(Circular) (Six-base) MAPSORT of: pFN21AE5315.seq Check: 6132 from: 1 to: 5614 pFN21AE5315 5614 bp With 182 enzymes: SgfI * January 30, 2014 17:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2316 2764 2316 Size: 448 5166 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2244 3424 278 Size: 53 83 186 936 708 1180 2468 Fragments arranged by size: 2468 1180 936 708 186 83 53 AccI GT'mk_AC Cuts at: 1937 2957 1937 Size: 1020 4594 Acc65I G'GTAC_C Cuts at: 2935 2935 Size: 5614 AclI AA'CG_TT Cuts at: 1563 3741 4114 1563 Size: 2178 373 3063 Fragments arranged by size: 3063 2178 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2521 3677 5061 5329 1632 Size: 889 1156 1384 268 1917 Fragments arranged by size: 1917 1384 1156 889 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5417 AflIII A'CryG_T Cuts at: 1203 2810 2812 4513 5490 1203 Size: 1607 2 1701 977 1327 Fragments arranged by size: 1701 1607 1327 977 2 AgeI A'CCGG_T Cuts at: 4445 4445 Size: 5614 AhdI GACnn_n'nnGTC Cuts at: 4343 4343 Size: 5614 AleI CACnn'nnGTG Cuts at: 1166 2424 1166 Size: 1258 4356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2582 2669 1184 Size: 1398 87 4129 Fragments arranged by size: 4129 1398 87 AlwNI CAG_nnn'CTG Cuts at: 3357 4929 3357 Size: 1572 4042 ApaLI G'TGCA_C Cuts at: 3671 4827 3671 Size: 1156 4458 ApoI r'AATT_y Cuts at: 1493 2158 2923 3149 3301 5410 1493 Size: 665 765 226 152 2109 1697 Fragments arranged by size: 2109 1697 765 665 226 152 AseI AT'TA_AT Cuts at: 160 1032 4168 160 Size: 872 3136 1606 Fragments arranged by size: 3136 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 AvaI C'yCGr_G Cuts at: 1870 1943 2276 2636 2939 1870 Size: 73 333 360 303 4545 Fragments arranged by size: 4545 360 333 303 73 BamHI G'GATC_C Cuts at: 2944 3311 2944 Size: 367 5247 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2014 2069 2313 2566 2752 Size: 325 217 627 227 55 244 253 186 Cuts at: 2752 2935 4390 618 Size: 183 1455 1842 Fragments arranged by size: 1842 1455 627 325 253 244 227 217 186 183 55 BanII G_rGCy'C Cuts at: 1391 2576 2933 1391 Size: 1185 357 4072 Fragments arranged by size: 4072 1185 357 BbeI G_GCGC'C Cuts at: 2018 2018 Size: 5614 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2774 928 Size: 971 875 3768 Fragments arranged by size: 3768 971 875 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2560 2594 3805 3839 1903 Size: 34 623 34 1211 34 3678 Fragments arranged by size: 3678 1211 623 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3506 4716 3506 Size: 1210 4404 BclI T'GATC_A Cuts at: 1548 1548 Size: 5614 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2256 4225 136 Size: 107 122 71 631 605 584 1969 1525 Fragments arranged by size: 1969 1525 631 605 584 122 107 71 BglII A'GATC_T Cuts at: 5609 5609 Size: 5614 BlpI GC'TnA_GC Cuts at: 3031 3031 Size: 5614 Bme1580I G_kGCm'C Cuts at: 1790 2072 2105 2352 3675 4831 1790 Size: 282 33 247 1323 1156 2573 Fragments arranged by size: 2573 1323 1156 282 247 33 BmgBI CAC'GTC Cuts at: 1318 2382 1318 Size: 1064 4550 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3345 4303 442 Size: 416 53 83 399 24 1928 958 1753 Fragments arranged by size: 1928 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5614 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2173 2690 2798 4274 1239 Size: 917 17 517 108 1476 2579 Fragments arranged by size: 2579 1476 917 517 108 17 Bpu10I CC'TnA_GC Cuts at: 2219 2254 2219 Size: 35 5579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2825 3739 4604 4902 2825 Size: 914 865 298 3537 Fragments arranged by size: 3537 914 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2602 4277 882 Size: 617 1103 1675 2219 Fragments arranged by size: 2219 1675 1103 617 BsaAI yAC'GTr Cuts at: 493 1204 2065 2813 3405 493 Size: 711 861 748 592 2702 Fragments arranged by size: 2702 861 748 711 592 BsaBI GATnn'nnATC Cuts at: 5608 5608 Size: 5614 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2015 2202 2241 3421 Size: 53 83 186 936 482 187 39 1180 Cuts at: 3421 3803 4438 275 Size: 382 635 1451 Fragments arranged by size: 1451 1180 936 635 482 382 187 186 83 53 39 BsaWI w'CCGG_w Cuts at: 4047 4445 4719 4866 5277 4047 Size: 398 274 147 411 4384 Fragments arranged by size: 4384 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5389 Fragments arranged by size: 5389 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2510 2654 1186 Size: 967 357 144 4146 Fragments arranged by size: 4146 967 357 144 BseYI C'CCAG_C Cuts at: 2351 2511 2740 2821 4817 5313 2351 Size: 160 229 81 1996 496 2652 Fragments arranged by size: 2652 1996 496 229 160 81 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2863 1163 Size: 573 1127 3914 Fragments arranged by size: 3914 1127 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 2264 3064 3825 4853 1540 Size: 275 186 93 170 800 761 1028 2301 Fragments arranged by size: 2301 1028 800 761 275 186 170 93 BsiHKAI G_wGCw'C Cuts at: 3675 3760 4831 3675 Size: 85 1071 4458 Fragments arranged by size: 4458 1071 85 BsmI GAATG_Cn' Cuts at: 3125 3218 3125 Size: 93 5521 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2105 2352 2576 2933 3675 3760 Size: 399 282 33 247 224 357 742 85 Cuts at: 3760 4831 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 357 282 247 224 85 33 BspEI T'CCGG_A Cuts at: 5277 5277 Size: 5614 BspHI T'CATG_A Cuts at: 3503 5233 3503 Size: 1730 3884 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2316 2764 2969 844 Size: 1472 448 205 3489 Fragments arranged by size: 3489 1472 448 205 BsrBI CCG'CTC Cuts at: 1394 1823 2152 2253 3501 1394 Size: 429 329 101 1248 3507 Fragments arranged by size: 3507 1248 429 329 101 BsrDI GCAATG_nn' Cuts at: 65 1248 4109 4283 65 Size: 1183 2861 174 1396 Fragments arranged by size: 2861 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2017 2170 2259 2421 2437 4258 4445 Size: 110 207 153 89 162 16 1821 187 Cuts at: 4445 1700 Size: 2869 Fragments arranged by size: 2869 1821 207 187 162 153 110 89 16 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5614 BssHII G'CGCG_C Cuts at: 1925 2035 2037 2039 2110 1925 Size: 110 2 2 71 5429 Fragments arranged by size: 5429 110 71 2 2 BssSI C'ACGA_G Cuts at: 3674 4686 3674 Size: 1012 4602 BstAPI GCAn_nnn'nTGC Cuts at: 794 2457 794 Size: 1663 3951 BstBI TT'CG_AA Cuts at: 4418 4418 Size: 5614 BstEII G'GTnAC_C Cuts at: 1184 2629 1184 Size: 1445 4169 BstXI CCAn_nnnn'nTGG Cuts at: 2555 2626 2555 Size: 71 5543 BstYI r'GATC_y Cuts at: 1972 2788 2944 3311 3696 3713 5165 5251 5263 Size: 816 156 367 385 17 1452 86 12 Cuts at: 5263 5609 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 816 385 367 346 156 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2023 2023 Size: 5614 BtgI C'CryG_G Cuts at: 513 1065 2163 2733 2827 2908 513 Size: 552 1098 570 94 81 3219 Fragments arranged by size: 3219 1098 570 552 94 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4875 Fragments arranged by size: 4875 683 56 BtsI GCAGTG_nn' Cuts at: 3130 3924 3944 3130 Size: 794 20 4800 Fragments arranged by size: 4800 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5614 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5579 DraI TTT'AAA Cuts at: 2918 2918 Size: 5614 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2207 4621 809 Size: 507 891 2414 1802 Fragments arranged by size: 2414 1802 891 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2261 2286 Size: 54 1425 50 275 18 261 170 25 Cuts at: 2286 2319 2406 2730 3061 3950 5579 8 Size: 33 87 324 331 889 1629 43 Fragments arranged by size: 1629 1425 889 331 324 275 261 170 87 54 50 43 33 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2091 2261 3061 1537 Size: 275 279 170 800 4090 Fragments arranged by size: 4090 800 279 275 170 EarI CTCTTCn'nnn_ Cuts at: 1353 3544 1353 Size: 2191 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4201 4575 4721 387 Size: 1064 138 2612 374 146 1280 Fragments arranged by size: 2612 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2173 2521 2690 2798 3677 4274 Size: 393 524 17 348 169 108 879 597 Cuts at: 4274 5061 5329 1239 Size: 787 268 1524 Fragments arranged by size: 1524 879 787 597 524 393 348 268 169 108 17 EcoNI CCTnn'n_nnAGG Cuts at: 2138 2288 2138 Size: 150 5464 EcoO109I rG'GnC_Cy Cuts at: 2281 2293 2329 2281 Size: 12 36 5566 Fragments arranged by size: 5566 36 12 EcoRI G'AATT_C Cuts at: 2158 2923 3301 2158 Size: 765 378 4471 Fragments arranged by size: 4471 765 378 FseI GG_CCGG'CC Cuts at: 2263 2263 Size: 5614 FspI TGC'GCA Cuts at: 4120 4120 Size: 5614 HaeII r_GCGC'y Cuts at: 1678 2018 2251 2544 4761 1678 Size: 340 233 293 2217 2531 Fragments arranged by size: 2531 2217 340 293 233 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2669 2701 2773 2805 4255 Size: 32 135 32 1097 32 72 32 1450 Cuts at: 4255 4287 4329 4361 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1450 1097 135 72 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2958 3204 669 Size: 988 281 1020 246 3079 Fragments arranged by size: 3079 1020 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5614 HpaI GTT'AAC Cuts at: 3204 3204 Size: 5614 KasI G'GCGC_C Cuts at: 2014 2014 Size: 5614 KpnI G_GTAC'C Cuts at: 2939 2939 Size: 5614 MfeI C'AATT_G Cuts at: 3213 3213 Size: 5614 MluI A'CGCG_T Cuts at: 5490 5490 Size: 5614 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4728 4912 1305 Size: 3423 184 2007 Fragments arranged by size: 3423 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2408 10 Size: 54 1425 343 576 3216 Fragments arranged by size: 3216 1425 576 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2424 3572 3931 4090 518 Size: 648 1258 1148 359 159 2042 Fragments arranged by size: 2042 1258 1148 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2555 3030 3707 4855 5100 1606 Size: 949 475 677 1148 245 2120 Fragments arranged by size: 2120 1148 949 677 475 245 NaeI GCC'GGC Cuts at: 2019 2261 2019 Size: 242 5372 NarI GG'CG_CC Cuts at: 2015 2015 Size: 5614 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5062 NdeI CA'TA_TG Cuts at: 387 387 Size: 5614 NgoMIV G'CCGG_C Cuts at: 2017 2259 2017 Size: 242 5372 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5614 NotI GC'GGCC_GC Cuts at: 3061 3061 Size: 5614 NspI r_CATG'y Cuts at: 1132 1229 2972 4517 1132 Size: 97 1743 1545 2229 Fragments arranged by size: 2229 1743 1545 97 PasI CC'CwG_GG Cuts at: 1348 2794 1348 Size: 1446 4168 PciI A'CATG_T Cuts at: 4513 4513 Size: 5614 PflMI CCAn_nnn'nTGG Cuts at: 1108 2065 1108 Size: 957 4657 PfoI T'CCnGG_A Cuts at: 2384 2384 Size: 5614 PmeI GTTT'AAAC Cuts at: 2918 2918 Size: 5614 PmlI CAC'GTG Cuts at: 2813 2813 Size: 5614 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3683 3715 5196 5228 3683 Size: 32 1481 32 4069 Fragments arranged by size: 4069 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2281 2329 2281 Size: 48 5566 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5614 PsiI TTA'TAA Cuts at: 3184 3184 Size: 5614 PstI C_TGCA'G Cuts at: 830 2889 2966 830 Size: 2059 77 3478 Fragments arranged by size: 3478 2059 77 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5614 SalI G'TCGA_C Cuts at: 1936 2956 1936 Size: 1020 4594 SbfI CC_TGCA'GG Cuts at: 2889 2966 2889 Size: 77 5537 ScaI AGT'ACT Cuts at: 3862 5574 3862 Size: 1712 3902 SfcI C'TryA_G Cuts at: 826 1045 2885 2962 4097 4778 4969 826 Size: 219 1840 77 1135 681 191 1471 Fragments arranged by size: 1840 1471 1135 681 219 191 77 SfoI GGC'GCC Cuts at: 2016 2016 Size: 5614 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5614 SgrAI Cr'CCGG_yG Cuts at: 1700 2421 1700 Size: 721 4893 SmaI CCC'GGG Cuts at: 1872 2941 1872 Size: 1069 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2840 3718 4619 4881 820 Size: 197 926 897 878 901 262 1553 Fragments arranged by size: 1553 926 901 897 878 262 197 SnaBI TAC'GTA Cuts at: 493 3405 493 Size: 2912 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5614 SphI G_CATG'C Cuts at: 1132 2972 1132 Size: 1840 3774 SspI AAT'ATT Cuts at: 5 52 3538 5 Size: 47 3486 2081 Fragments arranged by size: 3486 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5614 StyI C'CwwG_G Cuts at: 513 1065 2625 3053 513 Size: 552 1560 428 3074 Fragments arranged by size: 3074 1560 552 428 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3641 3658 3811 3996 4441 325 Size: 1255 2061 17 153 185 445 1498 Fragments arranged by size: 2061 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2052 3860 5572 Size: 274 80 33 51 1443 75 1808 1712 Cuts at: 5572 96 Size: 138 Fragments arranged by size: 1808 1712 1443 274 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2851 2883 5574 5606 2851 Size: 32 2691 32 2859 Fragments arranged by size: 2859 2691 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5614 XbaI T'CTAG_A Cuts at: 2950 2950 Size: 5614 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5614 XmaI C'CCGG_G Cuts at: 1870 2939 1870 Size: 1069 4545 XmnI GAAnn'nnTTC Cuts at: 3743 3743 Size: 5614 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2242 3422 276 Size: 53 83 186 936 708 1180 2468 Fragments arranged by size: 2468 1180 936 708 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FspAI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI