################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AhdI AocI Aor51HI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbsI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HindIII HpaI KpnI Ksp632I MamI MfeI MflI MluI MroI MseI MslI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfuI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 757 GACGTC GACGT^C AgeI 797 ACCGGT A^CCGGT Alw44I 673 GTGCAC G^TGCAC AlwI 225 GATCC GGATC(4/5) ApaI 254 GGGCCC GGGCC^C ApaLI 673 GTGCAC G^TGCAC AscI 317 GGCGCGCC GG^CGCGCC BbvCI 687 CCTCAGC CCTCAGC(-5/-2) BfaI 40 CTAG C^TAG BsaWI 797 ACCGGT [AT]^CCGG[AT] BseRI 310 GAGGAG GAGGAG(10/8) BsgI 910 GTGCAG GTGCAG(16/14) BsmAI 729 GTCTC GTCTC(1/5) BstAPI 675 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 981 CATCC GGATG(2/0) BstXI 401 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 688 CTCAG C^T[ACGT]AG DpnII 225 GATC ^GATC EcoT14I 401 CCAAGG C^C[AT][AT]GG FokI 981 CATCC GGATG(9/13) FseI 294 GGCCGGCC GGCCGG^CC HinfI 472 GACTC G^A[ACGT]TC HphI 828 GGTGA GGTGA(8/7) MaeI 40 CTAG C^TAG MaeIII 829 GTGAC ^GT[ACGT]AC MboI 225 GATC ^GATC MboII 554 TCTTC GAAGA(8/7) NaeI 295 GCCGGC GCC^GGC NdeII 225 GATC ^GATC NgoMIV 295 GCCGGC G^CCGGC NotI 566 GCGGCCGC GC^GGCCGC NspI 854 ACATGC [GA]CATG^[CT] PflMI 819 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 797 ACCGGT A^CCGGT PleI 472 GACTC GAGTC(4/5) PstI 810 CTGCAG CTGCA^G PvuII 913 CAGCTG CAG^CTG Sau3AI 225 GATC ^GATC SfaNI 980 GCATC GCATC(5/9) SfcI 810 CTGCAG C^T[GA][CT]AG SfiI 411 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 749 CGCCGGTG C[GA]^CCGG[CT]G SmlI 457 CTCAAG C^T[CT][GA]AG StuI 42 AGGCCT AGG^CCT StyI 401 CCAAGG C^C[AT][AT]GG Tsp45I 829 GTGAC ^GT[GC]AC Van91I 819 CCAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 822 GTAC GT^AC 933 GTAC AlwNI 820 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG 841 CAGAGCCTG BalI 188 TGGCCA TGG^CCA 410 TGGCCA BbrPI 234 CACGTG CAC^GTG 436 CACGTG BsrBI 313 GAGCGG CCGCTC(-3/-3) 328 GAGCGG KspI 564 CCGCGG CCGC^GG 804 CCGCGG MluNI 188 TGGCCA TGG^CCA 410 TGGCCA MscI 188 TGGCCA TGG^CCA 410 TGGCCA NlaIII 414 CATG CATG^ 855 CATG PmaCI 234 CACGTG CAC^GTG 436 CACGTG PmlI 234 CACGTG CAC^GTG 436 CACGTG PpuMI 110 GGGACCC [GA]G^G[AT]CC[CT] 350 GGGACCT RsaI 822 GTAC GT^AC 933 GTAC SacII 564 CCGCGG CCGC^GG 804 CCGCGG SexAI 668 ACCTGGT A^CC[AT]GGT 824 ACCTGGT SmaI 106 CCCGGG CCC^GGG 258 CCCGGG TaqI 446 TCGA T^CGA 785 TCGA TspRI 179 CAGTG CA[GC]TG[ACGT][ACGT]^ 958 CAGTG TthHB8I 446 TCGA T^CGA 785 TCGA XmaCI 106 CCCGGG C^CCGGG 258 CCCGGG XmaI 106 CCCGGG C^CCGGG 258 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AcyI AflIII AluI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BglI BmyI BpmI BsaAI BsaHI BsaJI BsiEI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI KasI MaeII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIV Sau96I ScrFI SfoI TseI