(Linear) (Six-base) MAPSORT of: KIEE5747.seq Check: 3889 from: 1 to: 1914 KIEE5747.orf With 182 enzymes: SgfI * December 5, 2014 13:09 .. AatII G_ACGT'C Cuts at: 0 259 1914 Size: 259 1655 AccI GT'mk_AC Cuts at: 0 1511 1914 Size: 1511 403 Acc65I G'GTAC_C Cuts at: 0 695 1914 Size: 695 1219 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1011 1914 Size: 1011 903 AflIII A'CryG_T Cuts at: 0 665 1914 Size: 665 1249 AleI CACnn'nnGTG Cuts at: 0 1672 1914 Size: 1672 242 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 463 495 1914 Size: 463 32 1419 Fragments arranged by size: 1419 463 32 AlwNI CAG_nnn'CTG Cuts at: 0 585 1197 1317 1361 1458 1592 1914 Size: 585 612 120 44 97 134 322 Fragments arranged by size: 612 585 322 134 120 97 44 ApaI G_GGCC'C Cuts at: 0 1889 1914 Size: 1889 25 ApaLI G'TGCA_C Cuts at: 0 361 1078 1914 Size: 361 717 836 Fragments arranged by size: 836 717 361 AvaI C'yCGr_G Cuts at: 0 594 1087 1914 Size: 594 493 827 Fragments arranged by size: 827 594 493 BanI G'GyrC_C Cuts at: 0 131 211 332 695 1016 1274 1545 1560 Size: 131 80 121 363 321 258 271 15 Cuts at: 1560 1914 Size: 354 Fragments arranged by size: 363 354 321 271 258 131 121 80 15 BanII G_rGCy'C Cuts at: 0 55 285 314 548 1096 1523 1637 1811 Size: 55 230 29 234 548 427 114 174 Cuts at: 1811 1889 1914 Size: 78 25 Fragments arranged by size: 548 427 234 230 174 114 78 55 29 25 BbeI G_GCGC'C Cuts at: 0 215 1549 1914 Size: 215 1334 365 Fragments arranged by size: 1334 365 215 BbvCI CC'TCA_GC Cuts at: 0 499 1914 Size: 499 1415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1642 1676 1914 Size: 1642 34 238 Fragments arranged by size: 1642 238 34 BglI GCCn_nnn'nGGC Cuts at: 0 87 596 767 1100 1738 1815 1914 Size: 87 509 171 333 638 77 99 Fragments arranged by size: 638 509 333 171 99 87 77 BlpI GC'TnA_GC Cuts at: 0 5 586 884 1914 Size: 5 581 298 1030 Fragments arranged by size: 1030 581 298 5 Bme1580I G_kGCm'C Cuts at: 0 134 365 1019 1082 1889 1914 Size: 134 231 654 63 807 25 Fragments arranged by size: 807 654 231 134 63 25 BmgBI CAC'GTC Cuts at: 0 415 668 1793 1914 Size: 415 253 1125 121 Fragments arranged by size: 1125 415 253 121 BmrI ACTGGGnnnn_n' Cuts at: 0 1400 1914 Size: 1400 514 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 535 1230 1914 Size: 535 695 684 Fragments arranged by size: 695 684 535 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 463 1339 1383 1651 1660 1914 Size: 46 417 876 44 268 9 254 Fragments arranged by size: 876 417 268 254 46 44 9 Bpu10I CC'TnA_GC Cuts at: 0 499 1914 Size: 499 1415 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 232 1914 Size: 232 1682 BsaI GGTCTCn'nnnn_ Cuts at: 0 1201 1315 1914 Size: 1201 114 599 Fragments arranged by size: 1201 599 114 BsaHI Gr'CG_yC Cuts at: 0 79 212 256 872 1546 1741 1914 Size: 79 133 44 616 674 195 173 Fragments arranged by size: 674 616 195 173 133 79 44 BsaWI w'CCGG_w Cuts at: 0 426 824 1914 Size: 426 398 1090 Fragments arranged by size: 1090 426 398 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 272 302 463 493 1138 1168 1914 Size: 272 30 161 30 645 30 746 Fragments arranged by size: 746 645 272 161 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 304 1497 1555 1914 Size: 304 1193 58 359 Fragments arranged by size: 1193 359 304 58 BseYI C'CCAG_C Cuts at: 0 306 1810 1877 1882 1889 1914 Size: 306 1504 67 5 7 25 Fragments arranged by size: 1504 306 67 25 7 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 174 1092 1914 Size: 174 918 822 Fragments arranged by size: 918 822 174 BsiEI CG_ry'CG Cuts at: 0 421 866 993 1686 1914 Size: 421 445 127 693 228 Fragments arranged by size: 693 445 421 228 127 BsiHKAI G_wGCw'C Cuts at: 0 11 365 1082 1523 1637 1914 Size: 11 354 717 441 114 277 Fragments arranged by size: 717 441 354 277 114 11 BsmI GAATG_Cn' Cuts at: 0 1375 1914 Size: 1375 539 BsmBI CGTCTCn'nnnn_ Cuts at: 0 360 1914 Size: 360 1554 Bsp1286I G_dGCh'C Cuts at: 0 11 55 134 285 314 365 548 1019 Size: 11 44 79 151 29 51 183 471 Cuts at: 1019 1082 1096 1523 1637 1811 1889 1914 Size: 63 14 427 114 174 78 25 Fragments arranged by size: 471 427 183 174 151 114 79 78 63 51 44 29 25 14 11 BspEI T'CCGG_A Cuts at: 0 824 1914 Size: 824 1090 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1219 1914 Size: 1219 695 BsrBI CCG'CTC Cuts at: 0 1532 1914 Size: 1532 382 BsrFI r'CCGG_y Cuts at: 0 967 1082 1114 1242 1328 1688 1914 Size: 967 115 32 128 86 360 226 Fragments arranged by size: 967 360 226 128 115 86 32 BsrGI T'GTAC_A Cuts at: 0 485 1914 Size: 485 1429 BssSI C'ACGA_G Cuts at: 0 541 1914 Size: 541 1373 BstAPI GCAn_nnn'nTGC Cuts at: 0 1592 1914 Size: 1592 322 BstEII G'GTnAC_C Cuts at: 0 390 1914 Size: 390 1524 BstXI CCAn_nnnn'nTGG Cuts at: 0 222 1914 Size: 222 1692 BstYI r'GATC_y Cuts at: 0 569 1914 Size: 569 1345 Bsu36I CC'TnA_GG Cuts at: 0 922 1734 1914 Size: 922 812 180 Fragments arranged by size: 922 812 180 BtgI C'CryG_G Cuts at: 0 207 234 734 753 903 1580 1828 1914 Size: 207 27 500 19 150 677 248 86 Fragments arranged by size: 677 500 248 207 150 86 27 19 BtsI GCAGTG_nn' Cuts at: 0 1104 1290 1914 Size: 1104 186 624 Fragments arranged by size: 1104 624 186 DraIII CAC_nnn'GTG Cuts at: 0 398 711 1914 Size: 398 313 1203 Fragments arranged by size: 1203 398 313 EaeI y'GGCC_r Cuts at: 0 350 418 768 965 990 1036 1101 1436 Size: 350 68 350 197 25 46 65 335 Cuts at: 1436 1493 1577 1683 1914 Size: 57 84 106 231 Fragments arranged by size: 350 350 335 231 197 106 84 68 65 57 46 25 EagI C'GGCC_G Cuts at: 0 418 990 1683 1914 Size: 418 572 693 231 Fragments arranged by size: 693 572 418 231 EarI CTCTTCn'nnn_ Cuts at: 0 1865 1914 Size: 1865 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 208 424 513 1401 1914 Size: 208 216 89 888 513 Fragments arranged by size: 888 513 216 208 89 EcoICRI GAG'CTC Cuts at: 0 1521 1635 1914 Size: 1521 114 279 Fragments arranged by size: 1521 279 114 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 46 463 1011 1339 1383 1651 1660 1914 Size: 46 417 548 328 44 268 9 254 Fragments arranged by size: 548 417 328 268 254 46 44 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 1161 1309 1733 1914 Size: 1161 148 424 181 Fragments arranged by size: 1161 424 181 148 EcoO109I rG'GnC_Cy Cuts at: 0 1314 1358 1394 1610 1886 1914 Size: 1314 44 36 216 276 28 Fragments arranged by size: 1314 276 216 44 36 28 EcoRV GAT'ATC Cuts at: 0 717 1914 Size: 717 1197 HaeII r_GCGC'y Cuts at: 0 215 891 1549 1914 Size: 215 676 658 365 Fragments arranged by size: 676 658 365 215 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 272 304 1914 Size: 272 32 1610 Fragments arranged by size: 1610 272 32 HincII GTy'rAC Cuts at: 0 495 1914 Size: 495 1419 KasI G'GCGC_C Cuts at: 0 211 1545 1914 Size: 211 1334 369 Fragments arranged by size: 1334 369 211 KpnI G_GTAC'C Cuts at: 0 699 1914 Size: 699 1215 MscI TGG'CCA Cuts at: 0 1438 1495 1914 Size: 1438 57 419 Fragments arranged by size: 1438 419 57 MslI CAynn'nnrTG Cuts at: 0 1672 1914 Size: 1672 242 MspA1I CmG'CkG Cuts at: 0 236 306 387 736 854 1197 1349 1423 Size: 236 70 81 349 118 343 152 74 Cuts at: 1423 1569 1814 1914 Size: 146 245 100 Fragments arranged by size: 349 343 245 236 152 146 118 100 81 74 70 NaeI GCC'GGC Cuts at: 0 969 1116 1244 1690 1914 Size: 969 147 128 446 224 Fragments arranged by size: 969 446 224 147 128 NarI GG'CG_CC Cuts at: 0 212 1546 1914 Size: 212 1334 368 Fragments arranged by size: 1334 368 212 NcoI C'CATG_G Cuts at: 0 1580 1914 Size: 1580 334 NgoMIV G'CCGG_C Cuts at: 0 967 1114 1242 1688 1914 Size: 967 147 128 446 226 Fragments arranged by size: 967 446 226 147 128 NotI GC'GGCC_GC Cuts at: 0 1683 1914 Size: 1683 231 PasI CC'CwG_GG Cuts at: 0 276 1914 Size: 276 1638 PflMI CCAn_nnn'nTGG Cuts at: 0 711 1317 1914 Size: 711 606 597 Fragments arranged by size: 711 606 597 PfoI T'CCnGG_A Cuts at: 0 167 686 1628 1637 1914 Size: 167 519 942 9 277 Fragments arranged by size: 942 519 277 167 9 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 463 495 1914 Size: 463 32 1419 Fragments arranged by size: 1419 463 32 PpuMI rG'GwC_Cy Cuts at: 0 1314 1394 1914 Size: 1314 80 520 Fragments arranged by size: 1314 520 80 PspOMI G'GGCC_C Cuts at: 0 1885 1914 Size: 1885 29 PstI C_TGCA'G Cuts at: 0 72 581 790 1264 1366 1597 1785 1914 Size: 72 509 209 474 102 231 188 129 Fragments arranged by size: 509 474 231 209 188 129 102 72 PvuII CAG'CTG Cuts at: 0 306 854 1197 1423 1569 1914 Size: 306 548 343 226 146 345 Fragments arranged by size: 548 345 343 306 226 146 SacI G_AGCT'C Cuts at: 0 1523 1637 1914 Size: 1523 114 277 Fragments arranged by size: 1523 277 114 SacII CC_GC'GG Cuts at: 0 237 737 1914 Size: 237 500 1177 Fragments arranged by size: 1177 500 237 SbfI CC_TGCA'GG Cuts at: 0 1366 1785 1914 Size: 1366 419 129 Fragments arranged by size: 1366 419 129 ScaI AGT'ACT Cuts at: 0 1258 1914 Size: 1258 656 SfcI C'TryA_G Cuts at: 0 68 577 786 1260 1362 1593 1781 1914 Size: 68 509 209 474 102 231 188 133 Fragments arranged by size: 509 474 231 209 188 133 102 68 SfoI GGC'GCC Cuts at: 0 213 1547 1914 Size: 213 1334 367 Fragments arranged by size: 1334 367 213 SgrAI Cr'CCGG_yG Cuts at: 0 1114 1914 Size: 1114 800 SmlI C'TyrA_G Cuts at: 0 247 1897 1914 Size: 247 1650 17 Fragments arranged by size: 1650 247 17 StuI AGG'CCT Cuts at: 0 1171 1914 Size: 1171 743 StyI C'CwwG_G Cuts at: 0 122 1580 1914 Size: 122 1458 334 Fragments arranged by size: 1458 334 122 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 135 916 1914 Size: 135 781 998 Fragments arranged by size: 998 781 135 TatI w'GTAC_w Cuts at: 0 485 1256 1553 1914 Size: 485 771 297 361 Fragments arranged by size: 771 485 361 297 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1138 1170 1914 Size: 1138 32 744 Fragments arranged by size: 1138 744 32 Tth111I GACn'n_nGTC Cuts at: 0 255 1914 Size: 255 1659 ZraI GAC'GTC Cuts at: 0 257 1914 Size: 257 1657 Enzymes that do cut: AatII AccI Acc65I AcuI AflIII AleI AloI AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI KpnI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII SbfI ScaI SfcI SfoI SgrAI SmlI StuI StyI TaqII TatI TstI Tth111I ZraI Enzymes that do not cut: AarI AclI AfeI AflII AgeI AhdI AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII BmtI BsaAI BsaBI BsiWI BspHI BsrDI BssHII BstBI BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI FalI FseI FspI FspAI HindIII HpaI MfeI MluI MmeI NdeI NheI NruI NsiI NspI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SapI SexAI SfiI SgfI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI XmaI XmnI