(Circular) (Six-base) MAPSORT of: pFN21AE5747.seq Check: 6744 from: 1 to: 6619 pFN21AE5747 6619 bp With 182 enzymes: SgfI * December 8, 2014 13:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2264 4429 278 Size: 53 83 186 936 728 2165 2468 Fragments arranged by size: 2468 2165 936 728 186 83 53 AccI GT'mk_AC Cuts at: 1937 3516 3962 1937 Size: 1579 446 4594 Fragments arranged by size: 4594 1579 446 Acc65I G'GTAC_C Cuts at: 2700 3940 2700 Size: 1240 5379 AclI AA'CG_TT Cuts at: 1563 4746 5119 1563 Size: 3183 373 3063 Fragments arranged by size: 3183 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3016 4682 6066 6334 1632 Size: 1384 1666 1384 268 1917 Fragments arranged by size: 1917 1666 1384 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 2670 5518 6495 1203 Size: 1467 2848 977 1327 Fragments arranged by size: 2848 1467 1327 977 AgeI A'CCGG_T Cuts at: 5450 5450 Size: 6619 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 3677 1166 Size: 2511 4108 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6619 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2468 2500 2468 Size: 32 6587 AlwNI CAG_nnn'CTG Cuts at: 2590 3202 3322 3366 3463 3597 4362 5934 2590 Size: 612 120 44 97 134 765 1572 3275 Fragments arranged by size: 3275 1572 765 612 134 120 97 44 ApaI G_GGCC'C Cuts at: 3894 3894 Size: 6619 ApaLI G'TGCA_C Cuts at: 2366 3083 4676 5832 2366 Size: 717 1593 1156 3153 Fragments arranged by size: 3153 1593 1156 717 ApoI r'AATT_y Cuts at: 1493 3928 4154 4306 6415 1493 Size: 2435 226 152 2109 1697 Fragments arranged by size: 2435 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 2599 3092 3944 1870 Size: 73 656 493 852 4545 Fragments arranged by size: 4545 852 656 493 73 BamHI G'GATC_C Cuts at: 3949 4316 3949 Size: 367 6252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2136 2216 2337 2700 3021 Size: 325 217 627 349 80 121 363 321 Cuts at: 3021 3279 3550 3565 3940 5395 618 Size: 258 271 15 375 1455 1842 Fragments arranged by size: 1842 1455 627 375 363 349 325 321 271 258 217 121 80 15 BanII G_rGCy'C Cuts at: 1391 2060 2290 2319 2553 3101 3528 3642 3816 Size: 669 230 29 234 548 427 114 174 Cuts at: 3816 3894 3938 1391 Size: 78 44 4072 Fragments arranged by size: 4072 669 548 427 234 230 174 114 78 44 29 BbeI G_GCGC'C Cuts at: 2220 3554 2220 Size: 1334 5285 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5648 BbvCI CC'TCA_GC Cuts at: 2504 2504 Size: 6619 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3647 3681 4810 4844 1903 Size: 34 1710 34 1129 34 3678 Fragments arranged by size: 3678 1710 1129 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4511 5721 4511 Size: 1210 5409 BclI T'GATC_A Cuts at: 1548 1548 Size: 6619 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2092 2601 2772 Size: 107 122 71 631 605 420 509 171 Cuts at: 2772 3105 3743 3820 5230 136 Size: 333 638 77 1410 1525 Fragments arranged by size: 1525 1410 638 631 605 509 420 333 171 122 107 77 71 BglII A'GATC_T Cuts at: 6614 6614 Size: 6619 BlpI GC'TnA_GC Cuts at: 2010 2591 2889 4036 2010 Size: 581 298 1147 4593 Fragments arranged by size: 4593 1147 581 298 Bme1580I G_kGCm'C Cuts at: 1790 2139 2370 3024 3087 3894 4680 5836 1790 Size: 349 231 654 63 807 786 1156 2573 Fragments arranged by size: 2573 1156 807 786 654 349 231 63 BmgBI CAC'GTC Cuts at: 1318 2420 2673 3798 1318 Size: 1102 253 1125 4139 Fragments arranged by size: 4139 1125 1102 253 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3405 4350 5308 Size: 416 53 83 399 24 1988 945 958 Cuts at: 5308 442 Size: 1753 Fragments arranged by size: 1988 1753 958 945 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2540 3235 2540 Size: 695 5924 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2468 3344 3388 3656 3665 5279 1239 Size: 812 417 876 44 268 9 1614 2579 Fragments arranged by size: 2579 1614 876 812 417 268 44 9 Bpu10I CC'TnA_GC Cuts at: 2504 2504 Size: 6619 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2237 3923 4744 5609 5907 2237 Size: 1686 821 865 298 2949 Fragments arranged by size: 2949 1686 865 821 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3206 3320 5282 882 Size: 617 1707 114 1962 2219 Fragments arranged by size: 2219 1962 1707 617 114 BsaAI yAC'GTr Cuts at: 493 1204 4410 493 Size: 711 3206 2702 Fragments arranged by size: 3206 2702 711 BsaBI GATnn'nnATC Cuts at: 6613 6613 Size: 6619 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2084 2217 2261 2877 Size: 53 83 186 936 551 133 44 616 Cuts at: 2877 3551 3746 4426 4808 5443 275 Size: 674 195 680 382 635 1451 Fragments arranged by size: 1451 936 680 674 635 616 551 382 195 186 133 83 53 44 BsaWI w'CCGG_w Cuts at: 2431 2829 5052 5450 5724 5871 6282 2431 Size: 398 2223 398 274 147 411 2768 Fragments arranged by size: 2768 2223 411 398 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2277 2307 2468 2498 3143 Size: 30 165 30 874 30 161 30 645 Cuts at: 3143 3173 1178 Size: 30 4624 Fragments arranged by size: 4624 874 645 165 161 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2309 3502 3560 1186 Size: 1123 1193 58 4245 Fragments arranged by size: 4245 1193 1123 58 BseYI C'CCAG_C Cuts at: 2311 3815 3882 3887 3894 5822 6318 2311 Size: 1504 67 5 7 1928 496 2612 Fragments arranged by size: 2612 1928 1504 496 67 7 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2179 3097 1163 Size: 573 443 918 4685 Fragments arranged by size: 4685 918 573 443 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2426 2871 2998 3691 4069 4830 Size: 275 186 425 445 127 693 378 761 Cuts at: 4830 5858 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 693 445 425 378 275 186 127 BsiHKAI G_wGCw'C Cuts at: 2016 2370 3087 3528 3642 4680 4765 5836 2016 Size: 354 717 441 114 1038 85 1071 2799 Fragments arranged by size: 2799 1071 1038 717 441 354 114 85 BsmI GAATG_Cn' Cuts at: 3380 4130 4223 3380 Size: 750 93 5776 Fragments arranged by size: 5776 750 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2365 2365 Size: 6619 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2060 2139 2290 2319 2370 2553 Size: 399 226 44 79 151 29 51 183 Cuts at: 2553 3024 3087 3101 3528 3642 3816 3894 3938 Size: 471 63 14 427 114 174 78 44 Cuts at: 3938 4680 4765 5836 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 471 427 399 226 183 174 151 114 85 79 78 63 51 44 44 29 14 BspEI T'CCGG_A Cuts at: 2829 6282 2829 Size: 3453 3166 BspHI T'CATG_A Cuts at: 4508 6238 4508 Size: 1730 4889 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3224 3974 844 Size: 2380 750 3489 Fragments arranged by size: 3489 2380 750 BsrBI CCG'CTC Cuts at: 1394 1823 3537 4506 1394 Size: 429 1714 969 3507 Fragments arranged by size: 3507 1714 969 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5114 5288 65 Size: 1183 3866 174 1396 Fragments arranged by size: 3866 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2972 3087 3119 3247 3333 3693 5263 Size: 110 1162 115 32 128 86 360 1570 Cuts at: 5263 5450 1700 Size: 187 2869 Fragments arranged by size: 2869 1570 1162 360 187 128 115 110 86 32 BsrGI T'GTAC_A Cuts at: 96 2490 96 Size: 2394 4225 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 2546 4679 5691 2546 Size: 2133 1012 3474 Fragments arranged by size: 3474 2133 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3597 794 Size: 2803 3816 BstBI TT'CG_AA Cuts at: 5423 5423 Size: 6619 BstEII G'GTnAC_C Cuts at: 1184 2395 1184 Size: 1211 5408 BstXI CCAn_nnnn'nTGG Cuts at: 2227 2227 Size: 6619 BstYI r'GATC_y Cuts at: 1972 2574 3949 4316 4701 4718 6170 6256 6268 Size: 602 1375 367 385 17 1452 86 12 Cuts at: 6268 6614 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1375 602 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2927 3739 2927 Size: 812 5807 BtgI C'CryG_G Cuts at: 513 1065 2004 2212 2239 2739 2758 2908 3585 Size: 552 939 208 27 500 19 150 677 Cuts at: 3585 3833 513 Size: 248 3299 Fragments arranged by size: 3299 939 677 552 500 248 208 150 27 19 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5880 Fragments arranged by size: 5880 683 56 BtsI GCAGTG_nn' Cuts at: 3109 3295 4135 4929 4949 3109 Size: 186 840 794 20 4779 Fragments arranged by size: 4779 840 794 186 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6584 DraI TTT'AAA Cuts at: 3923 3923 Size: 6619 DraIII CAC_nnn'GTG Cuts at: 2403 2716 2403 Size: 313 6306 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5626 809 Size: 507 4310 1802 Fragments arranged by size: 4310 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2355 2423 2773 Size: 54 1425 50 275 18 525 68 350 Cuts at: 2773 2970 2995 3041 3106 3441 3498 3582 3688 Size: 197 25 46 65 335 57 84 106 Cuts at: 3688 4066 4955 6584 8 Size: 378 889 1629 43 Fragments arranged by size: 1629 1425 889 525 378 350 335 275 197 106 84 68 65 57 54 50 46 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 2423 2995 3688 4066 1537 Size: 275 611 572 693 378 4090 Fragments arranged by size: 4090 693 611 572 378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3870 4549 1353 Size: 2517 679 3423 Fragments arranged by size: 3423 2517 679 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2213 2429 2518 3406 5206 5580 Size: 1064 138 624 216 89 888 1800 374 Cuts at: 5580 5726 387 Size: 146 1280 Fragments arranged by size: 1800 1280 1064 888 624 374 216 146 138 89 EcoICRI GAG'CTC Cuts at: 3526 3640 3526 Size: 114 6505 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2468 3016 3344 3388 3656 3665 Size: 393 419 417 548 328 44 268 9 Cuts at: 3665 4682 5279 6066 6334 1239 Size: 1017 597 787 268 1524 Fragments arranged by size: 1524 1017 787 597 548 419 417 393 328 268 268 44 9 EcoNI CCTnn'n_nnAGG Cuts at: 3166 3314 3738 3166 Size: 148 424 6047 Fragments arranged by size: 6047 424 148 EcoO109I rG'GnC_Cy Cuts at: 3319 3363 3399 3615 3891 3319 Size: 44 36 216 276 6047 Fragments arranged by size: 6047 276 216 44 36 EcoRI G'AATT_C Cuts at: 3928 4306 3928 Size: 378 6241 EcoRV GAT'ATC Cuts at: 2722 2722 Size: 6619 FspI TGC'GCA Cuts at: 5125 5125 Size: 6619 HaeII r_GCGC'y Cuts at: 1678 2220 2896 3554 5766 1678 Size: 542 676 658 2212 2531 Fragments arranged by size: 2531 2212 676 658 542 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2277 2309 5260 5292 5334 Size: 32 135 32 705 32 2951 32 42 Cuts at: 5334 5366 1373 Size: 32 2626 Fragments arranged by size: 2951 2626 705 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2500 3963 4209 669 Size: 988 281 562 1463 246 3079 Fragments arranged by size: 3079 1463 988 562 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6619 HpaI GTT'AAC Cuts at: 4209 4209 Size: 6619 KasI G'GCGC_C Cuts at: 2216 3550 2216 Size: 1334 5285 KpnI G_GTAC'C Cuts at: 2704 3944 2704 Size: 1240 5379 MfeI C'AATT_G Cuts at: 4218 4218 Size: 6619 MluI A'CGCG_T Cuts at: 6495 6495 Size: 6619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5733 5917 1305 Size: 4428 184 2007 Fragments arranged by size: 4428 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3443 3500 10 Size: 54 1425 343 1611 57 3129 Fragments arranged by size: 3129 1611 1425 343 57 54 MslI CAynn'nnrTG Cuts at: 518 1166 3677 4577 4936 5095 518 Size: 648 2511 900 359 159 2042 Fragments arranged by size: 2511 2042 900 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2241 2311 2392 2741 2859 3202 3354 3428 Size: 635 70 81 349 118 343 152 74 Cuts at: 3428 3574 3819 4035 4712 5860 6105 1606 Size: 146 245 216 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 635 349 343 245 245 216 152 146 118 81 74 70 NaeI GCC'GGC Cuts at: 2974 3121 3249 3695 2974 Size: 147 128 446 5898 Fragments arranged by size: 5898 446 147 128 NarI GG'CG_CC Cuts at: 2217 3551 2217 Size: 1334 5285 NcoI C'CATG_G Cuts at: 513 1065 2004 3585 513 Size: 552 939 1581 3547 Fragments arranged by size: 3547 1581 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6619 NgoMIV G'CCGG_C Cuts at: 2972 3119 3247 3693 2972 Size: 147 128 446 5898 Fragments arranged by size: 5898 446 147 128 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 3688 4066 3688 Size: 378 6241 NspI r_CATG'y Cuts at: 1132 1229 3977 5522 1132 Size: 97 2748 1545 2229 Fragments arranged by size: 2748 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2281 1348 Size: 933 5686 PciI A'CATG_T Cuts at: 5518 5518 Size: 6619 PflMI CCAn_nnn'nTGG Cuts at: 1108 2716 3322 1108 Size: 1608 606 4405 Fragments arranged by size: 4405 1608 606 PfoI T'CCnGG_A Cuts at: 2172 2691 3633 3642 2172 Size: 519 942 9 5149 Fragments arranged by size: 5149 942 519 9 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2468 2500 4688 4720 6201 6233 2468 Size: 32 2188 32 1481 32 2854 Fragments arranged by size: 2854 2188 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3319 3399 3319 Size: 80 6539 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6619 PsiI TTA'TAA Cuts at: 4189 4189 Size: 6619 PspOMI G'GGCC_C Cuts at: 3890 3890 Size: 6619 PstI C_TGCA'G Cuts at: 830 2077 2586 2795 3269 3371 3602 3790 3971 Size: 1247 509 209 474 102 231 188 181 Cuts at: 3971 830 Size: 3478 Fragments arranged by size: 3478 1247 509 474 231 209 188 181 102 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 PvuII CAG'CTG Cuts at: 2311 2859 3202 3428 3574 2311 Size: 548 343 226 146 5356 Fragments arranged by size: 5356 548 343 226 146 SacI G_AGCT'C Cuts at: 3528 3642 3528 Size: 114 6505 SacII CC_GC'GG Cuts at: 2242 2742 2242 Size: 500 6119 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SbfI CC_TGCA'GG Cuts at: 3371 3790 3971 3371 Size: 419 181 6019 Fragments arranged by size: 6019 419 181 ScaI AGT'ACT Cuts at: 3263 4867 6579 3263 Size: 1604 1712 3303 Fragments arranged by size: 3303 1712 1604 SfcI C'TryA_G Cuts at: 826 1045 2073 2582 2791 3265 3367 3598 3786 Size: 219 1028 509 209 474 102 231 188 Cuts at: 3786 3967 5102 5783 5974 826 Size: 181 1135 681 191 1471 Fragments arranged by size: 1471 1135 1028 681 509 474 231 219 209 191 188 181 102 SfoI GGC'GCC Cuts at: 2218 3552 2218 Size: 1334 5285 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 3119 1700 Size: 1419 5200 SmaI CCC'GGG Cuts at: 1872 3946 1872 Size: 2074 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 3902 4723 5624 5886 820 Size: 197 926 309 1650 821 901 262 1553 Fragments arranged by size: 1650 1553 926 901 821 309 262 197 SnaBI TAC'GTA Cuts at: 493 4410 493 Size: 3917 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3977 1132 Size: 2845 3774 SspI AAT'ATT Cuts at: 5 52 4543 5 Size: 47 4491 2081 Fragments arranged by size: 4491 2081 47 StuI AGG'CCT Cuts at: 1517 3176 1517 Size: 1659 4960 StyI C'CwwG_G Cuts at: 513 1065 2004 2127 3585 4058 513 Size: 552 939 123 1458 473 3074 Fragments arranged by size: 3074 1458 939 552 473 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2140 2921 4646 4663 4816 5001 5446 Size: 1255 560 781 1725 17 153 185 445 Cuts at: 5446 325 Size: 1498 Fragments arranged by size: 1725 1498 1255 781 560 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2490 3261 3558 Size: 274 80 33 51 1443 513 771 297 Cuts at: 3558 4865 6577 96 Size: 1307 1712 138 Fragments arranged by size: 1712 1443 1307 771 513 297 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3143 3175 6579 6611 3143 Size: 32 3404 32 3151 Fragments arranged by size: 3404 3151 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2260 1316 Size: 944 5675 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 3944 1870 Size: 2074 4545 XmnI GAAnn'nnTTC Cuts at: 4748 4748 Size: 6619 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2262 4427 276 Size: 53 83 186 936 728 2165 2468 Fragments arranged by size: 2468 2165 936 728 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BstZ17I FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI