658 2718 2882 3082 4699 Size: 110 378 361 109 60 164 200 1617 Cuts at: 4699 4886 1700 Size: 187 2869 Fragments arranged by size: 2869 1617 378 361 200 187 164 110 109 60 BsrGI T'GTAC_A Cuts at: 96 2616 96 Size: 2520 3535 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6055 BssSI C'ACGA_G Cuts at: 2447 4115 5127 2447 Size: 1668 1012 3375 Fragments arranged by size: 3375 1668 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6055 BstBI TT'CG_AA Cuts at: 4859 4859 Size: 6055 BstEII G'GTnAC_C Cuts at: 1184 2018 1184 Size: 834 5221 BstYI r'GATC_y Cuts at: 1972 2955 3385 3752 4137 4154 5606 5692 5704 Size: 983 430 367 385 17 1452 86 12 Cuts at: 5704 6050 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 983 430 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2040 2070 3198 3223 3279 513 Size: 552 975 30 1128 25 56 3289 Fragments arranged by size: 3289 1128 975 552 56 30 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5316 Fragments arranged by size: 5316 683 56 BtsI GCAGTG_nn' Cuts at: 2373 3240 3295 3571 4365 4385 2373 Size: 867 55 276 794 20 4043 Fragments arranged by size: 4043 867 794 276 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6055 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6020 DraI TTT'AAA Cuts at: 3359 3359 Size: 6055 DraIII CAC_nnn'GTG Cuts at: 3235 3235 Size: 6055 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5062 809 Size: 507 3746 1802 Fragments arranged by size: 3746 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2265 2752 Size: 54 1425 50 275 18 270 165 487 Cuts at: 2752 3226 3502 4391 6020 8 Size: 474 276 889 1629 43 Fragments arranged by size: 1629 1425 889 487 474 276 275 270 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3502 1537 Size: 275 1690 4090 Fragments arranged by size: 4090 1690 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3985 1353 Size: 2632 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2803 3294 4642 5016 5162 387 Size: 1064 138 1214 491 1348 374 146 1280 Fragments arranged by size: 1348 1280 1214 1064 491 374 146 138 EcoICRI GAG'CTC Cuts at: 2452 2811 2452 Size: 359 5696 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2469 2708 2912 3002 4118 4715 5502 Size: 393 837 239 204 90 1116 597 787 Cuts at: 5502 5770 1239 Size: 268 1524 Fragments arranged by size: 1524 1116 837 787 597 393 268 239 204 90 EcoO109I rG'GnC_Cy Cuts at: 2797 3194 3203 3291 3342 3350 2797 Size: 397 9 88 51 8 5502 Fragments arranged by size: 5502 397 88 51 9 8 EcoRI G'AATT_C Cuts at: 2600 3364 3742 2600 Size: 764 378 4913 Fragments arranged by size: 4913 764 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2860 2860 Size: 6055 FspI TGC'GCA Cuts at: 4561 4561 Size: 6055 HaeII r_GCGC'y Cuts at: 1678 2195 3273 5202 1678 Size: 517 1078 1929 2531 Fragments arranged by size: 2531 1929 1078 517 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2168 2200 3040 3072 4696 Size: 32 135 32 596 32 840 32 1624 Cuts at: 4696 4728 4770 4802 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1624 840 596 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3139 3399 3645 669 Size: 988 281 1201 260 246 3079 Fragments arranged by size: 3079 1201 988 281 260 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6055 HpaI GTT'AAC Cuts at: 3645 3645 Size: 6055 KasI G'GCGC_C Cuts at: 2191 2191 Size: 6055 KpnI G_GTAC'C Cuts at: 3380 3380 Size: 6055 MfeI C'AATT_G Cuts at: 3654 3654 Size: 6055 MluI A'CGCG_T Cuts at: 5931 5931 Size: 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 5169 5353 1305 Size: 927 2937 184 2007 Fragments arranged by size: 2937 2007 927 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3228 10 Size: 54 1425 343 1396 2837 Fragments arranged by size: 2837 1425 1396 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2504 4013 4372 4531 518 Size: 648 1338 1509 359 159 2042 Fragments arranged by size: 2042 1509 1338 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2640 3145 3471 4148 5296 5541 1606 Size: 1034 505 326 677 1148 245 2120 Fragments arranged by size: 2120 1148 1034 677 505 326 245 NaeI GCC'GGC Cuts at: 2190 2190 Size: 6055 NarI GG'CG_CC Cuts at: 2192 2192 Size: 6055 NcoI C'CATG_G Cuts at: 513 1065 2070 3223 3279 513 Size: 552 1005 1153 56 3289 Fragments arranged by size: 3289 1153 1005 552 56 NdeI CA'TA_TG Cuts at: 387 387 Size: 6055 NgoMIV G'CCGG_C Cuts at: 2188 2188 Size: 6055 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6055 NotI GC'GGCC_GC Cuts at: 3502 3502 Size: 6055 NspI r_CATG'y Cuts at: 1132 1229 2334 3413 4958 1132 Size: 97 1105 1079 1545 2229 Fragments arranged by size: 2229 1545 1105 1079 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6055 PciI A'CATG_T Cuts at: 4954 4954 Size: 6055 PflMI CCAn_nnn'nTGG Cuts at: 1108 3148 3280 1108 Size: 2040 132 3883 Fragments arranged by size: 3883 2040 132 PmeI GTTT'AAAC Cuts at: 3359 3359 Size: 6055 PmlI CAC'GTG Cuts at: 2416 2416 Size: 6055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2795 2827 4124 4156 5637 5669 2795 Size: 32 1297 32 1481 32 3181 Fragments arranged by size: 3181 1481 1297 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3291 3342 3291 Size: 51 6004 PshAI GACnn'nnGTC Cuts at: 1613 2423 1613 Size: 810 5245 PsiI TTA'TAA Cuts at: 3625 3625 Size: 6055 PspOMI G'GGCC_C Cuts at: 3194 3202 3350 3194 Size: 8 148 5899 Fragments arranged by size: 5899 148 8 PstI C_TGCA'G Cuts at: 830 3407 830 Size: 2577 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6055 PvuII CAG'CTG Cuts at: 2640 3145 2640 Size: 505 5550 SacI G_AGCT'C Cuts at: 2454 2813 2454 Size: 359 5696 SalI G'TCGA_C Cuts at: 1936 3397 1936 Size: 1461 4594 SanDI GG'GwC_CC Cuts at: 3291 3291 Size: 6055 SbfI CC_TGCA'GG Cuts at: 3407 3407 Size: 6055 ScaI AGT'ACT Cuts at: 4303 6015 4303 Size: 1712 4343 SfcI C'TryA_G Cuts at: 826 1045 3044 3062 3403 4538 5219 5410 826 Size: 219 1999 18 341 1135 681 191 1471 Fragments arranged by size: 1999 1471 1135 681 341 219 191 18 SfoI GGC'GCC Cuts at: 2193 2193 Size: 6055 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6055 SgrAI Cr'CCGG_yG Cuts at: 1700 2188 1700 Size: 488 5567 SmaI CCC'GGG Cuts at: 1872 3208 3382 1872 Size: 1336 174 4545 Fragments arranged by size: 4545 1336 174 SmlI C'TyrA_G Cuts at: 820 1017 1943 4159 5060 5322 820 Size: 197 926 2216 901 262 1553 Fragments arranged by size: 2216 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3846 493 Size: 3353 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6055 SphI G_CATG'C Cuts at: 1132 3413 1132 Size: 2281 3774 SspI AAT'ATT Cuts at: 5 52 3979 5 Size: 47 3927 2081 Fragments arranged by size: 3927 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6055 StyI C'CwwG_G Cuts at: 513 1065 2070 2875 3223 3279 3494 513 Size: 552 1005 805 348 56 215 3074 Fragments arranged by size: 3074 1005 805 552 348 215 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2156 2435 2775 3199 4082 4099 4252 Size: 1255 576 279 340 424 883 17 153 Cuts at: 4252 4437 4882 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 883 576 445 424 340 279 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2616 4301 6013 Size: 274 80 33 51 1443 639 1685 1712 Cuts at: 6013 96 Size: 138 Fragments arranged by size: 1712 1685 1443 639 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6015 6047 6015 Size: 32 6023 Tth111I GACn'n_nGTC Cuts at: 1316 2543 1316 Size: 1227 4828 XbaI T'CTAG_A Cuts at: 3391 3391 Size: 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6055 XmaI C'CCGG_G Cuts at: 1870 3206 3380 1870 Size: 1336 174 4545 Fragments arranged by size: 4545 1336 174 XmnI GAAnn'nnTTC Cuts at: 4184 4184 Size: 6055 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2425 3863 276 Size: 53 83 186 936 891 1438 2468 Fragments arranged by size: 2468 1438 936 891 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI XcmI