(Circular) (Six-base) MAPSORT of: pFN21AB7872.seq Check: 7600 from: 1 to: 6613 pFN21AB7872 6613 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2842 3570 2842 Size: 728 5885 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2157 2574 4423 278 Size: 53 83 186 936 621 417 1849 2468 Fragments arranged by size: 2468 1849 936 621 417 186 83 53 AccI GT'mk_AC Cuts at: 1937 3956 1937 Size: 2019 4594 Acc65I G'GTAC_C Cuts at: 3934 3934 Size: 6613 AclI AA'CG_TT Cuts at: 1563 4740 5113 1563 Size: 3177 373 3063 Fragments arranged by size: 3177 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3059 3617 3668 4676 6060 6328 1632 Size: 1427 558 51 1008 1384 268 1917 Fragments arranged by size: 1917 1427 1384 1008 558 268 51 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6416 AflIII A'CryG_T Cuts at: 1203 3063 5512 6489 1203 Size: 1860 2449 977 1327 Fragments arranged by size: 2449 1860 1327 977 AgeI A'CCGG_T Cuts at: 5444 5444 Size: 6613 AhdI GACnn_n'nnGTC Cuts at: 2329 5342 2329 Size: 3013 3600 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6613 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6613 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2942 2974 2942 Size: 32 6581 AlwNI CAG_nnn'CTG Cuts at: 2522 2787 2857 2947 3157 3571 3646 3682 4356 Size: 265 70 90 210 414 75 36 674 Cuts at: 4356 5928 2522 Size: 1572 3207 Fragments arranged by size: 3207 1572 674 414 265 210 90 75 70 36 ApaI G_GGCC'C Cuts at: 3345 3353 3595 3345 Size: 8 242 6363 Fragments arranged by size: 6363 242 8 ApaLI G'TGCA_C Cuts at: 3254 3761 4670 5826 3254 Size: 507 909 1156 4041 Fragments arranged by size: 4041 1156 909 507 ApoI r'AATT_y Cuts at: 1493 2747 3922 4148 4300 6409 1493 Size: 1254 1175 226 152 2109 1697 Fragments arranged by size: 2109 1697 1254 1175 226 152 AseI AT'TA_AT Cuts at: 160 1032 5167 160 Size: 872 4135 1606 Fragments arranged by size: 4135 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6613 AvaI C'yCGr_G Cuts at: 1870 1943 2600 3353 3433 3605 3938 1870 Size: 73 657 753 80 172 333 4545 Fragments arranged by size: 4545 753 657 333 172 80 73 BamHI G'GATC_C Cuts at: 3943 4310 3943 Size: 367 6246 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 2338 2716 3026 3145 Size: 325 217 627 252 299 378 310 119 Cuts at: 3145 3934 5389 618 Size: 789 1455 1842 Fragments arranged by size: 1842 1455 789 627 378 325 310 299 252 217 119 BanII G_rGCy'C Cuts at: 1391 2066 2601 2960 3005 3345 3353 3595 3932 Size: 675 535 359 45 340 8 242 337 Cuts at: 3932 1391 Size: 4072 Fragments arranged by size: 4072 675 535 359 340 337 242 45 8 BbeI G_GCGC'C Cuts at: 2342 2342 Size: 6613 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2149 928 Size: 971 250 5392 Fragments arranged by size: 5392 971 250 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2071 2105 4804 4838 1903 Size: 34 134 34 2699 34 3678 Fragments arranged by size: 3678 2699 134 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4505 5715 4505 Size: 1210 5403 BclI T'GATC_A Cuts at: 1548 1548 Size: 6613 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2531 3813 5224 Size: 107 122 71 631 605 859 1282 1411 Cuts at: 5224 136 Size: 1525 Fragments arranged by size: 1525 1411 1282 859 631 605 122 107 71 BglII A'GATC_T Cuts at: 6608 6608 Size: 6613 BlpI GC'TnA_GC Cuts at: 2207 4030 2207 Size: 1823 4790 Bme1580I G_kGCm'C Cuts at: 1790 2042 2286 2721 3258 3345 3353 3595 3765 Size: 252 244 435 537 87 8 242 170 Cuts at: 3765 4674 5830 1790 Size: 909 1156 2573 Fragments arranged by size: 2573 1156 909 537 435 252 244 242 170 87 8 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6613 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2005 2095 2188 Size: 416 53 83 399 24 588 90 93 Cuts at: 2188 3415 4344 5302 442 Size: 1227 929 958 1753 Fragments arranged by size: 1753 1227 958 929 588 416 399 93 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6613 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2080 2186 2616 2855 3149 5273 1239 Size: 841 106 430 239 294 2124 2579 Fragments arranged by size: 2579 2124 841 430 294 239 106 Bpu10I CC'TnA_GC Cuts at: 3724 3724 Size: 6613 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4738 5603 5901 4738 Size: 865 298 5450 Fragments arranged by size: 5450 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3436 5276 882 Size: 617 1937 1840 2219 Fragments arranged by size: 2219 1937 1840 617 BsaAI yAC'GTr Cuts at: 493 1204 2563 3066 4404 493 Size: 711 1359 503 1338 2702 Fragments arranged by size: 2702 1359 1338 711 503 BsaBI GATnn'nnATC Cuts at: 6607 6607 Size: 6613 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2154 2339 2571 4420 Size: 53 83 186 936 621 185 232 1849 Cuts at: 4420 4802 5437 275 Size: 382 635 1451 Fragments arranged by size: 1849 1451 936 635 621 382 232 186 185 83 53 BsaWI w'CCGG_w Cuts at: 5046 5444 5718 5865 6276 5046 Size: 398 274 147 411 5383 Fragments arranged by size: 5383 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2942 2972 1178 Size: 30 165 30 1539 30 4819 Fragments arranged by size: 4819 1539 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3902 1186 Size: 2716 3897 BseYI C'CCAG_C Cuts at: 2309 2857 3476 5816 6312 2309 Size: 548 619 2340 496 2610 Fragments arranged by size: 2610 2340 619 548 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3430 1163 Size: 573 1694 4346 Fragments arranged by size: 4346 1694 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2908 4063 4824 5852 1540 Size: 275 186 907 1155 761 1028 2301 Fragments arranged by size: 2301 1155 1028 907 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2055 2066 2271 2562 2601 2960 3258 3765 4674 Size: 11 205 291 39 359 298 507 909 Cuts at: 4674 4759 5830 2055 Size: 85 1071 2838 Fragments arranged by size: 2838 1071 909 507 359 298 291 205 85 39 11 BsmI GAATG_Cn' Cuts at: 4124 4217 4124 Size: 93 6520 BsmBI CGTCTCn'nnnn_ Cuts at: 3048 3048 Size: 6613 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2055 2066 2271 2286 2562 2601 Size: 399 252 13 11 205 15 276 39 Cuts at: 2601 2721 2960 3005 3258 3345 3353 3595 3765 Size: 120 239 45 253 87 8 242 170 Cuts at: 3765 3932 4674 4759 5830 1391 Size: 167 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 276 253 252 242 239 205 170 167 120 87 85 45 39 15 13 11 8 BspEI T'CCGG_A Cuts at: 6276 6276 Size: 6613 BspHI T'CATG_A Cuts at: 4502 6232 4502 Size: 1730 4883 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2842 3570 3858 3968 844 Size: 1998 728 288 110 3489 Fragments arranged by size: 3489 1998 728 288 110 BsrBI CCG'CTC Cuts at: 1394 1823 2443 3690 4500 1394 Size: 429 620 1247 810 3507 Fragments arranged by size: 3507 1247 810 620 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5108 5282 65 Size: 1183 3860 174 1396 Fragments arranged by size: 3860 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2335 2696 2805 2865 3029 3229 5257 Size: 110 525 361 109 60 164 200 2028 Cuts at: 5257 5444 1700 Size: 187 2869 Fragments arranged by size: 2869 2028 525 361 200 187 164 110 109 60 BsrGI T'GTAC_A Cuts at: 96 2763 96 Size: 2667 3946 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6613 BssSI C'ACGA_G Cuts at: 2594 4673 5685 2594 Size: 2079 1012 3522 Fragments arranged by size: 3522 2079 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3682 794 Size: 2888 3725 BstBI TT'CG_AA Cuts at: 5417 5417 Size: 6613 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6613 BstYI r'GATC_y Cuts at: 1972 3102 3600 3906 3943 4310 4695 4712 6164 Size: 1130 498 306 37 367 385 17 1452 Cuts at: 6164 6250 6262 6608 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1130 498 385 367 346 306 86 37 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2217 3345 3370 3426 3799 513 Size: 552 939 213 1128 25 56 373 3327 Fragments arranged by size: 3327 1128 939 552 373 213 56 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5874 Fragments arranged by size: 5874 683 56 BtsI GCAGTG_nn' Cuts at: 2520 3387 3442 4129 4923 4943 2520 Size: 867 55 687 794 20 4190 Fragments arranged by size: 4190 867 794 687 55 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6613 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6578 DraI TTT'AAA Cuts at: 3917 3917 Size: 6613 DraIII CAC_nnn'GTG Cuts at: 3382 3382 Size: 6613 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5620 809 Size: 507 4304 1802 Fragments arranged by size: 4304 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2247 2412 2899 Size: 54 1425 50 275 18 417 165 487 Cuts at: 2899 3373 3702 4060 4949 6578 8 Size: 474 329 358 889 1629 43 Fragments arranged by size: 1629 1425 889 487 474 417 358 329 275 165 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4060 1537 Size: 275 2248 4090 Fragments arranged by size: 4090 2248 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4543 1353 Size: 3190 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2148 2950 3441 3848 5200 5574 Size: 1064 138 559 802 491 407 1352 374 Cuts at: 5574 5720 387 Size: 146 1280 Fragments arranged by size: 1352 1280 1064 802 559 491 407 374 146 138 EcoICRI GAG'CTC Cuts at: 2064 2599 2958 2064 Size: 535 359 5719 Fragments arranged by size: 5719 535 359 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2080 2186 2616 2855 3059 3149 3617 Size: 393 448 106 430 239 204 90 468 Cuts at: 3617 3668 4676 5273 6060 6328 1239 Size: 51 1008 597 787 268 1524 Fragments arranged by size: 1524 1008 787 597 468 448 430 393 268 239 204 106 90 51 EcoNI CCTnn'n_nnAGG Cuts at: 3650 3650 Size: 6613 EcoO109I rG'GnC_Cy Cuts at: 2944 3341 3350 3438 3583 3591 3592 3634 3643 Size: 397 9 88 145 8 1 42 9 Cuts at: 3643 2944 Size: 5914 Fragments arranged by size: 5914 397 145 88 42 9 9 8 1 EcoRI G'AATT_C Cuts at: 2747 3922 4300 2747 Size: 1175 378 5060 Fragments arranged by size: 5060 1175 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3007 3007 Size: 6613 FspI TGC'GCA Cuts at: 5119 5119 Size: 6613 HaeII r_GCGC'y Cuts at: 1678 2342 3420 3742 5760 1678 Size: 664 1078 322 2018 2531 Fragments arranged by size: 2531 2018 1078 664 322 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2315 2347 3187 3219 5254 Size: 32 135 32 743 32 840 32 2035 Cuts at: 5254 5286 5328 5360 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2035 840 743 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3286 3957 4203 669 Size: 988 281 1348 671 246 3079 Fragments arranged by size: 3079 1348 988 671 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6613 HpaI GTT'AAC Cuts at: 4203 4203 Size: 6613 KasI G'GCGC_C Cuts at: 2338 2338 Size: 6613 KpnI G_GTAC'C Cuts at: 3938 3938 Size: 6613 MfeI C'AATT_G Cuts at: 4212 4212 Size: 6613 MluI A'CGCG_T Cuts at: 6489 6489 Size: 6613 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2379 5727 5911 1305 Size: 1074 3348 184 2007 Fragments arranged by size: 3348 2007 1074 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3375 10 Size: 54 1425 343 1543 3248 Fragments arranged by size: 3248 1543 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2651 4571 4930 5089 518 Size: 648 1485 1920 359 159 2042 Fragments arranged by size: 2042 1920 1485 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2787 3292 3682 3745 3801 4029 4706 5854 Size: 1181 505 390 63 56 228 677 1148 Cuts at: 5854 6099 1606 Size: 245 2120 Fragments arranged by size: 2120 1181 1148 677 505 390 245 228 63 56 NaeI GCC'GGC Cuts at: 2337 2337 Size: 6613 NarI GG'CG_CC Cuts at: 2339 2339 Size: 6613 NcoI C'CATG_G Cuts at: 513 1065 2004 2217 3370 3426 513 Size: 552 939 213 1153 56 3700 Fragments arranged by size: 3700 1153 939 552 213 56 NdeI CA'TA_TG Cuts at: 387 387 Size: 6613 NgoMIV G'CCGG_C Cuts at: 2335 2335 Size: 6613 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6613 NotI GC'GGCC_GC Cuts at: 4060 4060 Size: 6613 NspI r_CATG'y Cuts at: 1132 1229 2481 3971 5516 1132 Size: 97 1252 1490 1545 2229 Fragments arranged by size: 2229 1545 1490 1252 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6613 PciI A'CATG_T Cuts at: 5512 5512 Size: 6613 PflMI CCAn_nnn'nTGG Cuts at: 1108 3295 3427 1108 Size: 2187 132 4294 Fragments arranged by size: 4294 2187 132 PmeI GTTT'AAAC Cuts at: 3917 3917 Size: 6613 PmlI CAC'GTG Cuts at: 2563 2563 Size: 6613 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2942 2974 4682 4714 6195 6227 2942 Size: 32 1708 32 1481 32 3328 Fragments arranged by size: 3328 1708 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3438 3583 3643 3438 Size: 145 60 6408 Fragments arranged by size: 6408 145 60 PshAI GACnn'nnGTC Cuts at: 1613 2570 1613 Size: 957 5656 PsiI TTA'TAA Cuts at: 4183 4183 Size: 6613 PspOMI G'GGCC_C Cuts at: 3341 3349 3591 3341 Size: 8 242 6363 Fragments arranged by size: 6363 242 8 PstI C_TGCA'G Cuts at: 830 3567 3642 3738 3965 830 Size: 2737 75 96 227 3478 Fragments arranged by size: 3478 2737 227 96 75 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6613 PvuII CAG'CTG Cuts at: 2787 3292 3682 2787 Size: 505 390 5718 Fragments arranged by size: 5718 505 390 SacI G_AGCT'C Cuts at: 2066 2601 2960 2066 Size: 535 359 5719 Fragments arranged by size: 5719 535 359 SacII CC_GC'GG Cuts at: 3802 3802 Size: 6613 SalI G'TCGA_C Cuts at: 1936 3955 1936 Size: 2019 4594 SanDI GG'GwC_CC Cuts at: 3438 3438 Size: 6613 SbfI CC_TGCA'GG Cuts at: 3642 3965 3642 Size: 323 6290 ScaI AGT'ACT Cuts at: 4861 6573 4861 Size: 1712 4901 SfcI C'TryA_G Cuts at: 826 1045 3191 3209 3563 3638 3664 3734 3961 Size: 219 2146 18 354 75 26 70 227 Cuts at: 3961 5096 5777 5968 826 Size: 1135 681 191 1471 Fragments arranged by size: 2146 1471 1135 681 354 227 219 191 75 70 26 18 SfoI GGC'GCC Cuts at: 2340 2340 Size: 6613 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6613 SgrAI Cr'CCGG_yG Cuts at: 1700 2335 1700 Size: 635 5978 SmaI CCC'GGG Cuts at: 1872 3355 3940 1872 Size: 1483 585 4545 Fragments arranged by size: 4545 1483 585 SmlI C'TyrA_G Cuts at: 820 1017 1943 4717 5618 5880 820 Size: 197 926 2774 901 262 1553 Fragments arranged by size: 2774 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4404 493 Size: 3911 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6613 SphI G_CATG'C Cuts at: 1132 3971 1132 Size: 2839 3774 SspI AAT'ATT Cuts at: 5 52 4537 5 Size: 47 4485 2081 Fragments arranged by size: 4485 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6613 StyI C'CwwG_G Cuts at: 513 1065 2004 2217 3022 3370 3426 3538 4052 Size: 552 939 213 805 348 56 112 514 Cuts at: 4052 513 Size: 3074 Fragments arranged by size: 3074 939 805 552 514 348 213 112 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2303 2582 2922 3346 4640 4657 4810 Size: 1255 723 279 340 424 1294 17 153 Cuts at: 4810 4995 5440 325 Size: 185 445 1498 Fragments arranged by size: 1498 1294 1255 723 445 424 340 279 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2763 4859 6571 Size: 274 80 33 51 1443 786 2096 1712 Cuts at: 6571 96 Size: 138 Fragments arranged by size: 2096 1712 1443 786 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6573 6605 6573 Size: 32 6581 Tth111I GACn'n_nGTC Cuts at: 1316 2690 1316 Size: 1374 5239 XbaI T'CTAG_A Cuts at: 3949 3949 Size: 6613 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3583 3583 Size: 6613 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6613 XmaI C'CCGG_G Cuts at: 1870 3353 3938 1870 Size: 1483 585 4545 Fragments arranged by size: 4545 1483 585 XmnI GAAnn'nnTTC Cuts at: 2140 4742 2140 Size: 2602 4011 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2155 2572 4421 276 Size: 53 83 186 936 621 417 1849 2468 Fragments arranged by size: 2468 1849 936 621 417 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI PsrI RsrII SapI SexAI SfiI SrfI SwaI