Restriction map for
pFN21AB7872
(Linear) (Six-base) MAPSORT of: KIBB7872.seq Check: 2226 from: 1 to: 1908
KIBB7872.orf
With 182 enzymes: SgfI *
July 15, 2010 18:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 837 1565 1908
Size: 837 728 343
Fragments arranged by size:
837 728 343
AatII G_ACGT'C
Cuts at: 0 152 569 1908
Size: 152 417 1339
Fragments arranged by size:
1339 417 152
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1054 1612 1663 1908
Size: 1054 558 51 245
Fragments arranged by size:
1054 558 245 51
AflIII A'CryG_T
Cuts at: 0 1058 1908
Size: 1058 850
AhdI GACnn_n'nnGTC
Cuts at: 0 324 1908
Size: 324 1584
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 937 969 1908
Size: 937 32 939
Fragments arranged by size:
939 937 32
AlwNI CAG_nnn'CTG
Cuts at: 0 517 782 852 942 1152 1566 1641 1677
Size: 517 265 70 90 210 414 75 36
Cuts at: 1677 1908
Size: 231
Fragments arranged by size:
517 414 265 231 210 90 75 70
36
ApaI G_GGCC'C
Cuts at: 0 1340 1348 1590 1908
Size: 1340 8 242 318
Fragments arranged by size:
1340 318 242 8
ApaLI G'TGCA_C
Cuts at: 0 1249 1756 1908
Size: 1249 507 152
Fragments arranged by size:
1249 507 152
ApoI r'AATT_y
Cuts at: 0 742 1908
Size: 742 1166
AvaI C'yCGr_G
Cuts at: 0 595 1348 1428 1600 1908
Size: 595 753 80 172 308
Fragments arranged by size:
753 595 308 172 80
BanI G'GyrC_C
Cuts at: 0 34 333 711 1021 1140 1908
Size: 34 299 378 310 119 768
Fragments arranged by size:
768 378 310 299 119 34
BanII G_rGCy'C
Cuts at: 0 61 596 955 1000 1340 1348 1590 1908
Size: 61 535 359 45 340 8 242 318
Fragments arranged by size:
535 359 340 318 242 61 45 8
BbeI G_GCGC'C
Cuts at: 0 337 1908
Size: 337 1571
BbsI GAAGACnn'nnnn_
Cuts at: 0 144 1908
Size: 144 1764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 66 100 1908
Size: 66 34 1808
Fragments arranged by size:
1808 66 34
BglI GCCn_nnn'nGGC
Cuts at: 0 526 1808 1908
Size: 526 1282 100
Fragments arranged by size:
1282 526 100
BlpI GC'TnA_GC
Cuts at: 0 202 1908
Size: 202 1706
Bme1580I G_kGCm'C
Cuts at: 0 37 281 716 1253 1340 1348 1590 1760
Size: 37 244 435 537 87 8 242 170
Cuts at: 1760 1908
Size: 148
Fragments arranged by size:
537 435 244 242 170 148 87 37
8
BmrI ACTGGGnnnn_n'
Cuts at: 0 90 183 1410 1908
Size: 90 93 1227 498
Fragments arranged by size:
1227 498 93 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 181 611 850 1144 1908
Size: 75 106 430 239 294 764
Fragments arranged by size:
764 430 294 239 106 75
Bpu10I CC'TnA_GC
Cuts at: 0 1719 1908
Size: 1719 189
BsaI GGTCTCn'nnnn_
Cuts at: 0 1431 1908
Size: 1431 477
BsaAI yAC'GTr
Cuts at: 0 558 1061 1908
Size: 558 503 847
Fragments arranged by size:
847 558 503
BsaHI Gr'CG_yC
Cuts at: 0 149 334 566 1908
Size: 149 185 232 1342
Fragments arranged by size:
1342 232 185 149
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 937 967 1908
Size: 937 30 941
Fragments arranged by size:
941 937 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1897 1908
Size: 1897 11
BseYI C'CCAG_C
Cuts at: 0 304 852 1471 1908
Size: 304 548 619 437
Fragments arranged by size:
619 548 437 304
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1425 1908
Size: 1425 483
BsiEI CG_ry'CG
Cuts at: 0 903 1908
Size: 903 1005
BsiHKAI G_wGCw'C
Cuts at: 0 50 61 266 557 596 955 1253 1760
Size: 50 11 205 291 39 359 298 507
Cuts at: 1760 1908
Size: 148
Fragments arranged by size:
507 359 298 291 205 148 50 39
11
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1043 1908
Size: 1043 865
Bsp1286I G_dGCh'C
Cuts at: 0 37 50 61 266 281 557 596 716
Size: 37 13 11 205 15 276 39 120
Cuts at: 716 955 1000 1253 1340 1348 1590 1760 1908
Size: 239 45 253 87 8 242 170 148
Fragments arranged by size:
276 253 242 239 205 170 148 120
87 45 39 37 15 13 11 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 837 1565 1853 1908
Size: 837 728 288 55
Fragments arranged by size:
837 728 288 55
BsrBI CCG'CTC
Cuts at: 0 438 1685 1908
Size: 438 1247 223
Fragments arranged by size:
1247 438 223
BsrFI r'CCGG_y
Cuts at: 0 330 691 800 860 1024 1224 1908
Size: 330 361 109 60 164 200 684
Fragments arranged by size:
684 361 330 200 164 109 60
BsrGI T'GTAC_A
Cuts at: 0 758 1908
Size: 758 1150
BssSI C'ACGA_G
Cuts at: 0 589 1908
Size: 589 1319
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1677 1908
Size: 1677 231
BstYI r'GATC_y
Cuts at: 0 1097 1595 1901 1908
Size: 1097 498 306 7
Fragments arranged by size:
1097 498 306 7
BtgI C'CryG_G
Cuts at: 0 212 1340 1365 1421 1794 1908
Size: 212 1128 25 56 373 114
Fragments arranged by size:
1128 373 212 114 56 25
BtsI GCAGTG_nn'
Cuts at: 0 515 1382 1437 1908
Size: 515 867 55 471
Fragments arranged by size:
867 515 471 55
DraIII CAC_nnn'GTG
Cuts at: 0 1377 1908
Size: 1377 531
EaeI y'GGCC_r
Cuts at: 0 242 407 894 1368 1697 1908
Size: 242 165 487 474 329 211
Fragments arranged by size:
487 474 329 242 211 165
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 143 945 1436 1843 1908
Size: 143 802 491 407 65
Fragments arranged by size:
802 491 407 143 65
EcoICRI GAG'CTC
Cuts at: 0 59 594 953 1908
Size: 59 535 359 955
Fragments arranged by size:
955 535 359 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 181 611 850 1054 1144 1612 1663
Size: 75 106 430 239 204 90 468 51
Cuts at: 1663 1908
Size: 245
Fragments arranged by size:
468 430 245 239 204 106 90 75
51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1645 1908
Size: 1645 263
EcoO109I rG'GnC_Cy
Cuts at: 0 939 1336 1345 1433 1578 1586 1587 1629
Size: 939 397 9 88 145 8 1 42
Cuts at: 1629 1638 1908
Size: 9 270
Fragments arranged by size:
939 397 270 145 88 42 9 9
8 1
EcoRI G'AATT_C
Cuts at: 0 742 1908
Size: 742 1166
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1002 1908
Size: 1002 906
HaeII r_GCGC'y
Cuts at: 0 337 1415 1737 1908
Size: 337 1078 322 171
Fragments arranged by size:
1078 337 322 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 1182 1214 1908
Size: 310 32 840 32 694
Fragments arranged by size:
840 694 310 32 32
HincII GTy'rAC
Cuts at: 0 1281 1908
Size: 1281 627
KasI G'GCGC_C
Cuts at: 0 333 1908
Size: 333 1575
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 1908
Size: 374 1534
MscI TGG'CCA
Cuts at: 0 1370 1908
Size: 1370 538
MslI CAynn'nnrTG
Cuts at: 0 646 1908
Size: 646 1262
MspA1I CmG'CkG
Cuts at: 0 782 1287 1677 1740 1796 1908
Size: 782 505 390 63 56 112
Fragments arranged by size:
782 505 390 112 63 56
NaeI GCC'GGC
Cuts at: 0 332 1908
Size: 332 1576
NarI GG'CG_CC
Cuts at: 0 334 1908
Size: 334 1574
NcoI C'CATG_G
Cuts at: 0 212 1365 1421 1908
Size: 212 1153 56 487
Fragments arranged by size:
1153 487 212 56
NgoMIV G'CCGG_C
Cuts at: 0 330 1908
Size: 330 1578
NspI r_CATG'y
Cuts at: 0 476 1908
Size: 476 1432
PflMI CCAn_nnn'nTGG
Cuts at: 0 1290 1422 1908
Size: 1290 132 486
Fragments arranged by size:
1290 486 132
PmlI CAC'GTG
Cuts at: 0 558 1908
Size: 558 1350
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 937 969 1908
Size: 937 32 939
Fragments arranged by size:
939 937 32
PpuMI rG'GwC_Cy
Cuts at: 0 1433 1578 1638 1908
Size: 1433 145 60 270
Fragments arranged by size:
1433 270 145 60
PshAI GACnn'nnGTC
Cuts at: 0 565 1908
Size: 565 1343
PspOMI G'GGCC_C
Cuts at: 0 1336 1344 1586 1908
Size: 1336 8 242 322
Fragments arranged by size:
1336 322 242 8
PstI C_TGCA'G
Cuts at: 0 1562 1637 1733 1908
Size: 1562 75 96 175
Fragments arranged by size:
1562 175 96 75
PvuII CAG'CTG
Cuts at: 0 782 1287 1677 1908
Size: 782 505 390 231
Fragments arranged by size:
782 505 390 231
SacI G_AGCT'C
Cuts at: 0 61 596 955 1908
Size: 61 535 359 953
Fragments arranged by size:
953 535 359 61
SacII CC_GC'GG
Cuts at: 0 1797 1908
Size: 1797 111
SanDI GG'GwC_CC
Cuts at: 0 1433 1908
Size: 1433 475
SbfI CC_TGCA'GG
Cuts at: 0 1637 1908
Size: 1637 271
SfcI C'TryA_G
Cuts at: 0 1186 1204 1558 1633 1659 1729 1908
Size: 1186 18 354 75 26 70 179
Fragments arranged by size:
1186 354 179 75 70 26 18
SfoI GGC'GCC
Cuts at: 0 335 1908
Size: 335 1573
SgrAI Cr'CCGG_yG
Cuts at: 0 330 1908
Size: 330 1578
SmaI CCC'GGG
Cuts at: 0 1350 1908
Size: 1350 558
StyI C'CwwG_G
Cuts at: 0 212 1017 1365 1421 1533 1908
Size: 212 805 348 56 112 375
Fragments arranged by size:
805 375 348 212 112 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 298 577 917 1341 1908
Size: 298 279 340 424 567
Fragments arranged by size:
567 424 340 298 279
TatI w'GTAC_w
Cuts at: 0 758 1908
Size: 758 1150
Tth111I GACn'n_nGTC
Cuts at: 0 685 1908
Size: 685 1223
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1578 1908
Size: 1578 330
XmaI C'CCGG_G
Cuts at: 0 1348 1908
Size: 1348 560
XmnI GAAnn'nnTTC
Cuts at: 0 135 1908
Size: 135 1773
ZraI GAC'GTC
Cuts at: 0 150 567 1908
Size: 150 417 1341
Fragments arranged by size:
1341 417 150
Enzymes that do cut:
AarI AatII AcuI AflIII AhdI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BcgI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstYI
BtgI BtsI DraIII EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PflMI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII
SacI SacII SanDI SbfI SfcI SfoI SgrAI SmaI
StyI TaqII TatI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AleI AlfI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BglII BmgBI BmtI BplI BpuEI BsaBI
BsaWI BsiWI BsmI BspEI BspHI BsrDI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EagI EarI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PsiI PspXI
PsrI PvuI RsrII SalI SapI ScaI SexAI SfiI
SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI XbaI XhoI