Restriction map for
pFN21AB7872
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# NO CUT ENZYMES ( 94 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AocI Aor51HI AscI AseI
AsnI Asp718I AviII AvrII BamHI
BbvCI BciVI BclI BfrI BglII
BlnI BsaBI BsaWI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DrdI EagI EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SalI
SapI ScaI SexAI SfiI SfuI
SmlI SnaBI SpeI SphI SspI
StuI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 63 enzymes) #
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AcsI 742 GAATTC [GA]^AATT[CT]
AflIII 1058 ACACGT A^C[GA][CT]GT
AhdI 319 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 742 GAATTC [GA]^AATT[CT]
Asp700I 131 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 319 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 682 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1368 TGGCCA TGG^CCA
BbeI 333 GGCGCC GGCGC^C
BbrPI 556 CACGTG CAC^GTG
BbsI 151 GTCTTC GAAGAC(2/6)
BcgI 77 GCACCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1688 CTAG C^TAG
BlpI 201 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 201 GCTGAGC GC^T[ACGT]AGC
BpuAI 151 GTCTTC GAAGAC(2/6)
BsaI 1425 GGTCTC GGTCTC(1/5)
BseRI 1882 GAGGAG GAGGAG(10/8)
BsgI 1440 CTGCAC GTGCAG(16/14)
BsiEI 900 CGACCG CG[GA][CT]^CG
BsmBI 1049 GAGACG CGTCTC(1/5)
Bsp1407I 758 TGTACA T^GTACA
BsrGI 758 TGTACA T^GTACA
BssSI 589 CACGAG CACGAG(-5/-1)
BstAPI 1671 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 201 GCTGAGC GC^T[ACGT]AGC
DraIII 1372 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 319 GACACCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1641 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 742 GAATTC G^AATTC
HgaI 704 GCGTC GACGC(5/10)
HincII 1279 GTCAAC GT[CT]^[GA]AC
HindII 1279 GTCAAC GT[CT]^[GA]AC
KasI 333 GGCGCC G^GCGCC
KspI 1794 CCGCGG CCGC^GG
MaeI 1688 CTAG C^TAG
MluNI 1368 TGGCCA TGG^CCA
MscI 1368 TGGCCA TGG^CCA
MseI 1489 TTAA T^TAA
MslI 642 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 330 GCCGGC GCC^GGC
NarI 333 GGCGCC GG^CGCC
NgoMIV 330 GCCGGC G^CCGGC
NspI 472 ACATGC [GA]CATG^[CT]
PflFI 682 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 556 CACGTG CAC^GTG
PmlI 556 CACGTG CAC^GTG
PshAI 561 GACCGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 1794 CCGCGG CCGC^GG
SbfI 1632 CCTGCAGG CCTGCA^GG
SfoI 333 GGCGCC GGC^GCC
SgrAI 329 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 1348 CCCGGG CCC^GGG
Sse8387I 1632 CCTGCAGG CCTGCA^GG
SspBI 758 TGTACA T^GTACA
TfiI 136 GATTC G^A[AT]TC
Tru9I 1489 TTAA T^TAA
Tsp509I 743 AATT ^AATT
Tth111I 682 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1571 CCATTGAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1348 CCCGGG C^CCGGG
XmaI 1348 CCCGGG C^CCGGG
XmnI 131 GAACGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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AatII 148 GACGTC GACGT^C
565 GACGTC
Alw44I 1249 GTGCAC G^TGCAC
1756 GTGCAC
ApaLI 1249 GTGCAC G^TGCAC
1756 GTGCAC
BglI 520 GCCATCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1802 GCCCAGGCGGC
BsaAI 556 CACGTG [CT]AC^GT[GA]
1059 CACGTA
BsmAI 1049 GAGAC GTCTC(1/5)
1426 GTCTC
BsrBI 436 CCGCTC CCGCTC(-3/-3)
1683 CCGCTC
DdeI 202 CTGAG C^T[ACGT]AG
1719 CTCAG
PflMI 1284 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1416 CCAGTCCATGG
PleI 1159 GACTC GAGTC(4/5)
1197 GAGTC
Van91I 1284 CCAGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1416 CCAGTCCATGG
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# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr10I Cfr13I DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII