Restriction map for
pFN21AE5315
(Linear) (Six-base) MAPSORT of: KIEE5315.seq Check: 1683 from: 1 to: 909
KIEE5315.orf
With 182 enzymes: SgfI *
January 29, 2014 11:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 311 759 909
Size: 311 448 150
Fragments arranged by size:
448 311 150
AatII G_ACGT'C
Cuts at: 0 239 909
Size: 239 670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 516 909
Size: 516 393
AflIII A'CryG_T
Cuts at: 0 805 807 909
Size: 805 2 102
Fragments arranged by size:
805 102 2
AleI CACnn'nnGTG
Cuts at: 0 419 909
Size: 419 490
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 577 664 909
Size: 577 87 245
Fragments arranged by size:
577 245 87
ApoI r'AATT_y
Cuts at: 0 153 909
Size: 153 756
AvaI C'yCGr_G
Cuts at: 0 271 631 909
Size: 271 360 278
Fragments arranged by size:
360 278 271
BanI G'GyrC_C
Cuts at: 0 9 64 308 561 747 909
Size: 9 55 244 253 186 162
Fragments arranged by size:
253 244 186 162 55 9
BanII G_rGCy'C
Cuts at: 0 571 909
Size: 571 338
BbeI G_GCGC'C
Cuts at: 0 13 909
Size: 13 896
BbsI GAAGACnn'nnnn_
Cuts at: 0 769 909
Size: 769 140
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 555 589 909
Size: 555 34 320
Fragments arranged by size:
555 320 34
BglI GCCn_nnn'nGGC
Cuts at: 0 251 909
Size: 251 658
Bme1580I G_kGCm'C
Cuts at: 0 67 100 347 909
Size: 67 33 247 562
Fragments arranged by size:
562 247 67 33
BmgBI CAC'GTC
Cuts at: 0 377 909
Size: 377 532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 168 685 793 909
Size: 151 17 517 108 116
Fragments arranged by size:
517 151 116 108 17
Bpu10I CC'TnA_GC
Cuts at: 0 214 249 909
Size: 214 35 660
Fragments arranged by size:
660 214 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 909
Size: 820 89
BsaI GGTCTCn'nnnn_
Cuts at: 0 597 909
Size: 597 312
BsaAI yAC'GTr
Cuts at: 0 60 808 909
Size: 60 748 101
Fragments arranged by size:
748 101 60
BsaHI Gr'CG_yC
Cuts at: 0 10 197 236 909
Size: 10 187 39 673
Fragments arranged by size:
673 187 39 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 505 649 909
Size: 148 357 144 260
Fragments arranged by size:
357 260 148 144
BseYI C'CCAG_C
Cuts at: 0 346 506 735 816 909
Size: 346 160 229 81 93
Fragments arranged by size:
346 229 160 93 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 858 909
Size: 858 51
BsiEI CG_ry'CG
Cuts at: 0 89 259 909
Size: 89 170 650
Fragments arranged by size:
650 170 89
Bsp1286I G_dGCh'C
Cuts at: 0 67 100 347 571 909
Size: 67 33 247 224 338
Fragments arranged by size:
338 247 224 67 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 311 759 909
Size: 311 448 150
Fragments arranged by size:
448 311 150
BsrBI CCG'CTC
Cuts at: 0 147 248 909
Size: 147 101 661
Fragments arranged by size:
661 147 101
BsrFI r'CCGG_y
Cuts at: 0 12 165 254 416 432 909
Size: 12 153 89 162 16 477
Fragments arranged by size:
477 162 153 89 16 12
BssHII G'CGCG_C
Cuts at: 0 30 32 34 105 909
Size: 30 2 2 71 804
Fragments arranged by size:
804 71 30 2 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 452 909
Size: 452 457
BstEII G'GTnAC_C
Cuts at: 0 624 909
Size: 624 285
BstXI CCAn_nnnn'nTGG
Cuts at: 0 550 621 909
Size: 550 71 288
Fragments arranged by size:
550 288 71
BstYI r'GATC_y
Cuts at: 0 783 909
Size: 783 126
Bsu36I CC'TnA_GG
Cuts at: 0 18 909
Size: 18 891
BtgI C'CryG_G
Cuts at: 0 158 728 822 903 909
Size: 158 570 94 81 6
Fragments arranged by size:
570 158 94 81 6
DrdI GACnn_nn'nnGTC
Cuts at: 0 202 909
Size: 202 707
EaeI y'GGCC_r
Cuts at: 0 86 256 281 314 401 725 909
Size: 86 170 25 33 87 324 184
Fragments arranged by size:
324 184 170 87 86 33 25
EagI C'GGCC_G
Cuts at: 0 86 256 909
Size: 86 170 653
Fragments arranged by size:
653 170 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 168 516 685 793 909
Size: 151 17 348 169 108 116
Fragments arranged by size:
348 169 151 116 108 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 133 283 909
Size: 133 150 626
Fragments arranged by size:
626 150 133
EcoO109I rG'GnC_Cy
Cuts at: 0 276 288 324 909
Size: 276 12 36 585
Fragments arranged by size:
585 276 36 12
EcoRI G'AATT_C
Cuts at: 0 153 909
Size: 153 756
FseI GG_CCGG'CC
Cuts at: 0 258 909
Size: 258 651
HaeII r_GCGC'y
Cuts at: 0 13 246 539 909
Size: 13 233 293 370
Fragments arranged by size:
370 293 233 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 664 696 768 800 909
Size: 664 32 72 32 109
Fragments arranged by size:
664 109 72 32 32
KasI G'GCGC_C
Cuts at: 0 9 909
Size: 9 900
MscI TGG'CCA
Cuts at: 0 403 909
Size: 403 506
MslI CAynn'nnrTG
Cuts at: 0 419 909
Size: 419 490
MspA1I CmG'CkG
Cuts at: 0 550 909
Size: 550 359
NaeI GCC'GGC
Cuts at: 0 14 256 909
Size: 14 242 653
Fragments arranged by size:
653 242 14
NarI GG'CG_CC
Cuts at: 0 10 909
Size: 10 899
NgoMIV G'CCGG_C
Cuts at: 0 12 254 909
Size: 12 242 655
Fragments arranged by size:
655 242 12
PasI CC'CwG_GG
Cuts at: 0 789 909
Size: 789 120
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 909
Size: 60 849
PfoI T'CCnGG_A
Cuts at: 0 379 909
Size: 379 530
PmlI CAC'GTG
Cuts at: 0 808 909
Size: 808 101
PpuMI rG'GwC_Cy
Cuts at: 0 276 324 909
Size: 276 48 585
Fragments arranged by size:
585 276 48
PstI C_TGCA'G
Cuts at: 0 884 909
Size: 884 25
SbfI CC_TGCA'GG
Cuts at: 0 884 909
Size: 884 25
SfcI C'TryA_G
Cuts at: 0 880 909
Size: 880 29
SfoI GGC'GCC
Cuts at: 0 11 909
Size: 11 898
SgrAI Cr'CCGG_yG
Cuts at: 0 416 909
Size: 416 493
SmlI C'TyrA_G
Cuts at: 0 835 909
Size: 835 74
StyI C'CwwG_G
Cuts at: 0 620 909
Size: 620 289
TatI w'GTAC_w
Cuts at: 0 47 909
Size: 47 862
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 846 878 909
Size: 846 32 31
Fragments arranged by size:
846 32 31
ZraI GAC'GTC
Cuts at: 0 237 909
Size: 237 672
Enzymes that do cut:
AarI AatII AcuI AflIII AleI AlfI ApoI AvaI
BanI BanII BbeI BbsI BcgI BglI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BseRI BseYI
BsgI BsiEI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BstEII BstXI BstYI Bsu36I BtgI DrdI EaeI EagI
Eco57MI EcoNI EcoO109I EcoRI FseI HaeII Hin4I KasI
MscI MslI MspA1I NaeI NarI NgoMIV PasI PflMI
PfoI PmlI PpuMI PstI SbfI SfcI SfoI SgrAI
SmlI StyI TatI TstI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmrI
BmtI BplI BsaBI BsaWI BsaXI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII EarI EciI
EcoICRI EcoRV FalI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MmeI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PmeI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SgfI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI XmnI