(Circular) (Six-base) MAPSORT of: pFN21AE3105.seq Check: 263 from: 1 to: 5482 pFN21AE3105 5482 bp With 182 enzymes: SgfI * July 4, 2020 18:17 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2408 3292 278 Size: 53 83 186 936 872 884 2468 Fragments arranged by size: 2468 936 884 872 186 83 53 AccI GT'mk_AC Cuts at: 1937 2825 1937 Size: 888 4594 Acc65I G'GTAC_C Cuts at: 2803 2803 Size: 5482 AclI AA'CG_TT Cuts at: 1563 3609 3982 1563 Size: 2046 373 3063 Fragments arranged by size: 3063 2046 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2075 2508 3545 4929 5197 1632 Size: 443 433 1037 1384 268 1917 Fragments arranged by size: 1917 1384 1037 443 433 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5285 AflIII A'CryG_T Cuts at: 1203 2313 4381 5358 1203 Size: 1110 2068 977 1327 Fragments arranged by size: 2068 1327 1110 977 AgeI A'CCGG_T Cuts at: 4313 4313 Size: 5482 AhdI GACnn_n'nnGTC Cuts at: 4211 4211 Size: 5482 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5482 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5482 AlwNI CAG_nnn'CTG Cuts at: 2053 3225 4797 2053 Size: 1172 1572 2738 Fragments arranged by size: 2738 1572 1172 ApaI G_GGCC'C Cuts at: 2384 2384 Size: 5482 ApaLI G'TGCA_C Cuts at: 3539 4695 3539 Size: 1156 4326 ApoI r'AATT_y Cuts at: 1493 2213 2791 3017 3169 5278 1493 Size: 720 578 226 152 2109 1697 Fragments arranged by size: 2109 1697 720 578 226 152 AseI AT'TA_AT Cuts at: 160 1032 4036 160 Size: 872 3004 1606 Fragments arranged by size: 3004 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 AvaI C'yCGr_G Cuts at: 1870 1943 2129 2807 1870 Size: 73 186 678 4545 Fragments arranged by size: 4545 678 186 73 BamHI G'GATC_C Cuts at: 2812 3179 2812 Size: 367 5115 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2089 2676 2696 2803 4258 Size: 325 217 627 302 587 20 107 1455 Cuts at: 4258 618 Size: 1842 Fragments arranged by size: 1842 1455 627 587 325 302 217 107 20 BanII G_rGCy'C Cuts at: 1391 2032 2112 2358 2384 2661 2801 1391 Size: 641 80 246 26 277 140 4072 Fragments arranged by size: 4072 641 277 246 140 80 26 BbeI G_GCGC'C Cuts at: 2700 2700 Size: 5482 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4511 BbvCI CC'TCA_GC Cuts at: 2762 2762 Size: 5482 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3673 3707 1903 Size: 34 1736 34 3678 Fragments arranged by size: 3678 1736 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3374 4584 3374 Size: 1210 4272 BclI T'GATC_A Cuts at: 1548 2244 1548 Size: 696 4786 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4093 136 Size: 107 122 71 631 605 2421 1525 Fragments arranged by size: 2421 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5477 5477 Size: 5482 BlpI GC'TnA_GC Cuts at: 2899 2899 Size: 5482 Bme1580I G_kGCm'C Cuts at: 1790 2384 3543 4699 1790 Size: 594 1159 1156 2573 Fragments arranged by size: 2573 1159 1156 594 BmgBI CAC'GTC Cuts at: 1318 2569 1318 Size: 1251 4231 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2226 2361 3213 Size: 416 53 83 399 24 809 135 852 Cuts at: 3213 4171 442 Size: 958 1753 Fragments arranged by size: 1753 958 852 809 416 399 135 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5482 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2645 4142 1239 Size: 1406 1497 2579 Fragments arranged by size: 2579 1497 1406 Bpu10I CC'TnA_GC Cuts at: 2360 2762 2360 Size: 402 5080 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3607 4472 4770 3607 Size: 865 298 4319 Fragments arranged by size: 4319 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4145 882 Size: 617 2646 2219 Fragments arranged by size: 2646 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2316 2758 3273 493 Size: 711 1112 442 515 2702 Fragments arranged by size: 2702 1112 711 515 442 BsaBI GATnn'nnATC Cuts at: 5476 5476 Size: 5482 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2405 2697 3289 3671 Size: 53 83 186 936 872 292 592 382 Cuts at: 3671 4306 275 Size: 635 1451 Fragments arranged by size: 1451 936 872 635 592 382 292 186 83 53 BsaWI w'CCGG_w Cuts at: 3915 4313 4587 4734 5145 3915 Size: 398 274 147 411 4252 Fragments arranged by size: 4252 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5257 Fragments arranged by size: 5257 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2513 2779 1186 Size: 1327 266 3889 Fragments arranged by size: 3889 1327 266 BseYI C'CCAG_C Cuts at: 4685 5181 4685 Size: 496 4986 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2456 1163 Size: 573 720 4189 Fragments arranged by size: 4189 720 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2932 3693 4721 1540 Size: 275 186 931 761 1028 2301 Fragments arranged by size: 2301 1028 931 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3543 3628 4699 3543 Size: 85 1071 4326 Fragments arranged by size: 4326 1071 85 BsmI GAATG_Cn' Cuts at: 2993 3086 2993 Size: 93 5389 BsmBI CGTCTCn'nnnn_ Cuts at: 2396 2579 2396 Size: 183 5299 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2112 2358 2384 2661 2801 3543 Size: 399 242 80 246 26 277 140 742 Cuts at: 3543 3628 4699 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 277 246 242 140 85 80 26 BspEI T'CCGG_A Cuts at: 5145 5145 Size: 5482 BspHI T'CATG_A Cuts at: 3371 5101 3371 Size: 1730 3752 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2837 844 Size: 1993 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3369 1394 Size: 429 1546 3507 Fragments arranged by size: 3507 1546 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3977 4151 65 Size: 1183 2729 174 1396 Fragments arranged by size: 2729 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2673 4126 4313 1700 Size: 110 863 1453 187 2869 Fragments arranged by size: 2869 1453 863 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5482 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5482 BssSI C'ACGA_G Cuts at: 3542 4554 3542 Size: 1012 4470 BstAPI GCAn_nnn'nTGC Cuts at: 794 2738 794 Size: 1944 3538 BstBI TT'CG_AA Cuts at: 2120 4286 2120 Size: 2166 3316 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5482 BstYI r'GATC_y Cuts at: 1972 2812 3179 3564 3581 5033 5119 5131 5477 Size: 840 367 385 17 1452 86 12 346 Cuts at: 5477 1972 Size: 1977 Fragments arranged by size: 1977 1452 840 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 2680 513 Size: 552 972 643 3315 Fragments arranged by size: 3315 972 643 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2755 487 Size: 683 56 1529 3214 Fragments arranged by size: 3214 1529 683 56 BtsI GCAGTG_nn' Cuts at: 2439 2998 3792 3812 2439 Size: 559 794 20 4109 Fragments arranged by size: 4109 794 559 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5482 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5447 DraI TTT'AAA Cuts at: 2786 2786 Size: 5482 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4489 809 Size: 507 3173 1802 Fragments arranged by size: 3173 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2929 3818 5447 Size: 54 1425 50 275 18 1099 889 1629 Cuts at: 5447 8 Size: 43 Fragments arranged by size: 1629 1425 1099 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2929 1537 Size: 275 1117 4090 Fragments arranged by size: 4090 1117 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3412 1353 Size: 2059 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4069 4443 4589 387 Size: 1064 138 2480 374 146 1280 Fragments arranged by size: 2480 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2508 2645 3545 4142 4929 5197 Size: 393 443 433 137 900 597 787 268 Cuts at: 5197 1239 Size: 1524 Fragments arranged by size: 1524 900 787 597 443 433 393 268 137 EcoNI CCTnn'n_nnAGG Cuts at: 2172 2172 Size: 5482 EcoO109I rG'GnC_Cy Cuts at: 2380 2381 2380 Size: 1 5481 EcoRI G'AATT_C Cuts at: 2791 3169 2791 Size: 378 5104 FspI TGC'GCA Cuts at: 3988 3988 Size: 5482 HaeII r_GCGC'y Cuts at: 1678 2700 4629 1678 Size: 1022 1929 2531 Fragments arranged by size: 2531 1929 1022 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4123 4155 4197 4229 1373 Size: 32 135 32 2551 32 42 32 2626 Fragments arranged by size: 2626 2551 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2826 3072 669 Size: 988 281 888 246 3079 Fragments arranged by size: 3079 988 888 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5482 HpaI GTT'AAC Cuts at: 3072 3072 Size: 5482 KasI G'GCGC_C Cuts at: 2696 2696 Size: 5482 KpnI G_GTAC'C Cuts at: 2807 2807 Size: 5482 MfeI C'AATT_G Cuts at: 3081 3081 Size: 5482 MluI A'CGCG_T Cuts at: 5358 5358 Size: 5482 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2057 2388 4596 4780 1305 Size: 752 331 2208 184 2007 Fragments arranged by size: 2208 2007 752 331 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3660 Fragments arranged by size: 3660 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3440 3799 3958 518 Size: 648 2274 359 159 2042 Fragments arranged by size: 2274 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2614 2898 3575 4723 4968 1606 Size: 1008 284 677 1148 245 2120 Fragments arranged by size: 2120 1148 1008 677 284 245 NarI GG'CG_CC Cuts at: 2697 2697 Size: 5482 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4930 NdeI CA'TA_TG Cuts at: 387 387 Size: 5482 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5482 NotI GC'GGCC_GC Cuts at: 2929 2929 Size: 5482 NspI r_CATG'y Cuts at: 1132 1229 2630 2840 4385 1132 Size: 97 1401 210 1545 2229 Fragments arranged by size: 2229 1545 1401 210 97 PasI CC'CwG_GG Cuts at: 1348 2389 1348 Size: 1041 4441 PciI A'CATG_T Cuts at: 4381 4381 Size: 5482 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5482 PmeI GTTT'AAAC Cuts at: 2786 2786 Size: 5482 PmlI CAC'GTG Cuts at: 2316 2316 Size: 5482 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3551 3583 5064 5096 3551 Size: 32 1481 32 3937 Fragments arranged by size: 3937 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5482 PsiI TTA'TAA Cuts at: 3052 3052 Size: 5482 PspOMI G'GGCC_C Cuts at: 2380 2380 Size: 5482 PstI C_TGCA'G Cuts at: 830 2834 830 Size: 2004 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5482 PvuII CAG'CTG Cuts at: 2614 2614 Size: 5482 SalI G'TCGA_C Cuts at: 1936 2824 1936 Size: 888 4594 SbfI CC_TGCA'GG Cuts at: 2834 2834 Size: 5482 ScaI AGT'ACT Cuts at: 3730 5442 3730 Size: 1712 3770 SfcI C'TryA_G Cuts at: 826 1045 2830 3965 4646 4837 826 Size: 219 1785 1135 681 191 1471 Fragments arranged by size: 1785 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2698 2698 Size: 5482 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5482 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5482 SmaI CCC'GGG Cuts at: 1872 2809 1872 Size: 937 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2129 3586 4487 4749 820 Size: 197 926 186 1457 901 262 1553 Fragments arranged by size: 1553 1457 926 901 262 197 186 SnaBI TAC'GTA Cuts at: 493 3273 493 Size: 2780 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5482 SphI G_CATG'C Cuts at: 1132 2630 2840 1132 Size: 1498 210 3774 Fragments arranged by size: 3774 1498 210 SspI AAT'ATT Cuts at: 5 52 2125 3406 5 Size: 47 2073 1281 2081 Fragments arranged by size: 2081 2073 1281 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5482 StyI C'CwwG_G Cuts at: 513 1065 2921 513 Size: 552 1856 3074 Fragments arranged by size: 3074 1856 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2681 3509 3526 3679 3864 4309 325 Size: 1255 1101 828 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1101 828 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3728 5440 96 Size: 274 80 33 51 1443 1751 1712 138 Fragments arranged by size: 1751 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5442 5474 5442 Size: 32 5450 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5482 XbaI T'CTAG_A Cuts at: 2818 2818 Size: 5482 XhoI C'TCGA_G Cuts at: 1943 2129 1943 Size: 186 5296 XmaI C'CCGG_G Cuts at: 1870 2807 1870 Size: 937 4545 XmnI GAAnn'nnTTC Cuts at: 2481 3611 2481 Size: 1130 4352 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2406 3290 276 Size: 53 83 186 936 872 884 2468 Fragments arranged by size: 2468 936 884 872 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI