Restriction map for
pFN21AE5747
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# NO CUT ENZYMES ( 90 enzymes) #
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AclI AcsI AflII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AviII AvrII BamHI
BbrPI BbsI BciVI BclI BfrI
BglII BlnI BpuAI BsaAI BsaBI
BsiWI BspDI BspHI BspLU11I BsrDI
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI Eam1105I Eco47III
EcoRI EcoT22I FbaI FseI FspI
HindIII HpaI MamI MfeI MluI
MseI MunI NdeI NheI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SexAI SfiI SfuI
SmaI SnaBI SpeI SphI SspI
SwaI TfiI Tru9I Tsp509I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AatII 255 GACGTC GACGT^C
Acc65I 695 GGTACC G^GTACC
AccI 1510 GTCTAC GT^[AC][GT]AC
AccIII 824 TCCGGA T^CCGGA
AflIII 665 ACACGT A^C[GA][CT]GT
AlwI 570 GATCC GGATC(4/5)
ApaI 1885 GGGCCC GGGCC^C
Asp718I 695 GGTACC G^GTACC
AspI 252 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 498 CCTCAGC CCTCAGC(-5/-2)
BcgI 1653 CGATGCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BseAI 824 TCCGGA T^CCGGA
BsmBI 354 CGTCTC CGTCTC(1/5)
BsmI 1375 GCATTC GAATGC(1/-1)
Bsp1407I 485 TGTACA T^GTACA
BspEI 824 TCCGGA T^CCGGA
BspMI 1210 ACCTGC ACCTGC(4/8)
BsrBI 1530 CCGCTC CCGCTC(-3/-3)
BsrGI 485 TGTACA T^GTACA
BssSI 541 CACGAG CACGAG(-5/-1)
BstAPI 1586 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 390 GGTCACC G^GT[ACGT]ACC
BstPI 390 GGTCACC G^GT[ACGT]ACC
BstXI 215 CCATCCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 569 AGATCC [GA]^GATC[CT]
DpnII 570 GATC ^GATC
EarI 1870 GAAGAG CTCTTC(1/4)
EcoO65I 390 GGTCACC G^GT[ACGT]ACC
EcoRV 715 GATATC GAT^ATC
HincII 493 GTCAAC GT[CT]^[GA]AC
HindII 493 GTCAAC GT[CT]^[GA]AC
KpnI 695 GGTACC GGTAC^C
Ksp632I 1870 GAAGAG CTCTTC(1/4)
MboI 570 GATC ^GATC
MflI 569 AGATCC [GA]^GATC[CT]
MroI 824 TCCGGA T^CCGGA
MslI 1668 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1580 CCATGG C^CATGG
NdeII 570 GATC ^GATC
NotI 1682 GCGGCCGC GC^GGCCGC
PflFI 252 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Sau3AI 570 GATC ^GATC
ScaI 1256 AGTACT AGT^ACT
SgrAI 1113 CGCCGGCG C[GA]^CCGG[CT]G
SspBI 485 TGTACA T^GTACA
StuI 1169 AGGCCT AGG^CCT
Tth111I 252 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 569 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 34 enzymes) #
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Alw44I 361 GTGCAC G^TGCAC
1078 GTGCAC
AocI 921 CCTCAGG CC^T[ACGT]AGG
1733 CCTCAGG
ApaLI 361 GTGCAC G^TGCAC
1078 GTGCAC
AvaI 594 CTCGGG C^[CT]CG[GA]G
1087 CTCGGG
BalI 1436 TGGCCA TGG^CCA
1493 TGGCCA
BbeI 211 GGCGCC GGCGC^C
1545 GGCGCC
BfaI 1444 CTAG C^TAG
1678 CTAG
BsaI 1207 GAGACC GGTCTC(1/5)
1321 GAGACC
BsaWI 426 TCCGGT [AT]^CCGG[AT]
824 TCCGGA
BsgI 189 CTGCAC GTGCAG(16/14)
1107 CTGCAC
BsoBI 594 CTCGGG C^[CT]CG[GA]G
1087 CTCGGG
Bsu36I 921 CCTCAGG CC^T[ACGT]AGG
1733 CCTCAGG
DraIII 393 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
706 CACTATGTG
Eco81I 921 CCTCAGG CC^T[ACGT]AGG
1733 CCTCAGG
EcoT14I 122 CCAAGG C^C[AT][AT]GG
1580 CCATGG
KasI 211 GGCGCC G^GCGCC
1545 GGCGCC
KspI 234 CCGCGG CCGC^GG
734 CCGCGG
MaeI 1444 CTAG C^TAG
1678 CTAG
MboII 1870 GAAGA GAAGA(8/7)
185 TCTTC
MluNI 1436 TGGCCA TGG^CCA
1493 TGGCCA
MscI 1436 TGGCCA TGG^CCA
1493 TGGCCA
NarI 211 GGCGCC GG^CGCC
1545 GGCGCC
PflMI 705 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1311 CCAGGACCTGG
PpuMI 1313 AGGACCT [GA]G^G[AT]CC[CT]
1393 GGGACCT
SacI 1519 GAGCTC GAGCT^C
1633 GAGCTC
SacII 234 CCGCGG CCGC^GG
734 CCGCGG
SbfI 1361 CCTGCAGG CCTGCA^GG
1780 CCTGCAGG
SfoI 211 GGCGCC GGC^GCC
1545 GGCGCC
SmlI 247 CTCAAG C^T[CT][GA]AG
1897 CTTGAG
Sse8387I 1361 CCTGCAGG CCTGCA^GG
1780 CCTGCAGG
StyI 122 CCAAGG C^C[AT][AT]GG
1580 CCATGG
TaqI 866 TCGA T^CGA
1652 TCGA
TthHB8I 866 TCGA T^CGA
1652 TCGA
Van91I 705 CCACTATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1311 CCAGGACCTGG
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AspHI AvaII BanI BanII
BbvI BcnI BglI BlpI BmyI
BpmI Bpu1102I BsaHI BsaJI BseRI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstUI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MnlI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NgoMIV NlaIII NlaIV
PleI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI TseI Tsp45I
TspRI