(Linear) (Six-base) MAPSORT of: KIEE2616.seq Check: 2524 from: 1 to: 1350 KIEE2616.orf With 182 enzymes: SgfI * April 1, 2016 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 690 1350 Size: 690 660 AatII G_ACGT'C Cuts at: 0 422 1350 Size: 422 928 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 907 1350 Size: 907 443 AflIII A'CryG_T Cuts at: 0 911 1350 Size: 911 439 AhdI GACnn_n'nnGTC Cuts at: 0 177 1350 Size: 177 1173 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 790 822 1350 Size: 790 32 528 Fragments arranged by size: 790 528 32 AlwNI CAG_nnn'CTG Cuts at: 0 370 635 705 795 1005 1340 1350 Size: 370 265 70 90 210 335 10 Fragments arranged by size: 370 335 265 210 90 70 10 ApaI G_GGCC'C Cuts at: 0 1193 1201 1349 1350 Size: 1193 8 148 1 Fragments arranged by size: 1193 148 8 1 ApaLI G'TGCA_C Cuts at: 0 1102 1350 Size: 1102 248 ApoI r'AATT_y Cuts at: 0 595 1350 Size: 595 755 AvaI C'yCGr_G Cuts at: 0 448 1201 1281 1350 Size: 448 753 80 69 Fragments arranged by size: 753 448 80 69 BanI G'GyrC_C Cuts at: 0 26 186 564 874 993 1350 Size: 26 160 378 310 119 357 Fragments arranged by size: 378 357 310 160 119 26 BanII G_rGCy'C Cuts at: 0 449 808 853 1193 1201 1349 1350 Size: 449 359 45 340 8 148 1 Fragments arranged by size: 449 359 340 148 45 8 1 BbeI G_GCGC'C Cuts at: 0 190 1350 Size: 190 1160 BglI GCCn_nnn'nGGC Cuts at: 0 379 1350 Size: 379 971 BlpI GC'TnA_GC Cuts at: 0 55 1350 Size: 55 1295 Bme1580I G_kGCm'C Cuts at: 0 134 569 1106 1193 1201 1349 1350 Size: 134 435 537 87 8 148 1 Fragments arranged by size: 537 435 148 134 87 8 1 BmrI ACTGGGnnnn_n' Cuts at: 0 36 1263 1350 Size: 36 1227 87 Fragments arranged by size: 1227 87 36 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 464 703 997 1350 Size: 464 239 294 353 Fragments arranged by size: 464 353 294 239 BsaI GGTCTCn'nnnn_ Cuts at: 0 1284 1350 Size: 1284 66 BsaAI yAC'GTr Cuts at: 0 411 914 1350 Size: 411 503 436 Fragments arranged by size: 503 436 411 BsaHI Gr'CG_yC Cuts at: 0 187 419 1350 Size: 187 232 931 Fragments arranged by size: 931 232 187 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 790 820 1350 Size: 790 30 530 Fragments arranged by size: 790 530 30 BseYI C'CCAG_C Cuts at: 0 157 705 1324 1350 Size: 157 548 619 26 Fragments arranged by size: 619 548 157 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1278 1350 Size: 1278 72 BsiEI CG_ry'CG Cuts at: 0 756 1350 Size: 756 594 BsiHKAI G_wGCw'C Cuts at: 0 119 410 449 808 1106 1350 Size: 119 291 39 359 298 244 Fragments arranged by size: 359 298 291 244 119 39 BsmBI CGTCTCn'nnnn_ Cuts at: 0 896 1350 Size: 896 454 Bsp1286I G_dGCh'C Cuts at: 0 119 134 410 449 569 808 853 1106 Size: 119 15 276 39 120 239 45 253 Cuts at: 1106 1193 1201 1349 1350 Size: 87 8 148 1 Fragments arranged by size: 276 253 239 148 120 119 87 45 39 15 8 1 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 690 1350 Size: 690 660 BsrBI CCG'CTC Cuts at: 0 291 1350 Size: 291 1059 BsrFI r'CCGG_y Cuts at: 0 183 544 653 713 877 1077 1350 Size: 183 361 109 60 164 200 273 Fragments arranged by size: 361 273 200 183 164 109 60 BsrGI T'GTAC_A Cuts at: 0 611 1350 Size: 611 739 BssSI C'ACGA_G Cuts at: 0 442 1350 Size: 442 908 BstEII G'GTnAC_C Cuts at: 0 13 1350 Size: 13 1337 BstYI r'GATC_y Cuts at: 0 950 1350 Size: 950 400 BtgI C'CryG_G Cuts at: 0 35 65 1193 1218 1274 1350 Size: 35 30 1128 25 56 76 Fragments arranged by size: 1128 76 56 35 30 25 BtsI GCAGTG_nn' Cuts at: 0 368 1235 1290 1350 Size: 368 867 55 60 Fragments arranged by size: 867 368 60 55 DraIII CAC_nnn'GTG Cuts at: 0 1230 1350 Size: 1230 120 EaeI y'GGCC_r Cuts at: 0 95 260 747 1221 1350 Size: 95 165 487 474 129 Fragments arranged by size: 487 474 165 129 95 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 798 1289 1350 Size: 798 491 61 Fragments arranged by size: 798 491 61 EcoICRI GAG'CTC Cuts at: 0 447 806 1350 Size: 447 359 544 Fragments arranged by size: 544 447 359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 464 703 907 997 1350 Size: 464 239 204 90 353 Fragments arranged by size: 464 353 239 204 90 EcoO109I rG'GnC_Cy Cuts at: 0 792 1189 1198 1286 1337 1345 1350 Size: 792 397 9 88 51 8 5 Fragments arranged by size: 792 397 88 51 9 8 5 EcoRI G'AATT_C Cuts at: 0 595 1350 Size: 595 755 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 855 1350 Size: 855 495 HaeII r_GCGC'y Cuts at: 0 190 1268 1350 Size: 190 1078 82 Fragments arranged by size: 1078 190 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 163 195 1035 1067 1350 Size: 163 32 840 32 283 Fragments arranged by size: 840 283 163 32 32 HincII GTy'rAC Cuts at: 0 1134 1350 Size: 1134 216 KasI G'GCGC_C Cuts at: 0 186 1350 Size: 186 1164 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 227 1350 Size: 227 1123 MscI TGG'CCA Cuts at: 0 1223 1350 Size: 1223 127 MslI CAynn'nnrTG Cuts at: 0 499 1350 Size: 499 851 MspA1I CmG'CkG Cuts at: 0 635 1140 1350 Size: 635 505 210 Fragments arranged by size: 635 505 210 NaeI GCC'GGC Cuts at: 0 185 1350 Size: 185 1165 NarI GG'CG_CC Cuts at: 0 187 1350 Size: 187 1163 NcoI C'CATG_G Cuts at: 0 65 1218 1274 1350 Size: 65 1153 56 76 Fragments arranged by size: 1153 76 65 56 NgoMIV G'CCGG_C Cuts at: 0 183 1350 Size: 183 1167 NspI r_CATG'y Cuts at: 0 329 1350 Size: 329 1021 PflMI CCAn_nnn'nTGG Cuts at: 0 1143 1275 1350 Size: 1143 132 75 Fragments arranged by size: 1143 132 75 PmlI CAC'GTG Cuts at: 0 411 1350 Size: 411 939 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 790 822 1350 Size: 790 32 528 Fragments arranged by size: 790 528 32 PpuMI rG'GwC_Cy Cuts at: 0 1286 1337 1350 Size: 1286 51 13 Fragments arranged by size: 1286 51 13 PshAI GACnn'nnGTC Cuts at: 0 418 1350 Size: 418 932 PspOMI G'GGCC_C Cuts at: 0 1189 1197 1345 1350 Size: 1189 8 148 5 Fragments arranged by size: 1189 148 8 5 PvuII CAG'CTG Cuts at: 0 635 1140 1350 Size: 635 505 210 Fragments arranged by size: 635 505 210 SacI G_AGCT'C Cuts at: 0 449 808 1350 Size: 449 359 542 Fragments arranged by size: 542 449 359 SanDI GG'GwC_CC Cuts at: 0 1286 1350 Size: 1286 64 SfcI C'TryA_G Cuts at: 0 1039 1057 1350 Size: 1039 18 293 Fragments arranged by size: 1039 293 18 SfoI GGC'GCC Cuts at: 0 188 1350 Size: 188 1162 SgrAI Cr'CCGG_yG Cuts at: 0 183 1350 Size: 183 1167 SmaI CCC'GGG Cuts at: 0 1203 1350 Size: 1203 147 StyI C'CwwG_G Cuts at: 0 65 870 1218 1274 1350 Size: 65 805 348 56 76 Fragments arranged by size: 805 348 76 65 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 151 430 770 1194 1350 Size: 151 279 340 424 156 Fragments arranged by size: 424 340 279 156 151 TatI w'GTAC_w Cuts at: 0 611 1350 Size: 611 739 Tth111I GACn'n_nGTC Cuts at: 0 538 1350 Size: 538 812 XmaI C'CCGG_G Cuts at: 0 1201 1350 Size: 1201 149 ZraI GAC'GTC Cuts at: 0 420 1350 Size: 420 930 Enzymes that do cut: AarI AatII AcuI AflIII AhdI AloI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbeI BglI BlpI Bme1580I BmrI BpmI BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstEII BstYI BtgI BtsI DraIII EaeI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PmlI PpiI PpuMI PshAI PspOMI PvuII SacI SanDI SfcI SfoI SgrAI SmaI StyI TaqII TatI Tth111I XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AleI AlfI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaBI BsaWI BseRI BsiWI BsmI BspEI BspHI BsrDI BssHII BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EcoNI EcoRV FseI FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PsiI PspXI PsrI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI XmnI